FastQCFastQC Report
Thu 26 May 2016
SRR937493_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937493_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2629906
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126440.4807776399612762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105390.40073675637076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67860.258032036126006No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.1064296594631139No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27940.10623953859947846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93800.034.655311
GTATCAA145650.034.195371
ATCAACG179550.027.5412483
TCAACGC181150.027.297994
TATCAAC182150.027.2524412
CAACGCA184150.026.8274825
AACGCAG189850.026.0440716
ACGCAGA214150.022.9570357
CGCAGAG214350.022.8248168
GAGTACT119550.022.30969812-13
GTACATG110350.021.8780141
GTACTTT122150.021.63916214-15
AGTACTT121100.021.25928712-13
TACATGG110950.021.1931822
GCAGAGT227800.021.0809889
AGAGTAC182050.020.7820610-11
GCACCGT3553.6379788E-1220.0692756
ACATGGG110000.019.9511343
TACTTTT137350.018.63926914-15
ACTTTTT143350.018.50557116-17
Produced by FastQC (version 0.11.5)