FastQCFastQC Report
Thu 26 May 2016
SRR937492_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937492_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024440
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123710.4090343997566492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90680.29982409966803775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59260.1959370990993374No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46150.1525902315800611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA153100.035.602421
GGTATCA95700.035.1570631
ATCAACG191350.028.3296013
TATCAAC192100.028.2199332
TCAACGC192700.028.132534
CAACGCA195700.027.7736455
AACGCAG199550.027.235546
ACGCAGA224400.024.0704867
CGCAGAG223600.023.943758
GTACATG133750.023.4499241
TACATGG133050.023.1039942
GCAGAGT232250.022.3550539
AGAGTAC188050.021.7238310-11
AGTACTT118150.021.71093412-13
ACATGGG135050.021.6353443
GAGTACT116050.021.5512112-13
GTACTTT117750.020.99801814-15
CGCGGAA1503.7380232E-918.99463382-83
GTATAGG10400.018.7344881
TACTTTT135250.018.70254914-15
Produced by FastQC (version 0.11.5)