FastQCFastQC Report
Thu 26 May 2016
SRR937488_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937488_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411388
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66331.6123464952793956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51481.2513733993213219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38490.9356130951802192No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21220.515814753954904No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT15890.38625336665143367No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC14110.34298521104164437No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA13690.3327758709539413No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA13420.3262127237547036No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT13040.31697570177059126No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA12330.29971705543185506No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT11830.2875630791369705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10730.26082433128822424No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10480.25474734314078196No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT10230.24867035499333964No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC10010.24332260542359035No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG9940.24162104874230653No Hit
GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT9860.23967641253512498No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA9860.23967641253512498No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG8730.21220842610868573No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.20637451748714108No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA8410.20442988127995954No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT8150.19810981360661953No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT7910.1922759049850749No Hit
GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT7060.17161414528377106No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC7050.17137106575787334No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6610.16067556661837487No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6590.16018940756657948No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG5980.14536155648682023No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA5820.14147228407245716No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5570.13539529592501484No Hit
CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA5560.13515221639911712No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC5560.13515221639911712No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA5510.13393681876962868No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5490.1334506597178333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5470.1329645006660379No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC5460.1327214211401402No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA5390.13101986445885636No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA5280.12834598967398175No Hit
AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA5260.12785983062218637No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC5230.12713059204449328No Hit
GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG5090.12372747868192559No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.12202592200064175No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.12081052437115326No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT4870.11837972911217633No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT4830.11740741100858558No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.1161920133790971No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG4590.11157350238704095No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT4460.10841346855037094No Hit
GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT4450.10817038902447323No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT4430.10768422997267788No Hit
GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA4270.10379495755831478No Hit
GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC4180.10160724182523555No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGA200.002072626771.2542959
GGGTACG250.005017780757.0034377
GGTATCA32600.043.1366961
GTCGGGA1500.041.169152
CGGGAGT1600.038.5913734
GTATCAA53600.037.1382981
TTAGGCA2350.034.3595854
CATGGGT5250.032.569424
GGGAGTG2350.032.3423775
GTCTAGG753.071734E-431.672441
CGCTGTA300.00395149131.66471324-25
GGAGTGG2150.030.9320986
TAGGCAT2500.030.4018345
TCGGGAG2051.8189894E-1230.1200943
CATTCCG2550.029.8057179
GGCATTC2550.029.8057177
TATCAAC66800.029.7248482
ATCAACG67800.029.2828563
TCAACGC68050.028.9658874
GTACATG37100.028.812531
Produced by FastQC (version 0.11.5)