FastQCFastQC Report
Thu 26 May 2016
SRR937495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2448301
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118200.4827837753609544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93670.3825918463456903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61830.25254247741597136No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.1051749764428475No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25400.1037454136562457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87750.034.5515821
GTATCAA134400.032.7774281
ATCAACG163700.026.6358533
TCAACGC164850.026.4500394
TATCAAC168050.026.1442282
CAACGCA168650.025.854075
AACGCAG172400.025.2365976
GTACATG100900.022.9367681
TACATGG101900.022.2805712
ACGCAGA195000.022.2404847
CGCAGAG196300.022.04488
ACATGGG102150.021.575073
AGTACTT111200.021.46541812-13
GAGTACT110950.020.9572112-13
AGAGTAC162100.020.93761810-11
GTACTTT111700.020.66596414-15
GCAGAGT208350.020.313859
TACTTTT125700.018.74213214-15
CATGGGA51800.017.7887134
ACTTTTT133200.017.47305716-17
Produced by FastQC (version 0.11.5)