##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937489_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 356400 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.677645903479238 33.0 31.0 34.0 30.0 34.0 2 31.718599887766555 33.0 31.0 34.0 30.0 34.0 3 31.6929657687991 33.0 31.0 34.0 30.0 34.0 4 35.38544051627385 37.0 35.0 37.0 33.0 37.0 5 35.42975869809203 37.0 35.0 37.0 33.0 37.0 6 35.53736531986532 37.0 35.0 37.0 33.0 37.0 7 35.49414983164983 37.0 35.0 37.0 33.0 37.0 8 35.4998569023569 37.0 35.0 37.0 33.0 37.0 9 37.05168911335578 39.0 37.0 39.0 33.0 39.0 10-11 36.97487794612795 39.0 37.0 39.0 33.0 39.0 12-13 36.87898428731762 39.0 37.0 39.0 33.0 39.0 14-15 37.93066919191919 40.0 38.0 41.0 33.0 41.0 16-17 37.92772727272727 40.0 38.0 41.0 33.0 41.0 18-19 38.08103956228956 40.0 38.0 41.0 33.5 41.0 20-21 38.12614758698092 40.0 38.0 41.0 34.0 41.0 22-23 37.99575476992143 40.0 38.0 41.0 33.0 41.0 24-25 37.82967312008979 40.0 37.5 41.0 33.0 41.0 26-27 37.63380331088665 40.0 37.0 41.0 33.0 41.0 28-29 37.42101711560045 39.5 36.5 41.0 33.0 41.0 30-31 37.14902076318743 39.0 36.0 41.0 32.5 41.0 32-33 36.72502805836139 39.0 35.0 41.0 32.0 41.0 34-35 36.41473484848485 39.0 35.0 41.0 31.0 41.0 36-37 36.15794612794613 39.0 35.0 41.0 30.5 41.0 38-39 35.92842312008979 39.0 35.0 41.0 30.0 41.0 40-41 35.59040404040404 39.0 35.0 41.0 29.5 41.0 42-43 35.050927328844 38.5 35.0 41.0 26.0 41.0 44-45 34.36697109988776 38.0 35.0 41.0 19.5 41.0 46-47 33.41803170594837 38.0 34.0 40.5 2.0 41.0 48-49 32.66984427609428 38.0 33.0 40.0 2.0 41.0 50-51 31.595958193041525 37.5 32.0 39.5 2.0 40.5 52-53 31.622585578002244 38.0 32.5 39.5 2.0 40.5 54-55 31.858078002244667 38.0 33.0 40.0 2.0 41.0 56-57 31.61709736251403 38.0 32.0 40.0 2.0 41.0 58-59 31.35884399551066 38.0 31.5 40.0 2.0 41.0 60-61 31.087550505050505 38.0 31.0 40.0 2.0 41.0 62-63 30.756272446689113 37.0 31.0 40.0 2.0 41.0 64-65 30.39303731762065 37.0 30.0 40.0 2.0 41.0 66-67 29.982010381593717 36.0 29.0 39.0 2.0 41.0 68-69 29.45906144781145 35.5 28.5 39.0 2.0 40.5 70-71 29.041896745230076 35.0 28.0 38.5 2.0 40.0 72-73 28.639751683501686 35.0 27.0 37.5 2.0 40.0 74-75 28.197742704826037 35.0 26.0 37.0 2.0 39.0 76-77 27.74512766554433 35.0 26.0 36.5 2.0 39.0 78-79 27.277523849607185 34.0 24.5 36.0 2.0 39.0 80-81 26.818100448933784 34.0 23.0 36.0 2.0 37.5 82-83 26.377897025813695 34.0 20.5 35.0 2.0 37.0 84-85 25.95692901234568 34.0 18.5 35.0 2.0 36.5 86-87 25.545764590347922 34.0 11.5 35.0 2.0 36.0 88-89 25.134890572390574 33.5 4.5 35.0 2.0 36.0 90-91 24.718187429854098 33.0 2.0 35.0 2.0 35.0 92-93 24.314875140291804 33.0 2.0 35.0 2.0 35.0 94-95 23.834913019079686 32.5 2.0 35.0 2.0 35.0 96-97 23.302285353535353 32.0 2.0 35.0 2.0 35.0 98-99 22.736762065095398 32.0 2.0 35.0 2.0 35.0 100-101 21.311666666666667 29.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.028701897076157223 1101 2 0.030528837379598883 1101 3 0.07705153781796881 1101 4 0.019893317484779516 1101 5 0.0017867385414618298 1101 6 -0.05260817203144086 1101 7 -0.12442398972469704 1101 8 -0.021566571991165517 1101 9 0.1391224022071853 1101 10-11 0.03783661843946362 1101 12-13 0.119691683940637 1101 14-15 0.013721082959143871 1101 16-17 -0.2580641927885452 1101 18-19 0.17321992517165796 1101 20-21 0.29949986374928983 1101 22-23 0.16874784769344586 1101 24-25 0.19787967374286808 1101 26-27 0.3252403500400547 1101 28-29 0.1326790387247172 1101 30-31 -0.03970657017683976 1101 32-33 0.1729879489808539 1101 34-35 0.08370339497283652 1101 36-37 0.2161701683430408 1101 38-39 0.44439376535415676 1101 40-41 0.6159934207867863 1101 42-43 0.6551044919106488 1101 44-45 0.4261222356216834 1101 46-47 0.23248419123164155 1101 48-49 0.42837858962336384 1101 50-51 0.47285615289258587 1101 52-53 0.38222325924114386 1101 54-55 0.5926629095388734 1101 56-57 0.626215869706563 1101 58-59 0.5411191821996866 1101 60-61 0.8025925334457824 1101 62-63 0.6509947105619638 1101 64-65 0.9761820648840782 1101 66-67 1.0458060356222099 1101 68-69 0.9742866685433036 1101 70-71 0.8399527705964189 1101 72-73 1.0876108402694946 1101 74-75 0.9386852433197923 1101 76-77 0.4269370042600542 1101 78-79 0.7804350114436112 1101 80-81 1.053162483689146 1101 82-83 0.9850215564876805 1101 84-85 0.8763909973633055 1101 86-87 0.9414417623904185 1101 88-89 0.5730097846128999 1101 90-91 0.6525768300953558 1101 92-93 0.7924363031952311 1101 94-95 0.6957093470869289 1101 96-97 0.35285789772458287 1101 98-99 0.4350651933856504 1101 100-101 0.43333198719858856 1102 1 -0.09314873382073685 1102 2 0.008912377222728196 1102 3 0.06541575289129042 1102 4 -0.10684079641768562 1102 5 -0.1866002115551595 1102 6 -0.0744838114539661 1102 7 -0.02994177988043134 1102 8 -0.07712642194000807 1102 9 0.012652014575031956 1102 10-11 -0.16021044305878007 1102 12-13 0.175694866843898 1102 14-15 0.27346645069814457 1102 16-17 -0.05708886028882176 1102 18-19 -0.026907390958839983 1102 20-21 0.1034284757761128 1102 22-23 -0.018370488102853244 1102 24-25 0.10866289529567297 1102 26-27 0.0013253790272074184 1102 28-29 0.04601995039915607 1102 30-31 0.13494842584453437 1102 32-33 0.3165959971790997 1102 34-35 0.34421265949483626 1102 36-37 0.4151266307734076 1102 38-39 0.706301233809306 1102 40-41 0.8486477347574564 1102 42-43 0.7503665130542245 1102 44-45 0.49410222880118226 1102 46-47 0.5688488610054279 1102 48-49 0.6598847849172174 1102 50-51 0.49029164226396205 1102 52-53 0.567502331652193 1102 54-55 0.7858946931014543 1102 56-57 0.7196954650231504 1102 58-59 0.6318864892361766 1102 60-61 0.9167359914428275 1102 62-63 1.0568808080952152 1102 64-65 1.0159547146510448 1102 66-67 1.1915216204806853 1102 68-69 0.8686915793083365 1102 70-71 1.072247871198897 1102 72-73 0.8723920156702007 1102 74-75 1.2211770018738441 1102 76-77 1.421050906726805 1102 78-79 1.4476783898680772 1102 80-81 1.445523515859204 1102 82-83 1.0966459739027101 1102 84-85 1.0006037971814266 1102 86-87 1.3643927684151969 1102 88-89 1.580305453590963 1102 90-91 1.7932929833294118 1102 92-93 1.5858908899861852 1102 94-95 1.1803836678781074 1102 96-97 1.2438957515383855 1102 98-99 1.0609217353886073 1102 100-101 1.5406390236882999 1103 1 -0.3711006072412424 1103 2 -0.2910238935167513 1103 3 -0.13040009152141252 1103 4 -0.05651226109189622 1103 5 -0.19649802498225455 1103 6 -0.20331361037716533 1103 7 -0.3488386044052092 1103 8 -0.3392866241155943 1103 9 -0.1338725431658716 1103 10-11 -0.1947934559145068 1103 12-13 -0.104799584315316 1103 14-15 0.06174905700515154 1103 16-17 -0.20249485962956015 1103 18-19 -0.11330888530012118 1103 20-21 -0.12223683572811694 1103 22-23 -0.2867322065953246 1103 24-25 -0.10623436874124792 1103 26-27 -0.3991427014035125 1103 28-29 -0.4926878839047717 1103 30-31 -0.2725914049432916 1103 32-33 0.07128669050950265 1103 34-35 0.2611338769434681 1103 36-37 0.35362130168869754 1103 38-39 0.4086416359629155 1103 40-41 0.8401014211655493 1103 42-43 1.0107049374031973 1103 44-45 1.3688236879814895 1103 46-47 1.6347756822338582 1103 48-49 1.8005858067829408 1103 50-51 1.6448307039055408 1103 52-53 1.5050696319488601 1103 54-55 1.4076527416675475 1103 56-57 1.6478659957340582 1103 58-59 1.3479792262980403 1103 60-61 1.6535824918273931 1103 62-63 1.685917177724921 1103 64-65 1.7810310798856293 1103 66-67 1.7151585834517888 1103 68-69 1.6509528692764732 1103 70-71 1.5444487282475734 1103 72-73 1.507293674828535 1103 74-75 1.7725296004893814 1103 76-77 1.4901356226920832 1103 78-79 1.6605758736610667 1103 80-81 1.4543357342967376 1103 82-83 1.329897264969624 1103 84-85 1.31341557610682 1103 86-87 1.3183428837871354 1103 88-89 1.3763234735887622 1103 90-91 1.6441291551786605 1103 92-93 1.5381313031275958 1103 94-95 1.5721054566925226 1103 96-97 1.3973755889188908 1103 98-99 1.7093361953380644 1103 100-101 1.7766746241717613 1104 1 -0.15984433881260074 1104 2 0.01370890462370511 1104 3 -0.274768728931825 1104 4 -0.10113271177308292 1104 5 -0.008976771511754578 1104 6 0.06928178811195806 1104 7 -0.10701177445449161 1104 8 0.06320998609037787 1104 9 0.0075733385034126854 1104 10-11 -0.05436128407885832 1104 12-13 0.03931939207449631 1104 14-15 -0.03690793888405608 1104 16-17 0.10502725474101737 1104 18-19 0.17677139363150474 1104 20-21 0.0065538148971171495 1104 22-23 -0.19025328788581675 1104 24-25 -0.345129833950125 1104 26-27 0.023365530953412872 1104 28-29 -0.08460403223784851 1104 30-31 -0.11098754811097677 1104 32-33 0.13359002864952885 1104 34-35 0.1828995829891369 1104 36-37 0.27048202957971057 1104 38-39 0.4455850102607499 1104 40-41 0.3785771975897987 1104 42-43 0.44316087008080984 1104 44-45 0.498182532653594 1104 46-47 0.7037918886776922 1104 48-49 0.5990383389052809 1104 50-51 0.17031334600785897 1104 52-53 0.39685664527129205 1104 54-55 0.5483797717775225 1104 56-57 0.14234332177843712 1104 58-59 -0.07734845379149746 1104 60-61 -0.11239043286536798 1104 62-63 0.10022318465516733 1104 64-65 0.21735460613232505 1104 66-67 0.11380051358974796 1104 68-69 0.021800640621414402 1104 70-71 0.18299122442732596 1104 72-73 0.12248425658175677 1104 74-75 -0.013885396389856197 1104 76-77 -0.024353325503255974 1104 78-79 0.3996263008827263 1104 80-81 0.43016811705291147 1104 82-83 0.46895199669164356 1104 84-85 0.30543288019825 1104 86-87 0.6924448574005488 1104 88-89 0.5766592246164635 1104 90-91 0.6461307478437952 1104 92-93 0.37260819872193807 1104 94-95 0.36458334232195355 1104 96-97 0.3718176180603585 1104 98-99 0.5768522834081402 1104 100-101 0.7646650681809746 1105 1 0.27433664629622 1105 2 0.281543956170097 1105 3 0.5886906066924595 1105 4 0.5500013088454736 1105 5 0.3081366305501021 1105 6 0.23592554527117926 1105 7 0.18105237216635572 1105 8 0.2580782564225643 1105 9 0.4352251139902421 1105 10-11 0.3979182119119855 1105 12-13 0.427039896083663 1105 14-15 0.48030855596130806 1105 16-17 0.3077649408807659 1105 18-19 0.23040255056162806 1105 20-21 0.1616740898111999 1105 22-23 -0.028019610909872483 1105 24-25 0.19749330465239723 1105 26-27 0.31027274744825917 1105 28-29 0.5518679036155234 1105 30-31 0.5103870223357561 1105 32-33 0.9008065234948859 1105 34-35 0.5205284489685198 1105 36-37 0.9176412506564517 1105 38-39 1.3193421694818213 1105 40-41 1.3035600154592117 1105 42-43 1.2186706066214654 1105 44-45 1.5097747434210689 1105 46-47 1.6913634808884623 1105 48-49 1.9554988200049337 1105 50-51 2.0835664960651314 1105 52-53 2.1011865421785068 1105 54-55 1.8930876755575952 1105 56-57 1.8725609620991719 1105 58-59 2.151769530171851 1105 60-61 2.3329933013843487 1105 62-63 2.1012082934753273 1105 64-65 2.3460716737153717 1105 66-67 2.388714602936826 1105 68-69 2.5678143863258818 1105 70-71 2.4355227250000624 1105 72-73 2.0207545887696163 1105 74-75 1.982872908306593 1105 76-77 1.9833900880133513 1105 78-79 2.101596518523049 1105 80-81 1.9896755626428266 1105 82-83 1.852376968054756 1105 84-85 1.8314809901638824 1105 86-87 1.6638079730935615 1105 88-89 1.9479077927722486 1105 90-91 2.063702340054558 1105 92-93 2.007235919225952 1105 94-95 1.8040678784044246 1105 96-97 2.210328500076397 1105 98-99 2.0557755365581976 1105 100-101 2.4003466260274777 1106 1 -0.2516950997355245 1106 2 -0.06797985335371237 1106 3 -0.07638875838690495 1106 4 0.039048927893091445 1106 5 -0.19805384564037354 1106 6 0.0362430055886378 1106 7 -0.07259842148444307 1106 8 0.13157031991462986 1106 9 -0.021759012993889826 1106 10-11 -0.06819289919912563 1106 12-13 -0.13256327851950545 1106 14-15 -0.06778668213393502 1106 16-17 -0.23612394800812098 1106 18-19 -0.12467681451773416 1106 20-21 -0.14023067209355844 1106 22-23 0.009599436709173403 1106 24-25 -0.3002844731253802 1106 26-27 0.08932036649587616 1106 28-29 -0.021386064638448943 1106 30-31 0.1415774985262388 1106 32-33 0.2693779520663213 1106 34-35 -0.13899536055528472 1106 36-37 0.024863472878678294 1106 38-39 -0.29081656067690886 1106 40-41 -0.2509637940645959 1106 42-43 -0.16815479020392843 1106 44-45 0.23961601326233506 1106 46-47 -0.029271439746452188 1106 48-49 0.3826416771477952 1106 50-51 0.27967760717623946 1106 52-53 0.20071035170231966 1106 54-55 0.4676114850813988 1106 56-57 0.48716413670234715 1106 58-59 0.37684889525121434 1106 60-61 0.43624726963831506 1106 62-63 0.7387479760150129 1106 64-65 0.8243256419693381 1106 66-67 0.7649050791272991 1106 68-69 0.5278143863258826 1106 70-71 0.5373481218254597 1106 72-73 0.9780561760712025 1106 74-75 0.9396983051319907 1106 76-77 0.8487075483308146 1106 78-79 0.9683425502690781 1106 80-81 0.6103104832777504 1106 82-83 0.5332499839277745 1106 84-85 0.2919571806400718 1106 86-87 0.43206194134752707 1106 88-89 0.6867966816611357 1106 90-91 0.7844959908482103 1106 92-93 0.771442268432299 1106 94-95 0.9343059736425197 1106 96-97 0.9778681826160778 1106 98-99 0.9823628381455016 1106 100-101 1.3380450387258946 1107 1 -0.09257805884172043 1107 2 -0.3099366816431157 1107 3 -0.22123093215846268 1107 4 -0.2753289872820943 1107 5 -0.21468797081664093 1107 6 -0.025770227950793867 1107 7 -0.4131668128501005 1107 8 -0.147348217887739 1107 9 -0.24778678173235846 1107 10-11 -0.12128705257801897 1107 12-13 0.08278274599508251 1107 14-15 -0.07228825497263358 1107 16-17 -0.18614293054418596 1107 18-19 -0.2091924886689469 1107 20-21 -0.29556205293746984 1107 22-23 -0.3693787604922534 1107 24-25 -0.18650315194087597 1107 26-27 -0.1719823778364784 1107 28-29 -0.08525687492155498 1107 30-31 -0.21606703992696197 1107 32-33 -0.4771889206964559 1107 34-35 -0.3160319154961684 1107 36-37 -0.29296112848806644 1107 38-39 -0.1690734215077967 1107 40-41 0.11708317415321545 1107 42-43 0.08221401335394063 1107 44-45 0.22206530277313163 1107 46-47 0.03647864160726755 1107 48-49 0.20757930595785723 1107 50-51 0.15494336268117692 1107 52-53 0.19084485652930638 1107 54-55 0.39204743258112984 1107 56-57 0.557182576880237 1107 58-59 0.39822865444418554 1107 60-61 0.4918037291241575 1107 62-63 0.5334735427793014 1107 64-65 0.8525282669828975 1107 66-67 0.49015727567790535 1107 68-69 0.7472145381861708 1107 70-71 0.8087902522083681 1107 72-73 0.8961709390606813 1107 74-75 0.8649559252643257 1107 76-77 0.9396745729538765 1107 78-79 1.174783053577464 1107 80-81 1.116390318400864 1107 82-83 1.245743204450605 1107 84-85 1.2180717266862828 1107 86-87 1.199292660514466 1107 88-89 1.296401844910946 1107 90-91 1.3264257012289455 1107 92-93 1.2553477896385026 1107 94-95 0.8142956688372287 1107 96-97 0.9838693758040584 1107 98-99 1.2116854327870037 1107 100-101 1.4824397670044505 1108 1 0.009669009226836778 1108 2 -0.2043075722141836 1108 3 -0.1837499561411171 1108 4 0.006245850330188318 1108 5 0.180538377274992 1108 6 0.05709973255890333 1108 7 0.03653029550454079 1108 8 -0.07998093814317997 1108 9 0.30258076658606825 1108 10-11 0.08439079803522276 1108 12-13 0.12742320515692995 1108 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-2.535108410485144 2316 78-79 -3.1855506286240995 2316 80-81 -3.1897538667865994 2316 82-83 -2.905971380293593 2316 84-85 -3.155726217043327 2316 86-87 -3.752107942822356 2316 88-89 -3.837077192212739 2316 90-91 -4.134615978263756 2316 92-93 -3.893454771464743 2316 94-95 -3.783229418892873 2316 96-97 -3.7140558843079887 2316 98-99 -3.8286088478261853 2316 100-101 -3.8400437643629104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1723.0 3 299.0 4 240.0 5 203.0 6 288.0 7 344.0 8 472.0 9 581.0 10 827.0 11 1414.0 12 2365.0 13 3517.0 14 4497.0 15 6061.0 16 8735.0 17 12016.0 18 8675.0 19 4137.0 20 3713.0 21 3290.0 22 3270.0 23 3453.0 24 3833.0 25 4050.0 26 4176.0 27 4680.0 28 5308.0 29 6407.0 30 7380.0 31 8974.0 32 10896.0 33 14012.0 34 18998.0 35 28060.0 36 38193.0 37 62219.0 38 60262.0 39 8832.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.30878974867129 21.781258445185117 15.555467975858031 18.35448383028556 2 15.220095343693188 22.888114682591052 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16.873188859190435 46-47 17.488840305652605 31.09305251910329 34.197099533928984 17.221007641315122 48-49 17.190112159861197 30.933596919083765 34.34099578609623 17.535295134958808 50-51 17.069034612220793 31.108111338437634 33.32419340096883 18.49866064837274 52-53 17.897972556817063 32.05027969097593 32.178383501466655 17.87336425074036 54-55 18.074535733671578 30.742966748321663 32.162132517345825 19.020365000660927 56-57 18.32300581414964 32.282306766680534 31.584777499128442 17.809909920041385 58-59 18.859908025815734 31.744305021475633 31.103409116463155 18.292377836245475 60-61 18.13365532209184 30.47713561199418 33.02839985830995 18.36080920760403 62-63 18.17954358567081 30.367507010675904 32.845385604745466 18.60756379890781 64-65 17.65306997384116 30.55088182417493 33.06064043473433 18.735407767249583 66-67 17.947304938018213 31.37172483404514 31.69421568930663 18.98675453863002 68-69 18.013384479794663 30.00445587071509 32.1368078816903 19.845351767799936 70-71 17.687468810840826 31.03888892793635 32.80656747049769 18.467074790725135 72-73 17.809139784946236 30.422177981733928 33.37691771269177 18.391764520628065 74-75 17.398614453766392 31.695354146517012 32.569614701185365 18.33641669853123 76-77 17.391065953022654 31.10003247670745 32.32782072857486 19.18108084169503 78-79 17.218375452532424 31.495834944290184 31.52142279708973 19.76436680608766 80-81 17.166633866569203 30.04034411987967 32.51707947932188 20.275942534229245 82-83 16.72461327143614 30.836497785000994 32.46565732257162 19.973231620991243 84-85 17.230270130468885 30.505008194716588 31.8265643756132 20.438157299201333 86-87 16.98177923096603 30.83122243057918 31.46097899030906 20.726019348145734 88-89 17.388804381749832 30.342436673536582 31.55076379770397 20.717995147009617 90-91 17.315464734916166 29.01435457818402 32.87972997479409 20.790450712105727 92-93 17.515527775668982 28.563374975045903 33.32630400980731 20.594793239477806 94-95 17.33666045759674 28.80047798123221 33.117070256212 20.745791304959056 96-97 18.267124770502914 28.76311964213918 31.873033635205765 21.09672195215214 98-99 18.454067975796836 28.329059540679758 31.802359583417683 21.41451290010572 100-101 18.846318192296234 27.56281590680363 32.94876336015203 20.642102540748112 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1435.0 1 903.0 2 441.5 3 478.5 4 390.5 5 443.5 6 779.5 7 1373.0 8 1900.5 9 1990.0 10 1619.5 11 1250.0 12 1163.0 13 1162.5 14 1178.0 15 1191.5 16 1316.5 17 1520.0 18 1741.0 19 2025.5 20 2279.5 21 2540.0 22 2841.5 23 3091.0 24 3488.5 25 4432.0 26 6400.0 27 9415.5 28 10259.5 29 10487.5 30 11378.5 31 11772.0 32 12180.0 33 12255.5 34 13991.0 35 15863.0 36 17115.5 37 17796.0 38 14956.0 39 11567.0 40 10385.0 41 9598.0 42 9020.5 43 8787.0 44 8739.5 45 8283.0 46 7721.0 47 7908.5 48 9016.0 49 8721.0 50 7385.5 51 6377.5 52 5527.5 53 4884.0 54 4086.5 55 3530.0 56 3048.5 57 2626.0 58 2296.0 59 2007.0 60 1811.0 61 1703.0 62 1439.5 63 996.5 64 649.5 65 513.0 66 363.5 67 240.5 68 201.5 69 189.0 70 155.5 71 120.5 72 104.5 73 80.0 74 52.0 75 35.5 76 26.5 77 17.5 78 10.5 79 7.5 80 8.0 81 9.5 82 7.5 83 3.5 84 3.0 85 1.5 86 0.5 87 1.5 88 2.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3277216610549944 2 0.2957351290684624 3 0.3005050505050505 4 0.297979797979798 5 0.28507295173961844 6 0.2208193041526375 7 0.2281144781144781 8 0.22839506172839508 9 0.2222222222222222 10-11 0.23484848484848483 12-13 0.23330527497194162 14-15 0.21534792368125702 16-17 0.22797418630751964 18-19 0.21787317620650953 20-21 0.21801346801346802 22-23 0.21801346801346802 24-25 0.2080527497194164 26-27 0.22292368125701462 28-29 0.2227833894500561 30-31 0.22923681257014591 32-33 0.22923681257014591 34-35 0.21885521885521886 36-37 0.22011784511784513 38-39 0.20552749719416386 40-41 0.20454545454545456 42-43 0.20791245791245788 44-45 0.22124017957351289 46-47 0.21534792368125702 48-49 0.22208193041526372 50-51 0.22937710437710437 52-53 0.2326038159371493 54-55 0.23540965207631873 56-57 0.20089786756453423 58-59 0.18181818181818182 60-61 0.1947250280583614 62-63 0.19430415263748596 64-65 0.19318181818181818 66-67 0.20510662177328845 68-69 0.19346240179573512 70-71 0.1985129068462402 72-73 0.21548821548821548 74-75 0.2042648709315376 76-77 0.21338383838383837 78-79 0.21394500561167226 80-81 0.19921436588103253 82-83 0.2122615039281706 84-85 0.191638608305275 86-87 0.1983726150392817 88-89 0.20847362514029183 90-91 0.20468574635241302 92-93 0.2095959595959596 94-95 0.2069304152637486 96-97 0.20510662177328845 98-99 0.20875420875420878 100-101 0.19023569023569023 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 356400.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.552912021814976 #Duplication Level Percentage of deduplicated Percentage of total 1 77.42464778747782 33.720688734080326 2 12.208713535622854 10.634500530330477 3 4.424528177688193 5.781032593826856 4 1.9221994113245882 3.3486952739921727 5 0.9780205806434507 2.1297822152144303 6 0.6234269420602906 1.629123525574859 7 0.3926690488905379 1.1971316378013555 8 0.26376370018576734 0.9190141782991286 9 0.1856161781478711 0.7275712569029789 >10 1.2233898343265863 10.482869094231402 >50 0.1881081070448336 5.647321119225179 >100 0.1468789328980208 13.350101028168346 >500 0.010951499382747162 3.5733446827529747 >1k 0.0064420584604395065 5.121812749972954 >5k 6.442058460439507E-4 1.7370113796265443 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6191 1.7370931537598204 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4641 1.3021885521885521 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3492 0.9797979797979799 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1926 0.5404040404040404 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1326 0.372053872053872 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1291 0.3622334455667789 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1172 0.3288439955106622 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1126 0.3159371492704826 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 1117 0.3134118967452301 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1114 0.3125701459034792 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1050 0.2946127946127946 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 995 0.2791806958473625 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 982 0.27553310886644217 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 898 0.2519640852974186 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 896 0.2514029180695847 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 888 0.24915824915824916 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 847 0.23765432098765432 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 833 0.23372615039281708 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 828 0.23232323232323235 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 768 0.21548821548821548 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 704 0.19753086419753088 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 694 0.1947250280583614 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 616 0.17283950617283952 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 609 0.17087542087542087 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 570 0.15993265993265993 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 540 0.15151515151515152 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 536 0.1503928170594837 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 532 0.14927048260381595 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 493 0.13832772166105498 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 490 0.13748597081930414 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 486 0.13636363636363638 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 482 0.13524130190796857 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 481 0.13496071829405162 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 479 0.13439955106621773 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 479 0.13439955106621773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 466 0.13075196408529743 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 464 0.13019079685746351 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 456 0.12794612794612795 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 451 0.12654320987654322 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 449 0.1259820426487093 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 444 0.12457912457912458 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 442 0.12401795735129068 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 435 0.12205387205387204 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 427 0.11980920314253647 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 419 0.11756453423120089 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 411 0.11531986531986532 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 410 0.11503928170594838 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 402 0.1127946127946128 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 400 0.11223344556677892 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 400 0.11223344556677892 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 394 0.11054994388327723 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 377 0.1057800224466891 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 363 0.10185185185185185 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 362 0.1015712682379349 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.4029180695847364E-4 0.0 18-19 0.0 0.0 0.0 5.611672278338945E-4 0.0 20-21 0.0 0.0 0.0 8.417508417508417E-4 0.0 22-23 0.0 0.0 0.0 0.0016835016835016834 0.0 24-25 0.0 0.0 0.0 0.0021043771043771043 0.0 26-27 0.0 0.0 0.0 0.003928170594837261 0.0 28-29 0.0 0.0 0.0 0.011083052749719416 0.0 30-31 0.0 0.0 0.0 0.02441077441077441 0.0 32-33 0.0 0.0 0.0 0.038720538720538725 0.0 34-35 0.0 0.0 0.0 0.05387205387205388 0.0 36-37 0.0 0.0 0.0 0.07435465768799102 0.0 38-39 0.0 0.0 0.0 0.10185185185185186 0.0 40-41 0.0 0.0 0.0 0.1654040404040404 0.0 42-43 0.0 0.0 0.0 0.2160493827160494 0.0 44-45 0.0 0.0 0.0 0.27441077441077444 0.0 46-47 0.0 0.0 0.0 0.39225589225589225 0.0 48-49 0.0 0.0 0.0 0.4710998877665544 0.0 50-51 0.0 0.0 0.0 0.5827721661054994 0.0 52-53 0.0 0.0 0.0 0.6992143658810326 0.0 54-55 0.0 0.0 0.0 0.8500280583613917 0.0 56-57 0.0 0.0 0.0 1.1734006734006734 0.0 58-59 0.0 0.0 0.0 1.472783389450056 0.0 60-61 0.0 0.0 0.0 1.7512626262626263 0.0 62-63 0.0 0.0 0.0 2.0531705948372614 0.0 64-65 0.0 0.0 0.0 2.343574635241302 0.0 66-67 0.0 0.0 0.0 2.790544332210999 0.0 68-69 0.0 0.0 0.0 3.3378226711560046 0.0 70-71 0.0 0.0 0.0 3.6864478114478114 0.0 72-73 0.0 0.0 0.0 4.0479797979797985 0.0 74-75 0.0 0.0 0.0 4.585437710437711 0.0 76-77 0.0 0.0 0.0 5.262626262626263 0.0 78-79 0.0 0.0 0.0 5.922138047138047 0.0 80-81 0.0 0.0 0.0 6.557098765432099 0.0 82-83 0.0 0.0 0.0 7.297138047138047 0.0 84-85 0.0 0.0 0.0 8.086560044893378 0.0 86-87 0.0 0.0 0.0 8.920314253647586 0.0 88-89 0.0 0.0 0.0 10.03956228956229 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 230 0.0 53.700344 9 TTAGGCA 225 0.0 52.82699 4 GGCATTC 230 0.0 51.634945 7 TAGGCAT 240 0.0 51.484566 5 GCATTCC 245 0.0 50.41257 8 AGGCATT 300 0.0 41.17026 6 GGTATCA 2700 0.0 40.68824 1 GTATCAA 4505 0.0 37.581654 1 CCTTTAG 330 0.0 36.02855 1 CTTTAGG 330 0.0 34.577667 2 CACAGTA 60 0.0039456226 31.669436 7 TATCAAC 5710 0.0 29.559057 2 ATCAACG 5850 0.0 28.77039 3 TTTAGGC 450 0.0 28.526575 3 TCAACGC 5920 0.0 28.510511 4 CAACGCA 6005 0.0 28.095081 5 GGCTATG 85 6.353144E-4 27.975107 1 ATGGGTA 290 0.0 27.85902 5 AACGCAG 6090 0.0 27.691252 6 ACGCAGG 70 0.0083344085 27.17582 1 >>END_MODULE