##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937491_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 326535 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.699275728482398 33.0 31.0 34.0 30.0 34.0 2 31.76721637802992 33.0 31.0 34.0 30.0 34.0 3 31.72744116250938 33.0 31.0 34.0 30.0 34.0 4 35.41875143552758 37.0 35.0 37.0 33.0 37.0 5 35.459182017241645 37.0 35.0 37.0 33.0 37.0 6 35.554755232976554 37.0 35.0 37.0 33.0 37.0 7 35.513393663772646 37.0 35.0 37.0 33.0 37.0 8 35.511142756519206 37.0 35.0 37.0 33.0 37.0 9 37.06494862725282 39.0 37.0 39.0 33.0 39.0 10-11 36.96051418684061 39.0 37.0 39.0 33.0 39.0 12-13 36.8426309583965 39.0 37.0 39.0 33.0 39.0 14-15 37.84459093205935 40.0 37.5 41.0 33.0 41.0 16-17 37.83648919717642 40.0 37.5 41.0 33.0 41.0 18-19 37.9772459307578 40.0 38.0 41.0 33.5 41.0 20-21 37.9816283093696 40.0 38.0 41.0 33.0 41.0 22-23 37.968144303060924 40.0 38.0 41.0 33.0 41.0 24-25 37.88965501401075 40.0 38.0 41.0 33.0 41.0 26-27 37.751888771494635 40.0 37.0 41.0 33.0 41.0 28-29 37.58953557811567 40.0 37.0 41.0 33.0 41.0 30-31 37.1548915123953 39.0 36.0 41.0 32.5 41.0 32-33 36.680865757116386 39.0 35.0 41.0 31.5 41.0 34-35 36.444771617131394 39.0 35.0 41.0 31.0 41.0 36-37 36.146711684811734 39.0 35.0 41.0 30.5 41.0 38-39 35.83370848454224 38.5 35.0 41.0 30.0 41.0 40-41 35.43308680539605 38.5 35.0 40.5 28.5 41.0 42-43 34.875507985361445 38.0 35.0 40.0 25.0 41.0 44-45 34.16662226101337 38.0 35.0 40.0 18.0 41.0 46-47 33.150786286309284 38.0 33.5 40.0 2.0 41.0 48-49 32.390739124443016 38.0 33.0 40.0 2.0 41.0 50-51 31.297373941537657 37.5 31.5 39.5 2.0 40.5 52-53 31.295283813373757 37.5 31.5 39.5 2.0 40.5 54-55 31.486496087708822 38.0 32.0 40.0 2.0 41.0 56-57 31.2159064112576 38.0 31.0 40.0 2.0 41.0 58-59 30.936758693555056 37.5 31.0 40.0 2.0 41.0 60-61 30.627856125683312 37.0 30.5 40.0 2.0 41.0 62-63 30.23064602569403 36.5 29.5 40.0 2.0 41.0 64-65 29.812327928093467 36.0 28.0 39.0 2.0 41.0 66-67 29.35911004945871 35.5 28.0 39.0 2.0 40.5 68-69 28.830613869876125 35.0 26.5 38.5 2.0 40.0 70-71 28.375612108962287 35.0 26.0 38.0 2.0 40.0 72-73 27.91686955456536 35.0 25.5 37.0 2.0 39.5 74-75 27.412471863659334 34.0 24.0 37.0 2.0 39.0 76-77 26.914574241658627 34.0 21.5 36.0 2.0 39.0 78-79 26.411375503391675 34.0 19.5 36.0 2.0 38.0 80-81 25.867345001301544 33.5 16.5 35.0 2.0 37.0 82-83 25.348405224554796 33.0 9.0 35.0 2.0 37.0 84-85 24.89338202642902 33.0 4.5 35.0 2.0 36.0 86-87 24.37857197543908 32.0 2.0 35.0 2.0 36.0 88-89 23.836677538395577 32.0 2.0 35.0 2.0 35.0 90-91 23.334621097279005 31.0 2.0 35.0 2.0 35.0 92-93 22.76906610317424 31.0 2.0 34.0 2.0 35.0 94-95 22.088978210605298 31.0 2.0 34.0 2.0 35.0 96-97 21.32405408302326 30.0 2.0 34.0 2.0 35.0 98-99 20.322920973249424 29.0 2.0 34.0 2.0 35.0 100-101 17.734124978945594 23.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06392592123562224 1101 2 0.16890346182597327 1101 3 0.04517099445727979 1101 4 0.1841879818439054 1101 5 0.13416882820855136 1101 6 0.12918056597747807 1101 7 0.12170103911427788 1101 8 0.18993849240998628 1101 9 0.07617521056544518 1101 10-11 0.3001648490956157 1101 12-13 0.35135365021636744 1101 14-15 0.26460895990609146 1101 16-17 0.3903602047049972 1101 18-19 0.41941328076990914 1101 20-21 0.2025580302754193 1101 22-23 0.2751993886703943 1101 24-25 0.22873207103740612 1101 26-27 0.19728402047142168 1101 28-29 0.27854523700150224 1101 30-31 0.24380740333243267 1101 32-33 0.1548431666841239 1101 34-35 0.3347428146669955 1101 36-37 0.6894629994751469 1101 38-39 0.8731225968652794 1101 40-41 0.7406694637634104 1101 42-43 0.7737129222517325 1101 44-45 1.453745652710765 1101 46-47 1.618855953321848 1101 48-49 1.75145206606172 1101 50-51 1.6456125709453424 1101 52-53 1.701253904236495 1101 54-55 1.8278025941614224 1101 56-57 1.4683146867480197 1101 58-59 1.561186054515133 1101 60-61 1.5055137432751486 1101 62-63 1.671063320590921 1101 64-65 1.5273716064514495 1101 66-67 1.784802790352753 1101 68-69 1.9662875686516017 1101 70-71 2.1324752485850134 1101 72-73 2.3869677568042427 1101 74-75 2.4602701792985116 1101 76-77 2.316258222077213 1101 78-79 2.232459314333507 1101 80-81 2.1101245180238912 1101 82-83 1.9988232852029064 1101 84-85 2.5309079386825033 1101 86-87 2.5678709721104056 1101 88-89 2.626168083406796 1101 90-91 2.5854964173656967 1101 92-93 2.776445290083636 1101 94-95 2.665715198302564 1101 96-97 2.9453871070950406 1101 98-99 3.33654417330418 1101 100-101 3.428826192365495 1102 1 -0.02261585828799184 1102 2 0.006535994879467211 1102 3 -0.2522135605672773 1102 4 -0.1369308027517704 1102 5 -0.13670675262065402 1102 6 -0.1053349881914869 1102 7 -0.26425317523592184 1102 8 -0.08210465557796454 1102 9 -0.0607563094935486 1102 10-11 0.038984329977296284 1102 12-13 -0.027788967875721937 1102 14-15 0.17660653968590623 1102 16-17 0.06358084653604124 1102 18-19 0.06742723485189828 1102 20-21 0.1406840613506617 1102 22-23 0.03682188159172739 1102 24-25 -0.07690108597910239 1102 26-27 -0.020526342041634393 1102 28-29 0.05914424149686681 1102 30-31 -0.06638966498678656 1102 32-33 0.06865591160143225 1102 34-35 0.06934345470178727 1102 36-37 0.34644642191229735 1102 38-39 0.6346741848984223 1102 40-41 0.4040721420773892 1102 42-43 0.48105535977271074 1102 44-45 -0.17666970812421567 1102 46-47 0.32399088532405784 1102 48-49 0.4383148083791788 1102 50-51 0.31785708881573527 1102 52-53 0.20095610370612604 1102 54-55 0.329111971096534 1102 56-57 0.5115099446290969 1102 58-59 0.49469381387339695 1102 60-61 0.4684399407405344 1102 62-63 0.5697993227842453 1102 64-65 0.510236258473892 1102 66-67 0.7113264182249921 1102 68-69 0.6789455092900276 1102 70-71 0.6515675714764164 1102 72-73 1.0214766931836508 1102 74-75 0.8841466913452543 1102 76-77 0.7928596089768298 1102 78-79 0.7398097877418124 1102 80-81 0.6759833416645264 1102 82-83 0.9263655799687065 1102 84-85 0.8154681629310296 1102 86-87 0.7338640423393379 1102 88-89 0.36723997623641935 1102 90-91 0.7108917121173164 1102 92-93 0.9139062333077277 1102 94-95 0.4957349098614827 1102 96-97 0.12913476132498758 1102 98-99 0.2779105577693919 1102 100-101 0.6336538698522496 1103 1 0.33324159452480373 1103 2 0.3565414309208954 1103 3 0.3277317008590437 1103 4 0.2791107191507507 1103 5 0.24895911518427027 1103 6 0.3653836564852071 1103 7 0.25856638127763887 1103 8 0.21863606415391956 1103 9 0.46028117083034203 1103 10-11 0.3685975201772962 1103 12-13 0.27960972085655555 1103 14-15 0.30655156476260004 1103 16-17 0.3693889022767394 1103 18-19 0.23950158099065533 1103 20-21 0.21690681614737883 1103 22-23 0.34915082354898885 1103 24-25 0.3093060224722848 1103 26-27 0.29993302709394243 1103 28-29 0.14499115311628685 1103 30-31 0.338730140639278 1103 32-33 0.3833199878761775 1103 34-35 0.5863984438060683 1103 36-37 0.7523769067599204 1103 38-39 1.0243367249006 1103 40-41 1.2903383379355944 1103 42-43 1.2516378670640904 1103 44-45 1.129242341452482 1103 46-47 0.8451252689951332 1103 48-49 0.958957584825523 1103 50-51 1.0838024164199531 1103 52-53 1.075426089666962 1103 54-55 1.2913787531018137 1103 56-57 1.3446943776972482 1103 58-59 1.2079851715129273 1103 60-61 1.389619703540049 1103 62-63 1.6258094574562634 1103 64-65 1.381676242213036 1103 66-67 1.6357961678361974 1103 68-69 2.04134275628957 1103 70-71 2.053005049909512 1103 72-73 1.954296675126539 1103 74-75 1.8443761395634155 1103 76-77 1.5988189284789804 1103 78-79 1.66844165428936 1103 80-81 1.701735997052591 1103 82-83 1.5882272587128377 1103 84-85 1.7604885126339376 1103 86-87 1.743367660852126 1103 88-89 1.9539826529432176 1103 90-91 1.9122072341427412 1103 92-93 2.134061184123368 1103 94-95 2.3047880459846866 1103 96-97 2.1109500210023207 1103 98-99 2.310054107079015 1103 100-101 2.350459746449385 1104 1 -0.0713077864138647 1104 2 0.1658321497998685 1104 3 -0.14056660032149182 1104 4 -0.13859731424976474 1104 5 0.1031917670702498 1104 6 0.034051474893885825 1104 7 0.042091671612602966 1104 8 0.0116296335943602 1104 9 -0.06724546320363345 1104 10-11 0.14258734645621018 1104 12-13 0.24476712559788893 1104 14-15 0.3992859922508458 1104 16-17 0.20220155224314595 1104 18-19 0.17878558137457645 1104 20-21 0.169519830832094 1104 22-23 0.06397950192502577 1104 24-25 0.08772045763880953 1104 26-27 0.37041723863055864 1104 28-29 0.46011016645730507 1104 30-31 0.6021095436530004 1104 32-33 0.34696333176714944 1104 34-35 0.44295857433682784 1104 36-37 0.8311056715166174 1104 38-39 0.7042580225299204 1104 40-41 0.614918431994532 1104 42-43 0.7947226160803211 1104 44-45 0.971784811939095 1104 46-47 1.009397272066444 1104 48-49 0.561522610259825 1104 50-51 0.7205453859947717 1104 52-53 0.619580998967276 1104 54-55 0.900206375714351 1104 56-57 0.6848061926506972 1104 58-59 0.9671079280154693 1104 60-61 0.8714773674233385 1104 62-63 0.862595617356444 1104 64-65 0.8382387722063207 1104 66-67 0.7648968492835522 1104 68-69 0.7753215450408888 1104 70-71 0.7323840690092354 1104 72-73 1.021006532118573 1104 74-75 0.9640205392178913 1104 76-77 0.8924243608621261 1104 78-79 0.9101035220309832 1104 80-81 0.7527941600240844 1104 82-83 0.4930213848285909 1104 84-85 0.6938314438173556 1104 86-87 0.7314783240635698 1104 88-89 0.6115817507453123 1104 90-91 0.8225177245928776 1104 92-93 0.9451347036549933 1104 94-95 1.1886708562351913 1104 96-97 1.0560503185356787 1104 98-99 1.0915638488968469 1104 100-101 1.3116028503940989 1105 1 0.27216057052383746 1105 2 0.1685062114023239 1105 3 0.29090809513260396 1105 4 0.39822550440923976 1105 5 0.4154874984756134 1105 6 0.5312734220068904 1105 7 0.22247693456198903 1105 8 0.2857110439142616 1105 9 0.44279712370337165 1105 10-11 0.3718534651053105 1105 12-13 0.1407832690068176 1105 14-15 0.2181331745822277 1105 16-17 0.26987264266604427 1105 18-19 0.4931660904366666 1105 20-21 0.29913011111336374 1105 22-23 0.42969208982242435 1105 24-25 0.4821049282736567 1105 26-27 0.6955014220513718 1105 28-29 0.582554952037178 1105 30-31 0.8072744441184838 1105 32-33 0.7100810895169758 1105 34-35 0.828914798958003 1105 36-37 0.7343120694565997 1105 38-39 0.7885107708710208 1105 40-41 0.35841650072782016 1105 42-43 0.8310502381462754 1105 44-45 0.8453264579725683 1105 46-47 1.3824140097112902 1105 48-49 1.513946597582791 1105 50-51 1.2721770587688646 1105 52-53 1.025033113456729 1105 54-55 1.1703732569662044 1105 56-57 1.3392208702777104 1105 58-59 1.2868167524187548 1105 60-61 1.3604419113472765 1105 62-63 1.6330001929729683 1105 64-65 1.7779461110198262 1105 66-67 1.6306193411128085 1105 68-69 1.6079475725738206 1105 70-71 1.8991811374454066 1105 72-73 2.0103944691295688 1105 74-75 1.944437989945829 1105 76-77 2.037049001844597 1105 78-79 2.085687854010782 1105 80-81 1.9181972500508238 1105 82-83 1.7831917324565971 1105 84-85 1.7942573128873747 1105 86-87 1.5484660431880606 1105 88-89 1.8241622201916812 1105 90-91 1.8199847940194402 1105 92-93 2.3363672479750903 1105 94-95 2.045853682817345 1105 96-97 2.000489260795753 1105 98-99 2.208712506914079 1105 100-101 2.0150345627839172 1106 1 -0.0814996979393996 1106 2 -0.8943434980370633 1106 3 -0.22064900301041845 1106 4 -0.1866619353058212 1106 5 -0.29085030167094317 1106 6 -0.16170370238032206 1106 7 0.032729208205900306 1106 8 0.1732473284875624 1106 9 0.25131682672372335 1106 10-11 -0.4473400090680215 1106 12-13 -0.385892638036502 1106 14-15 -0.15166272291775584 1106 16-17 -0.08585818970632886 1106 18-19 0.07520756988156307 1106 20-21 -9.426000472316787E-4 1106 22-23 -0.1279428660680324 1106 24-25 -0.09813013327521247 1106 26-27 0.24026707057243613 1106 28-29 0.37685905510524975 1106 30-31 0.41816180430571137 1106 32-33 0.4311116566072215 1106 34-35 0.5581054896572226 1106 36-37 0.0772105180958178 1106 38-39 0.4262898993595883 1106 40-41 0.5557619753101548 1106 42-43 0.0980620934737928 1106 44-45 0.47957511878068715 1106 46-47 0.537007594087477 1106 48-49 0.8865117678007586 1106 50-51 0.9441900786110544 1106 52-53 0.9170059149382155 1106 54-55 1.008102396531875 1106 56-57 1.1204426449980893 1106 58-59 0.90117493441252 1106 60-61 1.083764584164097 1106 62-63 1.3588251522149513 1106 64-65 1.4896293175820112 1106 66-67 1.602455127501056 1106 68-69 1.7418156359006964 1106 70-71 1.4875450795264236 1106 72-73 1.5147883818420915 1106 74-75 1.3990986049231928 1106 76-77 1.5393098644943528 1106 78-79 1.618725987606176 1106 80-81 1.2251377575520017 1106 82-83 1.6282431131165644 1106 84-85 1.1575092059338026 1106 86-87 0.9123712005679607 1106 88-89 0.9551331982135984 1106 90-91 0.8426928303068522 1106 92-93 1.2823053639607522 1106 94-95 1.0853449319155537 1106 96-97 1.1521687181887152 1106 98-99 0.7946593709612522 1106 100-101 0.5433549917797649 1107 1 0.12046604498029723 1107 2 0.0321313945436934 1107 3 0.30803515513880697 1107 4 0.08861232036954192 1107 5 0.17968697017292357 1107 6 0.3577195704235834 1107 7 0.12128130214944122 1107 8 0.06874333026715362 1107 9 0.35494273847017865 1107 10-11 0.1737773852396387 1107 12-13 0.3452519184127425 1107 14-15 0.39164157502283814 1107 16-17 0.4079425197875395 1107 18-19 0.408546757124725 1107 20-21 0.4352322211158324 1107 22-23 0.39939489282112817 1107 24-25 0.3093371140993071 1107 26-27 0.34405981874895275 1107 28-29 0.25522651673286845 1107 30-31 0.22934979676588085 1107 32-33 0.31844730808884236 1107 34-35 0.7612888458208005 1107 36-37 0.3999921789671177 1107 38-39 0.5050132787046593 1107 40-41 0.4047632982937728 1107 42-43 -0.022209410863958112 1107 44-45 -0.07209148934688159 1107 46-47 0.12119559825546133 1107 48-49 -0.03194034136295443 1107 50-51 -0.16396675934101523 1107 52-53 0.17019336883867808 1107 54-55 0.4263086414828763 1107 56-57 0.672431218166107 1107 58-59 0.4711291568376801 1107 60-61 0.6264834911220554 1107 62-63 0.5366464440557728 1107 64-65 0.26027898449454057 1107 66-67 0.48558474477242797 1107 68-69 0.7214176871106979 1107 70-71 0.5921105438000112 1107 72-73 0.8793347623696448 1107 74-75 1.072751912967636 1107 76-77 0.9519109907866046 1107 78-79 1.009843264835638 1107 80-81 1.0529625617387204 1107 82-83 0.6125253652327523 1107 84-85 0.7126618173629744 1107 86-87 0.4273601366783879 1107 88-89 -0.0322282104712599 1107 90-91 -0.3450215691405347 1107 92-93 0.003600717127131503 1107 94-95 -0.06973392024980996 1107 96-97 0.24185354368366418 1107 98-99 0.5497083681865007 1107 100-101 0.43164775751800377 1108 1 -0.02031757471343454 1108 2 -0.07443645935105536 1108 3 -0.26590236176014415 1108 4 -0.008344639540005971 1108 5 0.0401938157841073 1108 6 0.03565630075320314 1108 7 0.21296578952746614 1108 8 -0.1531552514500234 1108 9 0.044432205815262193 1108 10-11 -0.03847343205505638 1108 12-13 -0.1564564118480476 1108 14-15 0.005614241738662429 1108 16-17 0.1554168948336354 1108 18-19 -0.19244137059447297 1108 20-21 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2316 68-69 -1.585242535663859 2316 70-71 -2.0345809778790844 2316 72-73 -2.2398772416808015 2316 74-75 -1.9179274573720484 2316 76-77 -1.455849296426873 2316 78-79 -1.373890510364582 2316 80-81 -1.738531500674977 2316 82-83 -1.1578879644183537 2316 84-85 -1.0960236286464173 2316 86-87 -1.608426478437142 2316 88-89 -0.823492133881004 2316 90-91 -0.7515160918322437 2316 92-93 -0.7890440403063579 2316 94-95 -1.5246496893743604 2316 96-97 -2.253980940344686 2316 98-99 -1.9918547359283885 2316 100-101 -1.0045807244851268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1685.0 3 260.0 4 245.0 5 228.0 6 277.0 7 359.0 8 455.0 9 592.0 10 822.0 11 1330.0 12 2276.0 13 3547.0 14 4183.0 15 5858.0 16 7917.0 17 11389.0 18 8262.0 19 4069.0 20 3527.0 21 3238.0 22 3401.0 23 3650.0 24 3967.0 25 4236.0 26 4456.0 27 4828.0 28 5650.0 29 6591.0 30 8006.0 31 9540.0 32 11661.0 33 15270.0 34 20327.0 35 28454.0 36 38179.0 37 55380.0 38 38765.0 39 3655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.177257196234116 21.80256139229616 15.541038814188443 18.479142597281285 2 15.154530572882594 22.928817348947632 41.366220412224855 20.55043166594492 3 18.057884488996173 27.87134459820524 34.400240775888015 19.670530136910575 4 10.510733699824943 20.217438039372254 40.24139307760819 29.03043518319462 5 9.434478013962217 41.77423961817361 34.90677010869398 13.88451225917019 6 23.841848498524776 41.697752315688966 19.51288404208613 14.94751514370013 7 23.137123663672146 40.7011230987455 19.922568205682424 16.239185031899932 8 25.926733269064272 33.715161153650136 20.623614582118055 19.73449099516754 9 27.542880303616503 16.04211573536727 22.61955206740483 33.7954518936114 10-11 23.023136909889036 29.08534815913477 28.28033907725661 19.61117585371958 12-13 24.753940558855454 29.76494050205184 28.41485675510119 17.066262183991512 14-15 21.55064296712018 29.058398945094893 30.824351851993985 18.566606235790942 16-17 16.503727717734172 32.94528843187187 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42-43 16.082940396375157 30.102961762322256 36.52164837053955 17.292449470763035 44-45 17.349515203887577 30.640004543284764 35.17684275476546 16.8336374980622 46-47 17.466148227857232 30.901398894454314 34.24812301306484 17.384329864623613 48-49 17.10450973499147 30.851395351336247 34.41411209171447 17.629982821957803 50-51 17.20628876742615 31.05416692255727 33.15528465270528 18.584259657311307 52-53 17.89086553595971 31.969165329422694 32.19151893000008 17.948450204617522 54-55 17.99976358721099 30.650764580451735 32.20663705877928 19.142834773557997 56-57 18.350261363880524 32.36077730815155 31.44776866150385 17.841192666464085 58-59 18.828483344612376 31.556155058459208 31.111898853834337 18.50346274309408 60-61 18.070614016914586 30.493058145227252 32.95949983656294 18.47682800129522 62-63 18.19165426111512 30.08026519744011 32.88678463450943 18.841295906935343 64-65 17.68950298625647 30.27014418738836 33.02621644681517 19.014136379540002 66-67 17.980700763621186 31.174650723107522 31.67616077786235 19.16848773540894 68-69 18.100355122574072 29.780419455928893 32.210875897398125 19.90834952409892 70-71 17.749799344089208 30.752609294166938 32.85048026973065 18.647111092013205 72-73 17.896415345397287 29.9702417036663 33.42168227225081 18.7116606786856 74-75 17.41606144136733 31.288715489879486 32.610337227648614 18.68488584110457 76-77 17.477333112305278 30.76587756580573 32.272245169232285 19.48454415265671 78-79 17.39697031560108 31.099469367769426 31.474611158779837 20.028949157849656 80-81 17.369116556903275 29.697660819610995 32.44663135615982 20.486591267325913 82-83 16.850726981973548 30.44975583104921 32.31470937602632 20.384807810950925 84-85 17.28257333488832 30.01098718979603 31.960753021477196 20.74568645383846 86-87 17.17697644334572 30.24308576672298 31.47501085683858 21.10492693309272 88-89 17.55957862168036 29.927284000638455 31.545821208884306 20.967316168796888 90-91 17.479216256823683 28.55410832869085 32.90975473878552 21.056920675699942 92-93 17.82271976622117 28.109939897721762 33.18026386066585 20.88707647539122 94-95 17.558498401624313 28.347342460623786 33.08277023138464 21.01138890636726 96-97 18.654169058443255 28.27156666226682 31.873351976400453 21.20091230288947 98-99 18.782414634108903 28.11184324632129 31.408293541707916 21.697448577861888 100-101 19.390515827160378 26.835504033712226 32.803894216529855 20.970085922597537 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1225.0 1 768.5 2 355.0 3 413.5 4 363.5 5 386.5 6 617.5 7 1096.0 8 1629.5 9 1724.0 10 1381.5 11 1118.5 12 1056.0 13 1019.5 14 1004.5 15 1018.5 16 1114.0 17 1263.5 18 1492.0 19 1763.5 20 2027.5 21 2254.0 22 2514.0 23 2848.5 24 3201.5 25 3963.5 26 5887.5 27 8659.5 28 9388.0 29 9550.0 30 10222.5 31 10825.5 32 11317.5 33 11285.0 34 12799.5 35 14766.5 36 15990.5 37 16393.5 38 13825.5 39 10533.5 40 9543.0 41 9000.5 42 8266.0 43 7974.0 44 7986.5 45 7700.0 46 7255.0 47 7344.0 48 8148.5 49 7901.0 50 6799.5 51 5949.5 52 5199.5 53 4504.0 54 3788.5 55 3351.5 56 2888.5 57 2390.5 58 2103.0 59 1877.5 60 1668.5 61 1524.5 62 1314.5 63 944.5 64 610.0 65 461.5 66 344.5 67 251.0 68 212.0 69 180.5 70 149.0 71 119.0 72 91.0 73 70.5 74 45.0 75 31.0 76 23.0 77 18.0 78 12.5 79 9.0 80 11.0 81 9.0 82 5.0 83 2.5 84 1.5 85 1.5 86 1.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33288927680034297 2 0.28664614819238365 3 0.28205245992006983 4 0.2832774434593535 5 0.2884836235013092 6 0.2517341173227985 7 0.25387783851654494 8 0.2514278714379775 9 0.2636777068308145 10-11 0.271486976893748 12-13 0.2607683709250157 14-15 0.25066225672592524 16-17 0.2448435849143277 18-19 0.23443122483041637 20-21 0.2393311589875511 22-23 0.23550308542728957 24-25 0.23979052781478247 26-27 0.23596245425452095 28-29 0.2511216255531566 30-31 0.26459644448527725 32-33 0.2572465432495751 34-35 0.2532653467469031 36-37 0.24224049489334987 38-39 0.22983753655810252 40-41 0.2266219547674828 42-43 0.23979052781478247 44-45 0.23871866721790927 46-47 0.24913102730182063 48-49 0.25372471557413445 50-51 0.2679651492183074 52-53 0.2840430581714058 54-55 0.2552559449982391 56-57 0.22784693830676653 58-59 0.21728145528044468 60-61 0.2209564058982957 62-63 0.2266219547674828 64-65 0.2171283323380342 66-67 0.22019079118624343 68-69 0.22417198768891541 70-71 0.22310012709204222 72-73 0.22769381536435607 74-75 0.23305311834872217 76-77 0.24024989664201388 78-79 0.24239361783576033 80-81 0.2322875036366699 82-83 0.22539697122819913 84-85 0.21467836525946682 86-87 0.21544397997151915 88-89 0.22876567596122926 90-91 0.22646883182507235 92-93 0.2318281348094385 94-95 0.22524384828578867 96-97 0.23458434777282683 98-99 0.23749368367862556 100-101 0.22003766824383297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 326535.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.61573144812833 #Duplication Level Percentage of deduplicated Percentage of total 1 78.30365447449064 34.935748194409065 2 11.884827124088014 10.605005105514845 3 4.094741891418442 5.48069713730779 4 1.8406296299805103 3.2848414906671306 5 0.9249072793017797 2.0632707393873617 6 0.5781617181534857 1.5477064770434632 7 0.3719439862101958 1.1616187101750313 8 0.257854363323316 0.9203488821408942 9 0.1871713992314489 0.7515709994592664 >10 1.2181542707551831 10.733616404604284 >50 0.17480372226568675 5.327478826772793 >100 0.14464151136494077 12.963460448452357 >500 0.012339086277577887 4.042020791930033 >1k 0.005484038345590172 4.4460393653364765 >5k 6.855047931987715E-4 1.7365764267992374 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5678 1.7388641340131992 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4135 1.2663267337345154 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3157 0.9668182583796531 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1617 0.49519959575543204 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1234 0.37790742186901866 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1203 0.36841379943957003 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1095 0.3353392438789104 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1088 0.33319552268516395 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 1008 0.3086958518994901 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 989 0.30287718008789255 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 885 0.2710276080665166 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 880 0.269496378642412 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 871 0.2667401656790237 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 861 0.2636777068308145 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 819 0.2508153796683357 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 792 0.2425467407781708 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 780 0.23887179016031973 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 727 0.22264075826481083 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 725 0.22202826649516896 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 723 0.22141577472552712 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 669 0.20487849694519727 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 668 0.2045722510603764 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 606 0.18558500620147916 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 606 0.18558500620147916 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 580 0.17762261319613518 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 526 0.16108533541580536 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 509 0.15587915537384966 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 471 0.1442418117506546 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 471 0.1442418117506546 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 460 0.14087310701762445 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 455 0.13934187759351985 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 451 0.13811689405423616 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 450 0.13781064816941524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 446 0.13658566463013153 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 434 0.13291071401228047 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 428 0.13107323870335494 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 422 0.12923576339442938 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 420 0.12862327162478754 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 420 0.12862327162478754 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 418 0.1280107798551457 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 414 0.126785796315862 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 412 0.12617330454622017 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 408 0.12494832100693647 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 403 0.12341709158283184 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 400 0.12249835392836908 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 383 0.11729217388641341 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 382 0.11698592800159248 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 368 0.11269848561409956 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 364 0.11147350207481588 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 364 0.11147350207481588 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 351 0.10749230557214387 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 345 0.10565483026321834 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 327 0.10014240433644173 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.5312294241046136E-4 0.0 16-17 0.0 0.0 0.0 3.062458848209227E-4 0.0 18-19 0.0 0.0 0.0 3.062458848209227E-4 0.0 20-21 0.0 0.0 0.0 3.062458848209227E-4 0.0 22-23 0.0 0.0 0.0 6.124917696418454E-4 0.0 24-25 0.0 0.0 0.0 0.0019905982513359977 0.0 26-27 0.0 0.0 0.0 0.004746811214724303 0.0 28-29 0.0 0.0 0.0 0.014240433644172906 0.0 30-31 0.0 0.0 0.0 0.02802149846111443 0.0 32-33 0.0 0.0 0.0 0.04302754681733964 0.0 34-35 0.0 0.0 0.0 0.05925857871284855 0.0 36-37 0.0 0.0 0.0 0.07931768416861898 0.0 38-39 0.0 0.0 0.0 0.10932978088106941 0.0 40-41 0.0 0.0 0.0 0.17348829375105274 0.0 42-43 0.0 0.0 0.0 0.24147488018129756 0.0 44-45 0.0 0.0 0.0 0.30410216362717624 0.0 46-47 0.0 0.0 0.0 0.4273661322675977 0.0 48-49 0.0 0.0 0.0 0.5121962423629932 0.0 50-51 0.0 0.0 0.0 0.6294884162494067 0.0 52-53 0.0 0.0 0.0 0.762705376146508 0.0 54-55 0.0 0.0 0.0 0.921340744483746 0.0 56-57 0.0 0.0 0.0 1.272757897315755 0.0 58-59 0.0 0.0 0.0 1.6119252147549266 0.0 60-61 0.0 0.0 0.0 1.9158742554396926 0.0 62-63 0.0 0.0 0.0 2.2440167210253112 0.0 64-65 0.0 0.0 0.0 2.5491907452493607 0.0 66-67 0.0 0.0 0.0 3.001209671245043 0.0 68-69 0.0 0.0 0.0 3.5872111718498783 0.0 70-71 0.0 0.0 0.0 4.0012556081277655 0.0 72-73 0.0 0.0 0.0 4.385441070635613 0.0 74-75 0.0 0.0 0.0 4.958886489962792 0.0 76-77 0.0 0.0 0.0 5.65881145972101 0.0 78-79 0.0 0.0 0.0 6.347099085856033 0.0 80-81 0.0 0.0 0.0 7.040899137917835 0.0 82-83 0.0 0.0 0.0 7.800695178158543 0.0 84-85 0.0 0.0 0.0 8.608418699373726 0.0 86-87 0.0 0.0 0.0 9.464988439217848 0.0 88-89 0.0 0.0 0.0 10.613563630238719 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 195 0.0 46.29029 9 GGTATCA 2625 0.0 43.134033 1 TTAGGCA 230 0.0 41.318058 4 GTATCAA 4550 0.0 40.150616 1 CATAGGG 60 8.246494E-5 39.608654 2 GCATTCC 230 0.0 39.24612 8 TAGGCAT 255 0.0 37.267265 5 TAGGGTC 65 1.323504E-4 36.550587 4 GGCATTC 250 0.0 36.106426 7 AGGCATT 290 0.0 34.402676 6 CCATAGG 70 2.0313643E-4 33.981644 1 TATCAAC 5695 0.0 31.881664 2 GTACTGC 60 0.0039433837 31.672306 6 ATCAACG 5790 0.0 31.435816 3 TCAACGC 5880 0.0 31.030703 4 CAACGCA 5920 0.0 30.821037 5 AGGAGCC 125 2.142533E-7 30.405413 7 AACGCAG 5995 0.0 30.351524 6 CTTTAGG 300 0.0 30.10258 2 CTAGAGG 160 1.884473E-9 29.701921 3 >>END_MODULE