FastQCFastQC Report
Thu 26 May 2016
SRR937494_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937494_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401256
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99690.4151577341191443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76110.3169591247247274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48830.20335191249912543No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35400.14742284870917555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119800.035.6911161
GGTATCA74750.034.2316931
ATCAACG144650.029.2459453
TCAACGC144900.029.1007234
CAACGCA146650.028.785275
TATCAAC148100.028.6609442
AACGCAG150350.028.11786
GTATAGG7250.025.5849111
ACGCAGA170400.024.5584587
CGCAGAG170450.024.3561828
GTGTACG3500.023.1013931
GAGTACT86500.022.73440412-13
GCAGAGT179000.022.5829339
GTACATG104700.022.3499071
TACATGG103500.022.2279342
GTACTTT88850.021.89183414-15
AGTACTT89500.021.1497212-13
ACATGGG104650.020.9844133
AGAGTAC143000.020.96028310-11
TAGCCCT18000.019.2796384
Produced by FastQC (version 0.11.5)