FastQCFastQC Report
Thu 26 May 2016
SRR937494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2401256
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122060.5083173139390386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98250.4091608724767372No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61910.25782340575099033No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.1094427249739303No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24610.1024880312636387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86600.035.122711
GTATCAA131550.032.8035621
ATCAACG162550.026.4179653
TCAACGC163950.026.2792994
TATCAAC165550.025.9679262
CAACGCA166950.025.8355265
AACGCAG170850.025.2426226
GTACATG102250.023.565171
TACATGG101700.023.0740552
ACATGGG100700.022.2182223
ACGCAGA194600.022.1181347
CGCAGAG195000.021.9753118
GAGTACT108250.021.70208712-13
AGTACTT111600.020.62493912-13
GTACTTT113550.020.53799414-15
AGAGTAC163150.020.39783910-11
GCAGAGT208950.019.9625159
CATGGGG49700.018.829024
TACTTTT126250.018.0769714-15
GTATAGG7400.017.9825921
Produced by FastQC (version 0.11.5)