##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937490_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 324075 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01018282804906 31.0 31.0 34.0 30.0 34.0 2 32.214441101596854 33.0 31.0 34.0 30.0 34.0 3 32.21460464398673 34.0 31.0 34.0 30.0 34.0 4 35.937008408547406 37.0 35.0 37.0 35.0 37.0 5 35.81713492247165 37.0 35.0 37.0 35.0 37.0 6 35.93473424361645 37.0 35.0 37.0 35.0 37.0 7 35.89071665509527 37.0 35.0 37.0 35.0 37.0 8 35.89912520249942 37.0 35.0 37.0 35.0 37.0 9 37.496789323459076 39.0 37.0 39.0 35.0 39.0 10-11 37.449520944225874 39.0 37.0 39.0 34.0 39.0 12-13 37.36825426213068 39.0 37.0 39.0 34.0 39.0 14-15 38.388493404304555 40.0 38.0 41.0 33.5 41.0 16-17 38.409743115019666 40.0 38.0 41.0 33.5 41.0 18-19 38.62091491167168 40.0 38.0 41.0 34.0 41.0 20-21 38.648919231659335 40.0 38.0 41.0 34.0 41.0 22-23 38.51808994831443 40.0 38.0 41.0 34.0 41.0 24-25 38.34633495332871 40.0 38.0 41.0 34.0 41.0 26-27 38.07586206896552 40.0 37.0 41.0 33.5 41.0 28-29 37.93004705700841 40.0 37.0 41.0 33.0 41.0 30-31 37.676275553498414 39.0 36.5 41.0 33.0 41.0 32-33 37.296091954022984 39.0 35.0 41.0 33.0 41.0 34-35 37.00639975314356 39.0 35.0 41.0 33.0 41.0 36-37 36.66045822726221 39.0 35.0 41.0 32.5 41.0 38-39 36.330691969451514 39.0 35.0 41.0 31.0 41.0 40-41 35.952589678315206 39.0 35.0 41.0 30.5 41.0 42-43 35.40544164159531 39.0 35.0 41.0 28.5 41.0 44-45 34.564636272467794 39.0 35.0 41.0 22.0 41.0 46-47 33.49449818714804 38.0 34.0 40.5 2.0 41.0 48-49 32.63044202730849 38.0 33.0 40.0 2.0 41.0 50-51 32.15040191313739 38.0 33.0 40.0 2.0 41.0 52-53 31.84955180128057 38.0 33.0 40.0 2.0 41.0 54-55 31.57300779140631 38.0 32.0 40.0 2.0 41.0 56-57 31.266230039342744 38.0 31.5 40.0 2.0 41.0 58-59 30.99392887448893 38.0 31.0 40.0 2.0 41.0 60-61 30.663729075059784 37.0 31.0 40.0 2.0 41.0 62-63 30.272801049139858 36.5 29.5 40.0 2.0 41.0 64-65 29.921519709943688 36.0 29.0 39.0 2.0 41.0 66-67 29.49424361644681 36.0 28.5 39.0 2.0 40.5 68-69 28.981820566226954 35.0 27.5 38.5 2.0 40.0 70-71 28.607069351230425 35.0 26.0 38.0 2.0 40.0 72-73 28.184614672529506 35.0 26.0 37.0 2.0 39.5 74-75 27.738392347450436 34.5 26.0 37.0 2.0 39.0 76-77 26.47778292062023 33.0 23.0 35.5 2.0 37.5 78-79 26.939473887217467 34.0 24.0 36.0 2.0 38.0 80-81 26.7154701843709 34.0 23.5 35.5 2.0 37.0 82-83 26.34970608655404 34.0 20.5 35.0 2.0 37.0 84-85 26.010246085011186 34.0 19.0 35.0 2.0 36.5 86-87 25.65482064336959 34.0 15.5 35.0 2.0 36.0 88-89 25.268502661420968 33.5 7.0 35.0 2.0 36.0 90-91 24.83898326004783 33.0 2.0 35.0 2.0 35.0 92-93 24.443886446038725 33.0 2.0 35.0 2.0 35.0 94-95 24.059125202499423 32.5 2.0 35.0 2.0 35.0 96-97 23.65481755766412 32.0 2.0 35.0 2.0 35.0 98-99 23.091765794954874 32.0 2.0 34.0 2.0 35.0 100-101 21.874127902491708 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.09745146144392791 1101 2 0.018470709725285417 1101 3 0.016519177740995872 1101 4 0.15300815648066646 1101 5 0.002673706409510146 1101 6 -0.02152935054880345 1101 7 0.0355464197714781 1101 8 -0.017321784985284694 1101 9 0.13542335141423223 1101 10-11 0.017329970751809753 1101 12-13 0.015089452941857928 1101 14-15 -0.03453932095577983 1101 16-17 0.033390800093656026 1101 18-19 0.24019834954982144 1101 20-21 0.0011290659087990207 1101 22-23 0.05301419879968705 1101 24-25 0.05109205850560272 1101 26-27 -0.19677735286440878 1101 28-29 0.18769549458406232 1101 30-31 0.21390882559330038 1101 32-33 -0.05949280785740285 1101 34-35 0.27738305936465935 1101 36-37 0.3755328854954456 1101 38-39 0.16864451470448927 1101 40-41 -0.017721965530220984 1101 42-43 0.6144485788686254 1101 44-45 0.7861561598138209 1101 46-47 1.2841303837751212 1101 48-49 1.2704309003647367 1101 50-51 1.289307917793625 1101 52-53 1.5208915494374118 1101 54-55 1.4347436554289104 1101 56-57 1.5546802743590966 1101 58-59 1.5572845478853274 1101 60-61 1.9820882240604973 1101 62-63 1.6534549983399955 1101 64-65 1.5092060422136626 1101 66-67 1.5140240174357906 1101 68-69 1.8283462065040794 1101 70-71 1.6206463410815921 1101 72-73 1.5682912638329256 1101 74-75 1.6608630731981648 1101 76-77 1.7261156921333551 1101 78-79 1.6851256276050002 1101 80-81 1.4681064104822639 1101 82-83 1.282529299388301 1101 84-85 1.3695073830666011 1101 86-87 1.0463324658853494 1101 88-89 1.1355218858505083 1101 90-91 1.4025326784418475 1101 92-93 1.633805720225883 1101 94-95 1.4024006406915426 1101 96-97 1.1160292731584498 1101 98-99 0.8588706101859387 1101 100-101 0.855243375547019 1102 1 0.18914573582520333 1102 2 0.1573136849318928 1102 3 0.2904142549957598 1102 4 0.07974189704840029 1102 5 0.049026563039042514 1102 6 0.058905432059894736 1102 7 -0.026580781090899563 1102 8 0.2149994510908897 1102 9 0.12351174523384856 1102 10-11 0.18010395566017934 1102 12-13 0.27787242096197673 1102 14-15 0.30831012209129227 1102 16-17 0.22262903221727015 1102 18-19 0.36611462694831687 1102 20-21 0.3880406720891756 1102 22-23 0.15445149667966973 1102 24-25 0.2589073657280281 1102 26-27 0.3256265278398658 1102 28-29 0.3783620414759099 1102 30-31 0.3375344094955608 1102 32-33 0.4922229664868283 1102 34-35 0.49680454696795806 1102 36-37 0.7821803881903762 1102 38-39 0.9284630558471392 1102 40-41 1.208293126097523 1102 42-43 1.1993839004640279 1102 44-45 1.3997116754444292 1102 46-47 1.5673948465850387 1102 48-49 1.9257309362971924 1102 50-51 1.7587473716204336 1102 52-53 1.874430895466876 1102 54-55 1.818232696032581 1102 56-57 1.958848438211053 1102 58-59 1.862045597112786 1102 60-61 2.1080853494647336 1102 62-63 2.0200198564068295 1102 64-65 2.1830651870214197 1102 66-67 2.222495091815958 1102 68-69 2.3407159513837037 1102 70-71 2.39970670759255 1102 72-73 2.5713185724926433 1102 74-75 2.2487628216710362 1102 76-77 2.1861318617345056 1102 78-79 2.413413446505462 1102 80-81 2.490725885868539 1102 82-83 2.2902368092697216 1102 84-85 2.2927826256106165 1102 86-87 2.3027823401217837 1102 88-89 2.63320424301903 1102 90-91 2.394923982789674 1102 92-93 2.6623360831436003 1102 94-95 2.398043831492835 1102 96-97 2.435657372331999 1102 98-99 2.5424404987953544 1102 100-101 2.5367022328952054 1103 1 -0.04546061476200336 1103 2 -0.044687185011561326 1103 3 -0.15142132569149425 1103 4 -0.08470351399987663 1103 5 -0.10307000068431194 1103 6 -0.1410945679401081 1103 7 -0.14928195551456014 1103 8 -0.08858036622098098 1103 9 0.09494280221514373 1103 10-11 -0.10269291254338242 1103 12-13 -0.01821718321374277 1103 14-15 -0.04514572827843466 1103 16-17 -0.45925222050131254 1103 18-19 -0.045797073791142395 1103 20-21 0.0410832993184016 1103 22-23 -0.032649416760961 1103 24-25 -0.03182556163169892 1103 26-27 -0.17829909199484462 1103 28-29 0.12793576918360827 1103 30-31 0.24527038165737025 1103 32-33 0.37278408001444774 1103 34-35 0.5205409541014987 1103 36-37 0.6571347161590637 1103 38-39 0.3384500066953322 1103 40-41 0.7296350138748195 1103 42-43 1.1913021257793872 1103 44-45 1.354657303978577 1103 46-47 1.5109724890382736 1103 48-49 1.3922272390375028 1103 50-51 1.331424622599119 1103 52-53 1.4749876592772289 1103 54-55 1.7665972023396677 1103 56-57 1.8478610523911279 1103 58-59 1.887948163445973 1103 60-61 1.8036442881337216 1103 62-63 1.9755831447930845 1103 64-65 1.9249268660122851 1103 66-67 1.9611292805936849 1103 68-69 2.078986938769525 1103 70-71 1.9311268902806766 1103 72-73 1.925350760400427 1103 74-75 1.865657123541414 1103 76-77 1.8044680948793506 1103 78-79 2.413535238588981 1103 80-81 2.210932497438783 1103 82-83 2.3663851346285725 1103 84-85 2.1470931954235795 1103 86-87 2.1541242278990786 1103 88-89 1.8743090712052002 1103 90-91 1.8559766143686218 1103 92-93 1.8356592671366414 1103 94-95 1.7560734095702593 1103 96-97 2.052608220526867 1103 98-99 2.11853880240562 1103 100-101 2.3421198057529686 1104 1 -0.09761553035286852 1104 2 0.11616463006070887 1104 3 -0.011030726552135661 1104 4 -0.09444421796104052 1104 5 -0.3073025947480872 1104 6 -0.16541322622102683 1104 7 -0.14397686889864758 1104 8 -0.09396931027924182 1104 9 0.05060428296103936 1104 10-11 0.004865291141925354 1104 12-13 -0.009222369134526787 1104 14-15 0.039444363302713725 1104 16-17 -0.057537556482770924 1104 18-19 -0.07439466840661169 1104 20-21 -0.09696591175960378 1104 22-23 -0.06197304028517436 1104 24-25 -0.21970595443761454 1104 26-27 -0.04370789702628741 1104 28-29 -0.13844843030884135 1104 30-31 -0.17662303111985267 1104 32-33 -0.38015865417952455 1104 34-35 -0.6741892676793668 1104 36-37 -0.32678230910850914 1104 38-39 -0.4843702514973174 1104 40-41 -0.5071828168655586 1104 42-43 -0.14347002472266013 1104 44-45 -0.4907625349268656 1104 46-47 -0.447420978908319 1104 48-49 -0.33432026179003316 1104 50-51 -0.5214408744769052 1104 52-53 -0.4526477815260357 1104 54-55 -0.4418856399862747 1104 56-57 -0.30088439613584583 1104 58-59 -0.4431894289627252 1104 60-61 -0.21412907609445497 1104 62-63 -0.27097759668324883 1104 64-65 -0.15523202177321593 1104 66-67 -0.4056300706145244 1104 68-69 -0.18857567846538714 1104 70-71 -0.05312997830588273 1104 72-73 -0.2562712190765666 1104 74-75 -0.3640548923473652 1104 76-77 -0.4661001496312984 1104 78-79 -0.3372492698519345 1104 80-81 -0.13862725098888973 1104 82-83 -0.1574237586738647 1104 84-85 -0.042162018081882024 1104 86-87 0.052191093175476766 1104 88-89 0.02562761914738232 1104 90-91 0.1276283853053961 1104 92-93 0.13631688602663417 1104 94-95 0.25885173904173797 1104 96-97 1.487700137907666E-4 1104 98-99 0.4181746845638443 1104 100-101 0.7968963698046032 1105 1 -0.04105286335735059 1105 2 -0.17935064103724585 1105 3 -0.026651230518922375 1105 4 -0.02986576603519353 1105 5 -0.25698812933928394 1105 6 -0.1088505592255089 1105 7 -0.19774428781590103 1105 8 -0.05031411235101757 1105 9 -0.27282236233020285 1105 10-11 0.013990953985675958 1105 12-13 -0.1115161168222798 1105 14-15 -0.022914332796268866 1105 16-17 -0.06261422027197483 1105 18-19 -0.06730378173747908 1105 20-21 -0.39944874975853395 1105 22-23 -0.3487737144332357 1105 24-25 -0.2617785486744708 1105 26-27 -0.26348427718003364 1105 28-29 -0.501645700831304 1105 30-31 -0.08055693172412504 1105 32-33 -0.1790035590177652 1105 34-35 -0.15355375980745833 1105 36-37 -0.19552896919812213 1105 38-39 0.17370513798761777 1105 40-41 0.43267824286231615 1105 42-43 0.5060963007593102 1105 44-45 0.447515921109634 1105 46-47 0.6563526059973483 1105 48-49 1.1177289016908745 1105 50-51 1.22583581397625 1105 52-53 1.4441746791144077 1105 54-55 1.6839621834198226 1105 56-57 1.420001861931322 1105 58-59 1.4611966441247937 1105 60-61 1.637681669168007 1105 62-63 1.7029813789405424 1105 64-65 1.6098643731788975 1105 66-67 1.5930545185251184 1105 68-69 1.2369802881560652 1105 70-71 1.369959592952391 1105 72-73 1.630295147512001 1105 74-75 1.988927390712611 1105 76-77 1.6671879664537137 1105 78-79 1.6923885810955746 1105 80-81 1.6094510159573048 1105 82-83 1.6580076549817448 1105 84-85 1.4623391534559147 1105 86-87 1.3959038403288453 1105 88-89 1.7568110778558115 1105 90-91 2.031089560131722 1105 92-93 1.9962050761035002 1105 94-95 1.655633093092792 1105 96-97 1.6652122796943942 1105 98-99 1.5555529062927889 1105 100-101 1.6668967808704984 1106 1 -0.03279507995724984 1106 2 -0.09627583405352169 1106 3 -0.01618969823575611 1106 4 -0.032285371870358404 1106 5 -0.14434091991788023 1106 6 -0.2291130011198277 1106 7 -0.16973308333410841 1106 8 -0.1157790079866885 1106 9 -0.2194253762956464 1106 10-11 -0.12632861470201107 1106 12-13 -0.34760840319919595 1106 14-15 0.09618097557797967 1106 16-17 0.20683800506260042 1106 18-19 3.605742399770406E-5 1106 20-21 -0.34102681467625473 1106 22-23 -0.1415410005591582 1106 24-25 -0.2007462504505142 1106 26-27 0.14289906468718527 1106 28-29 0.2012949736463696 1106 30-31 0.4151660886668296 1106 32-33 0.09202492406004836 1106 34-35 0.14650748332778818 1106 36-37 0.42379976588014046 1106 38-39 0.24213179180326705 1106 40-41 0.06655473252629207 1106 42-43 -0.39379125282842864 1106 44-45 -0.03143049616694782 1106 46-47 0.5769875266322586 1106 48-49 0.30304460503314345 1106 50-51 0.8216210814882778 1106 52-53 0.44246037332040444 1106 54-55 0.6966651140544364 1106 56-57 1.128984021103232 1106 58-59 1.0279918209768937 1106 60-61 1.3642360902195811 1106 62-63 1.216929251996575 1106 64-65 1.0432072443775553 1106 66-67 1.1791161833344113 1106 68-69 1.5337359079667117 1106 70-71 1.6294121194827156 1106 72-73 1.5808588855520185 1106 74-75 1.355839029920265 1106 76-77 1.3488265717166037 1106 78-79 1.6878355053849994 1106 80-81 1.8271076126461594 1106 82-83 1.8457981833794825 1106 84-85 1.7793100278271687 1106 86-87 2.0124264350298127 1106 88-89 2.0870546448166465 1106 90-91 2.0838640749555744 1106 92-93 2.0298285613398903 1106 94-95 1.9198570622503475 1106 96-97 1.9477113008082156 1106 98-99 1.9699455450687289 1106 100-101 1.671537705340647 1107 1 -0.06366206243713535 1107 2 -0.1582192669646929 1107 3 -0.15194641463763503 1107 4 -0.0733625742378834 1107 5 -0.22979310214425652 1107 6 -0.11825074509628308 1107 7 -0.35488126848229484 1107 8 -0.023027673299871765 1107 9 -0.24669380678988517 1107 10-11 -0.1423690259037116 1107 12-13 0.0373023849373979 1107 14-15 0.15756314179885322 1107 16-17 -0.27792722970262673 1107 18-19 -0.24685952011674317 1107 20-21 -0.2595276686609864 1107 22-23 -0.18600728696092972 1107 24-25 -0.31682372136670267 1107 26-27 -0.535175548871301 1107 28-29 -0.1785885836565413 1107 30-31 -0.2615067009091874 1107 32-33 -0.5793494005400817 1107 34-35 -0.653386171404577 1107 36-37 -0.6061434092243232 1107 38-39 -0.33366875173921784 1107 40-41 -0.5392964055829168 1107 42-43 -0.6192217363234249 1107 44-45 -0.5169020138965337 1107 46-47 -0.336265886621149 1107 48-49 -0.48200905098825686 1107 50-51 -0.24133209436812564 1107 52-53 -0.2730579792947232 1107 54-55 0.15066541482893214 1107 56-57 0.16228136891670886 1107 58-59 0.1418188541587675 1107 60-61 -0.004067649051897604 1107 62-63 0.08518196702348746 1107 64-65 0.2848876070256594 1107 66-67 0.5164132948250675 1107 68-69 0.08231904525952771 1107 70-71 0.19868424520645078 1107 72-73 0.6224946691232844 1107 74-75 0.6702504249768211 1107 76-77 0.7028462079943054 1107 78-79 0.5775372015383091 1107 80-81 0.7156411221474102 1107 82-83 0.8563790004119127 1107 84-85 0.767883282529457 1107 86-87 0.5219760252245607 1107 88-89 0.8623841540171249 1107 90-91 1.0114740993397895 1107 92-93 0.9311960110944355 1107 94-95 0.8446956645083041 1107 96-97 0.7240129561421291 1107 98-99 0.7040743195200818 1107 100-101 1.0102930360314595 1108 1 -0.1445007906076512 1108 2 -0.2232926313420016 1108 3 -0.08009637759038668 1108 4 -0.11435792845829695 1108 5 -0.05848336006885546 1108 6 0.014821905377758071 1108 7 -0.058032718733514344 1108 8 -0.010734475391821263 1108 9 -0.10624415589939673 1108 10-11 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14 4070.0 15 6125.0 16 8697.0 17 12182.0 18 9656.0 19 4402.0 20 2966.0 21 2658.0 22 2754.0 23 2807.0 24 3073.0 25 3354.0 26 3584.0 27 4016.0 28 4719.0 29 5575.0 30 6837.0 31 8001.0 32 9939.0 33 12451.0 34 17128.0 35 24237.0 36 34225.0 37 58906.0 38 57634.0 39 5973.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.691922029649476 22.35997999493699 16.281281064960883 17.666816910452646 2 15.845714726529353 23.717657949548716 40.49741572166937 19.939211602252566 3 18.377536064182674 27.975314356244695 35.064414101674 18.582735477898634 4 10.838540461312968 20.96829437630178 39.57232122193937 28.620843940445884 5 9.350541935165296 41.812026269659036 34.903278769473864 13.9341530257018 6 23.937051608423975 42.10938825889069 18.74874643215305 15.204813700532286 7 22.625626783923476 41.49132145336728 19.619532515621387 16.263519247087864 8 26.358713260819254 34.19270230656484 20.104913985960042 19.343670446655867 9 27.638972460078687 15.912365964668673 22.391113168248094 34.05754840700455 10-11 23.225642212450822 29.26575638355319 27.84694900871712 19.66165239527887 12-13 25.240466954887562 29.91263882588557 28.215097961161156 16.631796258065712 14-15 21.78770346370439 28.900408855974696 31.316053382704624 17.99583429761629 16-17 16.171102368278948 32.60063256962123 31.40924168788089 19.81902337421893 18-19 16.14795957725835 28.920620226799354 38.80583198333719 16.12558821260511 20-21 17.50181285196328 26.75352927563064 39.70870940368742 16.03594846871866 22-23 17.549985111310313 27.321902728255953 39.21634577554644 15.911766384887285 24-25 17.129112486654282 27.67469775421663 39.81868338712763 15.377506372001456 26-27 16.641003486900978 27.952602832721325 40.528589502268034 14.877804178109669 28-29 15.841785803552968 28.943860374552177 40.07905772571721 15.135296096177642 30-31 16.89575635081656 27.66387151023305 40.28527567133898 15.155096467611415 32-33 15.837229036488468 28.12419964514387 40.3979017202808 15.640669598086863 34-35 16.291444881586052 28.750134999614286 39.141093882588905 15.817326236210755 36-37 17.029289606694768 28.110554996698284 38.702580274875494 16.15757512173146 38-39 15.670803581897964 29.738732511710285 38.50139165746095 16.0890722489308 40-41 16.39700130679828 29.51096121416526 38.099727068930136 15.992310410106317 42-43 16.231037840223312 29.07646894534053 37.952557374468995 16.739935839967163 44-45 17.423435933040192 29.481601481138625 36.69088945460156 16.404073131219626 46-47 17.700377998920004 29.906194553729847 35.54516701380853 16.84826043354162 48-49 17.63221111195111 30.00637199162538 35.26226222674108 17.099154669682434 50-51 17.450281570624085 30.420427370207513 33.77104065416956 18.358250404998845 52-53 18.089849849108536 31.620555798984185 32.677567469158284 17.612026882749003 54-55 18.504849965054333 30.281308773137038 32.32991179497307 18.883929466835557 56-57 18.737637892463162 31.941525881354625 31.652703849417573 17.668132376764635 58-59 19.14911671680938 31.32021908508833 31.420350227570776 18.110313970531514 60-61 18.438170176656637 30.403764560672684 33.021059939828746 18.137005322841933 62-63 18.34544472730078 30.141325310499113 32.83992902877421 18.673300933425903 64-65 17.96698295147728 30.151508138548177 33.041425595926874 18.840083314047675 66-67 18.084548329861917 31.133379618915374 31.669520944225876 19.112551106996836 68-69 18.294376301781995 29.952171565224102 32.03687418035949 19.71657795263442 70-71 18.06958265833526 30.631952480135773 32.80074056931266 18.497724292216308 72-73 18.285119185373755 29.97809149116717 33.357093265447816 18.379696058011262 74-75 17.59484687186608 31.368973231505056 32.18143948160148 18.854740415027386 76-77 17.410321684795186 30.806449124431072 32.53691275167785 19.24631643909589 78-79 17.168710946540152 31.22579649772429 31.581269767800663 20.024222787934892 80-81 16.89871171796652 30.03779989200031 32.39404458844403 20.66944380158914 82-83 16.339915667539408 30.72981675509798 32.332071792134215 20.598195785228395 84-85 16.561752680706626 30.487387178893776 31.856051839851883 21.09480830054771 86-87 16.117411093111162 30.461004397130292 31.43253876417496 21.989045745583585 88-89 16.561598395433155 30.116948237290753 31.61660109542544 21.70485227185065 90-91 16.212959319863295 28.91799939824565 32.84205491394142 22.02698636794964 92-93 15.88967164400174 28.49300611906724 33.04882602161886 22.56849621531217 94-95 15.756229267916378 28.54863843246162 32.98434004474273 22.710792254879273 96-97 16.606495410013114 28.37769034945614 31.92686878037491 23.088945460155827 98-99 16.45385256271793 28.542598821242326 31.42098929243682 23.582559323602926 100-101 15.983236611585932 27.525347989982702 32.516560481052466 23.9748549173789 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1115.0 1 725.5 2 384.0 3 444.5 4 388.0 5 393.5 6 661.0 7 1098.0 8 1457.5 9 1599.0 10 1425.0 11 1127.5 12 1023.5 13 996.0 14 978.5 15 1077.0 16 1256.5 17 1428.5 18 1630.0 19 1896.5 20 2167.0 21 2429.0 22 2721.5 23 3084.5 24 3604.5 25 4457.0 26 6132.5 27 8751.5 28 9537.0 29 9505.0 30 9945.0 31 10243.5 32 10659.0 33 10948.0 34 12682.5 35 14501.5 36 15465.0 37 15873.5 38 13401.5 39 10382.0 40 9591.5 41 9040.0 42 8387.0 43 8220.5 44 8075.0 45 7618.0 46 7219.5 47 7230.0 48 8175.5 49 8009.5 50 6785.5 51 5923.5 52 5068.0 53 4316.0 54 3696.5 55 3209.5 56 2700.5 57 2255.5 58 1986.0 59 1812.5 60 1663.0 61 1553.0 62 1278.0 63 871.0 64 564.5 65 419.5 66 315.0 67 233.5 68 163.0 69 136.0 70 121.0 71 99.0 72 75.0 73 52.0 74 37.0 75 32.5 76 25.0 77 19.0 78 14.5 79 11.0 80 11.0 81 10.0 82 6.5 83 3.0 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04844557586978323 2 0.0 3 0.0 4 0.0 5 0.015737097894006017 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0055542698449433 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010799969142945307 24-25 3.0857054694129443E-4 26-27 0.0015428527347064722 28-29 0.0024685643755303554 30-31 7.714263673532361E-4 32-33 0.0 34-35 0.0 36-37 3.0857054694129443E-4 38-39 3.0857054694129443E-4 40-41 1.5428527347064721E-4 42-43 0.01280567769806372 44-45 0.0 46-47 0.0 48-49 1.5428527347064721E-4 50-51 0.0 52-53 3.0857054694129443E-4 54-55 1.5428527347064721E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5428527347064721E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.006942837306179126 92-93 0.0018514232816477668 94-95 0.0 96-97 0.0 98-99 0.0015428527347064722 100-101 0.010954254416415954 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 324075.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.35002610839986 #Duplication Level Percentage of deduplicated Percentage of total 1 77.29942985486758 33.50932302372933 2 12.40687870356043 10.756770314461898 3 4.1833058727479235 5.440392564091348 4 1.9301578030373516 3.346895646200036 5 1.0300550779854012 2.2326457261878496 6 0.6134923353456377 1.5956945252721961 7 0.4175305906628987 1.266997340440459 8 0.2891577507693054 1.0027996836236455 9 0.1986778079032823 0.7751419343790246 >10 1.2938021991702904 11.189156695497129 >50 0.1780126603111138 5.349322826868384 >100 0.14027397632515767 12.560259385349188 >500 0.009968708977422372 2.830850527709749 >1k 0.007832557053689009 4.7171581904329285 >5k 0.0014241012824889104 3.426591615756834 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6034 1.8619146802437707 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5067 1.5635269613515392 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3482 1.0744426444495871 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1832 0.5653012419964515 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1376 0.42459307259122114 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 1219 0.37614749672143794 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1103 0.3403533132762478 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1084 0.3344904728843632 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1057 0.3261590681169482 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1039 0.32060479827200494 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1037 0.31998765717812233 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.31967908663118105 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1017 0.31381624623929644 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 887 0.2737020751369282 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 783 0.24161073825503354 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 779 0.24037645606726837 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 757 0.23358790403455992 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 749 0.23111933965902953 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 673 0.20766797809149118 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 643 0.19841086168325234 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 583 0.17989662886677468 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 578 0.1783537761320682 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 566 0.17465092956877265 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 562 0.17341664738100748 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 555 0.17125665355241843 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 551 0.17002237136465323 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 505 0.1558281262053537 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 497 0.15335956182982333 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 490 0.15119956800123427 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 486 0.1499652858134691 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 483 0.1490395741726452 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 463 0.14286816323381935 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 458 0.14132531049911287 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 450 0.1388567461235825 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 448 0.13823960502969992 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 444 0.13700532284193476 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 439 0.13546247010722828 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 439 0.13546247010722828 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 439 0.13546247010722828 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 421 0.12990820026228497 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 413 0.1274396358867546 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 410 0.12651392424593072 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 406 0.12527964205816555 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 384 0.11849109002545707 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 379 0.1169482372907506 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 373 0.11509681400910282 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 361 0.11139396744580729 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 356 0.10985111471110082 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 345 0.10645683869474659 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 340 0.10491398596004012 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 339 0.10460541541309881 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 332 0.10244542158450975 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 328 0.10121113939674459 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.5428527347064721E-4 0.0 16-17 0.0 0.0 0.0 3.0857054694129443E-4 0.0 18-19 0.0 0.0 0.0 3.0857054694129443E-4 0.0 20-21 0.0 0.0 0.0 4.6285582041194164E-4 0.0 22-23 0.0 0.0 0.0 0.0010799969142945304 0.0 24-25 0.0 0.0 0.0 0.0024685643755303554 0.0 26-27 0.0 0.0 0.0 0.0058628403918845945 0.0 28-29 0.0 0.0 0.0 0.01280567769806372 0.0 30-31 0.0 0.0 0.0 0.02561135539612744 0.0 32-33 0.0 0.0 0.0 0.0359484687186608 0.0 34-35 0.0 0.0 0.0 0.05276556352696135 0.0 36-37 0.0 0.0 0.0 0.06773123505361413 0.0 38-39 0.0 0.0 0.0 0.09719972228650775 0.0 40-41 0.0 0.0 0.0 0.1564452672992363 0.0 42-43 0.0 0.0 0.0 0.20890226027925635 0.0 44-45 0.0 0.0 0.0 0.2647535292756307 0.0 46-47 0.0 0.0 0.0 0.36750752140708165 0.0 48-49 0.0 0.0 0.0 0.4361644681015197 0.0 50-51 0.0 0.0 0.0 0.5427755920697369 0.0 52-53 0.0 0.0 0.0 0.6621923937360179 0.0 54-55 0.0 0.0 0.0 0.8070662655249556 0.0 56-57 0.0 0.0 0.0 1.1514309959114404 0.0 58-59 0.0 0.0 0.0 1.455064414101674 0.0 60-61 0.0 0.0 0.0 1.723212219393659 0.0 62-63 0.0 0.0 0.0 2.044125588212605 0.0 64-65 0.0 0.0 0.0 2.3454447273007792 0.0 66-67 0.0 0.0 0.0 2.800431998765718 0.0 68-69 0.0 0.0 0.0 3.3634189616601096 0.0 70-71 0.0 0.0 0.0 3.7195093728303634 0.0 72-73 0.0 0.0 0.0 4.097354007559979 0.0 74-75 0.0 0.0 0.0 4.693820874797501 0.0 76-77 0.0 0.0 0.0 5.417418807374837 0.0 78-79 0.0 0.0 0.0 6.109851114711101 0.0 80-81 0.0 0.0 0.0 6.7942605878268925 0.0 82-83 0.0 0.0 0.0 7.550566998380004 0.0 84-85 0.0 0.0 0.0 8.360101828280492 0.0 86-87 0.0 0.0 0.0 9.212219393658875 0.0 88-89 0.0 0.0 0.0 10.406850266142097 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGT 15 6.6149683E-4 94.995056 6 GGTATCA 3095 0.0 40.079193 1 GGCATTA 50 0.0016096248 38.021496 1 GCTTCGA 25 0.0016144835 38.00389 20-21 GTATCAA 5085 0.0 36.544746 1 CGCCGAA 55 0.0025756112 34.54899 7 CATTCCG 255 0.0 33.532845 9 CATGGGT 400 0.0 32.060837 4 TACGCCG 60 0.003947807 31.66502 5 TTAGGCA 245 0.0 31.018797 4 GGCATTC 300 0.0 30.086412 7 TAGGACA 95 3.48443E-5 29.99844 4 AGGCATT 270 0.0 29.905851 6 GCCTATG 65 0.0058218497 29.247303 1 GAGTACC 65 3.0307092E-7 29.233763 12-13 GCCGAAG 65 0.005835042 29.233763 8 ACGCCGA 65 0.0058394447 29.229252 6 TTACGCC 65 0.0058394447 29.229252 4 TAAGACT 65 0.0058394447 29.229252 4 GCATTCC 310 0.0 29.115885 8 >>END_MODULE