##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937490_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 324075 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.71867006094268 33.0 31.0 34.0 30.0 34.0 2 31.77976085782612 33.0 31.0 34.0 30.0 34.0 3 31.745197870863226 33.0 31.0 34.0 30.0 34.0 4 35.422130679626626 37.0 35.0 37.0 33.0 37.0 5 35.47784309187688 37.0 35.0 37.0 33.0 37.0 6 35.567612435393045 37.0 35.0 37.0 33.0 37.0 7 35.53423744503587 37.0 35.0 37.0 33.0 37.0 8 35.53000385713184 37.0 35.0 37.0 33.0 37.0 9 37.09614441101597 39.0 37.0 39.0 34.0 39.0 10-11 37.00884208902261 39.0 37.0 39.0 33.0 39.0 12-13 36.893813160533824 39.0 37.0 39.0 33.0 39.0 14-15 37.951818251947856 40.0 38.0 41.0 33.0 41.0 16-17 37.97307721977937 40.0 38.0 41.0 33.0 41.0 18-19 38.12584895471727 40.0 38.0 41.0 33.5 41.0 20-21 38.18791329167631 40.0 38.0 41.0 34.0 41.0 22-23 38.04714957957263 40.0 38.0 41.0 33.5 41.0 24-25 37.87770423513076 40.0 38.0 41.0 33.0 41.0 26-27 37.6843431304482 40.0 37.0 41.0 33.0 41.0 28-29 37.62523027077066 40.0 37.0 41.0 33.0 41.0 30-31 37.25071974080075 39.0 36.5 41.0 33.0 41.0 32-33 36.79339813314819 39.0 35.0 41.0 32.0 41.0 34-35 36.50166165239528 39.0 35.0 41.0 31.0 41.0 36-37 36.27972537221322 39.0 35.0 41.0 31.0 41.0 38-39 36.0289176888066 39.0 35.0 41.0 30.0 41.0 40-41 35.66519941371596 39.0 35.0 41.0 29.5 41.0 42-43 35.104335416184526 39.0 35.0 41.0 26.0 41.0 44-45 34.42094268302091 39.0 35.0 41.0 19.0 41.0 46-47 33.44124199645144 38.0 34.0 41.0 2.0 41.0 48-49 32.67682018051377 38.0 33.0 40.0 2.0 41.0 50-51 31.639490858597547 37.5 32.0 39.5 2.0 40.5 52-53 31.61723675075214 38.0 32.5 39.5 2.0 40.5 54-55 31.815462470107228 38.0 33.0 40.0 2.0 41.0 56-57 31.58500964282959 38.0 32.0 40.0 2.0 41.0 58-59 31.33046671295225 38.0 31.5 40.0 2.0 41.0 60-61 31.043827817634806 38.0 31.0 40.0 2.0 41.0 62-63 30.69209905114557 37.0 31.0 40.0 2.0 41.0 64-65 30.32687186608038 37.0 30.0 40.0 2.0 41.0 66-67 29.92552341279025 36.0 29.0 39.0 2.0 41.0 68-69 29.40073748360719 35.5 28.5 39.0 2.0 40.5 70-71 28.95950782997763 35.0 28.0 38.5 2.0 40.0 72-73 28.56594615443956 35.0 26.5 37.5 2.0 40.0 74-75 28.116477667206667 35.0 26.0 37.0 2.0 39.0 76-77 27.679672915220245 35.0 26.0 36.5 2.0 39.0 78-79 27.21328396204582 34.0 24.5 36.0 2.0 39.0 80-81 26.729309573401217 34.0 22.0 36.0 2.0 37.5 82-83 26.27407235979326 34.0 20.0 35.0 2.0 37.0 84-85 25.864534444187303 34.0 17.5 35.0 2.0 36.5 86-87 25.45299544858443 34.0 9.0 35.0 2.0 36.0 88-89 25.03872251793566 33.0 2.0 35.0 2.0 36.0 90-91 24.63384710329399 33.0 2.0 35.0 2.0 35.0 92-93 24.24381238910746 33.0 2.0 35.0 2.0 35.0 94-95 23.776827894777444 32.5 2.0 35.0 2.0 35.0 96-97 23.2361058396976 32.0 2.0 35.0 2.0 35.0 98-99 22.602545707012265 31.5 2.0 34.5 2.0 35.0 100-101 21.115339041888454 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.14085800780479119 1101 2 -0.07987688675531857 1101 3 -0.03869216220870797 1101 4 -0.18218553756221212 1101 5 -0.11592448761314245 1101 6 -0.2958767591365046 1101 7 -0.2700881222818552 1101 8 -0.16861778508883418 1101 9 -0.19907921072257295 1101 10-11 -0.16934658093664012 1101 12-13 -0.2894793018884414 1101 14-15 -0.23367215067942482 1101 16-17 -0.05282354253252919 1101 18-19 0.03549003588140209 1101 20-21 0.04068755665119994 1101 22-23 -0.11709267793008138 1101 24-25 -0.23482301384976267 1101 26-27 0.042128215473077546 1101 28-29 -0.15066217472276122 1101 30-31 0.17708976703247714 1101 32-33 0.38312185653347086 1101 34-35 0.2616843946214189 1101 36-37 0.2910576049136111 1101 38-39 0.14362872066870835 1101 40-41 -0.24326535278304817 1101 42-43 0.045360453831108316 1101 44-45 0.44090515070929115 1101 46-47 0.7831198919218494 1101 48-49 0.801945668743663 1101 50-51 0.7111466042251671 1101 52-53 0.6320461414552732 1101 54-55 0.8406359116879223 1101 56-57 1.1097259671793047 1101 58-59 1.0616773838061597 1101 60-61 1.1101773199648157 1101 62-63 0.9833897244270986 1101 64-65 1.0347392052065345 1101 66-67 1.2628580957077666 1101 68-69 1.1329691694027346 1101 70-71 1.185154831668953 1101 72-73 1.375992204231327 1101 74-75 1.1022284873044867 1101 76-77 0.8700956334531647 1101 78-79 1.1600642133166161 1101 80-81 1.2259305898206847 1101 82-83 1.0223993296938438 1101 84-85 0.9979121521409873 1101 86-87 1.239977972188946 1101 88-89 1.09767898910507 1101 90-91 1.117951801712863 1101 92-93 1.1930944705191493 1101 94-95 1.1962422369189518 1101 96-97 1.413107105452415 1101 98-99 1.2518102714807924 1101 100-101 1.21810989300214 1102 1 0.10937555310070479 1102 2 -0.023157519166385754 1102 3 0.023255376418671148 1102 4 0.059747628086363136 1102 5 0.1709781354554849 1102 6 0.09580487938307414 1102 7 2.316764964405138E-4 1102 8 0.09739012004951064 1102 9 -0.11235624988893989 1102 10-11 0.10280577263863933 1102 12-13 0.018165326210734634 1102 14-15 0.1127256215088579 1102 16-17 0.28726808520732305 1102 18-19 0.06192733376138193 1102 20-21 0.1316774955660449 1102 22-23 -0.0386251967946194 1102 24-25 0.08411338571904992 1102 26-27 0.09596400419028583 1102 28-29 -0.0893704032531204 1102 30-31 0.19935890106051346 1102 32-33 0.10301944905952354 1102 34-35 0.06348101697140152 1102 36-37 0.16392860742887905 1102 38-39 0.0184760077689603 1102 40-41 0.2364597639974022 1102 42-43 0.17544489508155436 1102 44-45 0.5319489882946229 1102 46-47 0.4487415232549381 1102 48-49 0.48879439314179507 1102 50-51 0.5754477181310271 1102 52-53 0.47406015510960486 1102 54-55 0.12496038168432833 1102 56-57 0.44448162653970513 1102 58-59 0.7423636935438545 1102 60-61 0.7941694148264773 1102 62-63 0.6166362210135148 1102 64-65 0.8895721193279869 1102 66-67 1.2879389437135167 1102 68-69 1.3906227806136577 1102 70-71 1.0458177853160961 1102 72-73 0.9971204830671141 1102 74-75 0.7216948904665443 1102 76-77 0.8145889859504685 1102 78-79 1.0596689563996229 1102 80-81 1.198262605630962 1102 82-83 1.163541981508434 1102 84-85 1.1509574270242062 1102 86-87 0.888989829896456 1102 88-89 0.8955949071790918 1102 90-91 0.9529410219787664 1102 92-93 1.1130279954921996 1102 94-95 0.9184395060529766 1102 96-97 0.8319446907919783 1102 98-99 0.9717887120125894 1102 100-101 1.3008892677775634 1103 1 -0.06939347691234588 1103 2 0.16071807891973933 1103 3 0.12339021765399139 1103 4 0.17557189950872498 1103 5 0.1428398144463543 1103 6 0.14329944223648994 1103 7 0.3210102758874811 1103 8 0.2934188281834835 1103 9 0.08483383275568457 1103 10-11 0.2631820231823454 1103 12-13 0.16236280337471953 1103 14-15 0.14048116739837724 1103 16-17 0.21336638881758319 1103 18-19 0.06430010453128432 1103 20-21 -0.014655692776713636 1103 22-23 -0.21804233468065348 1103 24-25 -0.13092141201909868 1103 26-27 0.0554920598666655 1103 28-29 -0.2393921518394606 1103 30-31 0.1753506365976989 1103 32-33 0.20171453387900584 1103 34-35 0.4496706646443087 1103 36-37 0.5093070328312308 1103 38-39 0.3278735719272916 1103 40-41 0.7407964321139744 1103 42-43 1.0112300190484973 1103 44-45 1.2219120156978462 1103 46-47 0.9905684045076484 1103 48-49 1.6648861721761605 1103 50-51 1.5834120504494216 1103 52-53 1.873144539624608 1103 54-55 1.995258337317221 1103 56-57 2.0462019397193494 1103 58-59 2.1170091915864795 1103 60-61 2.2184038645872413 1103 62-63 2.079167756463711 1103 64-65 2.167027534725989 1103 66-67 2.1233043199640598 1103 68-69 2.3173169954896906 1103 70-71 2.6062875547810798 1103 72-73 2.796816002858325 1103 74-75 2.570283407212955 1103 76-77 2.590930873727764 1103 78-79 2.528233549701053 1103 80-81 2.082257820941969 1103 82-83 2.353074386902083 1103 84-85 2.4397199324613545 1103 86-87 2.6043486815711 1103 88-89 2.241351757983793 1103 90-91 2.3319449367243053 1103 92-93 2.4103942416861948 1103 94-95 2.4536678662095674 1103 96-97 2.3504984098002417 1103 98-99 2.4450596993984135 1103 100-101 2.387537810622277 1104 1 -0.06984807252086256 1104 2 -0.23923337203468975 1104 3 -0.1672993994705827 1104 4 -0.25026501983365534 1104 5 -0.27476142134753445 1104 6 -0.2913557492012231 1104 7 0.03670706503014998 1104 8 0.015581467486136091 1104 9 0.07577722898210482 1104 10-11 -0.028163006057418727 1104 12-13 -0.016785381553006573 1104 14-15 0.10592086727700689 1104 16-17 -0.1907713139298508 1104 18-19 0.11908770245691613 1104 20-21 0.22169430170634996 1104 22-23 0.37924995264142325 1104 24-25 0.30319311959294737 1104 26-27 0.051689786888623246 1104 28-29 -6.963557454540137E-4 1104 30-31 0.28092715651384026 1104 32-33 0.20019185926795302 1104 34-35 0.7692999264781335 1104 36-37 0.5561678956801828 1104 38-39 0.5958687170227321 1104 40-41 0.41219221717411614 1104 42-43 1.1987352601386476 1104 44-45 1.4935439256614345 1104 46-47 1.8533710085019166 1104 48-49 2.38059620196762 1104 50-51 2.1456739946180257 1104 52-53 1.9773109304444283 1104 54-55 1.934706643617556 1104 56-57 2.0570416175302277 1104 58-59 1.647603917394715 1104 60-61 2.0004539583751715 1104 62-63 1.8006374684796 1104 64-65 1.8184189062365235 1104 66-67 2.086003205542788 1104 68-69 2.176729993393252 1104 70-71 2.2621145436368657 1104 72-73 2.472309768719523 1104 74-75 2.2747013703598142 1104 76-77 2.0768384098637007 1104 78-79 2.0877326118217674 1104 80-81 2.2567960533153553 1104 82-83 2.0021618855228454 1104 84-85 2.1203576903781567 1104 86-87 2.0448407923511915 1104 88-89 1.8443816597823144 1104 90-91 1.83674908546093 1104 92-93 2.0801239117733665 1104 94-95 2.2950139988367333 1104 96-97 1.9397227284585234 1104 98-99 2.03762286832702 1104 100-101 2.1942287518117283 1105 1 0.08907000204649052 1105 2 -0.08064415018242599 1105 3 -0.10477627701405012 1105 4 -0.08152247040298732 1105 5 -0.014967776426253465 1105 6 0.09023672007160144 1105 7 -0.15632947872781955 1105 8 0.20458956548424823 1105 9 0.149234704215381 1105 10-11 -0.15234601259336245 1105 12-13 -0.1314727659407282 1105 14-15 0.23608488256866167 1105 16-17 0.1800101223343873 1105 18-19 -0.05785561155632024 1105 20-21 0.24043890646649402 1105 22-23 0.11361917202729188 1105 24-25 0.2959172532715755 1105 26-27 0.14810529229414016 1105 28-29 0.08770336472974094 1105 30-31 0.1451109851815815 1105 32-33 0.1130664430637296 1105 34-35 0.2204652982872375 1105 36-37 0.239544390932366 1105 38-39 0.33077232207062224 1105 40-41 0.4454885545769969 1105 42-43 0.6244762064123321 1105 44-45 1.141198794736944 1105 46-47 1.3381615704290013 1105 48-49 1.2615104125155696 1105 50-51 1.3490077390172495 1105 52-53 1.258767747972275 1105 54-55 1.6871216557440043 1105 56-57 1.730593637919096 1105 58-59 1.6458774406404864 1105 60-61 1.833211799457093 1105 62-63 1.8283433097259518 1105 64-65 1.8419524286601678 1105 66-67 1.8233937592485496 1105 68-69 2.2617178669493825 1105 70-71 1.877233547213141 1105 72-73 1.656981121537381 1105 74-75 1.7390002974778334 1105 76-77 2.025085213826376 1105 78-79 2.023770758736717 1105 80-81 2.3741142594237914 1105 82-83 2.15499618486103 1105 84-85 2.2189929516105344 1105 86-87 2.269015577569263 1105 88-89 2.449059589654471 1105 90-91 2.292004562913963 1105 92-93 2.1191340651009405 1105 94-95 2.4436965326469 1105 96-97 2.5379058172076476 1105 98-99 2.837824290615014 1105 100-101 2.9804950402977752 1106 1 -0.04186982908309389 1106 2 -0.03645873408050093 1106 3 -0.02635996425238929 1106 4 -0.1077315203311997 1106 5 -0.15781989935427987 1106 6 0.014902643788758496 1106 7 -0.18115804814841852 1106 8 -0.0952156612659465 1106 9 -0.11894496908762875 1106 10-11 -0.5152341786124666 1106 12-13 -0.44696778115111613 1106 14-15 -0.3579107802125847 1106 16-17 -0.44882133856538786 1106 18-19 -0.16724488697975204 1106 20-21 -0.06914514220674306 1106 22-23 -0.47581337518514033 1106 24-25 -0.3538222124071666 1106 26-27 -0.4469721652121592 1106 28-29 -0.4118272718976712 1106 30-31 -0.3854327735405505 1106 32-33 -0.26628207053526154 1106 34-35 -0.3648333372960266 1106 36-37 -0.3869578228563739 1106 38-39 -0.31519215691093194 1106 40-41 -0.4135668955600451 1106 42-43 -0.5498759717349131 1106 44-45 -0.44549036121217256 1106 46-47 0.05009544792264364 1106 48-49 0.49997712536558225 1106 50-51 0.5584617715263143 1106 52-53 0.60968589300807 1106 54-55 0.6135872240501747 1106 56-57 0.6959812266463423 1106 58-59 0.6615471493842016 1106 60-61 1.0564756569714255 1106 62-63 1.0978507542808345 1106 64-65 1.2082014372241687 1106 66-67 1.50463830007563 1106 68-69 1.2642755208353122 1106 70-71 1.3770151802964783 1106 72-73 1.641710497158595 1106 74-75 1.2536911200434204 1106 76-77 1.573196214499049 1106 78-79 1.3996354415273977 1106 80-81 1.1059396060499012 1106 82-83 1.1800280614109333 1106 84-85 1.149705379951044 1106 86-87 1.1455880703656653 1106 88-89 1.3185916318620343 1106 90-91 1.1977804933578255 1106 92-93 1.1478329998719836 1106 94-95 1.1043518341940448 1106 96-97 1.129346336067524 1106 98-99 1.2552464566140316 1106 100-101 1.0241106944447367 1107 1 0.012162160432765745 1107 2 0.08348583747697091 1107 3 0.24729648682341931 1107 4 0.16358318646737757 1107 5 0.27454880720797803 1107 6 0.342838149143617 1107 7 0.37949635121565706 1107 8 0.3124250850844703 1107 9 0.03205993998179224 1107 10-11 0.32809185019743836 1107 12-13 0.34635985895071997 1107 14-15 0.45903839963982307 1107 16-17 0.3678995749297158 1107 18-19 0.18766901632124444 1107 20-21 0.17999281660863886 1107 22-23 0.5083127137796595 1107 24-25 0.19035941241962462 1107 26-27 0.28443084038439537 1107 28-29 0.45354123056760187 1107 30-31 0.36350914475620755 1107 32-33 0.3443841449696663 1107 34-35 0.6824151131782301 1107 36-37 0.7864386731207631 1107 38-39 0.9032238356986042 1107 40-41 0.8331433834082986 1107 42-43 0.5902043780228539 1107 44-45 0.7648638007548954 1107 46-47 0.5422994804492589 1107 48-49 0.6600965758076214 1107 50-51 0.876941678715891 1107 52-53 0.9295327128548898 1107 54-55 0.8537959400653499 1107 56-57 1.168761134910124 1107 58-59 1.3718932548915959 1107 60-61 1.4436449393020006 1107 62-63 1.3711392936984055 1107 64-65 1.3000934162129205 1107 66-67 1.325473378975225 1107 68-69 1.2494382076109005 1107 70-71 1.469742345604292 1107 72-73 1.424223682897587 1107 74-75 1.2202380140096665 1107 76-77 1.0247500370206133 1107 78-79 1.2898554365860981 1107 80-81 1.3238453562137131 1107 82-83 1.2956409431528506 1107 84-85 1.5451352189292713 1107 86-87 1.4128826037892317 1107 88-89 1.4573399802878022 1107 90-91 1.429366112040217 1107 92-93 1.526701443256556 1107 94-95 1.6998020828700966 1107 96-97 1.9486802279820594 1107 98-99 1.8704430879396945 1107 100-101 1.8765673775465785 1108 1 0.062324681592969 1108 2 -0.017726175567986502 1108 3 -0.049470937554215055 1108 4 -0.061707377570286326 1108 5 -0.004938913115012156 1108 6 0.14392955580347433 1108 7 0.18290794349036332 1108 8 0.1370969329581797 1108 9 0.10116793948422753 1108 10-11 0.4473461457858363 1108 12-13 0.36301489764752404 1108 14-15 0.19425330074798808 1108 16-17 0.2749001536240385 1108 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0.6625895956060539 2202 58-59 0.8174657546809634 2202 60-61 1.1700821567030708 2202 62-63 0.8936699758676419 2202 64-65 1.2246728528405484 2202 66-67 0.9729724662333474 2202 68-69 1.0031804493049492 2202 70-71 1.0641970876493936 2202 72-73 1.1912357872590889 2202 74-75 1.135212597658949 2202 76-77 1.1204404911448016 2202 78-79 1.0390409899885213 2202 80-81 0.9254896958098584 2202 82-83 0.9014066633763989 2202 84-85 0.6985320229284859 2202 86-87 0.6838054560373834 2202 88-89 0.6834612485777427 2202 90-91 1.1223127236615298 2202 92-93 1.2714426458290866 2202 94-95 1.1809855580294801 2202 96-97 0.9136309399207256 2202 98-99 1.2673594246150657 2202 100-101 1.3777047943466485 2203 1 0.45226677126908754 2203 2 0.5771750750686486 2203 3 0.39554254970362734 2203 4 0.5321658233427016 2203 5 0.3742133702871726 2203 6 0.4617761217401295 2203 7 0.5594099335682685 2203 8 0.6796148059327294 2203 9 0.5528826132434901 2203 10-11 0.7032055576282161 2203 12-13 0.7861304542091219 2203 14-15 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1.4738882565623612 2206 92-93 1.661799678354793 2206 94-95 1.6202150032830183 2206 96-97 1.4733555526573845 2206 98-99 2.0856612031578123 2206 100-101 2.294316295295502 2207 1 0.4760316243022302 2207 2 0.301708790994045 2207 3 0.46359979136201446 2207 4 0.3397109802422307 2207 5 0.13544281516080758 2207 6 0.3060905829842895 2207 7 0.3653732194454662 2207 8 0.3007015145373728 2207 9 0.307186773932159 2207 10-11 0.3995644951994919 2207 12-13 0.5237559922292121 2207 14-15 0.6421934798537237 2207 16-17 0.47245188536438576 2207 18-19 0.4976580468985148 2207 20-21 0.548256905460967 2207 22-23 0.3934080106396678 2207 24-25 0.44272469419667004 2207 26-27 0.5208219003048598 2207 28-29 0.4305054428259325 2207 30-31 0.32254520673344445 2207 32-33 0.4019050554317545 2207 34-35 0.8554577568091091 2207 36-37 0.8270255372370414 2207 38-39 0.6734487088646617 2207 40-41 0.7103201282321052 2207 42-43 0.9642616932566455 2207 44-45 1.0015976551358037 2207 46-47 1.2667270137003257 2207 48-49 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-3.6622265593481025 2316 88-89 -3.094467367294193 2316 90-91 -3.722969814943596 2316 92-93 -4.063304794637009 2316 94-95 -4.0437302064368374 2316 96-97 -3.992741026819477 2316 98-99 -4.394117468874384 2316 100-101 -4.45258820865126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1593.0 3 264.0 4 214.0 5 243.0 6 275.0 7 343.0 8 385.0 9 533.0 10 771.0 11 1253.0 12 2180.0 13 3440.0 14 3813.0 15 5700.0 16 7920.0 17 10994.0 18 8029.0 19 3832.0 20 3365.0 21 3002.0 22 3034.0 23 3321.0 24 3423.0 25 3799.0 26 3918.0 27 4080.0 28 4562.0 29 5315.0 30 6643.0 31 7868.0 32 9538.0 33 12629.0 34 17081.0 35 25135.0 36 34959.0 37 57183.0 38 55735.0 39 7701.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.21458169452892 21.899485286375263 15.369249732113547 18.516683286982268 2 15.221624098041461 22.77687147690584 41.489628444158285 20.51187598089442 3 18.171631627169848 27.991048213357107 34.13007948889384 19.707240670579203 4 10.420710170902867 20.223557416986278 40.3322715749004 29.02346083721045 5 9.521347890284257 41.66189448790137 34.840413182976164 13.97634443883821 6 23.885679293366653 41.556461390009154 19.619219635301977 14.938639681322217 7 23.01234140322775 40.762501430192685 19.9886821508861 16.236475015693465 8 26.085275752974326 33.77815438950243 20.458572882100256 19.677996975422992 9 27.53509802708887 16.108912115777105 22.639619024058383 33.716370833075636 10-11 22.989758959750333 28.953749841481937 28.391899958862627 19.664591239905103 12-13 24.733374259501804 29.786467458283916 28.383328720119128 17.096829562095152 14-15 21.57871461352433 28.94392592730039 30.834400262248035 18.642959196927244 16-17 16.59644017398646 32.96078501106357 30.59653604281316 19.846238772136807 18-19 16.325347310805483 29.829997448802096 37.578990498720536 16.265664741671884 20-21 17.733377036608708 27.64036327316918 38.25917691477552 16.367082775446594 22-23 17.749339418874143 28.279520516281448 37.43798113420504 16.53315893063937 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30.886525754879052 34.38138343850946 17.426970757376374 50-51 17.026517963182897 31.26530953087886 33.26699203285827 18.441180473079967 52-53 17.986263651269994 32.03678495189184 32.17832503171116 17.798626365127 54-55 18.071311049532387 30.70321391459251 32.18306200207828 19.042413033796823 56-57 18.286757556035475 32.36785923536059 31.55316316955417 17.79222003904976 58-59 18.82263824261444 31.73017762456135 30.99556325073044 18.451620882093774 60-61 18.099522329916987 30.39635795884926 33.12170539040641 18.38241432082734 62-63 18.130238914603567 30.15790564001719 32.969102191879934 18.74275325349931 64-65 17.618069307313334 30.44828540378768 33.08305778288943 18.85058750600955 66-67 17.88427232732208 31.28422100547536 31.69757828900383 19.13392837819873 68-69 18.062034915894554 29.994311559591054 32.212648741896295 19.731004782618104 70-71 17.68209729582656 30.935051603767167 32.95869843488155 18.424152665524726 72-73 17.83020515936141 30.232213003371655 33.43567339606686 18.50190844120007 74-75 17.28529620473909 31.52034605531876 32.68942679176851 18.50493094817364 76-77 17.377080608404995 30.92905755357866 32.40678039473969 19.287081443276655 78-79 17.277470722374677 31.222973756425613 31.63436787384532 19.865187647354386 80-81 17.210442096149535 29.78471100254327 32.70398342622583 20.300863475081364 82-83 16.700083632591667 30.602572050018782 32.3787984423623 20.318545875027247 84-85 17.179280676072363 30.376727627980888 31.957620267331155 20.486371428615595 86-87 16.999069416576752 30.552506855710103 31.548323867764406 20.90009985994874 88-89 17.323159637469335 30.172654997627134 31.549226538382463 20.954958826521068 90-91 17.38391758442217 28.917955440677233 32.76943673206898 20.928690242831614 92-93 17.57127553173564 28.40107529707478 33.29942756971422 20.728221601475365 94-95 17.349560367535215 28.842455758505125 33.04941691502912 20.758566958930537 96-97 18.39981450709124 28.590640336978783 31.881284538393167 21.12826061753681 98-99 18.371028832032156 28.45203679369251 31.576254154749943 21.600680219525394 100-101 18.984015494505666 27.50073809122653 32.74234510517993 20.772901309087874 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1183.0 1 756.0 2 368.0 3 423.0 4 376.0 5 374.5 6 654.0 7 1149.0 8 1635.5 9 1748.5 10 1443.5 11 1144.5 12 1061.5 13 1087.5 14 1077.5 15 1089.5 16 1210.0 17 1387.0 18 1584.0 19 1820.5 20 2064.5 21 2303.5 22 2582.0 23 2886.5 24 3278.5 25 4127.5 26 5956.0 27 8500.5 28 9215.5 29 9522.0 30 10373.5 31 10862.0 32 11166.5 33 11185.0 34 12808.5 35 14362.5 36 15351.5 37 16027.5 38 13530.5 39 10499.5 40 9513.5 41 8849.0 42 8162.5 43 7917.0 44 7956.0 45 7560.5 46 7084.0 47 7225.0 48 8183.0 49 7958.5 50 6677.0 51 5804.0 52 5065.0 53 4420.5 54 3748.5 55 3224.0 56 2789.5 57 2352.0 58 2011.5 59 1823.5 60 1723.5 61 1615.5 62 1340.5 63 898.0 64 569.0 65 452.0 66 355.0 67 246.0 68 188.5 69 177.0 70 146.0 71 107.5 72 79.0 73 60.0 74 49.5 75 39.0 76 23.5 77 19.5 78 19.5 79 13.5 80 10.5 81 7.0 82 4.0 83 3.5 84 2.0 85 1.5 86 2.0 87 1.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 1.0 98 3.5 99 3.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3631875337499036 2 0.31721052225565066 3 0.31196482295764866 4 0.3165933811617681 5 0.31535909897400294 6 0.22803363418961659 7 0.21353081848337574 8 0.2234050759854972 9 0.21445653012419963 10-11 0.23698218005091415 12-13 0.224793643446733 14-15 0.22124508215690813 16-17 0.2210907968834375 18-19 0.21522795649155288 20-21 0.2104451130139628 22-23 0.21152510992825732 24-25 0.20134228187919465 26-27 0.2022679935200185 28-29 0.21846794723443647 30-31 0.22880506055696984 32-33 0.2218622232507907 34-35 0.23173648075291214 36-37 0.2067422664506673 38-39 0.2041194168016663 40-41 0.20211370824654787 42-43 0.2096736866466096 44-45 0.21306796266296382 46-47 0.21985651469567227 48-49 0.224793643446733 50-51 0.23050219856514698 52-53 0.26074211216539384 54-55 0.22556506981408625 56-57 0.1979480058628404 58-59 0.19748515004242845 60-61 0.1943994445730155 62-63 0.20180513769960656 64-65 0.19486230039342745 66-67 0.19316516238525033 68-69 0.18884517472807222 70-71 0.2002622849649001 72-73 0.19856514695672298 74-75 0.2031937051608424 76-77 0.20735940754454985 78-79 0.20442798734860756 80-81 0.2075136928180205 82-83 0.19640515312813392 84-85 0.19085088328319064 86-87 0.19193088019748517 88-89 0.193319447658721 90-91 0.19779372058936975 92-93 0.19424515929954486 94-95 0.19223945074442644 96-97 0.18899946000154286 98-99 0.2005708555118414 100-101 0.1868394661729538 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 324075.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.553970071089665 #Duplication Level Percentage of deduplicated Percentage of total 1 78.24655835591888 34.86194819155381 2 11.986809968292128 10.681199451502534 3 4.014261309285387 5.3655383469430316 4 1.779789696344377 3.171867874550445 5 1.0116787659470967 2.2537152729781935 6 0.5828605231307308 1.5581250181911748 7 0.3703873880439215 1.1551560021132568 8 0.2544062941148081 0.9067848331110394 9 0.19682800315467427 0.789252206553513 >10 1.2175575847267606 10.730494726792356 >50 0.1774002053712827 5.516354899816243 >100 0.14344469731193563 12.886726208285115 >500 0.012473451940168313 4.196468114305836 >1k 0.004850786865621012 4.184735787323521 >5k 6.929695522315731E-4 1.741633065979931 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5641 1.7406464552958418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4289 1.323459075831212 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3149 0.9716886523181362 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1619 0.4995757154979557 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1206 0.3721360796112011 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1136 0.3505361413253105 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1081 0.3335647612435393 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1074 0.33140476741495023 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 998 0.3079534058474118 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 961 0.2965362956105839 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 941 0.2903648846717581 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 936 0.2888220319370516 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 887 0.2737020751369282 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 823 0.25395356013268533 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 807 0.24901643138162463 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 803 0.24778214919385944 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 788 0.24315359098974004 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 739 0.22803363418961659 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 732 0.22587364036102753 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 706 0.21785080614055385 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 650 0.2005708555118414 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 648 0.1999537144179588 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 564 0.17403378847489007 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 546 0.16847951862994678 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 535 0.16508524261359253 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 528 0.16292524878500347 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 488 0.15058242690735169 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 462 0.14255959268687804 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 452 0.1394738872174651 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 446 0.13762246393581734 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 444 0.13700532284193476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 442 0.13638818174805217 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 442 0.13638818174805217 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 435 0.1342281879194631 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 432 0.13330247627863923 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 430 0.13268533518475661 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 428 0.13206819409087403 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 426 0.13145105299699145 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 424 0.13083391190310886 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 411 0.12682249479287203 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 408 0.12589678315204814 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 403 0.12435393041734166 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 396 0.1221939365887526 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 388 0.11972537221322224 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 386 0.11910823111933966 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 381 0.11756537838463318 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 380 0.1172568078376919 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 377 0.11633109619686802 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 365 0.11262824963357249 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 356 0.10985111471110082 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 343 0.10583969760086398 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 342 0.1055311270539227 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 339 0.10460541541309881 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 336 0.10367970377227492 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.5428527347064721E-4 0.0 16-17 0.0 0.0 0.0 3.0857054694129443E-4 0.0 18-19 0.0 0.0 0.0 3.0857054694129443E-4 0.0 20-21 0.0 0.0 0.0 4.6285582041194164E-4 0.0 22-23 0.0 0.0 0.0 0.0010799969142945304 0.0 24-25 0.0 0.0 0.0 0.0024685643755303554 0.0 26-27 0.0 0.0 0.0 0.0055542698449433 0.0 28-29 0.0 0.0 0.0 0.012342821877651778 0.0 30-31 0.0 0.0 0.0 0.025457070122656793 0.0 32-33 0.0 0.0 0.0 0.03563989817171951 0.0 34-35 0.0 0.0 0.0 0.05183985188613747 0.0 36-37 0.0 0.0 0.0 0.06649695286584895 0.0 38-39 0.0 0.0 0.0 0.09581115482527193 0.0 40-41 0.0 0.0 0.0 0.15305099128288205 0.0 42-43 0.0 0.0 0.0 0.20396513152819562 0.0 44-45 0.0 0.0 0.0 0.25827354778986344 0.0 46-47 0.0 0.0 0.0 0.3597932577335493 0.0 48-49 0.0 0.0 0.0 0.4261359253259276 0.0 50-51 0.0 0.0 0.0 0.5310499112859677 0.0 52-53 0.0 0.0 0.0 0.6466095811154825 0.0 54-55 0.0 0.0 0.0 0.7857748977860064 0.0 56-57 0.0 0.0 0.0 1.127053922703078 0.0 58-59 0.0 0.0 0.0 1.4262130679626628 0.0 60-61 0.0 0.0 0.0 1.6897323150505286 0.0 62-63 0.0 0.0 0.0 2.009102831134768 0.0 64-65 0.0 0.0 0.0 2.3085705469412945 0.0 66-67 0.0 0.0 0.0 2.7597006865694667 0.0 68-69 0.0 0.0 0.0 3.3148191005168552 0.0 70-71 0.0 0.0 0.0 3.665200956568696 0.0 72-73 0.0 0.0 0.0 4.037799892000309 0.0 74-75 0.0 0.0 0.0 4.626398210290828 0.0 76-77 0.0 0.0 0.0 5.336881894623158 0.0 78-79 0.0 0.0 0.0 6.016199953714418 0.0 80-81 0.0 0.0 0.0 6.688575175499499 0.0 82-83 0.0 0.0 0.0 7.432847334721901 0.0 84-85 0.0 0.0 0.0 8.225873640361028 0.0 86-87 0.0 0.0 0.0 9.0607112551107 0.0 88-89 0.0 0.0 0.0 10.237136465324385 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 205 0.0 50.9855 9 ACGTCAA 40 5.377247E-4 47.509212 8 GGCATTC 220 0.0 47.509212 7 TTAGGCA 235 0.0 46.49838 4 TAGGCAT 230 0.0 45.450634 5 TACCTGG 95 3.8198777E-10 45.001755 2 GCATTCC 240 0.0 43.550114 8 AGGCATT 235 0.0 42.455044 6 CGTCAAA 45 9.6056063E-4 42.23041 9 CCTTTAG 250 0.0 39.94485 1 GGTATCA 2680 0.0 38.326614 1 GTATAGT 100 2.989509E-8 38.042717 1 AGACGTG 25 0.0016112993 38.019146 52-53 GACGTCA 50 0.001612558 38.00737 7 GTATCAA 4335 0.0 35.43194 1 CTTTAGG 300 0.0 33.2513 2 TATAGTA 130 8.245479E-9 32.885902 2 AGCACAA 90 2.4018977E-5 31.677715 5 GAACGAT 30 0.0039549153 31.67281 7 CTACAGT 105 1.8932114E-6 31.672808 4 >>END_MODULE