##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937493_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2629906 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.53487615146701 31.0 31.0 34.0 30.0 34.0 2 31.629189028048913 33.0 31.0 34.0 30.0 34.0 3 31.671372284788887 33.0 31.0 34.0 30.0 34.0 4 35.302678118533514 37.0 35.0 37.0 33.0 37.0 5 35.293427597792466 37.0 35.0 37.0 33.0 37.0 6 35.33952278142261 37.0 35.0 37.0 33.0 37.0 7 35.27628706121055 37.0 35.0 37.0 33.0 37.0 8 35.26044809206108 37.0 35.0 37.0 33.0 37.0 9 36.85445107163526 39.0 37.0 39.0 33.0 39.0 10-11 36.75285390428403 39.0 37.0 39.0 33.0 39.0 12-13 36.637564422454645 39.0 37.0 39.0 32.0 39.0 14-15 37.761621708152305 40.0 37.5 41.0 32.5 41.0 16-17 37.7057256038809 40.0 37.0 41.0 32.0 41.0 18-19 37.67278982594815 40.0 37.0 41.0 32.0 41.0 20-21 37.60806374828606 40.0 37.0 41.0 32.0 41.0 22-23 37.46775740273607 40.0 37.0 41.0 32.0 41.0 24-25 37.319138782907075 40.0 37.0 41.0 32.0 41.0 26-27 37.14916141489468 39.0 36.5 41.0 31.0 41.0 28-29 36.962571285817816 39.0 36.0 41.0 31.0 41.0 30-31 36.95719143573953 39.0 36.0 41.0 31.0 41.0 32-33 36.80636209050817 39.0 36.0 41.0 30.0 41.0 34-35 36.601881398042366 39.0 35.5 41.0 30.0 41.0 36-37 36.62239886140417 39.0 36.0 41.0 30.0 41.0 38-39 36.60049864900114 39.0 36.0 41.0 30.0 41.0 40-41 36.44530641019109 39.0 35.5 41.0 30.0 41.0 42-43 36.21493239682331 39.0 35.0 41.0 30.0 41.0 44-45 35.99326782021867 39.0 35.0 41.0 29.0 41.0 46-47 35.675765787826634 39.0 35.0 41.0 27.5 41.0 48-49 35.36683022130829 39.0 35.0 40.0 26.0 41.0 50-51 34.441888037062924 38.0 34.0 39.5 25.0 40.5 52-53 34.574528899511996 38.0 34.0 39.5 25.0 40.5 54-55 34.934623328742546 39.0 35.0 40.0 25.5 41.0 56-57 34.71819106842602 38.0 34.0 40.0 24.5 41.0 58-59 34.48215962851904 38.0 34.0 40.0 23.5 41.0 60-61 34.2075779514553 38.0 34.0 40.0 23.0 41.0 62-63 33.89639591681224 38.0 33.5 40.0 22.0 41.0 64-65 33.55003581877071 37.0 33.0 40.0 22.0 41.0 66-67 33.156466048596414 36.5 32.5 39.5 20.5 41.0 68-69 32.6664205868955 36.0 32.0 39.0 18.5 40.5 70-71 32.2392017053081 35.5 31.5 39.0 17.5 40.0 72-73 31.828041002225934 35.0 31.0 38.0 14.0 40.0 74-75 31.369468718653824 35.0 31.0 37.0 10.0 39.5 76-77 30.928094007922716 35.0 30.5 37.0 7.5 39.0 78-79 30.449598388687654 34.5 30.0 36.0 7.0 39.0 80-81 29.992264552421265 34.0 30.0 36.0 2.0 37.5 82-83 29.5424429618397 34.0 29.0 35.0 2.0 37.0 84-85 29.068355484948892 34.0 29.0 35.0 2.0 37.0 86-87 28.64714499301496 34.0 29.0 35.0 2.0 36.0 88-89 28.25358605972989 34.0 28.5 35.0 2.0 36.0 90-91 27.813924147859275 34.0 27.0 35.0 2.0 35.5 92-93 27.36237568947331 33.0 26.0 35.0 2.0 35.0 94-95 26.82341308016332 33.0 25.5 35.0 2.0 35.0 96-97 26.192152114942512 33.0 24.5 35.0 2.0 35.0 98-99 25.48402699564167 32.0 21.5 34.5 2.0 35.0 100-101 23.68033667363016 30.5 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.035584639159164766 1101 2 -0.014187804095694645 1101 3 0.055294211480045874 1101 4 -0.06656472995285867 1101 5 0.09462791351374022 1101 6 -0.10069138660158927 1101 7 0.12502391842034655 1101 8 -0.04921414967699178 1101 9 -0.023623871087998793 1101 10-11 -0.03930506125869471 1101 12-13 0.016694369086359018 1101 14-15 0.08351230671318177 1101 16-17 -0.021680776709089855 1101 18-19 0.15481655618425094 1101 20-21 0.09920774986999703 1101 22-23 0.01726787681612052 1101 24-25 0.14579909986449024 1101 26-27 0.18682287086487293 1101 28-29 0.052510730903208014 1101 30-31 0.015081502397464419 1101 32-33 0.011758543267980315 1101 34-35 -0.06945340266496913 1101 36-37 0.00423583160907981 1101 38-39 0.11874705699925414 1101 40-41 0.07446840926170495 1101 42-43 0.015630702071007363 1101 44-45 -0.1110625971149517 1101 46-47 0.0936742849945631 1101 48-49 0.2297167057400955 1101 50-51 0.09003944140707887 1101 52-53 0.09642930247919423 1101 54-55 0.20664240190810546 1101 56-57 0.35279876705087077 1101 58-59 0.3232084385682654 1101 60-61 0.3403170869269374 1101 62-63 0.43673573993074655 1101 64-65 0.24631253430769107 1101 66-67 0.4675403545168493 1101 68-69 0.3245531651569067 1101 70-71 0.35041805287738725 1101 72-73 0.4207388135426662 1101 74-75 0.46361647668673456 1101 76-77 0.47131695167065857 1101 78-79 0.4500128544658075 1101 80-81 0.5193783344505647 1101 82-83 0.5010957731177861 1101 84-85 0.4185569174395276 1101 86-87 0.5085595554831812 1101 88-89 0.4600702483907497 1101 90-91 0.4630986134721269 1101 92-93 0.4807485352909424 1101 94-95 0.4155000229215595 1101 96-97 0.4559234454556247 1101 98-99 0.5689568989713436 1101 100-101 1.0609972021852592 1102 1 -0.002952317092162815 1102 2 -0.09700609362674584 1102 3 -0.07434927120028689 1102 4 -0.32580819039385034 1102 5 -0.241422854371649 1102 6 -0.13409546909068126 1102 7 -0.21411499200286954 1102 8 -0.13388540325946963 1102 9 0.013696832102276346 1102 10-11 -0.2524448113111504 1102 12-13 -0.06712080348210492 1102 14-15 0.00931443063277726 1102 16-17 0.012322165919705697 1102 18-19 0.014077905471268082 1102 20-21 0.01636032617793859 1102 22-23 -0.017747261953573457 1102 24-25 0.02645132342134815 1102 26-27 0.08053874227626778 1102 28-29 -0.017962919878875994 1102 30-31 -0.05972222288012574 1102 32-33 -0.17381506831255678 1102 34-35 -0.05250874689096463 1102 36-37 -0.003215720872617567 1102 38-39 0.16885227765244082 1102 40-41 0.07545508356346176 1102 42-43 -0.10728538086539174 1102 44-45 -0.027241142247611094 1102 46-47 -0.11092821353852145 1102 48-49 -0.1774130516877861 1102 50-51 -0.07344706013145696 1102 52-53 -0.12411366561802595 1102 54-55 -0.028184275748394327 1102 56-57 -0.1263367367957784 1102 58-59 -0.2355668649610081 1102 60-61 -0.15674377881153845 1102 62-63 -0.21114894721705468 1102 64-65 -0.19183681980916134 1102 66-67 -0.05965991857468822 1102 68-69 -0.12751905897792426 1102 70-71 -0.3350808778753418 1102 72-73 -0.3160781749242929 1102 74-75 -0.24660424246057744 1102 76-77 -0.3119133467259765 1102 78-79 -0.30995676119571414 1102 80-81 -0.3130497850563536 1102 82-83 -0.24616522310403965 1102 84-85 -0.11181972522456718 1102 86-87 -0.20118319851330213 1102 88-89 -0.04725082859631158 1102 90-91 -0.06889552482891403 1102 92-93 -0.2501978019403488 1102 94-95 -0.35590412270464356 1102 96-97 -0.2044965089474502 1102 98-99 0.034367307421284465 1102 100-101 0.25023767382256423 1103 1 0.01814751341806442 1103 2 -0.002205335675046882 1103 3 0.03586227496771244 1103 4 -0.05881922476981316 1103 5 -0.1453558299533455 1103 6 -0.07799747689614378 1103 7 -0.021813570665699444 1103 8 0.02245743496705188 1103 9 0.027799240272649683 1103 10-11 -0.040781768472903934 1103 12-13 -0.03278394090204273 1103 14-15 0.034943982280026376 1103 16-17 0.10274755479803588 1103 18-19 -0.0013285554781603537 1103 20-21 0.02017233919621475 1103 22-23 0.06864577102312808 1103 24-25 0.10105877463490032 1103 26-27 -0.01781783131346515 1103 28-29 -0.16748007416926924 1103 30-31 -0.1539810292931918 1103 32-33 -0.03170774034884971 1103 34-35 0.02585206742028845 1103 36-37 0.08035655228857763 1103 38-39 0.057213931144382 1103 40-41 0.1578229469924466 1103 42-43 -0.02708165314135158 1103 44-45 -0.14796033554615917 1103 46-47 -0.050734540528132754 1103 48-49 -0.09893030117805068 1103 50-51 -0.1243932093044009 1103 52-53 -0.13504766444379612 1103 54-55 -0.08916342496754481 1103 56-57 0.033298610607246815 1103 58-59 0.026847329587035063 1103 60-61 0.038719367788381476 1103 62-63 0.11201814231115748 1103 64-65 0.08310442595224288 1103 66-67 0.2222257018711602 1103 68-69 0.19014761072813968 1103 70-71 0.10616828352353025 1103 72-73 0.2749610290316973 1103 74-75 0.3321743076484758 1103 76-77 0.3166732040976967 1103 78-79 0.42733486738199034 1103 80-81 0.2821236201178543 1103 82-83 0.3762968928336399 1103 84-85 0.3282298946173121 1103 86-87 0.38840799376771784 1103 88-89 0.46435111477924096 1103 90-91 0.40922365026420593 1103 92-93 0.4183233561537172 1103 94-95 0.3623557998341873 1103 96-97 0.4610940462980686 1103 98-99 0.5483792293505942 1103 100-101 0.6719062231078929 1104 1 -0.05100163686916659 1104 2 -0.09544865573889894 1104 3 0.05046218554852189 1104 4 -0.03683716084584887 1104 5 0.048935937796436235 1104 6 -0.028218336150032997 1104 7 0.015968495409239836 1104 8 0.01646343004745887 1104 9 0.09513573144519683 1104 10-11 0.006849007092604609 1104 12-13 0.13480029673790028 1104 14-15 0.1657215451308076 1104 16-17 0.1318966743570229 1104 18-19 -0.003962657376092693 1104 20-21 0.13736540628980265 1104 22-23 0.07581420942914008 1104 24-25 0.018097509122888766 1104 26-27 0.07478465122136413 1104 28-29 -0.01627760116019772 1104 30-31 -0.0530073280608363 1104 32-33 -0.057564251740579664 1104 34-35 0.014379600695136219 1104 36-37 -0.146622385036558 1104 38-39 -0.00975942900446114 1104 40-41 -0.030274851789791057 1104 42-43 0.030498390093285366 1104 44-45 0.03308586897796317 1104 46-47 0.1613681624818497 1104 48-49 0.19238681890946197 1104 50-51 0.0867990158352967 1104 52-53 0.03860401861895468 1104 54-55 0.09087051437797555 1104 56-57 0.19785127651287837 1104 58-59 0.08938691140636479 1104 60-61 0.11532403510136646 1104 62-63 0.212471153448611 1104 64-65 0.2490671341553039 1104 66-67 0.1347517632631181 1104 68-69 0.21499692710152374 1104 70-71 0.15905174512167264 1104 72-73 0.37438886912805813 1104 74-75 0.3595467201382796 1104 76-77 0.27084994066599677 1104 78-79 0.1850283746576693 1104 80-81 0.2526398699814969 1104 82-83 0.40459468639207685 1104 84-85 0.4526479400858925 1104 86-87 0.38792402392441616 1104 88-89 0.29827740079466025 1104 90-91 0.4139020254161174 1104 92-93 0.49650215458719416 1104 94-95 0.4176975977744739 1104 96-97 0.38423710010168577 1104 98-99 0.3411280551475677 1104 100-101 0.530714013332318 1105 1 -0.015342054762943036 1105 2 0.06634640612081455 1105 3 -0.02356039108639152 1105 4 -0.005759053400744563 1105 5 0.07669270048249643 1105 6 -0.035852243649650006 1105 7 -0.11049439873453082 1105 8 0.015645060271957334 1105 9 0.002128106180101952 1105 10-11 0.11427067693923476 1105 12-13 0.1002122073089069 1105 14-15 0.14942503400536822 1105 16-17 0.09735129837893908 1105 18-19 0.10111392767519334 1105 20-21 0.08411536141574061 1105 22-23 0.17950252422561164 1105 24-25 0.10005872132065718 1105 26-27 0.05104120781918198 1105 28-29 0.002665648120157016 1105 30-31 0.09920770507948617 1105 32-33 0.012784635715298975 1105 34-35 0.20837985664344671 1105 36-37 0.1511426377993459 1105 38-39 0.10448243217183517 1105 40-41 0.11992450534783927 1105 42-43 0.12854836316886065 1105 44-45 0.139744430614968 1105 46-47 -0.028376869794229265 1105 48-49 0.016249677153986397 1105 50-51 0.016172490948477503 1105 52-53 -0.10406106803783643 1105 54-55 -0.0038811326659882184 1105 56-57 -0.004308495061579265 1105 58-59 0.19257496360652482 1105 60-61 0.17450850734925893 1105 62-63 0.20609637864988173 1105 64-65 0.09434048362113145 1105 66-67 0.2866928984769501 1105 68-69 0.252144416481336 1105 70-71 0.19286068802192347 1105 72-73 0.2655072136411256 1105 74-75 0.09159776354312044 1105 76-77 0.09851562564349692 1105 78-79 0.30431374704583547 1105 80-81 0.2245295994740566 1105 82-83 0.27242364644419936 1105 84-85 0.32457581252391776 1105 86-87 0.2649819978775234 1105 88-89 0.24552595651493903 1105 90-91 0.2693066065583771 1105 92-93 0.1870299066962744 1105 94-95 0.3021472424384548 1105 96-97 0.24959424770740668 1105 98-99 0.21491587400264933 1105 100-101 0.32584310102619085 1106 1 -0.09916236101699027 1106 2 -0.027553335284014935 1106 3 -0.27297905024496316 1106 4 -0.18677198168284548 1106 5 -0.16879960108833814 1106 6 -0.10272600299447276 1106 7 -0.16833569010108107 1106 8 -0.1570951110076635 1106 9 -0.21854887722565763 1106 10-11 -0.3577145079123838 1106 12-13 -0.4209246997511187 1106 14-15 -0.4430594192795567 1106 16-17 -0.49088221534845644 1106 18-19 -0.47826668780049886 1106 20-21 -0.4177664296603183 1106 22-23 -0.2872093455363256 1106 24-25 -0.30208468236168784 1106 26-27 -0.20603255676693522 1106 28-29 -0.0823936984748812 1106 30-31 -0.11515171821563541 1106 32-33 -0.062137649164604625 1106 34-35 -0.03814644727638239 1106 36-37 -0.23604009774334855 1106 38-39 -0.30811758613579343 1106 40-41 -0.1646848834359389 1106 42-43 -0.16135274983125214 1106 44-45 -0.03611325013405775 1106 46-47 -0.0369114608661576 1106 48-49 0.02988525728046909 1106 50-51 -0.05783759633015961 1106 52-53 -0.15442723894688015 1106 54-55 -0.023546832153741093 1106 56-57 -0.13851102830503947 1106 58-59 -0.09741187409861851 1106 60-61 0.0249527163455312 1106 62-63 0.06035164596701037 1106 64-65 0.0014749291179398938 1106 66-67 -0.05703294819523563 1106 68-69 -0.02934963132666013 1106 70-71 -0.15629271361557073 1106 72-73 0.13832114138655172 1106 74-75 0.16359950276727986 1106 76-77 0.11526783861429735 1106 78-79 0.11737197266808863 1106 80-81 0.1636858210760188 1106 82-83 -0.18806104786648703 1106 84-85 -0.04625297466251865 1106 86-87 -0.07369135094839052 1106 88-89 0.052921922341695904 1106 90-91 0.15755319584089023 1106 92-93 0.13630219714966074 1106 94-95 0.017067475092900963 1106 96-97 0.20584728821228282 1106 98-99 0.32563320615386004 1106 100-101 0.3693032657062112 1107 1 -0.23444640473593026 1107 2 -0.28141876260747267 1107 3 -0.23659832319432184 1107 4 -0.2578993317102771 1107 5 -0.2096931042285135 1107 6 -0.1816309056313088 1107 7 -0.33760945099360384 1107 8 -0.14256736096372435 1107 9 -0.2779082479578605 1107 10-11 -0.23462322336597907 1107 12-13 -0.1757284917926185 1107 14-15 -0.1047194182315252 1107 16-17 -0.182569838904449 1107 18-19 -0.25417146662049817 1107 20-21 -0.20048878639101986 1107 22-23 -0.25805924381639045 1107 24-25 -0.27957844398689957 1107 26-27 -0.2130461548240774 1107 28-29 -0.3099279208471515 1107 30-31 -0.24097323368741286 1107 32-33 -0.3488869747801715 1107 34-35 -0.1933772451159399 1107 36-37 -0.14477776417150778 1107 38-39 -0.03143257571801428 1107 40-41 -0.06196207224642336 1107 42-43 -0.11744650932329392 1107 44-45 -0.13309977079268975 1107 46-47 -0.1524330845269546 1107 48-49 -0.16196476574389607 1107 50-51 -0.2952335751115669 1107 52-53 -0.08049430130186863 1107 54-55 0.004082919317568212 1107 56-57 -0.02574249763068792 1107 58-59 -0.15919318573169505 1107 60-61 -0.12568484764388899 1107 62-63 -0.013337877968830014 1107 64-65 0.11495584718040419 1107 66-67 -0.07830027617677615 1107 68-69 -0.09167953967901354 1107 70-71 -0.016555158947184623 1107 72-73 0.017608624153051977 1107 74-75 -0.06075684674123494 1107 76-77 -0.18196585199477155 1107 78-79 -0.034169129654237906 1107 80-81 -2.383566433508122E-4 1107 82-83 0.04234238635824994 1107 84-85 0.13730275752397247 1107 86-87 0.139669042779893 1107 88-89 0.07447481740668849 1107 90-91 0.05278063624997387 1107 92-93 0.016929127730147542 1107 94-95 0.03422422304123174 1107 96-97 0.09461742525944317 1107 98-99 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42-43 -0.44498943621766784 2316 44-45 -0.387467357958478 2316 46-47 -0.4118132475137486 2316 48-49 -0.2960764048249658 2316 50-51 -0.19406242343589497 2316 52-53 -0.4077678296198144 2316 54-55 -0.574731260455124 2316 56-57 -0.6035949283729707 2316 58-59 -0.7855214217865338 2316 60-61 -0.6392299333551392 2316 62-63 -0.5862710310983985 2316 64-65 -0.7507374945670406 2316 66-67 -0.7913060464117976 2316 68-69 -0.45440405469030765 2316 70-71 -0.6118604449385927 2316 72-73 -1.1516081503464726 2316 74-75 -1.1205260114242357 2316 76-77 -0.813659512511105 2316 78-79 -0.9679758650073929 2316 80-81 -0.9105300560831715 2316 82-83 -0.9522242888175576 2316 84-85 -0.9252399555620094 2316 86-87 -1.290209319681054 2316 88-89 -1.211204425944839 2316 90-91 -1.246452893052787 2316 92-93 -1.299140452000298 2316 94-95 -1.3944350428314536 2316 96-97 -1.7648298606049693 2316 98-99 -1.9089530787040268 2316 100-101 -2.1058787982565796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10850.0 3 2278.0 4 2016.0 5 2260.0 6 2578.0 7 3265.0 8 4034.0 9 4881.0 10 6196.0 11 8038.0 12 9975.0 13 12351.0 14 14579.0 15 17457.0 16 21383.0 17 27487.0 18 23692.0 19 16856.0 20 16753.0 21 17783.0 22 19414.0 23 21489.0 24 24354.0 25 27318.0 26 31711.0 27 37046.0 28 44776.0 29 53441.0 30 65085.0 31 80249.0 32 100999.0 33 130885.0 34 174836.0 35 242934.0 36 354386.0 37 504669.0 38 431948.0 39 59640.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.76278017582577 19.508572889108454 15.337164699801056 22.39148223526472 2 14.934486226262484 20.43219756295397 40.92338974622398 23.709926464559565 3 18.890398635355297 25.08652016744584 30.089683516377907 25.933397680820953 4 12.379085909497055 17.11291188015619 38.286249057570835 32.22175315277592 5 13.457949056821766 37.51981358736415 34.4899600353699 14.532277320444189 6 30.87076488437396 36.70844449084873 18.115718641196306 14.305071983581014 7 27.67824614301154 33.14929338721405 21.180167587120454 17.992292882653956 8 25.92325837515672 34.79488439978202 20.21096515797617 19.07089206708509 9 26.006237253413573 17.13255535333766 20.988083370677593 35.873124022571176 10-11 24.255169301877473 27.109642249553346 27.750031250571656 20.885157197997525 12-13 25.37961822472362 24.4795827057812 28.26151164350567 21.8792874259895 14-15 22.5559424745193 25.817746815793253 26.874449643866477 24.751861065820975 16-17 21.453332492403526 29.15868212072081 27.386898910646572 22.00108647622909 18-19 21.342613478755215 27.583125798260667 30.024831812764248 21.04942891021987 20-21 22.331233757821014 26.558582348323768 30.08116326390635 21.029020629948864 22-23 21.88725294070577 26.54633302468783 29.97283157481605 21.59358245979035 24-25 21.695792475823637 26.71648214150661 30.081769947986746 21.505955434683006 26-27 21.475255879868737 26.95034866424602 29.978456533622538 21.595938922262707 28-29 21.36788989112653 27.132303879334163 29.98097734238383 21.518828887155475 30-31 21.56913444772413 26.801227637506624 30.01675454242836 21.612883372340885 32-33 21.33412118316956 26.902982237746325 29.95151141216748 21.811385166916633 34-35 21.810855147211527 26.963992096894962 29.762220947801023 21.46293180809249 36-37 21.775944639547205 26.64640934186684 29.825725913403843 21.75192010518212 38-39 21.46327803082691 27.10013316035822 29.841312477258914 21.595276331555958 40-41 21.798560972910177 26.975576088047777 29.69831257509419 21.527550363947853 42-43 21.479490092713878 26.940403881074467 29.539242423896056 22.0408636023156 44-45 21.681152384313048 27.101997794370387 29.39576875795006 21.821081063366503 46-47 21.855464385252017 27.33355725296714 28.860999061328386 21.94997930045246 48-49 21.83625249429216 27.363811600418636 28.813792480608207 21.986143424681 50-51 21.898244020148912 27.56379231893149 28.64757006503657 21.890393595883022 52-53 22.025593756943536 27.690128563332483 28.339748224264945 21.944529455459037 54-55 21.838407159443744 27.545542097594755 28.525708575546776 22.09034216741473 56-57 21.962804552938202 27.832580675824026 28.322787492658996 21.88182727857878 58-59 22.071770757781692 27.47893823405721 28.48519086249246 21.964100145668645 60-61 21.912331701084096 27.536972283383154 28.719986300459198 21.830709715073553 62-63 21.730504862176893 27.5771556641394 28.856364107722776 21.835975365960934 64-65 21.762499792853475 27.501687172744205 28.67206980999854 22.063743224403783 66-67 21.726509967067596 27.63416854678118 28.39304074096864 22.246280745182588 68-69 21.744921954244013 27.581672081153492 28.598071737902647 22.07533422669985 70-71 21.84202325083455 27.437855412634875 28.549497364717464 22.170623971813104 72-73 21.85961495631293 27.368447725359307 28.553840901370542 22.218096416957227 74-75 21.687943681236256 27.859633615619295 28.22256998650323 22.22985271664122 76-77 21.775045500382273 27.631463383361364 28.265944376080355 22.327546740176004 78-79 21.763092012300287 27.60494747268185 28.277191981761042 22.354768533256824 80-81 21.781025479550014 27.837854672901734 28.11267241827615 22.2684474292721 82-83 21.855906852014638 27.67613181942709 28.279396708438924 22.188564620119347 84-85 21.86631298602992 27.666043039779165 28.150258650870658 22.317385323320256 86-87 21.784101948682782 27.594795893098656 28.33116178067318 22.289940377545385 88-89 21.907402482505944 27.407770918758857 28.136851778895576 22.547974819839624 90-91 21.895026037182042 27.35816707991899 28.334694754616514 22.41211212828246 92-93 21.882328600989958 27.26103355110927 28.401566279795915 22.455071568104863 94-95 21.74434859539292 27.31012153029799 28.395824451071455 22.549705423237633 96-97 21.607237933047784 27.392364891186222 28.391019065389226 22.609378110376767 98-99 21.60941529513002 27.41533361236205 28.21683312818944 22.75841796431849 100-101 21.70861405985599 27.160829154974753 28.52037345040102 22.610183334768237 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3508.0 1 1998.0 2 475.5 3 442.0 4 399.0 5 414.0 6 664.5 7 1269.5 8 2136.0 9 2759.0 10 2770.0 11 2665.0 12 2758.0 13 2977.0 14 3227.5 15 3370.5 16 3542.5 17 3767.0 18 3997.0 19 4452.5 20 5030.5 21 5661.5 22 6640.0 23 8055.0 24 9832.0 25 12413.5 26 15945.5 27 19868.0 28 23221.5 29 27199.5 30 32286.5 31 37664.5 32 43808.0 33 49831.5 34 56556.0 35 63194.5 36 69942.5 37 76376.0 38 80456.5 39 83052.5 40 85041.5 41 89314.0 42 93192.0 43 96474.5 44 100897.0 45 103085.0 46 103581.0 47 105481.0 48 108816.0 49 106807.0 50 99491.0 51 91877.0 52 85662.5 53 82021.5 54 78585.5 55 71030.0 56 62203.5 57 54613.5 58 48208.5 59 42207.5 60 34728.0 61 28096.0 62 22249.5 63 16440.0 64 12126.0 65 9376.0 66 6927.5 67 5171.5 68 3905.5 69 2732.5 70 1862.0 71 1260.0 72 892.5 73 695.0 74 557.0 75 444.5 76 315.0 77 207.0 78 127.5 79 84.5 80 60.5 81 41.5 82 27.0 83 16.0 84 13.0 85 13.5 86 14.5 87 10.0 88 6.0 89 6.5 90 7.0 91 5.0 92 1.5 93 2.5 94 5.5 95 4.5 96 2.0 97 2.5 98 4.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3255249427165838 2 0.2902385104258479 3 0.2922157674076564 4 0.29251996078947307 5 0.27898335529863044 6 0.21396201993531325 7 0.22198512038072846 8 0.21962762167164912 9 0.21574915605348635 10-11 0.22639592441707043 12-13 0.22639592441707043 14-15 0.20795420064443365 16-17 0.22154784239436695 18-19 0.20723174136261904 20-21 0.21164254539896102 22-23 0.21050182021714847 24-25 0.20101478912174048 26-27 0.21584421648530405 28-29 0.21548298684439673 30-31 0.22211820498527327 32-33 0.2238292927579921 34-35 0.21373387489895074 36-37 0.20934208294897233 38-39 0.1996649309899289 40-41 0.2009767649490134 42-43 0.2004634386171977 44-45 0.2171940746171156 46-47 0.20732680179443674 48-49 0.21538792641257898 50-51 0.22221326541709097 52-53 0.23054055924432282 54-55 0.2287153989534227 56-57 0.19375217213086704 58-59 0.18137530390820053 60-61 0.19013987572179386 62-63 0.19017789989452097 64-65 0.18831471543089373 66-67 0.20105281329446753 68-69 0.19025394823997513 70-71 0.1954442478172224 72-73 0.20922801043079106 74-75 0.19785878278539235 76-77 0.20827740611261392 78-79 0.2058818832308075 80-81 0.19299168867632532 82-83 0.20673742711716692 84-85 0.18780138909907806 86-87 0.19225021730814715 88-89 0.20152811545355612 90-91 0.19764964983539335 92-93 0.20316315488082085 94-95 0.20126194624446653 96-97 0.19481684896722545 98-99 0.20249773185809683 100-101 0.1852537695263633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2629906.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.23462709820002 #Duplication Level Percentage of deduplicated Percentage of total 1 77.72900726876534 44.48790745741063 2 12.72402363422126 14.56509495786675 3 4.07787007673216 7.0018611959002035 4 1.8167254952238687 4.159184250357236 5 0.9666853622200076 2.7663938113975273 6 0.5884507440240614 2.0207855339925302 7 0.3834502655114949 1.5362643070079343 8 0.285441999963523 1.306973314086133 9 0.18960815598652545 0.9766936892399514 >10 1.1104940133993986 11.852124479626264 >50 0.08508574658514272 3.337803826797835 >100 0.04143569101701736 4.175392652650676 >500 0.0011256264598238361 0.4302061475502055 >1k 3.9727992698993875E-4 0.46336597657037765 >5k 1.3242664232997957E-4 0.5061137333031319 >10k+ 6.621332116498978E-5 0.4138346662426208 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10920 0.4152239661797798 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8120 0.30875628254393883 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5235 0.19905654422629557 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3900 0.14829427363563563 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 7.604834545417213E-5 0.0 0.0 0.0 1.9012086363543033E-5 16-17 7.604834545417213E-5 0.0 0.0 0.0 7.604834545417213E-5 18-19 7.604834545417213E-5 0.0 0.0 0.0 7.604834545417213E-5 20-21 7.604834545417213E-5 3.8024172727086066E-5 0.0 1.9012086363543033E-5 7.604834545417213E-5 22-23 7.604834545417213E-5 3.8024172727086066E-5 0.0 5.70362590906291E-5 7.604834545417213E-5 24-25 1.1407251818125818E-4 3.8024172727086066E-5 0.0 1.1407251818125818E-4 7.604834545417213E-5 26-27 1.1407251818125818E-4 3.8024172727086066E-5 0.0 2.851812954531455E-4 7.604834545417213E-5 28-29 1.9012086363543032E-4 3.8024172727086066E-5 0.0 0.0010266526636313238 7.604834545417213E-5 30-31 1.9012086363543032E-4 7.604834545417213E-5 0.0 0.0023194745363522497 7.604834545417213E-5 32-33 1.9012086363543032E-4 1.330846045448012E-4 0.0 0.004087598568161752 7.604834545417213E-5 34-35 1.9012086363543032E-4 1.5209669090834426E-4 0.0 0.006045843463606684 7.604834545417213E-5 36-37 1.9012086363543032E-4 1.5209669090834426E-4 0.0 0.008479390518140192 7.604834545417213E-5 38-39 1.9012086363543032E-4 1.5209669090834426E-4 0.0 0.01085590131358307 7.604834545417213E-5 40-41 1.9012086363543032E-4 1.5209669090834426E-4 0.0 0.013878823045386414 7.604834545417213E-5 42-43 2.0913294999897334E-4 1.5209669090834426E-4 0.0 0.018517772118090914 7.604834545417213E-5 44-45 2.2814503636251637E-4 1.5209669090834426E-4 0.0 0.026065570404417496 7.604834545417213E-5 46-47 2.2814503636251637E-4 1.5209669090834426E-4 0.0 0.044374209572509433 7.604834545417213E-5 48-49 2.2814503636251637E-4 1.5209669090834426E-4 0.0 0.0793944726541557 7.604834545417213E-5 50-51 2.2814503636251637E-4 1.5209669090834426E-4 0.0 0.10783655385401608 7.604834545417213E-5 52-53 2.2814503636251637E-4 1.9012086363543032E-4 0.0 0.13641171965842125 7.604834545417213E-5 54-55 2.2814503636251637E-4 2.0913294999897334E-4 0.0 0.17671734274913248 7.604834545417213E-5 56-57 2.2814503636251637E-4 2.2814503636251637E-4 0.0 0.20755494683079928 7.604834545417213E-5 58-59 2.2814503636251637E-4 2.661692090896024E-4 0.0 0.2518150838851274 7.604834545417213E-5 60-61 2.2814503636251637E-4 2.661692090896024E-4 0.0 0.3245363142256795 7.604834545417213E-5 62-63 2.2814503636251637E-4 2.851812954531455E-4 0.0 0.3767815275526958 1.1407251818125818E-4 64-65 2.2814503636251637E-4 3.0419338181668853E-4 0.0 0.4445596154387267 1.1407251818125818E-4 66-67 2.2814503636251637E-4 3.0419338181668853E-4 0.0 0.5607424752063381 1.1407251818125818E-4 68-69 2.2814503636251637E-4 3.0419338181668853E-4 0.0 0.688104441755713 1.1407251818125818E-4 70-71 2.4715712272605937E-4 3.0419338181668853E-4 0.0 0.8043823619551421 1.1407251818125818E-4 72-73 2.851812954531455E-4 3.0419338181668853E-4 0.0 0.9337596096590525 2.2814503636251637E-4 74-75 3.0419338181668853E-4 3.0419338181668853E-4 0.0 1.0542011767720976 2.2814503636251637E-4 76-77 3.0419338181668853E-4 3.0419338181668853E-4 0.0 1.164851519407918 2.2814503636251637E-4 78-79 3.0419338181668853E-4 3.0419338181668853E-4 0.0 1.303810858639054 2.2814503636251637E-4 80-81 3.0419338181668853E-4 3.0419338181668853E-4 0.0 1.468075284820066 2.2814503636251637E-4 82-83 3.0419338181668853E-4 3.0419338181668853E-4 0.0 1.6758203525145006 2.2814503636251637E-4 84-85 3.232054681802316E-4 3.0419338181668853E-4 0.0 1.9106386311906205 2.2814503636251637E-4 86-87 3.422175545437746E-4 3.0419338181668853E-4 0.0 2.2712028490752143 2.2814503636251637E-4 88-89 3.422175545437746E-4 3.0419338181668853E-4 0.0 2.7373792067092895 2.2814503636251637E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8195 0.0 35.683895 1 GTATCAA 12945 0.0 34.34438 1 ATCAACG 16025 0.0 27.495531 3 TATCAAC 16170 0.0 27.425865 2 TCAACGC 16075 0.0 27.38044 4 CAACGCA 16250 0.0 27.082993 5 AACGCAG 16590 0.0 26.572575 6 GTACATG 11365 0.0 24.350039 1 ACGCAGA 18580 0.0 23.47086 7 CGCAGAG 18615 0.0 23.3748 8 TACATGG 11340 0.0 23.179325 2 ACATGGG 11465 0.0 22.014097 3 AGAGTAC 15710 0.0 21.48219 10-11 GAGTACT 9710 0.0 21.474728 12-13 GCAGAGT 19780 0.0 21.27599 9 GTACTTT 9885 0.0 21.07012 14-15 AGTACTT 10060 0.0 20.892847 12-13 CATGGGG 5390 0.0 20.811514 4 GCACCGT 385 0.0 19.742006 6 TATCCCG 195 1.0284439E-5 19.498196 5 >>END_MODULE