##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937494_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2401256 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58114336830392 33.0 31.0 34.0 30.0 34.0 2 31.69380357612849 33.0 31.0 34.0 30.0 34.0 3 31.72534207098285 33.0 31.0 34.0 30.0 34.0 4 35.346440779325484 37.0 35.0 37.0 33.0 37.0 5 35.34580694436578 37.0 35.0 37.0 33.0 37.0 6 35.37684320205759 37.0 35.0 37.0 33.0 37.0 7 35.31891809952791 37.0 35.0 37.0 33.0 37.0 8 35.30966252661107 37.0 35.0 37.0 33.0 37.0 9 36.90409102569655 39.0 37.0 39.0 33.0 39.0 10-11 36.77969966550839 39.0 37.0 39.0 33.0 39.0 12-13 36.644297192802426 39.0 37.0 39.0 32.0 39.0 14-15 37.78954680383932 40.0 38.0 41.0 32.5 41.0 16-17 37.75109380257665 40.0 38.0 41.0 32.0 41.0 18-19 37.72764586533047 40.0 37.5 41.0 32.0 41.0 20-21 37.66917125870795 40.0 37.0 41.0 32.0 41.0 22-23 37.51752832684228 40.0 37.0 41.0 32.0 41.0 24-25 37.35980815873026 40.0 37.0 41.0 32.0 41.0 26-27 37.20092110129032 39.5 37.0 41.0 31.0 41.0 28-29 37.25157334328368 40.0 37.0 41.0 31.0 41.0 30-31 37.098638795696914 39.0 36.5 41.0 31.0 41.0 32-33 36.898709883494305 39.0 36.0 41.0 30.5 41.0 34-35 36.77767593292843 39.0 36.0 41.0 30.0 41.0 36-37 36.78860271457937 39.0 36.0 41.0 30.0 41.0 38-39 36.69689404211796 39.0 36.0 41.0 30.0 41.0 40-41 36.52893131761045 39.0 35.5 41.0 30.0 41.0 42-43 36.288704328068306 39.0 35.0 41.0 30.0 41.0 44-45 36.06346470347185 39.0 35.0 41.0 29.5 41.0 46-47 35.72000652991601 39.0 35.0 41.0 27.5 41.0 48-49 35.406002108896345 39.0 35.0 40.5 26.5 41.0 50-51 34.521708639145515 38.0 34.0 39.5 25.0 40.5 52-53 34.616932347071696 38.0 34.0 39.5 25.5 40.5 54-55 34.93936194225022 39.0 35.0 40.0 25.5 41.0 56-57 34.7443664898703 38.0 34.0 40.0 24.5 41.0 58-59 34.508013931042754 38.0 34.0 40.0 23.5 41.0 60-61 34.22440776826794 38.0 34.0 40.0 23.0 41.0 62-63 33.90835983335388 38.0 33.5 40.0 22.0 41.0 64-65 33.54656209083913 37.0 33.0 40.0 21.5 41.0 66-67 33.1772291250912 36.5 33.0 39.5 20.5 41.0 68-69 32.69635411634578 36.0 32.0 39.0 18.5 40.5 70-71 32.25478374650599 35.5 31.5 39.0 17.5 40.0 72-73 31.85592873063097 35.0 31.0 38.0 14.0 40.0 74-75 31.392957893702295 35.0 31.0 37.0 10.0 39.5 76-77 30.95420542416136 35.0 31.0 37.0 7.5 39.0 78-79 30.472183932075545 34.5 30.0 36.0 6.5 39.0 80-81 29.980867096219647 34.0 30.0 36.0 2.0 37.5 82-83 29.493403660417716 34.0 29.0 35.0 2.0 37.0 84-85 29.04411878616857 34.0 29.0 35.0 2.0 36.5 86-87 28.61247717860986 34.0 29.0 35.0 2.0 36.0 88-89 28.205224265967477 34.0 28.0 35.0 2.0 36.0 90-91 27.779307579033638 34.0 27.0 35.0 2.0 35.5 92-93 27.31609124558148 33.0 26.0 35.0 2.0 35.0 94-95 26.780582120357014 33.0 25.0 35.0 2.0 35.0 96-97 26.14744179712617 33.0 24.5 35.0 2.0 35.0 98-99 25.38479716448392 32.0 21.5 34.5 2.0 35.0 100-101 23.506125127849756 30.5 8.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.00957999142728383 1101 2 0.008274070727289029 1101 3 -0.0031815063408089372 1101 4 -0.040306882403271516 1101 5 0.038184684463253404 1101 6 0.05695279689174271 1101 7 -0.02566990393503943 1101 8 0.09835042464173682 1101 9 0.06512306673328538 1101 10-11 0.08704965114596774 1101 12-13 0.012886500127095246 1101 14-15 -0.0020558513703221593 1101 16-17 0.15667944066227335 1101 18-19 0.20998734276839315 1101 20-21 0.15346976607135332 1101 22-23 0.13440171229576947 1101 24-25 0.2634299845025936 1101 26-27 0.1881238077044145 1101 28-29 0.1148806759858445 1101 30-31 0.16377518162467197 1101 32-33 0.13258239322018994 1101 34-35 0.14848856363486362 1101 36-37 0.25625076815882153 1101 38-39 0.28002548245132175 1101 40-41 0.14732048955828247 1101 42-43 0.26268575010841033 1101 44-45 0.32911645929321054 1101 46-47 0.44360319797092984 1101 48-49 0.5321964797406054 1101 50-51 0.32925311924221745 1101 52-53 0.33759766853133755 1101 54-55 0.46347197740598745 1101 56-57 0.35685000516266996 1101 58-59 0.4722385772039175 1101 60-61 0.4425668771747624 1101 62-63 0.374950265583216 1101 64-65 0.3504034216700518 1101 66-67 0.4157557788906914 1101 68-69 0.5330635150808192 1101 70-71 0.4847035967155904 1101 72-73 0.3079765243451753 1101 74-75 0.39181572810628396 1101 76-77 0.38972679702881763 1101 78-79 0.28787012573194914 1101 80-81 0.4119610149720039 1101 82-83 0.500250242385853 1101 84-85 0.49981309954397446 1101 86-87 0.5061004194290639 1101 88-89 0.4389600012505319 1101 90-91 0.587265084009271 1101 92-93 0.6023627961846394 1101 94-95 0.6293848369157722 1101 96-97 0.7508993827645121 1101 98-99 0.7763517792209491 1101 100-101 1.2406601844720484 1102 1 0.016779388000198736 1102 2 0.005497129941378631 1102 3 0.11980054480908464 1102 4 0.04013692923126655 1102 5 0.13230034528595525 1102 6 -0.07625834113526508 1102 7 0.11763502563869821 1102 8 0.010919327794724154 1102 9 0.08964959915526549 1102 10-11 0.15642006035569977 1102 12-13 0.22952422449598942 1102 14-15 0.3077224658266928 1102 16-17 0.3419705668084063 1102 18-19 0.3027955617201812 1102 20-21 0.23660911827929 1102 22-23 0.24229680604767623 1102 24-25 0.21565326342704338 1102 26-27 0.1707268766452259 1102 28-29 0.21074741190257384 1102 30-31 0.25452943904988956 1102 32-33 0.30781503841404856 1102 34-35 0.2788722522712419 1102 36-37 0.2999666056749106 1102 38-39 0.3768572920189328 1102 40-41 0.3592023104913622 1102 42-43 0.4375759857832904 1102 44-45 0.24242713272365535 1102 46-47 0.32335104811326687 1102 48-49 0.279026166542657 1102 50-51 0.2126382084082863 1102 52-53 0.38326372463446035 1102 54-55 0.37958585774874365 1102 56-57 0.30247197877628196 1102 58-59 0.23913942538504074 1102 60-61 0.30308858356770685 1102 62-63 0.09841714151988157 1102 64-65 0.2579704448952498 1102 66-67 0.3106386583982186 1102 68-69 0.35679682700477855 1102 70-71 0.2768736561728531 1102 72-73 0.18531916546861638 1102 74-75 0.23209909500739556 1102 76-77 0.20019658239326432 1102 78-79 0.11505024726316648 1102 80-81 0.18329827798216058 1102 82-83 0.17738548848789293 1102 84-85 0.13841449273896345 1102 86-87 0.10344012337182562 1102 88-89 0.26894048006714755 1102 90-91 0.296048740994145 1102 92-93 0.3129967696185183 1102 94-95 0.2817636806907977 1102 96-97 0.3150680251725113 1102 98-99 0.39575358388554704 1102 100-101 0.766049286573601 1103 1 -0.1473808496978819 1103 2 -0.12089726121791955 1103 3 -0.147698412655469 1103 4 -0.2694543968738259 1103 5 -0.3247080992511613 1103 6 -0.23595131444636053 1103 7 -0.20943440722201956 1103 8 -0.08774622683412758 1103 9 -0.1963595751566629 1103 10-11 -0.08350122821811112 1103 12-13 -0.1411661946032723 1103 14-15 -0.2115422860498697 1103 16-17 -0.2674018268529821 1103 18-19 -0.2723816693056733 1103 20-21 -0.4473488675422317 1103 22-23 -0.5643689049614977 1103 24-25 -0.4556561610933869 1103 26-27 -0.21067038148655115 1103 28-29 -0.38528183121669457 1103 30-31 -0.42280584034961066 1103 32-33 -0.3409326158812007 1103 34-35 -0.2873965133667937 1103 36-37 -0.24374820875377878 1103 38-39 -0.42248591064996077 1103 40-41 -0.24204873204408273 1103 42-43 -0.3468172585870164 1103 44-45 -0.13464099696775378 1103 46-47 -0.11934859742468973 1103 48-49 -0.12601449233974904 1103 50-51 -0.20771859934817627 1103 52-53 -0.15368112524043198 1103 54-55 -0.24391680071664013 1103 56-57 -0.19140964132380134 1103 58-59 -0.28507997619961145 1103 60-61 -0.19612821167833516 1103 62-63 -0.46456894063191356 1103 64-65 -0.3127968611931564 1103 66-67 -0.360752865788605 1103 68-69 -0.16198314588929463 1103 70-71 -0.12261810779712334 1103 72-73 -0.022339560553074733 1103 74-75 -0.15952934535957652 1103 76-77 -0.09468978124310112 1103 78-79 -0.06580619560589085 1103 80-81 -0.14301401351074716 1103 82-83 -0.1754593301109395 1103 84-85 -0.06484864320765737 1103 86-87 -0.06528120690340344 1103 88-89 -0.15263218465345219 1103 90-91 0.03380807168639066 1103 92-93 0.02601543100300674 1103 94-95 0.08510630996519453 1103 96-97 0.2891128020699263 1103 98-99 0.28941496837262193 1103 100-101 0.7114790718112971 1104 1 -0.01578662862467084 1104 2 -0.08264466951138161 1104 3 -0.08711293135691278 1104 4 -0.14247331697465881 1104 5 -0.08063831610561323 1104 6 -0.15649219652692636 1104 7 -0.0522953168274114 1104 8 -0.042224477078512734 1104 9 0.0065585063373987396 1104 10-11 -7.323788586433011E-4 1104 12-13 -0.009645206925128491 1104 14-15 -0.007150452819438158 1104 16-17 -0.022494080029915153 1104 18-19 0.021999215090069413 1104 20-21 0.024884823356572383 1104 22-23 -0.052317228391039805 1104 24-25 -0.139215706033454 1104 26-27 -0.04398064348686148 1104 28-29 -0.05334829273338215 1104 30-31 -0.14715887836987918 1104 32-33 -0.12546011176533511 1104 34-35 -0.03268369117200365 1104 36-37 0.01815684025069686 1104 38-39 0.00817404201763594 1104 40-41 0.014014767981642251 1104 42-43 0.09051923238381221 1104 44-45 -0.024098683982209934 1104 46-47 0.027449390948007135 1104 48-49 0.042438876235650014 1104 50-51 -0.07254832165615 1104 52-53 -0.14195654711624428 1104 54-55 0.021214054739452592 1104 56-57 -0.0335964982204473 1104 58-59 -0.09566714564449086 1104 60-61 -0.0809944622571308 1104 62-63 -0.3349684203612995 1104 64-65 -0.0893930437316115 1104 66-67 -0.012584986872106185 1104 68-69 0.013454887886489075 1104 70-71 -0.02081227356345039 1104 72-73 0.09896958887842544 1104 74-75 -0.02117691185646109 1104 76-77 -0.009348196893665062 1104 78-79 0.06416708344952227 1104 80-81 0.06673872932507763 1104 82-83 0.0024541692716795183 1104 84-85 0.15500009888217292 1104 86-87 0.10089911284758557 1104 88-89 -0.02415910496008422 1104 90-91 0.07820945500171561 1104 92-93 0.3710310584146441 1104 94-95 0.44548784711084366 1104 96-97 0.5006609287098414 1104 98-99 0.4225172668240269 1104 100-101 0.5316918907905688 1105 1 -0.061674060468003944 1105 2 -0.04161418362672009 1105 3 -0.019383415550663585 1105 4 -0.08361551441869608 1105 5 -0.10520715831311378 1105 6 -0.13220746673356132 1105 7 -0.013176445438581652 1105 8 0.006271578608831874 1105 9 -0.013785023911729866 1105 10-11 0.07388694491448433 1105 12-13 -7.592388997323951E-4 1105 14-15 0.012128638964604477 1105 16-17 -0.07675873189061377 1105 18-19 -0.07365917364846553 1105 20-21 -0.03833395729323996 1105 22-23 -0.041144925517372144 1105 24-25 0.06211128245033137 1105 26-27 0.07396066223087416 1105 28-29 0.08292260566808096 1105 30-31 -0.11678383687537774 1105 32-33 -0.08389458027031083 1105 34-35 -0.054834628595507695 1105 36-37 -0.14385949277230736 1105 38-39 0.11680910961639768 1105 40-41 0.07319084525759223 1105 42-43 0.009351314082351792 1105 44-45 -0.055257492335449854 1105 46-47 0.0630124332643689 1105 48-49 0.09431519787450071 1105 50-51 -0.04618034659774395 1105 52-53 -0.039438580364929976 1105 54-55 0.034576431767433746 1105 56-57 0.05406209434498521 1105 58-59 0.090521681298938 1105 60-61 0.13625543771367887 1105 62-63 0.057506008702191025 1105 64-65 0.17449323724343913 1105 66-67 0.12208134816622618 1105 68-69 0.14621296731278477 1105 70-71 0.2508908673042072 1105 72-73 0.3219686690358152 1105 74-75 0.17209702985756437 1105 76-77 0.11528597150733688 1105 78-79 0.2261433556390422 1105 80-81 0.20700317670349833 1105 82-83 0.3694315572660116 1105 84-85 0.39053641759141655 1105 86-87 0.16475100212960214 1105 88-89 0.1006647174889892 1105 90-91 0.30954725089889834 1105 92-93 0.3782193974187571 1105 94-95 0.44264557603352017 1105 96-97 0.5073208579471711 1105 98-99 0.5705111611925382 1105 100-101 0.8293813228246165 1106 1 0.01035407328382476 1106 2 -0.005308363542223304 1106 3 -0.16428485193479858 1106 4 -0.09511885155681199 1106 5 -0.09777189721567936 1106 6 0.05411066121035901 1106 7 -0.06733050129435725 1106 8 -0.1394834362963806 1106 9 -0.03634633763775241 1106 10-11 -0.42208128942014866 1106 12-13 -0.476371762957001 1106 14-15 -0.48654679884040775 1106 16-17 -0.42006781158685413 1106 18-19 -0.17699674976228152 1106 20-21 -0.2559695868380629 1106 22-23 -0.12898927182988018 1106 24-25 -0.165481580568013 1106 26-27 -0.22042896868225625 1106 28-29 -0.06572103318340794 1106 30-31 -0.13824388188447756 1106 32-33 0.0438380054226144 1106 34-35 -0.047723927065042915 1106 36-37 -0.1508386622677449 1106 38-39 -0.04321724059082044 1106 40-41 -0.06766161197806753 1106 42-43 -0.1417521884453592 1106 44-45 -0.012232413210362836 1106 46-47 0.13995376813713278 1106 48-49 0.20255576965997335 1106 50-51 0.09394060330620135 1106 52-53 -0.11687437582494908 1106 54-55 -0.12990286693861464 1106 56-57 -0.13832998412397046 1106 58-59 -0.07096203334443629 1106 60-61 -0.20950132919999476 1106 62-63 -0.11660635811231401 1106 64-65 0.01471654824158719 1106 66-67 0.09327920510948928 1106 68-69 -0.2342885801486787 1106 70-71 -0.15003524436706073 1106 72-73 0.04087840293200529 1106 74-75 -0.23440642554112046 1106 76-77 0.13060978209069063 1106 78-79 0.130249625464117 1106 80-81 0.07212038168936274 1106 82-83 0.04264190252555977 1106 84-85 -0.15057962931685864 1106 86-87 0.025145230312933364 1106 88-89 0.18371683886462975 1106 90-91 0.15863808990946637 1106 92-93 0.3019755809136093 1106 94-95 0.3208314939151329 1106 96-97 0.21858368812411655 1106 98-99 0.3609633282956004 1106 100-101 0.2154578488020853 1107 1 -0.11647004332676048 1107 2 -0.1128046990454159 1107 3 -0.01722071712745077 1107 4 -0.08448998837899069 1107 5 -0.08381954190274143 1107 6 -0.014931272998026657 1107 7 0.0036658312098794 1107 8 -0.023149031181354474 1107 9 0.02023275482198983 1107 10-11 -0.008004177426570891 1107 12-13 -0.01886488496063521 1107 14-15 -0.023741083840214117 1107 16-17 0.047612364696547615 1107 18-19 -0.07121914054135914 1107 20-21 -0.31825029951982486 1107 22-23 -0.130903576355216 1107 24-25 -0.14668646065810975 1107 26-27 -0.07508134554155532 1107 28-29 -0.09090425802364877 1107 30-31 -0.05446321926224584 1107 32-33 -0.07543657877177168 1107 34-35 0.04085290095021321 1107 36-37 -0.016130006488680237 1107 38-39 0.030722869743179615 1107 40-41 0.06215449649011617 1107 42-43 -0.0028505732062171774 1107 44-45 0.033850600733870806 1107 46-47 0.1777919526321483 1107 48-49 0.3248367407135717 1107 50-51 0.31959341893621485 1107 52-53 0.29170176935048886 1107 54-55 0.392408324390793 1107 56-57 0.37597790927365793 1107 58-59 0.4627139656202317 1107 60-61 0.36746426905303764 1107 62-63 0.3043069032367782 1107 64-65 0.332212703807258 1107 66-67 0.6029992915348643 1107 68-69 0.5040840187811995 1107 70-71 0.4579178094725478 1107 72-73 0.32438473004272694 1107 74-75 0.3257462722067537 1107 76-77 0.42125769096294974 1107 78-79 0.3736796682938568 1107 80-81 0.3999509026915291 1107 82-83 0.4549841861356967 1107 84-85 0.5956673123220213 1107 86-87 0.5584655527794276 1107 88-89 0.6308655904442766 1107 90-91 0.6900209466621305 1107 92-93 0.747560767718749 1107 94-95 0.7617870728779437 1107 96-97 0.8435660288713365 1107 98-99 0.9910350403180566 1107 100-101 1.1615654080695847 1108 1 -0.10357186057394685 1108 2 -0.10350169776946672 1108 3 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Count 2 9975.0 3 2295.0 4 1979.0 5 2167.0 6 2398.0 7 2943.0 8 3572.0 9 4394.0 10 5763.0 11 6967.0 12 9212.0 13 11492.0 14 12854.0 15 16022.0 16 19495.0 17 25121.0 18 21843.0 19 15644.0 20 15334.0 21 15957.0 22 17465.0 23 19473.0 24 21951.0 25 24813.0 26 28705.0 27 33681.0 28 39652.0 29 47479.0 30 58205.0 31 71548.0 32 90566.0 33 117006.0 34 157478.0 35 220193.0 36 324383.0 37 469084.0 38 400222.0 39 53915.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.867835144863186 19.450226564860486 15.292574364383494 22.389363925892837 2 14.84307645010593 20.401815848743876 41.04473123166274 23.71037646948746 3 18.934486143307648 25.043042415888728 30.084706472025562 25.937764968778065 4 12.333604419922734 17.047002404692595 38.33456763359786 32.2848255417868 5 13.406746626811906 37.59169699911363 34.43245812702118 14.569098247053292 6 30.853656551423036 36.695198267985575 18.12483643407714 14.326308746514247 7 27.755199817039223 33.1391461774803 21.145950448679585 17.95970355680089 8 25.9097292796581 34.78120963349562 20.205758311195883 19.103302775650402 9 26.008376673185378 17.15490251558941 20.987464203410596 35.84925660781461 10-11 24.22389961522928 27.143085241272217 27.733588803558217 20.899426339940284 12-13 25.393805667095247 24.50536513983255 28.220174311480505 21.880654881591695 14-15 22.542467104974854 25.798664541485483 26.873608897380706 24.785259456158958 16-17 21.462984747150276 29.146609621079655 27.377207266258857 22.01319836551121 18-19 21.317226497526946 27.619692708082276 30.042186855404694 21.020893938986084 20-21 22.342161583455518 26.555087867626114 30.08185315424361 21.020897394674755 22-23 21.820009677639458 26.55821367218008 30.031730889009495 21.590045761170966 24-25 21.67144029089064 26.70575697412312 30.121297624621217 21.501505110365024 26-27 21.42103507292054 26.93358192030515 30.038032888673943 21.60735011810037 28-29 21.347800429363666 27.11311779455316 30.04696373780103 21.492118038282147 30-31 21.54310202337086 26.8165091969126 30.026538064048648 21.613850715667894 32-33 21.33838431266489 26.879811984368757 29.974882834558514 21.80692086840784 34-35 21.7786866975935 26.948711933823144 29.847163449457838 21.42543791912552 36-37 21.71938573246813 26.667723829991445 29.854487032361714 21.758403405178708 38-39 21.44218882199338 27.04606887745255 29.888987765807062 21.622754534747006 40-41 21.82284056727639 26.950114303609666 29.709236737307414 21.51780839180653 42-43 21.46873463773644 26.99589449374743 29.547501266539083 21.987869601977053 44-45 21.67228148882339 27.13416625368424 29.392441117400036 21.80111114009233 46-47 21.870867591609695 27.32864636360582 28.890032889197357 21.910453155587124 48-49 21.814749811657023 27.42881052800728 28.821964234659696 21.934475425676002 50-51 21.79146759395783 27.629737179154063 28.64610105495231 21.932694171935793 52-53 22.003421726544097 27.68895269951513 28.36835078291408 21.939274791026694 54-55 21.844117299039343 27.555599426606292 28.529488058923736 22.07079521543063 56-57 21.91097537033788 27.81222180194416 28.380056176317613 21.89674665140035 58-59 22.0512316578247 27.491460555821217 28.50630454249192 21.951003243862157 60-61 21.936840734932492 27.534808402792976 28.733708286401612 21.79464257587292 62-63 21.684525032835676 27.52607293641198 28.921226713792862 21.868175316959483 64-65 21.76250204975576 27.465830102055932 28.66359679666465 22.108071051523662 66-67 21.6876493485306 27.629253890520094 28.456426216662443 22.226670544286858 68-69 21.725138641070302 27.578406811066586 28.64224480978693 22.05420973807618 70-71 21.836037198779124 27.388522274263906 28.608313079640048 22.167127447316926 72-73 21.816219874688635 27.427315674185447 28.52839576542644 22.22806868569948 74-75 21.714175429811384 27.818373393423467 28.254778000333836 22.212673176431313 76-77 21.774218283632308 27.56910470547757 28.335062702769697 22.321614308120427 78-79 21.81374788901839 27.54528986641195 28.269543819325428 22.37141842524423 80-81 21.731586376406693 27.837290554518678 28.14694802424096 22.28417504483367 82-83 21.84534190349678 27.67843861013302 28.305156848553835 22.17106263781636 84-85 21.833868368766392 27.649155556371486 28.19671501873806 22.320261056124064 86-87 21.769925572476087 27.6248240284325 28.281833225267803 22.32341717382361 88-89 21.905325779556904 27.396062556484342 28.142114807054085 22.55649685690467 90-91 21.890725901347118 27.321445560319862 28.35237619151738 22.435452346815637 92-93 21.8703339652593 27.241788143950608 28.42538193551267 22.46249595527742 94-95 21.684065590186506 27.29565652772562 28.415926065006047 22.60435181708182 96-97 21.62593264413544 27.354195633874657 28.418575590461657 22.601296131528247 98-99 21.595276753013888 27.452642138263215 28.190305225941138 22.761775882781755 100-101 21.715139954205302 27.178669417018803 28.506135721867302 22.60005490690859 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3308.0 1 1854.0 2 374.5 3 371.0 4 363.5 5 371.0 6 574.0 7 1111.5 8 1889.5 9 2455.5 10 2506.5 11 2465.0 12 2614.5 13 2799.5 14 3024.5 15 3199.5 16 3357.0 17 3551.0 18 3800.0 19 4066.5 20 4466.5 21 5229.5 22 6254.5 23 7516.0 24 8954.0 25 11274.5 26 14651.5 27 18338.5 28 21372.0 29 24804.0 30 29704.0 31 34617.5 32 40035.0 33 45642.5 34 51762.5 35 57641.5 36 63735.5 37 69655.5 38 73160.5 39 75616.0 40 77711.5 41 81781.0 42 85025.5 43 87860.0 44 92079.0 45 93936.5 46 94339.0 47 96193.0 48 99132.5 49 97254.5 50 90861.0 51 84074.0 52 78254.5 53 74867.0 54 71593.5 55 64745.0 56 56662.0 57 49972.0 58 44402.5 59 38738.5 60 31890.0 61 25503.0 62 20000.5 63 14948.0 64 11040.0 65 8459.0 66 6238.5 67 4605.0 68 3554.0 69 2535.5 70 1706.0 71 1144.5 72 830.0 73 674.0 74 480.0 75 358.5 76 289.0 77 184.5 78 122.0 79 74.0 80 44.0 81 41.5 82 29.5 83 18.0 84 18.5 85 16.0 86 10.0 87 13.5 88 14.5 89 6.5 90 5.0 91 6.5 92 6.5 93 8.5 94 8.5 95 8.5 96 10.0 97 9.0 98 10.5 99 11.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3474431714069637 2 0.3008425590607582 3 0.2959284641037857 4 0.2993016987776397 5 0.30109242829585847 6 0.22625659238331938 7 0.21297187805048692 8 0.21826077686010986 9 0.21147266263988512 10-11 0.23627218422358964 12-13 0.22373707759605807 14-15 0.2175944588998424 16-17 0.21978081470697 18-19 0.21365901844701274 20-21 0.20624623113903723 22-23 0.2086616337450068 24-25 0.19797972394446908 26-27 0.1989583784486119 28-29 0.21455438320612213 30-31 0.227047844961137 32-33 0.2200931512508454 34-35 0.22879692960683906 36-37 0.20430974456700993 38-39 0.20399740802313454 40-41 0.19937482717377908 42-43 0.20664185742794602 44-45 0.21107703635097633 46-47 0.21574126207284855 48-49 0.2232581615621158 50-51 0.22740182637752906 52-53 0.2487864684148629 54-55 0.22261266603810673 56-57 0.19550185402972448 58-59 0.19698024700406788 60-61 0.19158723601315314 62-63 0.20212338875988234 64-65 0.19277411487987953 66-67 0.1914414789593446 68-69 0.18779755261413195 70-71 0.1990416681936453 72-73 0.19762574252807696 74-75 0.20222750094117414 76-77 0.20449714649333514 78-79 0.2023524355587243 80-81 0.20399740802313454 82-83 0.19675120020522593 84-85 0.1886512725007246 86-87 0.18998390842125953 88-89 0.19048364689146013 90-91 0.1970635367491013 92-93 0.19217026422838715 94-95 0.18973403918615925 96-97 0.1877559077416152 98-99 0.1986043970322198 100-101 0.18623586989475507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2401256.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.24107554178851 #Duplication Level Percentage of deduplicated Percentage of total 1 78.34179587916009 45.627104518775376 2 12.489677720530533 14.548245272280234 3 3.953214255032198 6.907183501806164 4 1.706930893211997 3.976539643846898 5 0.9252082978678651 2.6942563184005945 6 0.5542800863718587 1.936912102901549 7 0.3723197620154555 1.5179012369670006 8 0.2693582764987856 1.255017258349738 9 0.18434210133594084 0.9662654024494707 >10 1.082265655012637 11.72264372909578 >50 0.0812187712289728 3.243372781286289 >100 0.037818173278985276 3.859228764969068 >500 9.278031777029136E-4 0.34865854354505493 >1k 4.995863264545438E-4 0.6659344928621308 >5k 1.4273895041558394E-4 0.7307364324646564 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9969 0.4151577341191443 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7611 0.3169591247247274 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4883 0.20335191249912543 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3540 0.14742284870917555 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.164487251671625E-5 0.0 0.0 0.0 0.0 7 8.32897450334325E-5 4.164487251671625E-5 0.0 0.0 0.0 8 8.32897450334325E-5 4.164487251671625E-5 0.0 0.0 0.0 9 8.32897450334325E-5 4.164487251671625E-5 0.0 0.0 0.0 10-11 8.32897450334325E-5 4.164487251671625E-5 0.0 0.0 0.0 12-13 1.2493461755014876E-4 4.164487251671625E-5 0.0 0.0 0.0 14-15 1.2493461755014876E-4 4.164487251671625E-5 0.0 0.0 0.0 16-17 1.2493461755014876E-4 4.164487251671625E-5 0.0 6.246730877507438E-5 0.0 18-19 1.2493461755014876E-4 4.164487251671625E-5 0.0 1.4575705380850688E-4 0.0 20-21 1.2493461755014876E-4 4.164487251671625E-5 0.0 2.0822436258358126E-4 0.0 22-23 1.2493461755014876E-4 4.164487251671625E-5 0.0 2.706916713586556E-4 0.0 24-25 1.2493461755014876E-4 6.246730877507438E-5 0.0 3.5398141639208814E-4 2.0822436258358125E-5 26-27 1.2493461755014876E-4 8.32897450334325E-5 0.0 6.246730877507438E-4 4.164487251671625E-5 28-29 1.8740192632522314E-4 8.32897450334325E-5 0.0 0.0013951032293099946 4.164487251671625E-5 30-31 2.0822436258358126E-4 8.32897450334325E-5 0.0 0.0026236269685531236 4.164487251671625E-5 32-33 2.0822436258358126E-4 1.0411218129179063E-4 0.0 0.004226954560446699 8.32897450334325E-5 34-35 2.0822436258358126E-4 1.2493461755014876E-4 0.0 0.0059343943336320655 8.32897450334325E-5 36-37 2.0822436258358126E-4 1.2493461755014876E-4 0.0 0.007808413596884297 8.32897450334325E-5 38-39 2.0822436258358126E-4 1.2493461755014876E-4 0.0 0.00966161042387817 8.32897450334325E-5 40-41 2.0822436258358126E-4 1.2493461755014876E-4 0.0 0.012347704701206368 8.32897450334325E-5 42-43 2.2904679884193938E-4 1.2493461755014876E-4 0.0 0.017157687476887097 8.32897450334325E-5 44-45 2.498692351002975E-4 1.2493461755014876E-4 0.0 0.02477869914744617 8.32897450334325E-5 46-47 3.3315898013373E-4 1.2493461755014876E-4 0.0 0.041665694952974605 8.32897450334325E-5 48-49 3.3315898013373E-4 1.2493461755014876E-4 0.0 0.07498159296634761 1.2493461755014876E-4 50-51 3.3315898013373E-4 1.2493461755014876E-4 0.0 0.10369573256662346 1.2493461755014876E-4 52-53 3.3315898013373E-4 1.66579490066865E-4 0.0 0.13170190933411513 1.2493461755014876E-4 54-55 3.3315898013373E-4 2.0822436258358126E-4 0.0 0.16989025743194394 1.2493461755014876E-4 56-57 3.3315898013373E-4 2.0822436258358126E-4 0.0 0.1990624906299037 1.2493461755014876E-4 58-59 3.3315898013373E-4 2.0822436258358126E-4 0.0 0.24333099011517306 1.2493461755014876E-4 60-61 3.3315898013373E-4 2.0822436258358126E-4 0.0 0.3145853669912746 1.66579490066865E-4 62-63 3.3315898013373E-4 2.0822436258358126E-4 0.0 0.3666831025096866 1.66579490066865E-4 64-65 3.3315898013373E-4 2.0822436258358126E-4 0.0 0.4330025619925572 1.66579490066865E-4 66-67 3.3315898013373E-4 2.0822436258358126E-4 0.0 0.5452979607338826 1.66579490066865E-4 68-69 3.748038526504463E-4 2.0822436258358126E-4 0.0 0.6678796429868369 1.66579490066865E-4 70-71 4.164487251671625E-4 2.0822436258358126E-4 0.0 0.78946184829939 1.66579490066865E-4 72-73 4.164487251671625E-4 2.0822436258358126E-4 0.0 0.9211221127609884 1.66579490066865E-4 74-75 4.164487251671625E-4 2.0822436258358126E-4 0.0 1.045286300169578 1.66579490066865E-4 76-77 4.164487251671625E-4 2.0822436258358126E-4 0.0 1.156290707862885 1.66579490066865E-4 78-79 4.164487251671625E-4 2.0822436258358126E-4 0.0 1.2961966570827934 1.66579490066865E-4 80-81 4.5809359768387875E-4 2.2904679884193938E-4 0.0 1.4566751733259595 1.66579490066865E-4 82-83 4.5809359768387875E-4 2.498692351002975E-4 0.0 1.661443011490653 1.66579490066865E-4 84-85 4.5809359768387875E-4 2.498692351002975E-4 0.0 1.9021087297647565 1.66579490066865E-4 86-87 4.5809359768387875E-4 2.498692351002975E-4 0.0 2.2790989382223303 1.66579490066865E-4 88-89 4.5809359768387875E-4 2.498692351002975E-4 0.0 2.7819815962979373 1.66579490066865E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11980 0.0 35.691116 1 GGTATCA 7475 0.0 34.231693 1 ATCAACG 14465 0.0 29.245945 3 TCAACGC 14490 0.0 29.100723 4 CAACGCA 14665 0.0 28.78527 5 TATCAAC 14810 0.0 28.660944 2 AACGCAG 15035 0.0 28.1178 6 GTATAGG 725 0.0 25.584911 1 ACGCAGA 17040 0.0 24.558458 7 CGCAGAG 17045 0.0 24.356182 8 GTGTACG 350 0.0 23.101393 1 GAGTACT 8650 0.0 22.734404 12-13 GCAGAGT 17900 0.0 22.582933 9 GTACATG 10470 0.0 22.349907 1 TACATGG 10350 0.0 22.227934 2 GTACTTT 8885 0.0 21.891834 14-15 AGTACTT 8950 0.0 21.14972 12-13 ACATGGG 10465 0.0 20.984413 3 AGAGTAC 14300 0.0 20.960283 10-11 TAGCCCT 1800 0.0 19.279638 4 >>END_MODULE