##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937493_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2629906 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.101098670446774 33.0 31.0 34.0 31.0 34.0 2 32.32884825541293 34.0 31.0 34.0 31.0 34.0 3 32.40492816093047 34.0 31.0 34.0 31.0 34.0 4 36.03139351748694 37.0 35.0 37.0 35.0 37.0 5 35.91111393334971 37.0 35.0 37.0 35.0 37.0 6 35.98746343025188 37.0 35.0 37.0 35.0 37.0 7 35.93922558448857 37.0 35.0 37.0 35.0 37.0 8 35.91879329527367 37.0 35.0 37.0 35.0 37.0 9 37.60482846154957 39.0 37.0 39.0 35.0 39.0 10-11 37.529515503595945 39.0 37.0 39.0 35.0 39.0 12-13 37.449351992048385 39.0 37.0 39.0 34.5 39.0 14-15 38.646284886227875 40.0 38.0 41.0 34.0 41.0 16-17 38.62306238321826 40.0 38.0 41.0 34.0 41.0 18-19 38.63314126056217 40.0 38.0 41.0 34.0 41.0 20-21 38.53139884087112 40.0 38.0 41.0 34.0 41.0 22-23 38.4071128397745 40.0 38.0 41.0 34.0 41.0 24-25 38.26552412899929 40.0 38.0 41.0 34.0 41.0 26-27 38.21755986715875 40.0 38.0 41.0 34.0 41.0 28-29 38.122812754524304 40.0 38.0 41.0 33.0 41.0 30-31 38.20495333293282 40.0 38.0 41.0 34.0 41.0 32-33 38.13500862768479 40.0 37.0 41.0 34.0 41.0 34-35 38.0213477211733 40.0 37.0 41.0 33.0 41.0 36-37 37.86265459678027 40.0 37.0 41.0 33.0 41.0 38-39 37.73535498987416 40.0 37.0 41.0 33.0 41.0 40-41 37.57701777173785 40.0 37.0 41.0 33.0 41.0 42-43 37.366437241483155 40.0 37.0 41.0 32.5 41.0 44-45 37.079156441332884 40.0 36.5 41.0 31.5 41.0 46-47 36.732382830412945 40.0 36.0 41.0 31.0 41.0 48-49 36.42041350527357 39.0 36.0 41.0 30.0 41.0 50-51 36.20010962368997 39.0 36.0 41.0 30.0 41.0 52-53 35.972216687592635 39.0 35.0 40.5 29.0 41.0 54-55 35.745681214461655 39.0 35.0 40.0 28.5 41.0 56-57 35.50371153949989 39.0 35.0 40.0 28.0 41.0 58-59 35.26680383253242 38.0 35.0 40.0 28.0 41.0 60-61 34.942445281314235 38.0 34.0 40.0 27.0 41.0 62-63 34.58088616095024 38.0 34.0 40.0 26.0 41.0 64-65 34.23948042249419 37.0 34.0 40.0 26.0 41.0 66-67 33.80934584734207 36.5 33.0 39.0 25.5 41.0 68-69 33.401905809561256 36.0 33.0 39.0 24.5 40.0 70-71 32.99385871586284 35.5 32.5 39.0 23.5 40.0 72-73 32.535713253629595 35.0 32.0 38.0 22.5 40.0 74-75 32.08266626259646 35.0 31.5 37.0 21.5 39.0 76-77 30.743935524691757 34.0 30.0 36.0 20.5 38.5 78-79 31.381482075785218 34.5 31.0 36.0 20.5 39.0 80-81 31.233741243983623 35.0 31.0 36.0 20.0 37.5 82-83 30.9017795693078 34.0 31.0 35.5 19.5 37.0 84-85 30.52750744703423 34.0 31.0 35.0 18.0 37.0 86-87 30.147667255027365 34.0 30.5 35.0 15.0 36.0 88-89 29.746844754147105 34.0 30.0 35.0 8.5 36.0 90-91 29.291440074284026 34.0 29.5 35.0 4.5 35.5 92-93 28.909132683829764 34.0 29.0 35.0 2.0 35.0 94-95 28.47894582544015 34.0 29.0 35.0 2.0 35.0 96-97 27.98475344746162 33.5 29.0 35.0 2.0 35.0 98-99 27.46811996322302 33.0 28.0 35.0 2.0 35.0 100-101 25.556474451938584 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13452965994150645 1101 2 0.1032391121516909 1101 3 0.014911526676911535 1101 4 0.030501616575946855 1101 5 -0.023862194999090036 1101 6 -0.007858355553800322 1101 7 0.01964674580401038 1101 8 0.001110129128235826 1101 9 0.0812175292042312 1101 10-11 -0.0109832047973768 1101 12-13 0.04149272668303894 1101 14-15 0.06278443493307861 1101 16-17 -0.02016956637339007 1101 18-19 0.04350133180648186 1101 20-21 0.08417729501969973 1101 22-23 0.07900250102699857 1101 24-25 0.12257530841882414 1101 26-27 0.002108204045512707 1101 28-29 -0.039261265192379824 1101 30-31 0.07453900303677585 1101 32-33 -0.035642535007575304 1101 34-35 -0.045952864309427355 1101 36-37 -0.13622120377011981 1101 38-39 -0.07313191354320026 1101 40-41 -0.026570755966758952 1101 42-43 -0.028278226546248675 1101 44-45 -0.06762569739753133 1101 46-47 0.007311232030652093 1101 48-49 0.04245751161785449 1101 50-51 0.04069884650114375 1101 52-53 0.024416342993738738 1101 54-55 -0.06980161473953217 1101 56-57 0.01613099497360082 1101 58-59 -0.020251927265015013 1101 60-61 0.037203105167854744 1101 62-63 -0.04473540977805612 1101 64-65 -0.018497173282376878 1101 66-67 -0.076356569383492 1101 68-69 0.020396778990516395 1101 70-71 -0.22612112022819275 1101 72-73 -0.1692539650575995 1101 74-75 -0.05748886745002579 1101 76-77 -0.04463701270571363 1101 78-79 -0.08179301177176868 1101 80-81 -0.03388244356043657 1101 82-83 -0.14286057973782462 1101 84-85 -0.03442127664239081 1101 86-87 0.0220041835139817 1101 88-89 -0.017856231398223343 1101 90-91 -0.07453292978181736 1101 92-93 -0.1673439740273608 1101 94-95 -0.25114241690986105 1101 96-97 -0.23878522350046794 1101 98-99 -0.1597876080754297 1101 100-101 0.198985112809261 1102 1 0.061338286668672026 1102 2 0.11040870720727014 1102 3 0.1069805798213892 1102 4 0.04645708512864388 1102 5 -0.07986590532480164 1102 6 0.028574109105129253 1102 7 0.003688214630614084 1102 8 -0.043135316519197886 1102 9 0.026954408308931477 1102 10-11 0.003304706049270578 1102 12-13 -0.057549403355523054 1102 14-15 -0.06853779579817143 1102 16-17 0.011604887789076201 1102 18-19 -0.008100803609721652 1102 20-21 -0.0370338505323744 1102 22-23 -0.0060285319588473385 1102 24-25 -0.013424407935382021 1102 26-27 0.002329301701337272 1102 28-29 -0.10484819053479555 1102 30-31 0.036639464534445665 1102 32-33 -0.04168909061157677 1102 34-35 -0.0019512111224884165 1102 36-37 0.011660970458123643 1102 38-39 -0.1614280928374967 1102 40-41 -0.0030360864722212 1102 42-43 0.10094548077652377 1102 44-45 0.11030447673523014 1102 46-47 0.15520082722444073 1102 48-49 0.2370794397307634 1102 50-51 0.2189895067958858 1102 52-53 0.22771216262642469 1102 54-55 0.18359467455780987 1102 56-57 0.09529506757436224 1102 58-59 0.12987872099284203 1102 60-61 0.12534674239272903 1102 62-63 0.0375057720731391 1102 64-65 0.18277452891906876 1102 66-67 0.14956738401692604 1102 68-69 0.17019438687957944 1102 70-71 0.11480836316580678 1102 72-73 0.14348974887415977 1102 74-75 0.09759871093735484 1102 76-77 0.012063443828942866 1102 78-79 0.002053690084149906 1102 80-81 -0.01291448579758736 1102 82-83 0.06553768377194658 1102 84-85 -0.034827309471172185 1102 86-87 -0.08576382406749516 1102 88-89 -0.14889783969186254 1102 90-91 0.036235268153316014 1102 92-93 0.09738393332632356 1102 94-95 0.005316375293052289 1102 96-97 -0.15254952044211834 1102 98-99 -0.009402762299583145 1102 100-101 0.2594627622745733 1103 1 0.07033753356969186 1103 2 0.07238431780393739 1103 3 0.047310351556518526 1103 4 0.019694639561414817 1103 5 -0.0530624279949663 1103 6 -0.013486306649397761 1103 7 0.03358831503866355 1103 8 0.023201761806028287 1103 9 0.11111974884860132 1103 10-11 0.04875924447902946 1103 12-13 0.051857039357571466 1103 14-15 0.026114714627070157 1103 16-17 0.009856787884231721 1103 18-19 -0.012137177687733924 1103 20-21 0.0124868505714133 1103 22-23 0.03498874202684732 1103 24-25 -0.012067075683042106 1103 26-27 0.0974660796524276 1103 28-29 -0.07776780676186945 1103 30-31 -0.13761771760809438 1103 32-33 -0.05043820603322757 1103 34-35 0.042693085222964555 1103 36-37 -0.07681529378766072 1103 38-39 0.06076612664487868 1103 40-41 0.05939826077580079 1103 42-43 0.10053199497296816 1103 44-45 0.0786517173376069 1103 46-47 0.12574531485054763 1103 48-49 0.2380251793588073 1103 50-51 0.15363962486072325 1103 52-53 0.18785849842820568 1103 54-55 0.1086425874474628 1103 56-57 0.050637712155690906 1103 58-59 0.028243508662356476 1103 60-61 -0.012463416391689464 1103 62-63 -0.10428060405259743 1103 64-65 -0.0678229043625862 1103 66-67 0.1196473472722488 1103 68-69 0.21149011276082064 1103 70-71 0.15352171527226943 1103 72-73 0.22279630281345675 1103 74-75 0.18780592056577206 1103 76-77 0.21063267940334285 1103 78-79 0.1957676549625056 1103 80-81 0.12149791849834202 1103 82-83 0.013194947854376693 1103 84-85 0.16151098821604393 1103 86-87 0.1745984732599375 1103 88-89 0.08546009513239028 1103 90-91 0.17112027180908385 1103 92-93 0.16586239056913854 1103 94-95 0.14584680084729484 1103 96-97 0.17571091673238115 1103 98-99 0.26836017054880656 1103 100-101 0.46939173018897407 1104 1 0.09613404806496817 1104 2 0.045562862729404685 1104 3 -0.004008274214953644 1104 4 -0.03418042247123054 1104 5 -0.049850865571308134 1104 6 -0.024681681434110203 1104 7 -0.007103101180014448 1104 8 -0.04765418879524219 1104 9 0.020595878380440524 1104 10-11 -0.04305613408525488 1104 12-13 -8.721664556006203E-4 1104 14-15 -0.06079743485969402 1104 16-17 -0.09792260483348514 1104 18-19 -0.12925534468651279 1104 20-21 -0.2384568906230342 1104 22-23 -0.09062609172567448 1104 24-25 -0.07141514525793013 1104 26-27 -0.14249899769824026 1104 28-29 -0.05688082063412736 1104 30-31 -0.012451516218256131 1104 32-33 -0.07387748010918926 1104 34-35 -0.02619748689084389 1104 36-37 -0.18596700988327086 1104 38-39 -0.15122088452637428 1104 40-41 -0.03551360293829475 1104 42-43 0.0177905900469284 1104 44-45 0.06293737016683565 1104 46-47 0.09143246752536527 1104 48-49 0.09589053433916206 1104 50-51 0.10811557770516345 1104 52-53 0.18566457862585395 1104 54-55 0.17509249195944676 1104 56-57 0.07791502073025214 1104 58-59 0.19853866881027926 1104 60-61 0.21209505715206234 1104 62-63 0.1025941491611988 1104 64-65 -0.0860594258024534 1104 66-67 0.09456180752296461 1104 68-69 0.07842145203597539 1104 70-71 0.053154973679902184 1104 72-73 -0.025796881354736456 1104 74-75 -0.14630043295959894 1104 76-77 -0.01552658201258339 1104 78-79 -0.10742662282389048 1104 80-81 -0.04436653614446584 1104 82-83 0.05059759693629573 1104 84-85 0.10140577470852108 1104 86-87 -0.020677827428222884 1104 88-89 -0.08237573000546305 1104 90-91 -0.003976560569881826 1104 92-93 -0.052606974139781215 1104 94-95 -0.05444842075712231 1104 96-97 0.04006016166260551 1104 98-99 0.11511972357576994 1104 100-101 0.592880399917199 1105 1 -0.05594694461557026 1105 2 0.07010381778221841 1105 3 0.05238129240141376 1105 4 0.026284871603401427 1105 5 -0.09591341667786679 1105 6 -0.007838288556790474 1105 7 0.008159727813279005 1105 8 -0.01410734360740662 1105 9 0.0017595789275759444 1105 10-11 0.027320010203105483 1105 12-13 -0.010424273541715934 1105 14-15 -0.0267642047920873 1105 16-17 -0.019141467226369002 1105 18-19 0.008385759281679839 1105 20-21 -0.01288208723786255 1105 22-23 0.047607349748595595 1105 24-25 -0.026820474301082697 1105 26-27 -0.015626004565632456 1105 28-29 -0.02233910106039616 1105 30-31 0.011414250529739434 1105 32-33 -0.019886597852057264 1105 34-35 -0.08594404820874502 1105 36-37 -0.044771216077876375 1105 38-39 -0.14133662634433364 1105 40-41 -0.0669856879530144 1105 42-43 -0.21117586078108275 1105 44-45 -0.030806771276189693 1105 46-47 -0.07523068123710885 1105 48-49 -0.01715551641173363 1105 50-51 -0.05714816538921497 1105 52-53 0.14238754283962152 1105 54-55 0.04797493523531671 1105 56-57 0.06926338631750895 1105 58-59 0.09928349499811162 1105 60-61 0.07605080468731273 1105 62-63 0.06667388204423474 1105 64-65 -0.08029917623623817 1105 66-67 -0.16217977111300286 1105 68-69 0.007397043874881604 1105 70-71 0.002577427991461434 1105 72-73 0.05020306213720005 1105 74-75 0.00959510356788229 1105 76-77 -0.023935564136092324 1105 78-79 0.10742004087333257 1105 80-81 -0.03486171301472041 1105 82-83 0.07669343794253791 1105 84-85 0.023905791328541426 1105 86-87 0.08623094860703873 1105 88-89 0.1636771814896072 1105 90-91 0.12291729068758883 1105 92-93 0.01738076028560087 1105 94-95 -0.03249944089393608 1105 96-97 -0.04396076959034545 1105 98-99 -0.014435325253849385 1105 100-101 0.29207524305745025 1106 1 -0.2571487330045521 1106 2 -0.13588102158684734 1106 3 -0.14411754085674744 1106 4 -0.02876146218442699 1106 5 -0.14020116157000473 1106 6 -0.18144364265057078 1106 7 -0.2110185035374954 1106 8 -0.12956772495223134 1106 9 -0.3237310358320613 1106 10-11 -0.2664241348641312 1106 12-13 -0.42069214617380624 1106 14-15 -0.3141630145751435 1106 16-17 -0.24705296057472026 1106 18-19 -0.21916890152162694 1106 20-21 -0.1483114803475516 1106 22-23 -0.2363768669398425 1106 24-25 -0.20752030901572738 1106 26-27 -0.205082539464172 1106 28-29 -0.19477882062896157 1106 30-31 -0.082975155188592 1106 32-33 -0.09383845877022878 1106 34-35 -0.3173839113592649 1106 36-37 -0.2152950484686471 1106 38-39 -0.17203806666702093 1106 40-41 -0.22279836738433545 1106 42-43 -0.40681297158722884 1106 44-45 -0.3746115142054194 1106 46-47 -0.2454009107541637 1106 48-49 -0.22970350932786232 1106 50-51 -0.23798590707014 1106 52-53 -0.13488813895322238 1106 54-55 -0.1432332349842369 1106 56-57 -0.057253682587806054 1106 58-59 -0.21433018795667635 1106 60-61 -0.1438531338138418 1106 62-63 0.016882085114161782 1106 64-65 -0.1324675498918637 1106 66-67 -0.26459274162074564 1106 68-69 -0.009534231302716023 1106 70-71 -0.030143479682756436 1106 72-73 -0.03673789098125724 1106 74-75 -0.22282602601243795 1106 76-77 -0.15039523716785297 1106 78-79 0.01768906695948047 1106 80-81 -0.05541192720652077 1106 82-83 -0.1606737026061147 1106 84-85 -0.3915326467064304 1106 86-87 -0.20161277147236234 1106 88-89 -0.1908186502042888 1106 90-91 -0.34134981014533494 1106 92-93 -0.34431797670989894 1106 94-95 -0.8324844644465443 1106 96-97 -0.4825835879012139 1106 98-99 -0.1982588058134418 1106 100-101 0.25698546373074294 1107 1 -0.15234811753746413 1107 2 -0.15765154677893634 1107 3 -0.14805909003266748 1107 4 -0.05321672604646466 1107 5 -0.17636671628104494 1107 6 -0.14381027869536922 1107 7 -0.07548144682613156 1107 8 -0.18254955448287546 1107 9 -0.0999029251425867 1107 10-11 -0.15805907829560084 1107 12-13 -0.22127867629259867 1107 14-15 -0.3037682892653635 1107 16-17 -0.3558233202139576 1107 18-19 -0.25975235245004313 1107 20-21 -0.22934570805275456 1107 22-23 -0.25672424042290487 1107 24-25 -0.27225643770362495 1107 26-27 -0.329333992457137 1107 28-29 -0.3368388917378553 1107 30-31 -0.3511328023161582 1107 32-33 -0.32614431326643256 1107 34-35 -0.28231350965940294 1107 36-37 -0.5063605735471128 1107 38-39 -0.4351793231343706 1107 40-41 -0.3544237857299066 1107 42-43 -0.3258682908190451 1107 44-45 -0.23123601667735016 1107 46-47 -0.4363427117373746 1107 48-49 -0.4871134372126633 1107 50-51 -0.5408341117954976 1107 52-53 -0.5366414689074901 1107 54-55 -0.617485592257502 1107 56-57 -0.3772789004150212 1107 58-59 -0.37773141341880034 1107 60-61 -0.29059941733771666 1107 62-63 -0.489075823651838 1107 64-65 -0.5172732757020171 1107 66-67 -0.35511620862217086 1107 68-69 -0.44594366345641845 1107 70-71 -0.47038409244164114 1107 72-73 -0.4095776232957462 1107 74-75 -0.526383371991475 1107 76-77 -0.4372293796388398 1107 78-79 -0.43606251379205574 1107 80-81 -0.4649083543718433 1107 82-83 -0.4139858495020441 1107 84-85 -0.3169857259199915 1107 86-87 -0.36258458992441334 1107 88-89 -0.39726027937423325 1107 90-91 -0.2855631349707046 1107 92-93 -0.1805790605199853 1107 94-95 -0.2672542740978585 1107 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54-55 -0.1949878955570199 2316 56-57 -0.17417375463009677 2316 58-59 -0.22298298815001516 2316 60-61 -0.05836307224318915 2316 62-63 -0.1607888435463849 2316 64-65 -0.45723367579583396 2316 66-67 -0.49706852546288616 2316 68-69 -0.1293823244198009 2316 70-71 -0.3139634263701794 2316 72-73 -0.3032742926792906 2316 74-75 -0.1432664602658349 2316 76-77 -0.20188309426812978 2316 78-79 -0.39039031963848814 2316 80-81 -0.1555938240829704 2316 82-83 -0.2774097123329433 2316 84-85 -0.3098272913084301 2316 86-87 -0.39328540200756734 2316 88-89 -0.3544487119513775 2316 90-91 -0.028054896868493273 2316 92-93 -0.0245748672838495 2316 94-95 1.5730965218452297E-4 2316 96-97 0.2823335466294914 2316 98-99 0.3489473634955509 2316 100-101 -0.06043260581513721 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 21.0 5 43.0 6 114.0 7 279.0 8 632.0 9 1300.0 10 2166.0 11 3616.0 12 5349.0 13 7455.0 14 9889.0 15 14043.0 16 18771.0 17 26191.0 18 23788.0 19 14608.0 20 11990.0 21 12151.0 22 12923.0 23 14220.0 24 16373.0 25 19619.0 26 23989.0 27 29976.0 28 37308.0 29 46971.0 30 58413.0 31 74229.0 32 94050.0 33 122948.0 34 167868.0 35 239604.0 36 368835.0 37 585379.0 38 512432.0 39 52346.0 40 2.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.87447784066094 20.35379005884707 15.82703952204064 21.94469257845135 2 15.387013832433555 21.26946742583195 39.92271967134947 23.420799070385026 3 19.220534878432918 25.316456177521175 30.35853752947824 25.10447141456767 4 13.006966788927057 17.841816399521505 37.61856887660623 31.532647934945203 5 13.4276426029328 37.8948473321635 34.26923817064813 14.40827189425557 6 30.91118085589371 37.32920492215311 17.530094231504854 14.229519990448328 7 27.698936768082206 33.60150514885323 20.882837637542938 17.81672044552163 8 26.19899722651684 35.262705206954166 19.75081999128486 18.787477575244136 9 26.138120525980778 17.180309866588388 20.742870657734535 35.9386989496963 10-11 24.436690893134582 27.51050798013313 27.27880768362063 20.773993443111653 12-13 25.61431220722706 24.75733046975619 28.10316026512932 21.52519705788743 14-15 22.595788594725438 26.04066457128125 26.801090989563885 24.562455844429422 16-17 21.377284967599604 29.431698319255517 27.376605855874697 21.814410857270182 18-19 21.328994268236205 27.68363203855955 30.177713576074584 20.80966011712966 20-21 22.27292534409975 26.57728070889226 30.416999695046137 20.73279425196186 22-23 21.906968599844365 26.57106771784444 30.284760628402367 21.237203053908825 24-25 21.77475915913929 26.7691876555786 30.391644094519926 21.06440909076218 26-27 21.53324923546674 26.954614719915128 30.312876384691656 21.19925965992648 28-29 21.332937106839317 27.262227028654934 30.29064087924256 21.114194985263186 30-31 21.549762615089666 26.842461289490956 30.323878496037498 21.28389759938188 32-33 21.409662550676718 26.912577863999704 30.25385698196057 21.42390260336301 34-35 21.853879948560902 26.98682006124934 30.11742625021579 21.041873739973976 36-37 21.828213631970115 26.70947174537797 30.09862329680224 21.36369132584967 38-39 21.564458957848682 27.07490305737163 30.16212366525648 21.198514319523206 40-41 21.809201545606573 27.026118043762782 29.930803610471244 21.233876800159397 42-43 21.597832454877196 27.030899383290137 29.77105884594461 21.600209315888062 44-45 21.77847801404309 27.180686305898387 29.578984952313885 21.46185072774464 46-47 22.010786697319222 27.414059666010875 29.064536907402772 21.510616729267127 48-49 22.036300917219094 27.544254433428417 28.85127833466291 21.568166314689574 50-51 22.083089661759775 27.763748970495524 28.654408180368424 21.49875318737628 52-53 22.264312868977065 27.84133729494514 28.324928723688224 21.56942111238957 54-55 22.063241043596236 27.778787530809083 28.481797448273817 21.67617397732086 56-57 22.175013099327504 28.094179031493898 28.212567293279683 21.51824057589891 58-59 22.286785155058773 27.705648034568537 28.37829565011069 21.629271160262 60-61 22.084743713273404 27.71870933790029 28.743974119227072 21.452572829599234 62-63 21.85216886078818 27.757094740268286 28.84009922788115 21.550637171062387 64-65 21.929095931393732 27.644073142552077 28.663862636889426 21.762968289164764 66-67 21.92911077430144 27.753824661413756 28.395026286110607 21.9220382781742 68-69 21.9498339484377 27.764091188050067 28.529175567491766 21.756899296020464 70-71 22.097557859482432 27.61127203785991 28.443735251374 21.84743485128366 72-73 22.068135277361254 27.539863200147686 28.427672453767737 21.964329068723323 74-75 21.90696169368791 28.049766797748667 28.016457622439738 22.026813886123687 76-77 22.007706739328327 27.77182910720003 28.239982721815913 21.980481431655733 78-79 21.996147390819292 27.809492050286206 28.106194670075663 22.08816588881884 80-81 21.98160314475118 28.018586976112452 28.007483917676147 21.992325961460217 82-83 22.013802449565013 27.877742670763915 28.171898845410396 21.936556034260676 84-85 21.96401760619681 27.8704703788534 28.047361285417395 22.118150729532395 86-87 21.906923669515184 27.842363947608774 28.06547078108495 22.18524160179109 88-89 22.003371983637436 27.648592763391544 27.98638050181261 22.361654751158405 90-91 21.96444634923182 27.573521232917997 28.146738430840973 22.31529398700921 92-93 21.912043068157537 27.511998655450938 28.19716351861189 22.378794757779637 94-95 21.783383132324882 27.55252469099656 28.17233771853443 22.49175445814413 96-97 21.679596152866303 27.607013330514473 28.200380545920652 22.513009970698572 98-99 21.628746030505717 27.585701436233045 28.024159088908497 22.76139344435274 100-101 21.519825660669962 27.63257845488517 28.136650486036864 22.710945398408 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 389.0 1 292.0 2 246.5 3 323.0 4 310.5 5 312.5 6 599.0 7 1326.0 8 2298.0 9 2990.5 10 3001.0 11 2852.0 12 3034.5 13 3296.0 14 3622.0 15 3862.0 16 4060.0 17 4311.5 18 4585.0 19 4968.0 20 5432.5 21 6138.0 22 7198.5 23 8496.0 24 10176.5 25 12872.0 26 16440.0 27 20308.5 28 23560.5 29 27510.0 30 32722.5 31 38056.0 32 43699.0 33 49591.5 34 56653.0 35 63375.5 36 70584.0 37 76801.0 38 80451.5 39 83468.5 40 85215.5 41 89409.0 42 93193.0 43 96370.0 44 101200.0 45 103362.0 46 103366.0 47 105558.0 48 109407.5 49 106730.5 50 99164.0 51 91682.5 52 85057.0 53 81651.0 54 78888.0 55 71183.5 56 61508.0 57 53775.0 58 47707.5 59 41841.5 60 34320.5 61 27300.5 62 21577.5 63 16188.5 64 11901.0 65 8928.0 66 6445.0 67 4880.0 68 3697.5 69 2476.0 70 1651.5 71 1174.0 72 865.5 73 672.0 74 522.5 75 383.0 76 253.5 77 149.0 78 91.0 79 58.5 80 37.0 81 22.0 82 9.0 83 5.0 84 4.5 85 2.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.053157793472466325 2 0.0 3 0.0 4 0.0 5 0.01794740952718462 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0036122964090731756 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011597372681761248 24-25 2.0913294999897337E-4 26-27 0.0012738097863573832 28-29 0.0036883447545273482 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01473436693174585 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.9012086363543033E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.0913294999897337E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.9925381363440363E-4 84-85 0.0013878823045386413 86-87 0.0 88-89 0.0 90-91 0.010437635413585123 92-93 0.0010646768363584097 94-95 0.0 96-97 0.0 98-99 2.0913294999897337E-4 100-101 0.01222477153175817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2629906.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.33963635576741 #Duplication Level Percentage of deduplicated Percentage of total 1 76.24107842296898 42.19153555298648 2 13.617317695676714 15.071548188394118 3 4.273843197665419 7.095387852011234 4 1.9114231723580426 4.231098531211257 5 1.0463397459261947 2.895203052207084 6 0.6139311273047943 2.03848351995202 7 0.41317339660851204 1.600540586413462 8 0.30997939912977596 1.3723317780496869 9 0.22447644257122087 1.1180200232094901 >10 1.2103956297833478 12.401786786190076 >50 0.09223386259060944 3.521112373844186 >100 0.04361223112658058 4.3426763930519305 >500 0.0016467572173681421 0.614602285450193 >1k 3.4307442028200946E-4 0.3677133653934552 >5k 6.86148840564019E-5 0.25767274861214245 >10k+ 1.372297681128038E-4 0.8802869630231797 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12644 0.4807776399612762 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10539 0.40073675637076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6786 0.258032036126006 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2799 0.1064296594631139 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2794 0.10623953859947846 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.8024172727086066E-5 0.0 0.0 0.0 0.0 7 3.8024172727086066E-5 0.0 0.0 0.0 0.0 8 3.8024172727086066E-5 0.0 0.0 0.0 0.0 9 3.8024172727086066E-5 0.0 0.0 3.8024172727086066E-5 0.0 10-11 3.8024172727086066E-5 0.0 0.0 3.8024172727086066E-5 0.0 12-13 3.8024172727086066E-5 0.0 0.0 3.8024172727086066E-5 0.0 14-15 3.8024172727086066E-5 0.0 0.0 3.8024172727086066E-5 1.9012086363543033E-5 16-17 3.8024172727086066E-5 0.0 0.0 3.8024172727086066E-5 3.8024172727086066E-5 18-19 3.8024172727086066E-5 0.0 0.0 3.8024172727086066E-5 3.8024172727086066E-5 20-21 3.8024172727086066E-5 0.0 0.0 7.604834545417213E-5 3.8024172727086066E-5 22-23 3.8024172727086066E-5 0.0 0.0 1.330846045448012E-4 3.8024172727086066E-5 24-25 7.604834545417213E-5 3.8024172727086066E-5 0.0 1.9012086363543032E-4 3.8024172727086066E-5 26-27 7.604834545417213E-5 7.604834545417213E-5 0.0 3.992538136344037E-4 3.8024172727086066E-5 28-29 1.330846045448012E-4 7.604834545417213E-5 0.0 0.0011027010090854958 3.8024172727086066E-5 30-31 1.5209669090834426E-4 1.1407251818125818E-4 0.0 0.002395522881806422 3.8024172727086066E-5 32-33 1.5209669090834426E-4 1.1407251818125818E-4 0.0 0.004220683172706553 3.8024172727086066E-5 34-35 1.5209669090834426E-4 1.1407251818125818E-4 0.0 0.006235964327242115 3.8024172727086066E-5 36-37 1.5209669090834426E-4 1.1407251818125818E-4 0.0 0.008745559727229794 3.8024172727086066E-5 38-39 1.5209669090834426E-4 1.1407251818125818E-4 0.0 0.011160094695399761 3.8024172727086066E-5 40-41 1.5209669090834426E-4 1.1407251818125818E-4 0.0 0.014259064772657273 3.8024172727086066E-5 42-43 1.5209669090834426E-4 1.1407251818125818E-4 0.0 0.01891702593172532 3.8024172727086066E-5 44-45 1.5209669090834426E-4 1.1407251818125818E-4 0.0 0.0264648242180519 3.8024172727086066E-5 46-47 1.9012086363543032E-4 1.1407251818125818E-4 0.0 0.044811487558870926 3.8024172727086066E-5 48-49 1.9012086363543032E-4 1.1407251818125818E-4 0.0 0.07962261769051822 3.8024172727086066E-5 50-51 1.9012086363543032E-4 1.1407251818125818E-4 0.0 0.10808371097674213 3.8024172727086066E-5 52-53 1.9012086363543032E-4 1.1407251818125818E-4 0.0 0.13677294929932857 3.8024172727086066E-5 54-55 1.9012086363543032E-4 1.1407251818125818E-4 0.0 0.1772877053400388 3.8024172727086066E-5 56-57 1.9012086363543032E-4 1.1407251818125818E-4 0.0 0.20820135776715976 3.8024172727086066E-5 58-59 1.9012086363543032E-4 1.330846045448012E-4 0.0 0.25221433769876184 3.8024172727086066E-5 60-61 1.9012086363543032E-4 1.5209669090834426E-4 0.0 0.32512568890294935 3.8024172727086066E-5 62-63 1.9012086363543032E-4 1.711087772718873E-4 0.0 0.37777015604360004 3.8024172727086066E-5 64-65 1.9012086363543032E-4 2.2814503636251637E-4 0.0 0.44600453400235596 3.8024172727086066E-5 66-67 1.9012086363543032E-4 2.2814503636251637E-4 0.0 0.5632710826926894 3.8024172727086066E-5 68-69 1.9012086363543032E-4 2.2814503636251637E-4 0.0 0.6919068590284215 3.8024172727086066E-5 70-71 2.0913294999897334E-4 2.2814503636251637E-4 0.0 0.8088502022505748 3.8024172727086066E-5 72-73 2.2814503636251637E-4 2.2814503636251637E-4 0.0 0.9390449696681173 3.8024172727086066E-5 74-75 2.2814503636251637E-4 2.2814503636251637E-4 0.0 1.0602470202357042 3.8024172727086066E-5 76-77 2.2814503636251637E-4 2.2814503636251637E-4 0.0 1.172304257262427 3.8024172727086066E-5 78-79 2.2814503636251637E-4 2.2814503636251637E-4 0.0 1.3119860557753775 3.8024172727086066E-5 80-81 2.2814503636251637E-4 2.2814503636251637E-4 0.0 1.476516651165479 5.70362590906291E-5 82-83 2.2814503636251637E-4 2.2814503636251637E-4 0.0 1.6862960121008128 7.604834545417213E-5 84-85 2.4715712272605937E-4 2.2814503636251637E-4 0.0 1.9233577169678306 7.604834545417213E-5 86-87 2.661692090896024E-4 2.2814503636251637E-4 0.0 2.2879144729887684 7.604834545417213E-5 88-89 2.661692090896024E-4 2.2814503636251637E-4 0.0 2.7608401212819014 7.604834545417213E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9380 0.0 34.65531 1 GTATCAA 14565 0.0 34.19537 1 ATCAACG 17955 0.0 27.541248 3 TCAACGC 18115 0.0 27.29799 4 TATCAAC 18215 0.0 27.252441 2 CAACGCA 18415 0.0 26.827482 5 AACGCAG 18985 0.0 26.044071 6 ACGCAGA 21415 0.0 22.957035 7 CGCAGAG 21435 0.0 22.824816 8 GAGTACT 11955 0.0 22.309698 12-13 GTACATG 11035 0.0 21.878014 1 GTACTTT 12215 0.0 21.639162 14-15 AGTACTT 12110 0.0 21.259287 12-13 TACATGG 11095 0.0 21.193182 2 GCAGAGT 22780 0.0 21.080988 9 AGAGTAC 18205 0.0 20.78206 10-11 GCACCGT 355 3.6379788E-12 20.069275 6 ACATGGG 11000 0.0 19.951134 3 TACTTTT 13735 0.0 18.639269 14-15 ACTTTTT 14335 0.0 18.505571 16-17 >>END_MODULE