##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937495_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2448301 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.175656506287424 33.0 31.0 34.0 31.0 34.0 2 32.39261920817743 34.0 31.0 34.0 31.0 34.0 3 32.454782316390016 34.0 31.0 34.0 31.0 34.0 4 36.089355843092825 37.0 35.0 37.0 35.0 37.0 5 36.006188373079944 37.0 35.0 37.0 35.0 37.0 6 36.037548079259864 37.0 35.0 37.0 35.0 37.0 7 35.97730466964642 37.0 35.0 37.0 35.0 37.0 8 35.949913429762105 37.0 35.0 37.0 35.0 37.0 9 37.64070226659222 39.0 37.0 39.0 35.0 39.0 10-11 37.557251334701085 39.0 37.0 39.0 35.0 39.0 12-13 37.4488606588814 39.0 37.0 39.0 34.5 39.0 14-15 38.659308843152864 40.0 38.0 41.0 34.0 41.0 16-17 38.61677730801891 40.0 38.0 41.0 34.0 41.0 18-19 38.610125348149595 40.0 38.0 41.0 34.0 41.0 20-21 38.50422170313209 40.0 38.0 41.0 34.0 41.0 22-23 38.4467555255665 40.0 38.0 41.0 34.0 41.0 24-25 38.480611861041595 40.0 38.0 41.0 34.0 41.0 26-27 38.32357949451477 40.0 38.0 41.0 34.0 41.0 28-29 38.17553213432498 40.0 38.0 41.0 34.0 41.0 30-31 38.22433311917121 40.0 38.0 41.0 34.0 41.0 32-33 38.12211100677572 40.0 37.5 41.0 34.0 41.0 34-35 37.98302496302538 40.0 37.0 41.0 33.0 41.0 36-37 37.818913197356046 40.0 37.0 41.0 33.0 41.0 38-39 37.70402577134102 40.0 37.0 41.0 33.0 41.0 40-41 37.512969810493075 40.0 37.0 41.0 33.0 41.0 42-43 37.309921043205065 40.0 37.0 41.0 32.5 41.0 44-45 37.007282397058205 40.0 36.0 41.0 31.0 41.0 46-47 36.64279432961878 39.0 36.0 41.0 30.5 41.0 48-49 36.3133515854464 39.0 36.0 41.0 30.0 41.0 50-51 36.06371316271978 39.0 35.5 40.5 29.5 41.0 52-53 35.82019122648727 39.0 35.0 40.0 29.0 41.0 54-55 35.60441628704967 39.0 35.0 40.0 28.0 41.0 56-57 35.29346963465685 38.0 35.0 40.0 27.5 41.0 58-59 35.02822692144471 38.0 34.0 40.0 27.0 41.0 60-61 34.67751555057977 38.0 34.0 40.0 26.0 41.0 62-63 34.305566390733816 37.5 34.0 40.0 26.0 41.0 64-65 33.88853841909145 37.0 33.0 39.5 25.0 41.0 66-67 33.43782218771303 36.0 33.0 39.0 23.5 40.0 68-69 32.97807377442561 36.0 32.0 39.0 22.5 40.0 70-71 32.53104928683197 35.0 32.0 38.0 22.0 40.0 72-73 32.03073825481426 35.0 31.0 37.5 20.5 39.5 74-75 31.537441474720634 35.0 31.0 37.0 19.5 39.0 76-77 30.224411336677967 33.5 29.5 35.5 18.5 38.0 78-79 30.874208481718547 34.0 30.5 36.0 18.5 38.0 80-81 30.677657689965407 34.0 30.5 36.0 18.0 37.5 82-83 30.26489655479453 34.0 30.0 35.0 14.5 37.0 84-85 29.819865490395173 34.0 30.0 35.0 8.5 36.5 86-87 29.427343288263984 34.0 29.0 35.0 6.5 36.0 88-89 28.950576542671836 34.0 29.0 35.0 2.0 36.0 90-91 28.378852722765707 33.5 28.5 35.0 2.0 35.0 92-93 27.843290714662942 33.0 27.0 35.0 2.0 35.0 94-95 27.32169941522713 33.0 26.5 35.0 2.0 35.0 96-97 26.620989412658002 32.5 25.0 34.0 2.0 35.0 98-99 25.602809254254275 32.0 23.5 34.0 2.0 35.0 100-101 22.64535467656959 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09992670168526274 1101 2 0.18291061151806787 1101 3 0.12069886015651576 1101 4 0.04300983659140911 1101 5 0.09776654696320008 1101 6 0.13620954974141597 1101 7 0.12124571414701535 1101 8 0.12097805771960424 1101 9 0.12601145260214963 1101 10-11 0.14365828404854852 1101 12-13 0.19049341834959677 1101 14-15 0.20795988032889312 1101 16-17 0.14856205831762992 1101 18-19 0.1732253680869249 1101 20-21 0.2235292753188105 1101 22-23 0.21338210246856448 1101 24-25 0.23039188390287535 1101 26-27 0.03723283935198651 1101 28-29 -0.0036564129632807862 1101 30-31 0.2027577162537142 1101 32-33 0.18428684107355764 1101 34-35 0.21205195062432125 1101 36-37 0.2661293016924233 1101 38-39 0.2575653685781134 1101 40-41 0.20282527158863672 1101 42-43 0.2734720763012888 1101 44-45 0.32716896544324214 1101 46-47 0.4461904888997026 1101 48-49 0.39498501732720825 1101 50-51 0.4607805358933703 1101 52-53 0.402846644999002 1101 54-55 0.38921959782405224 1101 56-57 0.47223154460893824 1101 58-59 0.4675933936721677 1101 60-61 0.4501126311473129 1101 62-63 0.4812561390888206 1101 64-65 0.5085907958699991 1101 66-67 0.4596442746954068 1101 68-69 0.4407232196559505 1101 70-71 0.4582232806554387 1101 72-73 0.4391739513871542 1101 74-75 0.4781431433840062 1101 76-77 0.4792436368284889 1101 78-79 0.5634838897806063 1101 80-81 0.5373960843264385 1101 82-83 0.5048186264111258 1101 84-85 0.565064761582093 1101 86-87 0.68927694006317 1101 88-89 0.9064946999801293 1101 90-91 0.9498350235790731 1101 92-93 0.9270964617160367 1101 94-95 1.0292073321893902 1101 96-97 1.1291346891328935 1101 98-99 1.3911552522469428 1101 100-101 2.1819986470915786 1102 1 0.14053438649860794 1102 2 0.11652877661088468 1102 3 0.10267861694140379 1102 4 0.03886759020917907 1102 5 0.015384199075775484 1102 6 0.006054795237069754 1102 7 -0.0042733180325669196 1102 8 0.07032919899275214 1102 9 0.08154200353550323 1102 10-11 0.05171248803983275 1102 12-13 0.05338866189914171 1102 14-15 0.061424020170896654 1102 16-17 0.08956747542001864 1102 18-19 0.18750695712778764 1102 20-21 0.11713165919252333 1102 22-23 0.1439847826177072 1102 24-25 -0.051849083731120515 1102 26-27 -0.015615473927731216 1102 28-29 -0.022361377781756175 1102 30-31 0.09459642850071504 1102 32-33 0.11887452500835138 1102 34-35 0.08436341075300646 1102 36-37 0.04491205413000898 1102 38-39 -0.023431682389635 1102 40-41 -0.0016323920504532907 1102 42-43 0.033637590100099146 1102 44-45 0.06256247021933348 1102 46-47 0.08816583909458586 1102 48-49 0.1312690312871041 1102 50-51 0.11708394886032636 1102 52-53 0.15942725198131313 1102 54-55 0.1614208155307324 1102 56-57 0.2003493861116752 1102 58-59 0.2233726847876767 1102 60-61 0.1817722715208916 1102 62-63 0.1818102682129492 1102 64-65 0.3366895559577543 1102 66-67 0.24347009581114776 1102 68-69 0.18246446652069892 1102 70-71 0.20003693506054532 1102 72-73 0.29417202641673157 1102 74-75 0.39886728610332867 1102 76-77 0.33108810025366253 1102 78-79 0.16657267980745516 1102 80-81 0.19848076826752248 1102 82-83 0.3058442589393202 1102 84-85 0.34752480733433444 1102 86-87 0.4726310333891526 1102 88-89 0.6172764157557644 1102 90-91 0.8214345769134432 1102 92-93 0.7430168603700302 1102 94-95 0.777737713711538 1102 96-97 0.9918503809688168 1102 98-99 1.2367546731937473 1102 100-101 1.9275909135865383 1103 1 0.1684410644828347 1103 2 0.1684140136451049 1103 3 0.12015113961292911 1103 4 0.11797478247662241 1103 5 0.061200984292490546 1103 6 -0.01552025606295615 1103 7 0.08559015003658743 1103 8 0.04207526194529265 1103 9 0.13174269604254363 1103 10-11 0.14215557361431053 1103 12-13 0.16495712169163568 1103 14-15 0.1058059562502649 1103 16-17 0.18355579343788975 1103 18-19 0.1122682446943557 1103 20-21 0.17456775346946074 1103 22-23 0.10518564710726253 1103 24-25 0.0915736770460498 1103 26-27 0.06825820872428778 1103 28-29 0.046771435027054054 1103 30-31 0.033086541586150986 1103 32-33 0.05307699420579581 1103 34-35 0.12980766830515478 1103 36-37 0.19740719631725057 1103 38-39 0.20899220667609342 1103 40-41 0.2171557275633802 1103 42-43 0.2404914749980236 1103 44-45 0.332507415008088 1103 46-47 0.4282410346380203 1103 48-49 0.3513271256652928 1103 50-51 0.1067594063997106 1103 52-53 0.042540813496621865 1103 54-55 0.09470100245077617 1103 56-57 0.22253279090790556 1103 58-59 0.10849841580007791 1103 60-61 0.23680247421755496 1103 62-63 0.19622849745871918 1103 64-65 0.20725125399389555 1103 66-67 0.1655120831246677 1103 68-69 0.35764288104989106 1103 70-71 0.2609673344968897 1103 72-73 0.15813593227849054 1103 74-75 0.14809795904735878 1103 76-77 0.056620509250290496 1103 78-79 0.2969665943307547 1103 80-81 0.4155856436252705 1103 82-83 0.2853905614191312 1103 84-85 0.3797487320448276 1103 86-87 0.4171341634016983 1103 88-89 0.7171234518658522 1103 90-91 0.8614265377707753 1103 92-93 0.8962279751565738 1103 94-95 0.8523869699218061 1103 96-97 0.9564849579854346 1103 98-99 1.273107782089891 1103 100-101 1.9133561959326038 1104 1 0.003826977621635308 1104 2 0.04358699409831246 1104 3 0.09919653760344005 1104 4 0.015549252645826073 1104 5 0.015681160663085336 1104 6 -0.005335858205135935 1104 7 -0.05840687247657428 1104 8 7.121794475111187E-4 1104 9 -0.03297724582490957 1104 10-11 -0.029799631742662314 1104 12-13 0.07381517976721597 1104 14-15 0.06384695088092229 1104 16-17 0.04687383814742674 1104 18-19 -0.04367901780313588 1104 20-21 0.0942657462709704 1104 22-23 -0.027620009006199098 1104 24-25 -0.09898330959277502 1104 26-27 9.519225173590939E-4 1104 28-29 -0.1529970212578604 1104 30-31 -0.020336239661652655 1104 32-33 0.09592473875603957 1104 34-35 0.05301107699266083 1104 36-37 0.1706982588548911 1104 38-39 0.14611441979773332 1104 40-41 0.09018981165966977 1104 42-43 0.0956177403083629 1104 44-45 0.25349355642551075 1104 46-47 0.18577250110239163 1104 48-49 0.37379691313552144 1104 50-51 0.36619791289064807 1104 52-53 0.385295459480659 1104 54-55 0.38809517245374536 1104 56-57 0.35970186158605344 1104 58-59 0.3160155627480208 1104 60-61 0.353342453543263 1104 62-63 0.5296180581231056 1104 64-65 0.520960901440283 1104 66-67 0.5382880859423764 1104 68-69 0.5510489217496257 1104 70-71 0.5212961866639034 1104 72-73 0.5021405617633903 1104 74-75 0.36973863001700025 1104 76-77 0.5124129316643895 1104 78-79 0.4592734501921889 1104 80-81 0.5145500881530793 1104 82-83 0.4030197878908375 1104 84-85 0.5299670590807004 1104 86-87 0.48697945010116683 1104 88-89 0.6840797565865522 1104 90-91 0.815457459937214 1104 92-93 0.8319712943944317 1104 94-95 0.8136610577972654 1104 96-97 0.9489992549240966 1104 98-99 1.277831529179963 1104 100-101 1.9443229534661093 1105 1 0.007718785604666323 1105 2 0.026966193773013458 1105 3 0.020289225289388924 1105 4 0.05089422827587953 1105 5 0.056196346246579765 1105 6 -0.04992018160085365 1105 7 -0.07060378784737509 1105 8 0.0211411531644643 1105 9 0.04901990972415149 1105 10-11 0.029866344297076353 1105 12-13 -3.191869023950744E-4 1105 14-15 0.007502300428463116 1105 16-17 0.1467609664426348 1105 18-19 0.13534393070638373 1105 20-21 0.10395139306162093 1105 22-23 0.15720522050557406 1105 24-25 0.0038773958580051726 1105 26-27 -0.007084040706864414 1105 28-29 -0.014653465578703617 1105 30-31 -0.037377050105341425 1105 32-33 0.03184931260236823 1105 34-35 0.003109089560069833 1105 36-37 0.037197592183972006 1105 38-39 -0.08132245905029123 1105 40-41 -0.07494052139058738 1105 42-43 -0.13159399876886368 1105 44-45 -0.1504511093191283 1105 46-47 -0.09816076899176096 1105 48-49 0.07168379245159429 1105 50-51 -0.026820934549441233 1105 52-53 -0.035150722315925975 1105 54-55 0.07253731004541919 1105 56-57 0.14561204870795308 1105 58-59 0.06842506570221474 1105 60-61 0.07339013750808476 1105 62-63 0.1745410141299999 1105 64-65 0.16519850871410569 1105 66-67 0.2304841387221046 1105 68-69 0.2217938828748487 1105 70-71 0.27308752850655793 1105 72-73 0.215222254125095 1105 74-75 0.17180363456572323 1105 76-77 0.12177084633558621 1105 78-79 0.04287694104404238 1105 80-81 0.16792578059894225 1105 82-83 0.21193814854436255 1105 84-85 0.3106363055304051 1105 86-87 0.47989426170856575 1105 88-89 0.6626067939615048 1105 90-91 0.6781376406474173 1105 92-93 0.5750897466085938 1105 94-95 0.6971262716249349 1105 96-97 0.859414184853442 1105 98-99 0.9125623067183355 1105 100-101 1.4901569841188866 1106 1 -0.19460684441531484 1106 2 9.5907104073234E-4 1106 3 -0.09637112404138293 1106 4 -0.04137576954502009 1106 5 -0.005705744766025589 1106 6 -0.08026017831686261 1106 7 -0.0983469950488356 1106 8 -0.14560448020088756 1106 9 -0.11558249710409996 1106 10-11 -0.32240970934247315 1106 12-13 -0.3473582617988953 1106 14-15 -0.28464577536728797 1106 16-17 -0.22641547828349218 1106 18-19 -0.16214355674845393 1106 20-21 -0.03994383532925383 1106 22-23 -0.09058820376159105 1106 24-25 -0.003756302116052268 1106 26-27 -0.03333189997400865 1106 28-29 -0.02908355875813129 1106 30-31 -0.05318816534275328 1106 32-33 0.012322366607151025 1106 34-35 -0.4333050543923136 1106 36-37 -0.006237608830076624 1106 38-39 0.025400075117026688 1106 40-41 0.05410979887531653 1106 42-43 0.046369957121299876 1106 44-45 0.07468330031431947 1106 46-47 0.024775514665321907 1106 48-49 0.06218218038002021 1106 50-51 0.15021968643188188 1106 52-53 -0.05781928009115944 1106 54-55 0.010095384137791541 1106 56-57 -0.03881381287947505 1106 58-59 -0.01672435783449089 1106 60-61 0.031389189140412554 1106 62-63 0.0815054069905301 1106 64-65 0.036001924218844295 1106 66-67 -0.08564748220398144 1106 68-69 -0.0030040231201269307 1106 70-71 0.12603543849143506 1106 72-73 0.2618837083502399 1106 74-75 0.17085133454393286 1106 76-77 0.003594672078396144 1106 78-79 0.13712251302637668 1106 80-81 0.04800762334792097 1106 82-83 0.13640907486018605 1106 84-85 0.21439281213061534 1106 86-87 0.32002884973642054 1106 88-89 0.40702026768803634 1106 90-91 0.34284569340919546 1106 92-93 0.4632778886366289 1106 94-95 0.05382650649171694 1106 96-97 0.5179282112573489 1106 98-99 0.630804554042804 1106 100-101 1.2155492024398775 1107 1 -0.03330023324339493 1107 2 0.03939697438512013 1107 3 -0.012259421131581405 1107 4 -0.00822617064293496 1107 5 -0.04532988906324675 1107 6 -0.0880629010446583 1107 7 -0.10099742718895044 1107 8 -0.11891043376829202 1107 9 -0.013021515517024795 1107 10-11 -0.11278718863405857 1107 12-13 -0.025380179226637267 1107 14-15 -0.10998172550371521 1107 16-17 -0.0954170834920447 1107 18-19 -0.13131880426916354 1107 20-21 -0.09583327607949599 1107 22-23 -0.17950496369100932 1107 24-25 -0.296489006300213 1107 26-27 -0.2563324350435323 1107 28-29 -0.1339031668094961 1107 30-31 -0.18535237858633735 1107 32-33 -0.22158628558949545 1107 34-35 -0.16411867660873014 1107 36-37 -0.058478569831272864 1107 38-39 -0.14518509079537267 1107 40-41 -0.14243410133217083 1107 42-43 -0.11890934099820072 1107 44-45 -0.03296972548338317 1107 46-47 -0.07224147277075588 1107 48-49 -0.04834612929045079 1107 50-51 -0.056099137395456466 1107 52-53 -0.06979115422320348 1107 54-55 -0.06152157756594079 1107 56-57 -0.1305420874232297 1107 58-59 -0.11735720007475692 1107 60-61 0.09728973973103905 1107 62-63 0.020998694114553018 1107 64-65 0.007854306299329039 1107 66-67 0.04169870573203127 1107 68-69 0.1279687924891988 1107 70-71 0.047331664821747665 1107 72-73 0.15223325987211922 1107 74-75 0.06623635994952437 1107 76-77 0.06898001403274989 1107 78-79 0.15661088853898164 1107 80-81 0.19585580656226043 1107 82-83 0.24085929093024205 1107 84-85 0.26306361367292297 1107 86-87 0.5157491615471628 1107 88-89 0.5645172655804345 1107 90-91 0.5555395539970363 1107 92-93 0.5953587780773795 1107 94-95 0.4316613027568508 1107 96-97 0.644524178630359 1107 98-99 0.752828280904108 1107 100-101 1.3146241652709278 1108 1 -0.02213535377675413 1108 2 -0.0022613783711875612 1108 3 -0.0482063187237074 1108 4 -0.028106675735131148 1108 5 -0.0019532267954858185 1108 6 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-0.2955504764977803 2316 9 -0.17575233732171114 2316 10-11 -0.20199395226198646 2316 12-13 0.06866597986338974 2316 14-15 0.013530385283722524 2316 16-17 -0.07555156190065304 2316 18-19 0.1384931297671912 2316 20-21 0.23761519729012548 2316 22-23 0.009826896130974205 2316 24-25 -0.02324799583219317 2316 26-27 -0.047715029102867845 2316 28-29 0.07813593629295923 2316 30-31 -0.12519024548539193 2316 32-33 -0.1862794660899496 2316 34-35 -0.08725953984275492 2316 36-37 -0.013016645106851854 2316 38-39 -0.005282743133363965 2316 40-41 -0.09379891486504732 2316 42-43 -0.19519517877373005 2316 44-45 -0.28649789496764555 2316 46-47 -0.30341484695947685 2316 48-49 -0.4753068188656471 2316 50-51 -0.7232352076179964 2316 52-53 -0.5881370733112661 2316 54-55 -0.31405934361497145 2316 56-57 -0.32614351548765086 2316 58-59 -0.7599735377782508 2316 60-61 -0.30228226261627356 2316 62-63 -0.24540665269355344 2316 64-65 -0.3336727106132571 2316 66-67 -0.2777031517356079 2316 68-69 -0.08272341816435613 2316 70-71 -0.12945315215478814 2316 72-73 -0.13555211582055904 2316 74-75 -0.05021168120572028 2316 76-77 0.11958441137480591 2316 78-79 -0.004855794057540663 2316 80-81 -0.12222762135457188 2316 82-83 -0.13518847754340868 2316 84-85 -0.04264267030246671 2316 86-87 -0.08659867615106975 2316 88-89 0.21109468993413216 2316 90-91 0.4102735313180794 2316 92-93 0.5356685668545644 2316 94-95 0.8616401137105001 2316 96-97 0.8972067189264443 2316 98-99 1.3277273430340486 2316 100-101 1.9065087253785968 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 11.0 4 18.0 5 52.0 6 93.0 7 235.0 8 617.0 9 1139.0 10 1994.0 11 3330.0 12 5100.0 13 7114.0 14 9555.0 15 13320.0 16 17892.0 17 25368.0 18 22964.0 19 14569.0 20 12774.0 21 13034.0 22 13881.0 23 15160.0 24 17520.0 25 21375.0 26 25422.0 27 31739.0 28 39430.0 29 48931.0 30 60679.0 31 76997.0 32 97416.0 33 127348.0 34 172554.0 35 244242.0 36 366947.0 37 530063.0 38 381081.0 39 28329.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.900416545102836 20.293068941197433 15.796187921744767 22.010326591954968 2 15.38560822382542 21.230069341964082 39.88329049410183 23.50103194010867 3 19.137066888425892 25.312002078175844 30.37318532320985 25.177745710188415 4 12.953554321956329 17.790908879259533 37.66942871811922 31.58610808066492 5 13.419045417302147 37.930231665246616 34.24939365446841 14.40132926298282 6 30.909107989581347 37.321391446558245 17.54604519624017 14.223455367620238 7 27.65730194122373 33.49098007148631 20.944891988362542 17.90682599892742 8 26.157445510172156 35.25199720132451 19.765135087556637 18.825422200946697 9 26.04810437932264 17.266994540295492 20.78343308277863 35.901467997603234 10-11 24.401329738459445 27.524046267186918 27.296929585046936 20.777694409306697 12-13 25.612953862800538 24.740974415332623 28.119422481854272 21.52664924001257 14-15 22.598834865484267 26.050922660244797 26.811838086901897 24.538404387369038 16-17 21.390425441969757 29.46290100767839 27.344513603515253 21.802159946836603 18-19 21.321969806817055 27.725471663819114 30.130670207625617 20.821888321738218 20-21 22.26856965007499 26.62874505370266 30.373737489257834 20.728947806964516 22-23 21.89375105814147 26.640514957755013 30.211901954781695 21.253832029321828 24-25 21.75749367213727 26.851499755544562 30.315128993640073 21.075877578678096 26-27 21.527674529172746 27.0137086009401 30.28460398555046 21.174012884336694 28-29 21.342585567405298 27.307637872127767 30.273508941155374 21.076267619311565 30-31 21.59557978103129 26.837227539981907 30.295246283638207 21.2719463953486 32-33 21.39320135449281 27.006900512254134 30.202183436524134 21.39771469672893 34-35 21.86589802479352 27.02574152442857 30.040138038582676 21.068222412195233 36-37 21.875566719764734 26.789445737858923 30.00161336437528 21.333374178001062 38-39 21.597357676215676 27.116290306802743 30.079877890756425 21.20647412622516 40-41 21.80128178683912 27.092420417260787 29.90861826221531 21.197679533684788 42-43 21.592184681446234 27.09829073096128 29.712560619330215 21.596963968262266 44-45 21.785474906884406 27.2418301507862 29.545693115348154 21.42700182698124 46-47 21.99864314069226 27.475318598489316 29.044958115852587 21.481080144965837 48-49 22.07112197397297 27.5814125795807 28.797439530515245 21.55002591593109 50-51 22.04334761126185 27.78898509619528 28.619418118932273 21.548249173610596 52-53 22.22560098321264 27.88642570632159 28.29107374687053 21.596899563595237 54-55 22.026294969450245 27.847801393701182 28.42168916321972 21.704214473628856 56-57 22.140925482610186 28.062766792155045 28.269297770167967 21.527009955066802 58-59 22.282288819879582 27.72496110568104 28.38658318564588 21.606166888793492 60-61 22.104287830622134 27.72835121171784 28.68338084246994 21.483980115190086 62-63 21.884278117764115 27.773995109261485 28.798093044931978 21.543633728042426 64-65 21.970337797517544 27.753531939087555 28.581024146949254 21.69510611644565 66-67 21.909560956761446 27.8613822401739 28.365262277799992 21.863794525264662 68-69 21.900227953997486 27.767337431141026 28.55721171538957 21.77522289947192 70-71 22.078412744184643 27.631896568273262 28.41445965998462 21.875231027557476 72-73 22.084698057466067 27.563669767189193 28.373661365430745 21.977970809913995 74-75 21.913767955819157 28.05482250752665 27.99788506396885 22.033524472685347 76-77 21.986573546308236 27.7899857901459 28.234722773057726 21.98871789048814 78-79 21.955470344536884 27.81261372682526 28.114047251543006 22.11786867709485 80-81 21.96784627380375 28.058784438678085 28.05423026008648 21.919139027431676 82-83 21.981287767408414 27.93419411046438 28.098431089598602 21.986087032528605 84-85 21.926386442178835 27.927552966365628 28.03070621684688 22.115354374608657 86-87 21.890813261931438 27.815574964026073 28.10649099109954 22.187120782942944 88-89 22.01481762250638 27.620010774818947 28.013406031366237 22.35176557130843 90-91 21.990908249792128 27.562459572307436 28.16265315546885 22.283979022431584 92-93 21.91005854949443 27.492693311462112 28.231786479476025 22.365461659567437 94-95 21.754065370230215 27.525945543460544 28.200311154551667 22.519677931757574 96-97 21.679483037420646 27.665287070503176 28.170453714637212 22.484776177438967 98-99 21.623385123871074 27.666803564853286 27.947223604705567 22.762587706570073 100-101 21.63916184242051 27.46585990115461 28.275450889912822 22.619527366512056 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 354.0 1 265.0 2 217.5 3 300.0 4 298.5 5 310.0 6 548.0 7 1161.0 8 2110.0 9 2807.5 10 2813.0 11 2698.0 12 2843.0 13 3092.5 14 3356.0 15 3558.0 16 3754.0 17 3998.5 18 4214.0 19 4466.5 20 4942.5 21 5640.5 22 6675.0 23 7955.5 24 9490.5 25 11858.0 26 15300.5 27 19116.5 28 22144.0 29 25860.0 30 30735.0 31 35576.5 32 40972.0 33 46739.0 34 53128.0 35 59058.0 36 65395.5 37 71268.5 38 74822.0 39 77438.0 40 79575.0 41 83222.5 42 86470.5 43 89985.0 44 94726.0 45 96522.5 46 96294.0 47 97865.5 48 101151.5 49 99057.5 50 92282.0 51 85420.5 52 79384.0 53 76003.5 54 72833.5 55 65772.0 56 57207.0 57 50116.5 58 44415.0 59 38948.0 60 31786.0 61 25394.0 62 20265.5 63 15096.5 64 11040.0 65 8282.0 66 6019.5 67 4602.5 68 3476.0 69 2365.5 70 1599.0 71 1084.5 72 802.5 73 639.0 74 481.0 75 373.5 76 271.5 77 147.5 78 84.5 79 53.0 80 30.5 81 19.0 82 14.0 83 8.5 84 4.5 85 2.5 86 0.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041947456624001705 2 0.0 3 0.0 4 0.0 5 0.0011028055782356825 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005534450216701296 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.0422325522883014E-4 22-23 9.190046485297355E-4 24-25 8.168930209153204E-5 26-27 0.0023281451096086635 28-29 0.004064042779053719 30-31 2.4506790627459613E-4 32-33 1.838009297059471E-4 34-35 0.0 36-37 4.084465104576602E-5 38-39 6.126697656864903E-5 40-41 0.0 42-43 0.009680182297846547 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.042232552288301E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.633786041830641E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.8802418493477723E-4 84-85 8.168930209153204E-5 86-87 0.0 88-89 0.0 90-91 0.010517497644284751 92-93 0.0012457618568958637 94-95 0.0 96-97 0.0 98-99 3.8802418493477723E-4 100-101 0.013744225076900266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2448301.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.17849125976999 #Duplication Level Percentage of deduplicated Percentage of total 1 76.81857962230339 43.155519038995166 2 13.221517427992477 14.85529802538744 3 4.2883140742762365 7.227330442226286 4 1.8662829047934049 4.193798314207778 5 0.981727066984372 2.757597272603059 6 0.6157408044603262 2.075483364099491 7 0.39597253561929247 1.5571597741978171 8 0.2819162392358149 1.2670103185517163 9 0.21988483333167425 1.1117518368731498 >10 1.1776755541680124 12.206281465510015 >50 0.08819256322835885 3.3941121319630523 >100 0.04230966384058028 4.237837381898923 >500 0.0013787494443885159 0.5282911201761785 >1k 2.9026304092373084E-4 0.3167545338195098 >5k 1.4513152046186542E-4 0.6339167311317088 >10k+ 7.256576023093271E-5 0.48185824835863655 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11820 0.4827837753609544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9367 0.3825918463456903 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6183 0.25254247741597136 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2575 0.1051749764428475 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2540 0.1037454136562457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.084465104576602E-5 0.0 0.0 0.0 0.0 7 4.084465104576602E-5 0.0 0.0 0.0 0.0 8 4.084465104576602E-5 0.0 0.0 0.0 0.0 9 4.084465104576602E-5 0.0 0.0 0.0 0.0 10-11 1.2253395313729806E-4 0.0 0.0 0.0 0.0 12-13 1.2253395313729806E-4 2.042232552288301E-5 0.0 0.0 0.0 14-15 1.2253395313729806E-4 4.084465104576602E-5 0.0 0.0 0.0 16-17 1.2253395313729806E-4 4.084465104576602E-5 0.0 0.0 0.0 18-19 1.2253395313729806E-4 4.084465104576602E-5 0.0 2.042232552288301E-5 0.0 20-21 1.2253395313729806E-4 8.168930209153204E-5 0.0 4.084465104576602E-5 0.0 22-23 1.633786041830641E-4 8.168930209153204E-5 0.0 1.4295627866018108E-4 0.0 24-25 2.042232552288301E-4 1.0211162761441505E-4 0.0 3.267572083661282E-4 0.0 26-27 2.042232552288301E-4 1.2253395313729806E-4 0.0 5.105581380720753E-4 0.0 28-29 2.042232552288301E-4 1.2253395313729806E-4 0.0 0.0011028055782356825 0.0 30-31 2.042232552288301E-4 1.2253395313729806E-4 0.0 0.0027161692945434405 0.0 32-33 2.042232552288301E-4 1.633786041830641E-4 0.0 0.0042886883598054325 0.0 34-35 2.042232552288301E-4 1.633786041830641E-4 0.0 0.0063717655631395 0.0 36-37 2.042232552288301E-4 1.633786041830641E-4 0.0 0.008352731138859152 0.0 38-39 2.042232552288301E-4 2.042232552288301E-4 0.0 0.010354119040101688 0.0 40-41 2.042232552288301E-4 2.042232552288301E-4 0.0 0.01329493391539684 0.0 42-43 2.042232552288301E-4 2.042232552288301E-4 0.0 0.019156141340464266 0.0 44-45 2.4506790627459613E-4 2.042232552288301E-4 0.0 0.027508872479323418 0.0 46-47 3.0633488284324514E-4 2.042232552288301E-4 0.0 0.04715514963233687 0.0 48-49 3.267572083661282E-4 2.042232552288301E-4 0.0 0.08336393278440846 0.0 50-51 3.267572083661282E-4 2.042232552288301E-4 0.0 0.11644810013147894 0.0 52-53 3.267572083661282E-4 2.042232552288301E-4 0.0 0.15022662654632746 0.0 54-55 3.267572083661282E-4 2.042232552288301E-4 0.0 0.19409378176948017 0.0 56-57 3.267572083661282E-4 2.042232552288301E-4 0.0 0.23003707468975426 0.0 58-59 3.267572083661282E-4 2.042232552288301E-4 0.0 0.27788658338986916 0.0 60-61 3.267572083661282E-4 2.042232552288301E-4 0.0 0.35426608084545164 0.0 62-63 3.267572083661282E-4 2.042232552288301E-4 0.0 0.4119591504475961 0.0 64-65 3.267572083661282E-4 2.4506790627459613E-4 0.0 0.4824570181525883 0.0 66-67 3.676018594118942E-4 2.4506790627459613E-4 0.0 0.6038064764095591 0.0 68-69 3.676018594118942E-4 2.4506790627459613E-4 0.0 0.7463338862337596 0.0 70-71 3.676018594118942E-4 2.4506790627459613E-4 0.0 0.8789973128304076 0.0 72-73 3.676018594118942E-4 2.4506790627459613E-4 0.0 1.0207078296336929 4.084465104576602E-5 74-75 3.676018594118942E-4 2.4506790627459613E-4 0.0 1.157374032032826 4.084465104576602E-5 76-77 3.676018594118942E-4 2.4506790627459613E-4 0.0 1.2812558586546343 4.084465104576602E-5 78-79 3.676018594118942E-4 2.4506790627459613E-4 0.0 1.4325648684536745 4.084465104576602E-5 80-81 4.084465104576602E-4 2.4506790627459613E-4 0.0 1.607951800044194 4.084465104576602E-5 82-83 4.4929116150342624E-4 2.4506790627459613E-4 0.0 1.8248777417482573 4.084465104576602E-5 84-85 4.901358125491923E-4 2.4506790627459613E-4 0.0 2.074887850799391 4.084465104576602E-5 86-87 5.309804635949583E-4 2.4506790627459613E-4 0.0 2.4571529399367154 4.084465104576602E-5 88-89 5.309804635949583E-4 2.4506790627459613E-4 0.0 2.956968934783754 4.084465104576602E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8775 0.0 34.551582 1 GTATCAA 13440 0.0 32.777428 1 ATCAACG 16370 0.0 26.635853 3 TCAACGC 16485 0.0 26.450039 4 TATCAAC 16805 0.0 26.144228 2 CAACGCA 16865 0.0 25.85407 5 AACGCAG 17240 0.0 25.236597 6 GTACATG 10090 0.0 22.936768 1 TACATGG 10190 0.0 22.280571 2 ACGCAGA 19500 0.0 22.240484 7 CGCAGAG 19630 0.0 22.0448 8 ACATGGG 10215 0.0 21.57507 3 AGTACTT 11120 0.0 21.465418 12-13 GAGTACT 11095 0.0 20.95721 12-13 AGAGTAC 16210 0.0 20.937618 10-11 GTACTTT 11170 0.0 20.665964 14-15 GCAGAGT 20835 0.0 20.31385 9 TACTTTT 12570 0.0 18.742132 14-15 CATGGGA 5180 0.0 17.788713 4 ACTTTTT 13320 0.0 17.473057 16-17 >>END_MODULE