##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937494_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2401256 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.155653124864656 33.0 31.0 34.0 31.0 34.0 2 32.39233800977488 34.0 31.0 34.0 31.0 34.0 3 32.464805501787396 34.0 31.0 34.0 31.0 34.0 4 36.085924616117566 37.0 35.0 37.0 35.0 37.0 5 35.93158663632699 37.0 35.0 37.0 35.0 37.0 6 36.01522952987936 37.0 35.0 37.0 35.0 37.0 7 35.979113847086694 37.0 35.0 37.0 35.0 37.0 8 35.95714992487265 37.0 35.0 37.0 35.0 37.0 9 37.652700086954496 39.0 37.0 39.0 35.0 39.0 10-11 37.57748090999044 39.0 37.0 39.0 35.0 39.0 12-13 37.48934078665498 39.0 37.0 39.0 35.0 39.0 14-15 38.70072849375494 40.0 38.0 41.0 34.0 41.0 16-17 38.681970810275956 40.0 38.0 41.0 34.5 41.0 18-19 38.69792350336657 40.0 38.0 41.0 34.0 41.0 20-21 38.61243261859627 40.0 38.0 41.0 34.0 41.0 22-23 38.48317255636217 40.0 38.0 41.0 34.0 41.0 24-25 38.34506878900042 40.0 38.0 41.0 34.0 41.0 26-27 38.12724153526321 40.0 38.0 41.0 33.5 41.0 28-29 38.11290258098262 40.0 38.0 41.0 33.0 41.0 30-31 38.241073629800404 40.0 38.0 41.0 34.0 41.0 32-33 38.19692798268906 40.0 38.0 41.0 34.0 41.0 34-35 38.086568237622316 40.0 37.5 41.0 33.5 41.0 36-37 37.93533259260987 40.0 37.0 41.0 33.0 41.0 38-39 37.79513679507724 40.0 37.0 41.0 33.0 41.0 40-41 37.64069782647081 40.0 37.0 41.0 33.0 41.0 42-43 37.433188506348344 40.0 37.0 41.0 32.5 41.0 44-45 37.15335057986321 40.0 37.0 41.0 31.5 41.0 46-47 36.81836255692854 40.0 36.0 41.0 31.0 41.0 48-49 36.49252766052433 39.5 36.0 41.0 30.0 41.0 50-51 36.274382239961085 39.0 36.0 41.0 30.0 41.0 52-53 36.047593009658286 39.0 35.5 41.0 29.5 41.0 54-55 35.84782234797123 39.0 35.0 40.0 29.0 41.0 56-57 35.60985375986567 39.0 35.0 40.0 28.0 41.0 58-59 35.36749580219685 38.0 35.0 40.0 28.0 41.0 60-61 35.04745120886736 38.0 34.0 40.0 27.0 41.0 62-63 34.691025238458536 38.0 34.0 40.0 26.5 41.0 64-65 34.35233915084439 37.0 34.0 40.0 26.0 41.0 66-67 33.968869416671936 37.0 33.5 39.0 26.0 41.0 68-69 33.528285822086445 36.0 33.0 39.0 25.0 40.0 70-71 33.13842359998267 36.0 33.0 39.0 24.5 40.0 72-73 32.70403176504296 35.0 32.0 38.0 23.5 40.0 74-75 32.25647452833017 35.0 32.0 37.0 22.0 39.0 76-77 30.924838084735654 34.0 30.5 36.0 20.5 38.5 78-79 31.551741255409667 35.0 31.0 36.0 22.0 39.0 80-81 31.37580520361011 35.0 31.0 36.0 20.5 37.5 82-83 31.033622404275093 34.0 31.0 35.5 20.0 37.0 84-85 30.69361471663163 34.0 31.0 35.0 18.5 37.0 86-87 30.3613956612706 34.0 31.0 35.0 17.5 36.0 88-89 29.99870963362507 34.0 30.5 35.0 13.0 36.0 90-91 29.555923233507798 34.0 30.0 35.0 7.0 35.5 92-93 29.147741015535203 34.0 29.5 35.0 2.0 35.0 94-95 28.750415407603356 34.0 29.0 35.0 2.0 35.0 96-97 28.330184495114224 34.0 29.0 35.0 2.0 35.0 98-99 27.700139427033186 33.5 28.5 35.0 2.0 35.0 100-101 26.12311661064043 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.021891463009758638 1101 2 0.0787984345271937 1101 3 0.05691245156815938 1101 4 0.04579681018556414 1101 5 -0.11487508092375975 1101 6 -0.03922613895608151 1101 7 0.02787635146783174 1101 8 -0.010138547430408096 1101 9 0.06990457366315184 1101 10-11 0.03379235293227367 1101 12-13 0.052376903889040705 1101 14-15 0.037341343463580756 1101 16-17 0.0697636612539867 1101 18-19 0.06724455354186176 1101 20-21 -0.0917598143341607 1101 22-23 -0.05814815463347145 1101 24-25 -0.03517349799643199 1101 26-27 -0.08219803080474719 1101 28-29 -0.10932074021509663 1101 30-31 0.04788105167909151 1101 32-33 0.030045397299012677 1101 34-35 0.055563589477046094 1101 36-37 -0.002511492521044545 1101 38-39 0.026964282877834478 1101 40-41 -0.07796734893330637 1101 42-43 -0.05045088896289229 1101 44-45 -0.05991933458744114 1101 46-47 -0.05995394069181259 1101 48-49 0.002478898188741141 1101 50-51 -0.029839036419666343 1101 52-53 0.031054945235965192 1101 54-55 0.005605322985758221 1101 56-57 0.10785873905718546 1101 58-59 0.09031082430887949 1101 60-61 0.12846745202133292 1101 62-63 -0.003572617541912848 1101 64-65 0.09605591467227015 1101 66-67 0.04496961035195568 1101 68-69 0.07619243885780236 1101 70-71 -0.16128243993679092 1101 72-73 -0.04305911387837824 1101 74-75 0.0925796734162816 1101 76-77 0.07670295848358322 1101 78-79 0.18969338506904876 1101 80-81 0.13964825136831038 1101 82-83 0.09139071620890604 1101 84-85 -0.012535406892560275 1101 86-87 -0.0535743289389039 1101 88-89 -0.10348086580259164 1101 90-91 0.07398306944135769 1101 92-93 0.10187826661850607 1101 94-95 -0.07779694721638819 1101 96-97 -0.1121146217788862 1101 98-99 -0.09735517826016249 1101 100-101 0.1686833466171258 1102 1 0.07297907887807042 1102 2 0.0052526197949021025 1102 3 -0.002591971052822828 1102 4 0.06957913569257812 1102 5 -0.08533196615102412 1102 6 -0.037153262514578955 1102 7 -0.014332735488572723 1102 8 0.027987726228928977 1102 9 0.08532320285549133 1102 10-11 0.02926256119787496 1102 12-13 -0.005891317534072016 1102 14-15 0.07056972792830862 1102 16-17 0.09291972962124362 1102 18-19 0.03199033212931823 1102 20-21 0.03507646047494717 1102 22-23 0.12147795449088505 1102 24-25 0.046701693630645025 1102 26-27 0.07932101197170027 1102 28-29 -0.03655217155048973 1102 30-31 0.01704408238700239 1102 32-33 0.09006651096955665 1102 34-35 0.19356247242328806 1102 36-37 0.07611070542841247 1102 38-39 0.11562881389367163 1102 40-41 0.17770072421009075 1102 42-43 0.27074993060632835 1102 44-45 0.21923895665401716 1102 46-47 0.30539119290531147 1102 48-49 0.38885853226030065 1102 50-51 0.2684588359910478 1102 52-53 0.2895286619291966 1102 54-55 0.406278479202804 1102 56-57 0.3407031590086049 1102 58-59 0.3742346829383081 1102 60-61 0.31206090260401 1102 62-63 0.3188694829949057 1102 64-65 0.37247158454769647 1102 66-67 0.3435947355703348 1102 68-69 0.3112681579879961 1102 70-71 0.2576443525694003 1102 72-73 0.241013999123453 1102 74-75 0.16621072916258584 1102 76-77 0.07248579973177627 1102 78-79 0.16890002911050317 1102 80-81 0.2203329184261591 1102 82-83 0.20822079452491238 1102 84-85 0.13884053340172642 1102 86-87 0.12109702234922182 1102 88-89 0.217452088835973 1102 90-91 0.13300714408076786 1102 92-93 0.052555973978837045 1102 94-95 0.11844864040476821 1102 96-97 0.14683634547534652 1102 98-99 0.12013735158740957 1102 100-101 0.32853011267878074 1103 1 -0.0639292822562112 1103 2 -0.043558889796422307 1103 3 -0.001794431436472621 1103 4 0.033324965550797 1103 5 -0.11664191193918327 1103 6 -0.07199219078814423 1103 7 -0.062080858924773 1103 8 -0.06813925458841652 1103 9 -0.08328344852066039 1103 10-11 -0.06640309768453534 1103 12-13 -0.07337072745901452 1103 14-15 -0.07056090176310192 1103 16-17 -0.23412754936124003 1103 18-19 -0.1844243884711787 1103 20-21 -0.2597186813098773 1103 22-23 -0.16113516060502775 1103 24-25 -0.2583611191299795 1103 26-27 -0.1522925722651678 1103 28-29 -0.21215293260136292 1103 30-31 -0.12998338842200496 1103 32-33 -0.028480194618431653 1103 34-35 -0.016091405808566606 1103 36-37 -0.06286448222796537 1103 38-39 -0.024057904204738634 1103 40-41 0.05112258617839416 1103 42-43 0.06198351067305197 1103 44-45 -0.009718768533637956 1103 46-47 -0.024770660722708726 1103 48-49 0.021052026839115 1103 50-51 -0.025240340405943584 1103 52-53 -0.042904615802250134 1103 54-55 0.01303932157144061 1103 56-57 -0.015363094119003051 1103 58-59 -0.1450000960441784 1103 60-61 -0.13138676211659117 1103 62-63 0.005872975488649956 1103 64-65 0.0033462717870662573 1103 66-67 -0.04006352131040103 1103 68-69 -0.01559798046071137 1103 70-71 -0.012291531500657982 1103 72-73 -0.09114561305336366 1103 74-75 -0.20528244223024217 1103 76-77 -0.3715300468070062 1103 78-79 0.057652374815255314 1103 80-81 0.005323014339420951 1103 82-83 -0.04735155743505004 1103 84-85 -0.10451460629802511 1103 86-87 0.0750508380289574 1103 88-89 -0.04569688947928796 1103 90-91 -0.07665324999763001 1103 92-93 0.0077895019187899095 1103 94-95 -0.1383630255668784 1103 96-97 -0.0065610169099734605 1103 98-99 0.0050609858659740325 1103 100-101 0.2335017035878728 1104 1 -0.0829189990334882 1104 2 0.021668511551524716 1104 3 0.0018217387544012809 1104 4 9.22202778760095E-4 1104 5 -0.16685879854720298 1104 6 -0.038418819125851655 1104 7 -0.07592526649795417 1104 8 -0.0323093940132253 1104 9 -0.017895432108659293 1104 10-11 0.009267659119501559 1104 12-13 -0.045597263832938495 1104 14-15 0.0313447742671471 1104 16-17 -0.03852211578095677 1104 18-19 -0.01652826895685422 1104 20-21 -0.1792542320635917 1104 22-23 -0.23561312145289293 1104 24-25 -0.24162041518904687 1104 26-27 -0.12794122239121464 1104 28-29 -0.21968632574331082 1104 30-31 -0.2044532300899533 1104 32-33 -0.14822652249825552 1104 34-35 -0.04559779182492463 1104 36-37 -0.07818384735632833 1104 38-39 -0.22782118428911247 1104 40-41 -0.1446635594459309 1104 42-43 -0.09085747880578054 1104 44-45 -0.012791275221914589 1104 46-47 0.07367069789744107 1104 48-49 -0.0942186232097626 1104 50-51 -0.06588937639401138 1104 52-53 0.08827303506979689 1104 54-55 0.049323856221853646 1104 56-57 -0.028436340376138958 1104 58-59 -0.03571593439371412 1104 60-61 -0.12153988502650037 1104 62-63 -0.019914424187227553 1104 64-65 0.011710313305819398 1104 66-67 -0.05602277428153002 1104 68-69 -0.04111374935508394 1104 70-71 -0.027761865142686304 1104 72-73 -0.033898320518503056 1104 74-75 -0.12525409744966964 1104 76-77 -0.008389676636713972 1104 78-79 0.06777329432443935 1104 80-81 0.025879009348937387 1104 82-83 -0.04986384070922156 1104 84-85 -0.06457684262564811 1104 86-87 -0.044161874838255955 1104 88-89 0.09963160771678048 1104 90-91 0.05511903031826293 1104 92-93 -0.03694589320391728 1104 94-95 -0.2787725055535013 1104 96-97 -0.18926563632140514 1104 98-99 -0.09159302774209266 1104 100-101 0.15637214720056747 1105 1 -0.05488722956368264 1105 2 -0.03309276356596769 1105 3 -0.06394700815911136 1105 4 -0.0737610217103537 1105 5 -0.21911549329413305 1105 6 -0.09748618326064218 1105 7 -0.08236531173311334 1105 8 -0.05385147230700227 1105 9 -0.06378974556756134 1105 10-11 -0.01432579600531625 1105 12-13 -0.2001594144885459 1105 14-15 -0.06627782417190531 1105 16-17 -0.10547327171213539 1105 18-19 -0.19188494145323176 1105 20-21 -0.08623142016337937 1105 22-23 -0.151818064484587 1105 24-25 -0.1871945369064747 1105 26-27 -0.20158958297738394 1105 28-29 -0.33147382866390274 1105 30-31 -0.2792586851793857 1105 32-33 -0.3022804986138041 1105 34-35 -0.12389214408302962 1105 36-37 -0.09985782328181614 1105 38-39 -0.124095903625701 1105 40-41 0.004033160874399755 1105 42-43 -0.1749003558585045 1105 44-45 -0.02060379314279004 1105 46-47 0.08760719828134 1105 48-49 -0.1277157062240022 1105 50-51 -0.07296670838510266 1105 52-53 -0.056106700341914006 1105 54-55 0.0058809544608493525 1105 56-57 0.026022799048341483 1105 58-59 -0.003443205482504652 1105 60-61 -0.004819738375999805 1105 62-63 -0.10437691594157883 1105 64-65 0.01451629205342897 1105 66-67 -0.09084522368445391 1105 68-69 -0.1834902792447224 1105 70-71 -0.03207475385155334 1105 72-73 -0.015944758971137674 1105 74-75 -0.13695857859387672 1105 76-77 -0.12720281750764073 1105 78-79 -0.11603121667287297 1105 80-81 -0.09715780500047444 1105 82-83 -0.1273421480546233 1105 84-85 -0.30596310803514015 1105 86-87 -0.24400677633120793 1105 88-89 -0.07841258143064778 1105 90-91 -0.08029486117307627 1105 92-93 -0.14246563705052395 1105 94-95 -0.04377975255008337 1105 96-97 -0.14069625865866797 1105 98-99 -0.03905000863315777 1105 100-101 -0.05121981494137273 1106 1 -0.2672451088275203 1106 2 -0.12225851845823144 1106 3 -0.16532592641652855 1106 4 -0.030454088224473708 1106 5 -0.1859029317438896 1106 6 -0.16305299741721768 1106 7 -0.1130616387349761 1106 8 -0.11864468712864351 1106 9 -0.2247026996553032 1106 10-11 -0.1990138114790554 1106 12-13 -0.37493881472348534 1106 14-15 -0.19366338078936707 1106 16-17 -0.12756745618286658 1106 18-19 -0.17099561319955825 1106 20-21 -0.3375054369143342 1106 22-23 -0.3344310449400538 1106 24-25 -0.21946451790594068 1106 26-27 -0.18433059145097275 1106 28-29 -0.265547195770111 1106 30-31 -0.1371709743799201 1106 32-33 -0.08762310824791797 1106 34-35 -0.14108066106918926 1106 36-37 -0.1736315706614704 1106 38-39 -0.1156533690651429 1106 40-41 -0.21635588136390282 1106 42-43 -0.3340914360330416 1106 44-45 -0.21732582911678122 1106 46-47 -4.035368104382542E-5 1106 48-49 -0.24230884167965883 1106 50-51 -0.042766944380637995 1106 52-53 -0.04066971001212494 1106 54-55 -0.11210336856746039 1106 56-57 -0.19884735522476404 1106 58-59 -0.1689075709444623 1106 60-61 -0.03979946325811312 1106 62-63 -0.00772062408500318 1106 64-65 -0.18953426390265804 1106 66-67 0.1237034983567753 1106 68-69 0.14198006932611662 1106 70-71 -0.009622108450180633 1106 72-73 0.08239954923518411 1106 74-75 0.022276739794236278 1106 76-77 0.02553749892260626 1106 78-79 0.0895958402945567 1106 80-81 0.04734483045824689 1106 82-83 -0.0362243053376794 1106 84-85 -0.03506598334382005 1106 86-87 0.03658123274770375 1106 88-89 0.048423589134383604 1106 90-91 0.08819662450381216 1106 92-93 0.1274545239240119 1106 94-95 0.06240479350890027 1106 96-97 0.17518302620651838 1106 98-99 0.15787460331887004 1106 100-101 0.14008930329905311 1107 1 -0.15813422625243945 1107 2 -0.08100032149677361 1107 3 -0.07374298836901971 1107 4 -0.10390679856797647 1107 5 -0.19612126054879297 1107 6 -0.11626176784877629 1107 7 -0.22447359922807664 1107 8 -0.08367267664059597 1107 9 -0.234452821805192 1107 10-11 -0.11767808451532602 1107 12-13 -0.1523035974443161 1107 14-15 -0.139450651270991 1107 16-17 -0.17519133509136253 1107 18-19 -0.18239017973237281 1107 20-21 -0.191113022711086 1107 22-23 -0.2547627389964404 1107 24-25 -0.1359034251961333 1107 26-27 -0.12408054289090842 1107 28-29 -0.2989466907454812 1107 30-31 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-0.5910228233168979 2316 60-61 -0.6409790081058944 2316 62-63 -0.5813345779872847 2316 64-65 -0.6144177656460883 2316 66-67 -0.7002239491178841 2316 68-69 -0.6931380874125779 2316 70-71 -0.7762119860461141 2316 72-73 -0.6309350515560368 2316 74-75 -0.8622259582730223 2316 76-77 -0.7230507687321399 2316 78-79 -0.46297763855372764 2316 80-81 -0.6914517182809021 2316 82-83 -0.9565427339056392 2316 84-85 -0.8361071865119243 2316 86-87 -0.7151229587624854 2316 88-89 -1.1011781729017507 2316 90-91 -1.236120161762333 2316 92-93 -1.1662913804341528 2316 94-95 -1.3696380924117975 2316 96-97 -1.2501856826853732 2316 98-99 -1.086641888465575 2316 100-101 -1.3259652081768358 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 9.0 4 13.0 5 51.0 6 84.0 7 224.0 8 534.0 9 1115.0 10 1942.0 11 3153.0 12 4851.0 13 6921.0 14 8840.0 15 12341.0 16 16700.0 17 23611.0 18 21651.0 19 12950.0 20 10767.0 21 10750.0 22 11135.0 23 12337.0 24 14384.0 25 16796.0 26 20737.0 27 25734.0 28 32258.0 29 40649.0 30 50958.0 31 64453.0 32 82981.0 33 108296.0 34 148910.0 35 213827.0 36 333924.0 37 541626.0 38 493249.0 39 52484.0 40 3.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.931537897473504 20.30056329578028 15.797238517432172 21.970660289314043 2 15.417764703138689 21.232721542392817 39.93139423701596 23.41811951745253 3 19.210779691961207 25.25032732869798 30.433864610853657 25.105028368487158 4 12.951305483463655 17.852240660720888 37.641634211429356 31.554819644386107 5 13.454272989703774 37.87674767396772 34.23393893402561 14.435040402302896 6 30.914529729441593 37.30127066835023 17.548816119564094 14.235383482644082 7 27.68900941840437 33.49892722808397 20.951410428542395 17.860652924969266 8 26.23881002275476 35.141401000143254 19.755244755244757 18.864544221857226 9 26.11212632055891 17.190503636430268 20.73423241836772 35.9631376246431 10-11 24.40874692244392 27.501420090152823 27.309791209267146 20.78004177813611 12-13 25.63099598723077 24.719928698900706 28.09990066990548 21.549174643963042 14-15 22.609001289325253 26.042912542436124 26.792207911193145 24.555878257045478 16-17 21.407005333875272 29.469244428748954 27.354413690168812 21.76933654720696 18-19 21.342809762890756 27.664834569908415 30.159466545841006 20.83288912135982 20-21 22.23846603610777 26.595061084699008 30.42516083249766 20.741312046695562 22-23 21.92353245922503 26.54181527118624 30.280548119545497 21.254104150043226 24-25 21.79750050546685 26.76238227203341 30.396829478701783 21.043287743797958 26-27 21.473346398946532 27.01566790798321 30.317747606002634 21.193238087067627 28-29 21.32517962901034 27.242181926612496 30.300299975137424 21.13233846923974 30-31 21.593999591460484 26.849521069665567 30.30574039666647 21.250738942207484 32-33 21.38624536492569 26.94377442471773 30.29083529619499 21.37914491416159 34-35 21.842506588218832 26.975028901541524 30.128982499158774 21.053482011080867 36-37 21.88585827015621 26.668718374564264 30.11052778103418 21.334895574245348 38-39 21.596330745167986 27.116912625317518 30.131300337053545 21.15545629246095 40-41 21.82641084499112 27.02954203966591 29.960862148808793 21.18318496653418 42-43 21.57774356434757 27.01673489337412 29.809181106661697 21.59634043561661 44-45 21.801111584937217 27.146272617330265 29.61997804482321 21.43263775290931 46-47 22.021433782986904 27.4154234284058 29.082238628451112 21.480904160156186 48-49 22.028909037603654 27.537255502953457 28.86108353295109 21.572751926491804 50-51 22.054520634201435 27.745250818738192 28.707018327075495 21.493210219984874 52-53 22.20078667196945 27.850374075223165 28.35908722730405 21.58975202550333 54-55 22.026764326669042 27.769030040945236 28.50776843451927 21.69643719786645 56-57 22.18139173832361 28.023896660747543 28.272037633638398 21.52267396729045 58-59 22.24835669333049 27.70941540593756 28.430454728691984 21.611773172039968 60-61 22.123505365525375 27.721263372168565 28.71951178883051 21.435719473475547 62-63 21.891314378808424 27.73042524412224 28.82949589714716 21.548764479922173 64-65 21.944078432287103 27.66806204752846 28.664082463510766 21.723777056673672 66-67 21.95061667727223 27.75455844774568 28.399387653794516 21.895437221187578 68-69 21.961881615287997 27.721534063839925 28.559241496949927 21.757342823922148 70-71 22.067701236352974 27.610425543965324 28.456337016961125 21.865536202720577 72-73 22.078341501280995 27.54189890623907 28.420938875321916 21.95882071715802 74-75 21.948888415062783 28.023792548566252 28.046405714342825 21.98091332202814 76-77 22.00127766468881 27.772278341001545 28.22912259250992 21.997321401799724 78-79 21.98205855602235 27.7888530002632 28.143625669233103 22.085462774481353 80-81 21.940767664922024 28.00340738346932 28.078014172583014 21.977810779025646 82-83 22.01266007287871 27.884664237376366 28.162019781363874 21.94065590838105 84-85 21.95910055366881 27.88239899552526 28.041607409458802 22.11689304134713 86-87 21.896957259034437 27.81985760785189 28.064875215303992 22.218309917809677 88-89 21.982391715002482 27.62216939801504 28.03103875638416 22.36440013059832 90-91 21.972180244295913 27.53869275010844 28.192403074720847 22.296723930874798 92-93 21.881875482832505 27.469114336819793 28.295723587630327 22.353286592717375 94-95 21.77402159536509 27.567323100910524 28.221876384692013 22.436778919032374 96-97 21.683693866876336 27.629394783396688 28.226124161688716 22.46078718803826 98-99 21.592221632013846 27.669748359534214 27.94700285083833 22.79102715761361 100-101 21.435382665210867 27.746312479188095 28.117620819423156 22.700684036177883 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 338.0 1 258.0 2 222.5 3 309.5 4 315.0 5 316.0 6 551.0 7 1202.0 8 2135.0 9 2819.0 10 2829.5 11 2698.0 12 2912.0 13 3190.5 14 3383.0 15 3540.0 16 3735.0 17 4008.0 18 4321.5 19 4613.5 20 4992.5 21 5621.0 22 6572.5 23 7815.0 24 9278.0 25 11525.0 26 14892.5 27 18619.0 28 21576.5 29 24954.0 30 29741.0 31 34813.5 32 40028.5 33 45452.5 34 51750.5 35 57449.0 36 63554.5 37 69474.5 38 73480.0 39 76076.5 40 77980.0 41 81933.0 42 84857.5 43 87957.5 44 92699.0 45 94652.0 46 94666.0 47 96556.5 48 99655.0 49 97380.5 50 90892.5 51 84033.5 52 77810.5 53 74563.0 54 71596.5 55 64563.0 56 56095.0 57 49135.5 58 43679.0 59 38333.5 60 31423.0 61 25071.0 62 19783.5 63 14764.5 64 10672.5 65 7981.0 66 5883.5 67 4438.5 68 3367.0 69 2287.0 70 1525.5 71 1034.5 72 766.0 73 617.0 74 464.0 75 349.5 76 233.0 77 133.5 78 84.5 79 60.0 80 40.5 81 22.0 82 9.0 83 6.0 84 4.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04564278027832101 2 0.0 3 0.0 4 0.0 5 0.011618919432163834 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007121273200358479 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001166056430468055 24-25 4.3727116142552066E-4 26-27 0.0017490846457020827 28-29 0.0023737577334528265 30-31 8.12075014075967E-4 32-33 0.0 34-35 0.0 36-37 1.8740192632522311E-4 38-39 2.2904679884193938E-4 40-41 0.0 42-43 0.013097312406507262 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.164487251671625E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.498692351002975E-4 84-85 4.164487251671625E-5 86-87 0.0 88-89 0.0 90-91 0.008016637959467878 92-93 0.002540337223519691 94-95 0.0 96-97 0.0 98-99 0.0013534583567932782 100-101 0.011931255976039206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2401256.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.203145485514085 #Duplication Level Percentage of deduplicated Percentage of total 1 76.85618667033242 43.19559440894521 2 13.091085480745832 14.715203636753182 3 4.323294253020458 7.289482076375872 4 1.915741036206424 4.306826886819166 5 1.00974540713821 2.8375434010359237 6 0.6047439215595005 2.0393106362933353 7 0.41846563294073175 1.6463359394198975 8 0.28190068385101474 1.2674964117555592 9 0.20867513002549806 1.0555378822828472 >10 1.1586343042632676 12.054418573793203 >50 0.08804989318919373 3.3979470633871314 >100 0.041410207375397676 4.132744832883609 >500 0.0015505345141153747 0.5703842297504662 >1k 2.9533990745021305E-4 0.32002933283605794 >5k 1.4766995372510652E-4 0.6646252327155477 >10k+ 7.383497686255326E-5 0.5065194549529205 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12206 0.5083173139390386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9825 0.4091608724767372 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6191 0.25782340575099033 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2628 0.1094427249739303 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2461 0.1024880312636387 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.164487251671625E-5 6 0.0 0.0 0.0 0.0 4.164487251671625E-5 7 4.164487251671625E-5 0.0 0.0 0.0 4.164487251671625E-5 8 4.164487251671625E-5 0.0 0.0 0.0 4.164487251671625E-5 9 4.164487251671625E-5 0.0 0.0 0.0 4.164487251671625E-5 10-11 8.32897450334325E-5 4.164487251671625E-5 0.0 0.0 4.164487251671625E-5 12-13 8.32897450334325E-5 4.164487251671625E-5 0.0 0.0 4.164487251671625E-5 14-15 1.66579490066865E-4 4.164487251671625E-5 0.0 0.0 4.164487251671625E-5 16-17 1.66579490066865E-4 4.164487251671625E-5 0.0 6.246730877507438E-5 4.164487251671625E-5 18-19 1.66579490066865E-4 4.164487251671625E-5 0.0 1.0411218129179063E-4 4.164487251671625E-5 20-21 1.66579490066865E-4 8.32897450334325E-5 0.0 1.66579490066865E-4 4.164487251671625E-5 22-23 1.66579490066865E-4 8.32897450334325E-5 0.0 2.2904679884193938E-4 6.246730877507438E-5 24-25 1.66579490066865E-4 1.0411218129179063E-4 0.0 3.123365438753719E-4 8.32897450334325E-5 26-27 1.66579490066865E-4 1.2493461755014876E-4 0.0 5.830282152340275E-4 8.32897450334325E-5 28-29 1.66579490066865E-4 1.2493461755014876E-4 0.0 0.0012701686117598458 8.32897450334325E-5 30-31 1.66579490066865E-4 1.2493461755014876E-4 0.0 0.0025819820960364076 8.32897450334325E-5 32-33 1.66579490066865E-4 1.66579490066865E-4 0.0 0.004268599432963416 8.32897450334325E-5 34-35 1.66579490066865E-4 1.66579490066865E-4 0.0 0.0059343943336320655 8.32897450334325E-5 36-37 1.66579490066865E-4 1.66579490066865E-4 0.0 0.007850058469401013 8.32897450334325E-5 38-39 1.66579490066865E-4 1.66579490066865E-4 0.0 0.00978654504142832 8.32897450334325E-5 40-41 1.66579490066865E-4 1.66579490066865E-4 0.0 0.012472639318756517 8.32897450334325E-5 42-43 1.66579490066865E-4 1.66579490066865E-4 0.0 0.01734508940321232 8.32897450334325E-5 44-45 2.0822436258358126E-4 1.66579490066865E-4 0.0 0.025070213255063183 8.32897450334325E-5 46-47 2.0822436258358126E-4 1.66579490066865E-4 0.0 0.0417698071342664 8.32897450334325E-5 48-49 2.0822436258358126E-4 1.66579490066865E-4 0.0 0.0751898173289312 8.32897450334325E-5 50-51 2.0822436258358126E-4 1.66579490066865E-4 0.0 0.10384148962043196 8.32897450334325E-5 52-53 2.0822436258358126E-4 2.0822436258358126E-4 0.0 0.13199342344173215 8.32897450334325E-5 54-55 2.0822436258358126E-4 2.0822436258358126E-4 0.0 0.17034835102962784 8.32897450334325E-5 56-57 2.0822436258358126E-4 2.0822436258358126E-4 0.0 0.19985374320772128 8.32897450334325E-5 58-59 2.0822436258358126E-4 2.0822436258358126E-4 0.0 0.24433046705557426 8.32897450334325E-5 60-61 2.0822436258358126E-4 2.0822436258358126E-4 0.0 0.3162511618919432 8.32897450334325E-5 62-63 2.0822436258358126E-4 2.0822436258358126E-4 0.0 0.3690152153706227 8.32897450334325E-5 64-65 2.0822436258358126E-4 2.0822436258358126E-4 0.0 0.43548043190730185 8.32897450334325E-5 66-67 2.498692351002975E-4 2.0822436258358126E-4 0.0 0.5475884287223021 8.32897450334325E-5 68-69 2.498692351002975E-4 2.0822436258358126E-4 0.0 0.6706906718817152 8.32897450334325E-5 70-71 2.498692351002975E-4 2.0822436258358126E-4 0.0 0.7933764663159613 1.0411218129179063E-4 72-73 2.498692351002975E-4 2.0822436258358126E-4 0.0 0.9260778525904776 1.2493461755014876E-4 74-75 2.498692351002975E-4 2.498692351002975E-4 0.0 1.0515538534833437 1.2493461755014876E-4 76-77 2.498692351002975E-4 2.498692351002975E-4 0.0 1.1632662240094351 1.2493461755014876E-4 78-79 2.498692351002975E-4 2.498692351002975E-4 0.0 1.304837968130012 1.2493461755014876E-4 80-81 3.3315898013373E-4 2.498692351002975E-4 0.0 1.4675028401803059 1.2493461755014876E-4 82-83 3.748038526504463E-4 2.498692351002975E-4 0.0 1.674394566843352 1.2493461755014876E-4 84-85 3.956262889088044E-4 2.498692351002975E-4 0.0 1.9177047345222666 1.2493461755014876E-4 86-87 4.164487251671625E-4 2.498692351002975E-4 0.0 2.2992967013929375 1.2493461755014876E-4 88-89 4.164487251671625E-4 2.498692351002975E-4 0.0 2.8119450820737146 1.2493461755014876E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8660 0.0 35.12271 1 GTATCAA 13155 0.0 32.803562 1 ATCAACG 16255 0.0 26.417965 3 TCAACGC 16395 0.0 26.279299 4 TATCAAC 16555 0.0 25.967926 2 CAACGCA 16695 0.0 25.835526 5 AACGCAG 17085 0.0 25.242622 6 GTACATG 10225 0.0 23.56517 1 TACATGG 10170 0.0 23.074055 2 ACATGGG 10070 0.0 22.218222 3 ACGCAGA 19460 0.0 22.118134 7 CGCAGAG 19500 0.0 21.975311 8 GAGTACT 10825 0.0 21.702087 12-13 AGTACTT 11160 0.0 20.624939 12-13 GTACTTT 11355 0.0 20.537994 14-15 AGAGTAC 16315 0.0 20.397839 10-11 GCAGAGT 20895 0.0 19.962515 9 CATGGGG 4970 0.0 18.82902 4 TACTTTT 12625 0.0 18.07697 14-15 GTATAGG 740 0.0 17.982592 1 >>END_MODULE