FastQCFastQC Report
Thu 26 May 2016
SRR937492_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937492_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024440
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141710.468549549668699No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117380.38810490537091163No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74000.24467339408287153No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31630.10458134398434091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106350.035.444271
GTATCAA162050.032.4426571
ATCAACG200300.026.0139083
TATCAAC202100.025.993742
TCAACGC202100.025.8292224
CAACGCA205900.025.3063935
AACGCAG211600.024.6467386
GTACATG126000.022.5978241
TACATGG126350.021.7662322
ACGCAGA241200.021.5447527
CGCAGAG241650.021.3277198
ACATGGG126300.021.0226963
GAGTACT133000.020.89323612-13
GTACTTT135100.020.46163714-15
AGAGTAC201400.020.14187210-11
AGTACTT134550.020.12299512-13
GCAGAGT255150.019.8269319
CGTCGAT757.728756E-418.99908644-45
TACTTTT152050.017.83702714-15
GGACCGA5450.017.4303556
Produced by FastQC (version 0.11.5)