##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937491_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 326535 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03094920912 31.0 31.0 34.0 30.0 34.0 2 32.212338646699436 33.0 31.0 34.0 30.0 34.0 3 32.19994793819958 34.0 31.0 34.0 30.0 34.0 4 35.939225504157285 37.0 35.0 37.0 35.0 37.0 5 35.89039766028144 37.0 35.0 37.0 35.0 37.0 6 35.95857105670142 37.0 35.0 37.0 35.0 37.0 7 35.8964552038832 37.0 35.0 37.0 35.0 37.0 8 35.88407062030104 37.0 35.0 37.0 35.0 37.0 9 37.48974535654677 39.0 37.0 39.0 35.0 39.0 10-11 37.437783086039786 39.0 37.0 39.0 34.0 39.0 12-13 37.32489013428882 39.0 37.0 39.0 34.0 39.0 14-15 38.37278239698654 40.0 38.0 41.0 33.5 41.0 16-17 38.373067205659424 40.0 38.0 41.0 33.5 41.0 18-19 38.570944921677615 40.0 38.0 41.0 34.0 41.0 20-21 38.58352397139663 40.0 38.0 41.0 34.0 41.0 22-23 38.49283997121289 40.0 38.0 41.0 34.0 41.0 24-25 38.42827568254552 40.0 38.0 41.0 34.0 41.0 26-27 38.19869692376009 40.0 37.0 41.0 34.0 41.0 28-29 37.99004394628447 40.0 37.0 41.0 33.0 41.0 30-31 37.68868574578529 39.0 36.5 41.0 33.0 41.0 32-33 37.238309828961675 39.0 35.0 41.0 33.0 41.0 34-35 36.93947356332399 39.0 35.0 41.0 33.0 41.0 36-37 36.58900730396435 39.0 35.0 41.0 31.5 41.0 38-39 36.28031298329429 39.0 35.0 41.0 31.0 41.0 40-41 35.88162677814017 39.0 35.0 41.0 30.5 41.0 42-43 35.31728758019814 39.0 35.0 41.0 28.5 41.0 44-45 34.48090403785199 38.0 35.0 40.5 22.0 41.0 46-47 33.39715497573002 38.0 33.5 40.0 2.0 41.0 48-49 32.52610899291041 38.0 33.0 40.0 2.0 41.0 50-51 32.04164331541795 38.0 33.0 40.0 2.0 41.0 52-53 31.732602936898036 38.0 32.5 40.0 2.0 41.0 54-55 31.442863705268962 38.0 32.0 40.0 2.0 41.0 56-57 31.1068798138025 38.0 31.0 40.0 2.0 41.0 58-59 30.816845054894575 37.5 31.0 40.0 2.0 41.0 60-61 30.47728114903456 37.0 30.0 40.0 2.0 41.0 62-63 30.079256434991656 36.0 29.0 39.5 2.0 41.0 64-65 29.67385732004226 36.0 28.0 39.0 2.0 40.5 66-67 29.204266005175555 35.5 28.0 39.0 2.0 40.0 68-69 28.68139250003828 35.0 26.0 38.0 2.0 40.0 70-71 28.248706876751342 35.0 26.0 37.5 2.0 40.0 72-73 27.78314575772888 34.5 25.5 37.0 2.0 39.0 74-75 27.324949852236358 34.0 24.5 36.5 2.0 39.0 76-77 26.063890547720767 32.5 21.5 35.0 2.0 37.5 78-79 26.559649042215995 34.0 22.0 36.0 2.0 38.0 80-81 26.354652640605142 34.0 20.5 35.0 2.0 37.0 82-83 25.96068874699496 34.0 19.0 35.0 2.0 37.0 84-85 25.564069701563383 33.0 16.0 35.0 2.0 36.0 86-87 25.228871330791492 33.0 8.5 35.0 2.0 36.0 88-89 24.780669759750104 33.0 2.0 35.0 2.0 35.5 90-91 24.29951460027256 32.0 2.0 35.0 2.0 35.0 92-93 23.86054328019967 32.0 2.0 35.0 2.0 35.0 94-95 23.406239759903226 32.0 2.0 34.0 2.0 35.0 96-97 22.811782810418485 31.0 2.0 34.0 2.0 35.0 98-99 21.993342214463993 31.0 2.0 34.0 2.0 35.0 100-101 19.697386191373052 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11566786527440343 1101 2 0.06858787569515812 1101 3 0.10279544932795659 1101 4 0.0518776755696706 1101 5 -0.05540054435498831 1101 6 -0.011758825425260966 1101 7 -0.015722881095392438 1101 8 0.06244939280524875 1101 9 0.14751460785631565 1101 10-11 -0.0012534306159963648 1101 12-13 0.08945940855539192 1101 14-15 0.2697251555810496 1101 16-17 0.05790379001483359 1101 18-19 0.14568179345202026 1101 20-21 0.1071188965421328 1101 22-23 0.13614860737797585 1101 24-25 0.2435249595764617 1101 26-27 0.23501299494800065 1101 28-29 0.26195494443410183 1101 30-31 0.7103372615190295 1101 32-33 0.539783552305046 1101 34-35 0.6583071302093018 1101 36-37 0.7282470104851555 1101 38-39 1.0541307084464577 1101 40-41 1.239839074584033 1101 42-43 1.6851030491529855 1101 44-45 1.691332784120931 1101 46-47 1.6787359124577321 1101 48-49 1.746507721338027 1101 50-51 1.758286417335853 1101 52-53 1.958630526833435 1101 54-55 2.0919777584576345 1101 56-57 1.7926093087874513 1101 58-59 2.116099397963584 1101 60-61 1.866059356408016 1101 62-63 1.7793068692209353 1101 64-65 2.071646142234499 1101 66-67 1.8614782464896429 1101 68-69 1.9456443812255984 1101 70-71 2.0321970796175677 1101 72-73 2.0633454299359677 1101 74-75 2.19945326372806 1101 76-77 1.988851654016237 1101 78-79 2.0216464887850414 1101 80-81 1.866133411025345 1101 82-83 1.580297422238523 1101 84-85 1.6060706446285167 1101 86-87 1.767037704340332 1101 88-89 1.6704381080147037 1101 90-91 1.6011117578389538 1101 92-93 1.5559376745494617 1101 94-95 1.71895885557959 1101 96-97 1.6848836564333247 1101 98-99 1.9511937948201954 1101 100-101 2.7266572368852984 1102 1 0.20986737272178146 1102 2 -0.07613372807887231 1102 3 0.204061810630904 1102 4 0.15753068595487463 1102 5 0.005303493914745161 1102 6 0.11175276279696789 1102 7 0.2488941616035305 1102 8 0.2934244295576178 1102 9 0.19154654341795663 1102 10-11 0.22204969723497214 1102 12-13 0.1784332257436816 1102 14-15 0.27797375757300813 1102 16-17 0.3842340838919611 1102 18-19 0.3505921933083158 1102 20-21 0.15500569452465385 1102 22-23 -0.03527577728577569 1102 24-25 -0.17926198068853694 1102 26-27 0.04956598689793168 1102 28-29 0.14590964093754621 1102 30-31 0.2772454775047777 1102 32-33 0.4523578251754188 1102 34-35 0.36143545387593434 1102 36-37 0.5926083228873651 1102 38-39 0.28961529608725556 1102 40-41 0.13547653316376085 1102 42-43 0.4468035374513164 1102 44-45 0.6922120047344009 1102 46-47 0.7681564304415716 1102 48-49 0.9744787543277056 1102 50-51 0.9233199080780423 1102 52-53 0.8934358456454454 1102 54-55 1.1420366095148289 1102 56-57 1.451407896362074 1102 58-59 1.1610767320343314 1102 60-61 1.460549573896941 1102 62-63 1.3437735935569144 1102 64-65 1.3440839941000071 1102 66-67 1.4644817775530719 1102 68-69 1.28779355565635 1102 70-71 1.3844265656516725 1102 72-73 1.426945696538347 1102 74-75 1.073322921635608 1102 76-77 1.0431128014464086 1102 78-79 1.0841302397481662 1102 80-81 1.4311366490152366 1102 82-83 1.296172081304185 1102 84-85 1.7443635385406253 1102 86-87 1.7033580242631885 1102 88-89 1.8405932951799713 1102 90-91 1.7520493568103603 1102 92-93 1.6410920817609593 1102 94-95 1.8694615712368048 1102 96-97 1.6887030664101133 1102 98-99 1.4624747625301104 1102 100-101 1.5724272876059295 1103 1 0.1178753707931719 1103 2 0.20324571233975064 1103 3 0.29705593497916993 1103 4 0.2086105674019052 1103 5 0.11016236955229886 1103 6 0.2884619251266187 1103 7 0.10127491139908784 1103 8 0.07569442591783115 1103 9 0.039346837436895044 1103 10-11 0.16541323605067504 1103 12-13 0.18438214586223722 1103 14-15 0.3116677604375582 1103 16-17 0.19697663769695595 1103 18-19 0.3046221908030091 1103 20-21 0.015507417513433097 1103 22-23 0.2685989385037999 1103 24-25 0.18392231056984087 1103 26-27 0.057308800687515316 1103 28-29 0.169239712646025 1103 30-31 0.1341783211216807 1103 32-33 0.5486135743801057 1103 34-35 0.6406470860591966 1103 36-37 0.6807856418317257 1103 38-39 0.753909957894578 1103 40-41 1.1360863152021423 1103 42-43 1.5129176186894142 1103 44-45 1.515836095379207 1103 46-47 1.9568816078219626 1103 48-49 1.934145690432949 1103 50-51 1.906189287093028 1103 52-53 1.9111691581800123 1103 54-55 2.0643839394730925 1103 56-57 1.9681059975291681 1103 58-59 1.741927212533124 1103 60-61 2.0890174138031625 1103 62-63 2.2793068692209353 1103 64-65 2.265906627885716 1103 66-67 2.2433767012357784 1103 68-69 1.8275428359717374 1103 70-71 1.6911374769685636 1103 72-73 1.6052880347924798 1103 74-75 1.5736254491585235 1103 76-77 1.4866441484974757 1103 78-79 1.8031034424274281 1103 80-81 2.0603938966765583 1103 82-83 1.8540281065652309 1103 84-85 1.9636865386682523 1103 86-87 2.260415187784041 1103 88-89 2.177060624570995 1103 90-91 2.3980212501126843 1103 92-93 2.3517434140638116 1103 94-95 2.6483186789791517 1103 96-97 2.563470852901318 1103 98-99 2.558257812480239 1103 100-101 2.931955250130329 1104 1 0.11131044133736623 1104 2 0.273386800735949 1104 3 0.0596339175110856 1104 4 -0.010796285463058553 1104 5 0.13845683159279787 1104 6 0.10637151337885342 1104 7 0.1555555525354677 1104 8 0.21338998211324878 1104 9 0.2666047316685862 1104 10-11 0.13057543971704888 1104 12-13 0.21994937880206322 1104 14-15 0.40543395680957417 1104 16-17 0.2621388413633312 1104 18-19 0.24100044207472848 1104 20-21 0.3189266516049969 1104 22-23 0.3415234034236576 1104 24-25 0.24739289315934343 1104 26-27 0.13055239412258857 1104 28-29 0.06446238276792116 1104 30-31 0.5241917581117974 1104 32-33 0.35844897125120667 1104 34-35 0.43750379015747143 1104 36-37 0.09254780710670474 1104 38-39 0.13236038499891123 1104 40-41 0.21403765410222775 1104 42-43 0.3179444926696391 1104 44-45 0.5287356058888619 1104 46-47 0.45238741452126163 1104 48-49 0.8040756261273572 1104 50-51 0.7333632001365089 1104 52-53 0.4494501832304003 1104 54-55 0.6554315448094954 1104 56-57 0.9047385918280462 1104 58-59 0.9791476751494947 1104 60-61 1.2210886298507617 1104 62-63 1.3674655216827851 1104 64-65 1.1528727474749267 1104 66-67 1.065394169322925 1104 68-69 0.9258440165072983 1104 70-71 1.1789266122022717 1104 72-73 1.379433826135216 1104 74-75 1.1865153618180848 1104 76-77 1.034873345157422 1104 78-79 0.9223855232413243 1104 80-81 0.75184701117891 1104 82-83 0.5893337980903297 1104 84-85 0.7773562766469446 1104 86-87 0.7863437797794326 1104 88-89 0.8410494910648971 1104 90-91 0.8625116042733545 1104 92-93 0.8399236616883421 1104 94-95 0.9768770818968946 1104 96-97 1.3063876395411071 1104 98-99 1.4302896773425111 1104 100-101 2.2086948604576158 1105 1 -0.034653706429295994 1105 2 -0.03486317450422405 1105 3 -0.23044234276424547 1105 4 -0.012678032516639348 1105 5 -0.08580780146652245 1105 6 -0.03836454683705881 1105 7 -0.017035316039354598 1105 8 -0.06480651189487219 1105 9 0.0437266490697894 1105 10-11 0.0584673878204498 1105 12-13 -0.1828658138294159 1105 14-15 -0.24360536179990788 1105 16-17 -0.026142331262200003 1105 18-19 0.11562904462994084 1105 20-21 0.011758680920635811 1105 22-23 -0.1347962954968125 1105 24-25 -0.17802059599585363 1105 26-27 -0.07340670263882743 1105 28-29 -0.022343104010332127 1105 30-31 -0.0032074693815218325 1105 32-33 0.0496418998438557 1105 34-35 -0.05208222549074293 1105 36-37 -0.270971059883486 1105 38-39 -0.20092920596870556 1105 40-41 -0.17989371164998147 1105 42-43 -0.35069223110970427 1105 44-45 0.2086207202792707 1105 46-47 0.5494434707755929 1105 48-49 0.4128802215517382 1105 50-51 0.4175788087331185 1105 52-53 0.5575961671860306 1105 54-55 0.7779060421849522 1105 56-57 0.8526117224609102 1105 58-59 0.714039227095622 1105 60-61 0.715428532792231 1105 62-63 1.0478582125810725 1105 64-65 1.2089389057397568 1105 66-67 1.2063469275679495 1105 68-69 1.185317127332297 1105 70-71 0.7349506787566931 1105 72-73 0.9305405905206818 1105 74-75 1.1730361105553335 1105 76-77 1.0359132580028216 1105 78-79 1.167022544138618 1105 80-81 1.2593987592228828 1105 82-83 1.261815723414685 1105 84-85 1.2106656754782819 1105 86-87 1.3666696191376566 1105 88-89 1.4598174419413965 1105 90-91 1.9246018290926052 1105 92-93 1.408404398540874 1105 94-95 1.9415448049829607 1105 96-97 2.0566748535650774 1105 98-99 1.6931796446595335 1105 100-101 2.1390157478499106 1106 1 -0.057745180477660085 1106 2 -0.02538800138917452 1106 3 0.12717159505635323 1106 4 0.190873453233678 1106 5 0.15476389003365654 1106 6 0.09416290446635145 1106 7 0.07192224450120932 1106 8 -0.06027910603594222 1106 9 -0.12346357582355694 1106 10-11 -0.031836465303008765 1106 12-13 -0.285610092085939 1106 14-15 -0.2216017148807623 1106 16-17 -0.1536396471978705 1106 18-19 -0.07306493795346825 1106 20-21 0.0800645676843672 1106 22-23 0.11653153526795279 1106 24-25 0.06788514080110275 1106 26-27 0.2715992335457713 1106 28-29 0.2201031720989377 1106 30-31 0.3961585801870413 1106 32-33 0.477447670156117 1106 34-35 -0.20823512342813189 1106 36-37 0.1373759541304551 1106 38-39 0.430064494290896 1106 40-41 0.5316702031643459 1106 42-43 0.3884990844510554 1106 44-45 0.8971840499959782 1106 46-47 1.0511107149658088 1106 48-49 1.4939162419790293 1106 50-51 1.8563139753723306 1106 52-53 1.8109974021396518 1106 54-55 1.6169814743335174 1106 56-57 1.8267623368134132 1106 58-59 1.9048009333084472 1106 60-61 1.7868644077972036 1106 62-63 2.0697397274726583 1106 64-65 1.949294735981919 1106 66-67 2.0703600155768562 1106 68-69 2.5848053089284164 1106 70-71 2.2877117146635086 1106 72-73 1.9982102546291998 1106 74-75 2.049640158521978 1106 76-77 2.413383591281246 1106 78-79 2.501633056581884 1106 80-81 2.278221292865993 1106 82-83 2.2913233893296265 1106 84-85 2.0987132103995236 1106 86-87 2.481394197214108 1106 88-89 2.61111346157341 1106 90-91 2.136671313695448 1106 92-93 2.233727196330001 1106 94-95 2.0365919252233624 1106 96-97 2.5921243846348965 1106 98-99 2.1852609448259734 1106 100-101 1.8100772739890054 1107 1 0.08250864505241395 1107 2 0.035521930354235565 1107 3 0.2956412659495413 1107 4 0.12780342876433792 1107 5 0.1288017999536919 1107 6 0.13305042744294582 1107 7 0.14850309284982188 1107 8 0.1970295003822784 1107 9 0.2866496387924897 1107 10-11 0.24528762587749497 1107 12-13 0.2032469905595562 1107 14-15 0.12217206662791114 1107 16-17 0.17943318714818446 1107 18-19 0.16182612078466718 1107 20-21 0.17178948075380163 1107 22-23 0.41892695516891365 1107 24-25 0.025704793547177474 1107 26-27 0.23095553762125576 1107 28-29 0.20473080489488638 1107 30-31 0.3162819806061776 1107 32-33 0.3917874076668042 1107 34-35 0.48992265114951294 1107 36-37 0.5005086154349385 1107 38-39 0.6194853084526741 1107 40-41 0.7949878480193533 1107 42-43 0.7268901958743754 1107 44-45 0.5724384023779336 1107 46-47 0.7616495088262312 1107 48-49 0.7458392513569905 1107 50-51 0.3909310711305878 1107 52-53 0.23596783989502512 1107 54-55 0.29298512717324243 1107 56-57 0.27679921644531547 1107 58-59 0.38737384375533424 1107 60-61 0.7919602363010583 1107 62-63 0.41665413160317755 1107 64-65 0.46103767909362503 1107 66-67 0.8446654491759595 1107 68-69 0.8691589787444869 1107 70-71 1.0272068734800825 1107 72-73 0.838389735504478 1107 74-75 0.7144394279540336 1107 76-77 0.4509276419526884 1107 78-79 0.3988501078504285 1107 80-81 0.6788219040141819 1107 82-83 0.7738816650019444 1107 84-85 0.9577247191862384 1107 86-87 0.7332411057643284 1107 88-89 0.7401921732449352 1107 90-91 0.8433699427097672 1107 92-93 1.0079477482366173 1107 94-95 0.7736723462365553 1107 96-97 0.8337503666282657 1107 98-99 1.1388628555421434 1107 100-101 1.7536836336766406 1108 1 -0.09588282067037213 1108 2 -0.03303250270133873 1108 3 -0.3741470977404511 1108 4 -0.057347617127064154 1108 5 -0.07087619822736713 1108 6 -0.10717276160407607 1108 7 -0.16492020435048715 1108 8 -0.21605458879501782 1108 9 0.055336805422285806 1108 10-11 -0.043149804990441964 1108 12-13 0.19331040771168517 1108 14-15 0.30347629689607913 1108 16-17 0.009356005032806536 1108 18-19 0.06000226315371293 1108 20-21 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46-47 0.17228653358917967 2316 48-49 -0.07736796580216776 2316 50-51 0.5957317707531615 2316 52-53 0.17981392292917775 2316 54-55 0.1623582285264611 2316 56-57 -0.2880079270693656 2316 58-59 -1.2141174569903157 2316 60-61 -0.7763680339741867 2316 62-63 -1.492302878430884 2316 64-65 -1.2409323107802592 2316 66-67 -1.2654100364173395 2316 68-69 -1.288762405771756 2316 70-71 -1.2354737856380282 2316 72-73 -1.2729745285109892 2316 74-75 -1.0587815975453658 2316 76-77 -1.2907873539048111 2316 78-79 -1.0016838146387563 2316 80-81 -0.862603116698331 2316 82-83 -0.4794783644558436 2316 84-85 -0.6851685883715248 2316 86-87 -1.0875266379528767 2316 88-89 -1.0161826771647107 2316 90-91 -0.8257592112992533 2316 92-93 -0.595384664540699 2316 94-95 -0.44604136084252133 2316 96-97 -0.34364077762285916 2316 98-99 -0.5718984442829509 2316 100-101 -0.01675270987486499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 14.0 6 30.0 7 92.0 8 190.0 9 359.0 10 779.0 11 1368.0 12 2300.0 13 3274.0 14 4122.0 15 6276.0 16 8673.0 17 12493.0 18 9616.0 19 4388.0 20 3085.0 21 2807.0 22 2808.0 23 3074.0 24 3352.0 25 3582.0 26 3866.0 27 4414.0 28 4991.0 29 6165.0 30 7323.0 31 8595.0 32 10874.0 33 13951.0 34 19006.0 35 26433.0 36 37848.0 37 61009.0 38 46025.0 39 3350.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72671682760649 22.442220969560317 16.13462575363953 17.696436449193666 2 15.746550905722204 23.85961688639809 40.26857764098795 20.125254566891755 3 18.516544933927452 27.94524323579402 34.98430489840293 18.553906931875602 4 10.930221875143554 21.08135421930268 39.32534031573951 28.663083589814264 5 9.39916515837797 41.871319239638744 34.84162203050877 13.88789357147451 6 23.864516820555224 42.11401534291883 18.78144762429755 15.240020212228398 7 22.749169308037423 41.3744315310763 19.74336594852007 16.13303321236621 8 26.366851945426983 34.1525410752293 20.0921800113311 19.388426968012617 9 27.467193409588557 16.03074708683602 22.31583138101582 34.186228122559605 10-11 23.30531183487222 29.157058202030413 27.88521904236912 19.652410920728254 12-13 25.186057480276375 29.846529377174498 28.296203508599987 16.671209633949136 14-15 21.833187866538044 28.905170961765204 31.34120385257323 17.920437319123526 16-17 16.218169568346426 32.62912092118762 31.292817002771528 19.859892507694425 18-19 16.17927634097417 28.919258272466962 38.75771356822393 16.14375181833494 20-21 17.583134400806042 26.84524912375262 39.53870111733982 16.03291535810152 22-23 17.643419873978853 27.427132061892767 38.9950464348878 15.934401629240579 24-25 17.1062826343271 27.741589722388106 39.796958978363726 15.355168664921065 26-27 16.650996845619083 28.01580252962974 40.39598199246623 14.937218632284935 28-29 15.8079458536548 29.066909631036147 39.897556829927495 15.227587685381557 30-31 16.92547035611857 27.67961731455635 40.18074659798582 15.21416573133926 32-33 15.971359840996893 28.245254329940632 40.13664712304519 15.646738706017283 34-35 16.34863031528014 28.88725557750318 39.003475890792714 15.760638216423967 36-37 17.04947402269282 28.253939087693507 38.510266893288616 16.18631999632505 38-39 15.686832958182123 29.85637068001899 38.351325279066565 16.105471082732326 40-41 16.4680662103603 29.631739323502842 37.85597256036872 16.04422190576814 42-43 16.287705929735043 29.18574486917585 37.69444452952004 16.832104671569063 44-45 17.44499058293904 29.585802440779702 36.49287212703079 16.476334849250463 46-47 17.68018742248151 29.90399191510864 35.36803099208355 17.047789670326306 48-49 17.451421746520282 30.12157961627391 35.162999372195934 17.26399926500988 50-51 17.456321680677416 30.549711363253557 33.64800097998683 18.345965976082194 52-53 18.14491555269726 31.64545913914282 32.45578575037898 17.753839557780942 54-55 18.3738343516009 30.254796576171007 32.28474742370649 19.0866216485216 56-57 18.817584638706418 31.758004501814508 31.67577748174009 17.748633377738987 58-59 19.118164974658153 31.40612798015527 31.231720948749754 18.243986096436828 60-61 18.43278668442893 30.352029644601654 32.90627344695056 18.308910224018867 62-63 18.348722189045585 30.063392898157932 32.78882815012174 18.799056762674752 64-65 17.844641462630346 30.207328464023764 32.93046687185141 19.017563201494482 66-67 18.12470332429908 31.147503330423998 31.482995697245318 19.244797648031607 68-69 18.286094905599708 29.87673603135958 31.98432021069717 19.852848852343545 70-71 18.095150596413863 30.563186182185675 32.635705207711275 18.70595801368919 72-73 18.147824888603058 29.901082579202843 33.355995528810084 18.595097003384016 74-75 17.532730028940236 31.279801552666637 32.09395623746306 19.09351218093007 76-77 17.323717212549955 30.73759321359119 32.39821152403265 19.540478049826206 78-79 17.164469352443078 31.259283078383632 31.345797540845545 20.230450028327745 80-81 16.963878297885373 29.868008023642183 32.17955196227051 20.98856171620194 82-83 16.31093382741381 30.82985613989419 32.01917113916685 20.840038893525147 84-85 16.49241275820356 30.484634112729108 31.55036979190592 21.472583337161407 86-87 15.989403892385196 30.45171268011086 31.331710230143784 22.227173197360163 88-89 16.410951352841195 29.973662853905402 31.64607163091246 21.969314162340943 90-91 16.243880252798967 28.768669344526643 32.76570460744033 22.221745795234064 92-93 15.851247743312626 28.325158982572784 33.0836876913112 22.73990558280339 94-95 15.65207405025495 28.403846448313352 33.0401028986173 22.9039766028144 96-97 16.675394674384066 28.231736261043995 31.888159002863397 23.204710061708546 98-99 16.449385211386222 28.387615416417844 31.356056777987046 23.80694259420889 100-101 15.96748555134594 27.37372933007862 32.61460148667232 24.044183631903117 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1067.0 1 696.5 2 381.0 3 424.5 4 342.0 5 371.5 6 629.5 7 1059.5 8 1442.5 9 1552.5 10 1357.0 11 1106.5 12 1027.5 13 990.0 14 1007.5 15 1065.5 16 1180.0 17 1372.0 18 1551.5 19 1808.0 20 2149.5 21 2418.5 22 2705.5 23 3111.0 24 3554.5 25 4310.0 26 6038.0 27 8720.5 28 9447.0 29 9452.0 30 10154.0 31 10591.0 32 10899.0 33 10947.0 34 12582.0 35 14558.5 36 15703.0 37 16098.5 38 13637.5 39 10705.0 40 9799.0 41 9109.0 42 8414.5 43 8206.0 44 8088.0 45 7777.5 46 7402.5 47 7416.5 48 8386.0 49 8191.0 50 6939.5 51 6016.0 52 5160.5 53 4444.0 54 3772.0 55 3258.5 56 2754.5 57 2334.5 58 2042.0 59 1812.5 60 1654.5 61 1482.5 62 1200.5 63 853.5 64 558.0 65 390.5 66 293.0 67 233.5 68 172.0 69 141.5 70 135.0 71 126.0 72 98.5 73 61.0 74 36.5 75 24.0 76 13.0 77 11.5 78 12.0 79 9.0 80 8.5 81 8.0 82 3.5 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03644326029368981 2 0.0 3 0.0 4 0.0 5 0.0018374753089255363 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007043655350881223 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.5312294241046136E-4 22-23 7.656147120523067E-4 24-25 0.0 26-27 0.0015312294241046135 28-29 0.0038280735602615338 30-31 1.5312294241046136E-4 32-33 1.5312294241046136E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.008574884774985836 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.656147120523067E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.009340499487038143 92-93 0.001684352366515075 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.010412360083911373 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 326535.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.62680347678004 #Duplication Level Percentage of deduplicated Percentage of total 1 77.33008893350008 33.73664592743731 2 12.358719752252096 10.783428757122039 3 4.222379351879459 5.526267425665771 4 1.9652604239358864 3.429521211829774 5 1.0426097499853861 2.274286533279361 6 0.5949531903596886 1.5573543548223263 7 0.3923261941968518 1.1981156441113368 8 0.2839053291413742 0.9908705600369028 9 0.19883779649955907 0.7807191724478022 >10 1.2680825266810256 10.969124270315332 >50 0.1847665279970216 5.534961966372222 >100 0.1376967280890351 12.203949032449701 >500 0.01194308355874284 3.4428389040511203 >1k 0.007727877596833603 5.816322788407202 >5k 7.025343269848729E-4 1.755593451651813 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5728 1.7541764282542454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4750 1.4546679528993829 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3438 1.0528733520143323 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1802 0.5518550844473028 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1450 0.4440565329903379 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 1207 0.3696387829788537 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1100 0.33687047330301495 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1075 0.3292143261824919 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1066 0.3264581132191036 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1047 0.32063944140750605 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1041 0.3188019660985805 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1001 0.3065521307057436 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 922 0.28235870580489075 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 921 0.28205245992006983 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 803 0.24591544551120095 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 791 0.24224049489334987 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 788 0.2413217572388871 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 709 0.2171283323380342 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 689 0.21100341464161576 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 676 0.20702221813894378 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 642 0.1966098580550324 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 567 0.1736414166934632 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 562 0.17211018726935856 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 555 0.1699664660756121 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 537 0.1644540401488355 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 528 0.1616978271854472 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 522 0.15986035187652167 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 516 0.15802287656759612 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 505 0.15465417183456598 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 497 0.15220420475599858 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 491 0.15036672944707305 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 467 0.1430168282113709 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 466 0.14271058232654998 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 459 0.1405668611328035 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 440 0.134748189321206 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 440 0.134748189321206 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 437 0.13382945166674323 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 433 0.13260446812745952 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 426 0.13046074693371307 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 413 0.1264795504310411 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 407 0.12464207512211554 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 400 0.12249835392836908 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 391 0.11974214096498077 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 384 0.11759841977123432 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 381 0.11667968211677156 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 375 0.11484220680784603 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 375 0.11484220680784603 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 374 0.11453596092302509 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 358 0.10963602676589033 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 342 0.10473609260875556 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 337 0.10320486318465096 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 337 0.10320486318465096 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 336 0.10289861729983003 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 335 0.1025923714150091 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 333 0.10197987964536727 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.5312294241046136E-4 0.0 16-17 0.0 0.0 0.0 3.062458848209227E-4 0.0 18-19 0.0 0.0 0.0 3.062458848209227E-4 0.0 20-21 0.0 0.0 0.0 3.062458848209227E-4 0.0 22-23 0.0 0.0 0.0 6.124917696418454E-4 0.0 24-25 0.0 0.0 0.0 0.0019905982513359977 0.0 26-27 0.0 0.0 0.0 0.004746811214724303 0.0 28-29 0.0 0.0 0.0 0.014240433644172906 0.0 30-31 0.0 0.0 0.0 0.02786837551870397 0.0 32-33 0.0 0.0 0.0 0.04394628447180241 0.0 34-35 0.0 0.0 0.0 0.06109605402177408 0.0 36-37 0.0 0.0 0.0 0.08115515947754452 0.0 38-39 0.0 0.0 0.0 0.11300473149892049 0.0 40-41 0.0 0.0 0.0 0.17915384262023978 0.0 42-43 0.0 0.0 0.0 0.24744667493530553 0.0 44-45 0.0 0.0 0.0 0.31191143369010976 0.0 46-47 0.0 0.0 0.0 0.4365535088122253 0.0 48-49 0.0 0.0 0.0 0.524752323640651 0.0 50-51 0.0 0.0 0.0 0.6448007104904527 0.0 52-53 0.0 0.0 0.0 0.7804676374661216 0.0 54-55 0.0 0.0 0.0 0.9426248334788001 0.0 56-57 0.0 0.0 0.0 1.3013918875465111 0.0 58-59 0.0 0.0 0.0 1.6491340897606688 0.0 60-61 0.0 0.0 0.0 1.9576768187177485 0.0 62-63 0.0 0.0 0.0 2.2894942349212184 0.0 64-65 0.0 0.0 0.0 2.6009462997840966 0.0 66-67 0.0 0.0 0.0 3.0587838975913764 0.0 68-69 0.0 0.0 0.0 3.6562696188769968 0.0 70-71 0.0 0.0 0.0 4.077357710505765 0.0 72-73 0.0 0.0 0.0 4.46736184482521 0.0 74-75 0.0 0.0 0.0 5.046472813021575 0.0 76-77 0.0 0.0 0.0 5.763547552329766 0.0 78-79 0.0 0.0 0.0 6.46209441560629 0.0 80-81 0.0 0.0 0.0 7.1660005818671815 0.0 82-83 0.0 0.0 0.0 7.941262039291347 0.0 84-85 0.0 0.0 0.0 8.769657157731944 0.0 86-87 0.0 0.0 0.0 9.642764175356394 0.0 88-89 0.0 0.0 0.0 10.817982758356685 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAT 25 0.0050197947 56.995037 9 GGTATCA 2975 0.0 43.278397 1 GTATCAA 4755 0.0 34.571198 1 TAGACAG 110 2.7241476E-6 30.22927 5 GTAATGG 100 4.9350143E-5 28.50625 1 TAATGGG 100 4.9401722E-5 28.501884 2 ATAGGGT 85 6.3948095E-4 27.943024 3 ATCAACG 6105 0.0 26.844526 3 TATCAAC 6110 0.0 26.822561 2 TCAACGC 6200 0.0 26.433197 4 GGACTGA 90 8.938318E-4 26.386593 6 CAACGCA 6275 0.0 26.117264 5 AACGCAG 6395 0.0 25.623259 6 TTAGAGG 95 0.001224202 25.001652 3 GTACATG 2960 0.0 24.236732 1 TACATGG 2925 0.0 24.19818 2 CATGGGT 415 0.0 24.037733 4 TCCAGAA 640 0.0 23.751568 2 AGAGTGC 100 0.0016519565 23.747932 8 TTAGGCA 245 2.9467628E-10 23.266842 4 >>END_MODULE