FastQCFastQC Report
Thu 26 May 2016
SRR937493_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937493_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629906
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109200.4152239661797798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81200.30875628254393883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52350.19905654422629557No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39000.14829427363563563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81950.035.6838951
GTATCAA129450.034.344381
ATCAACG160250.027.4955313
TATCAAC161700.027.4258652
TCAACGC160750.027.380444
CAACGCA162500.027.0829935
AACGCAG165900.026.5725756
GTACATG113650.024.3500391
ACGCAGA185800.023.470867
CGCAGAG186150.023.37488
TACATGG113400.023.1793252
ACATGGG114650.022.0140973
AGAGTAC157100.021.4821910-11
GAGTACT97100.021.47472812-13
GCAGAGT197800.021.275999
GTACTTT98850.021.0701214-15
AGTACTT100600.020.89284712-13
CATGGGG53900.020.8115144
GCACCGT3850.019.7420066
TATCCCG1951.0284439E-519.4981965
Produced by FastQC (version 0.11.5)