##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937488_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 411388 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.726292453839196 33.0 31.0 34.0 30.0 34.0 2 31.77147607611306 33.0 31.0 34.0 30.0 34.0 3 31.74688615127325 33.0 31.0 34.0 30.0 34.0 4 35.42948992192286 37.0 35.0 37.0 33.0 37.0 5 35.49770776007078 37.0 35.0 37.0 33.0 37.0 6 35.573042966737 37.0 35.0 37.0 33.0 37.0 7 35.47886909681371 37.0 35.0 37.0 33.0 37.0 8 35.49084076346418 37.0 35.0 37.0 33.0 37.0 9 37.05302050618881 39.0 37.0 39.0 33.0 39.0 10-11 36.963428685328694 39.0 37.0 39.0 33.0 39.0 12-13 36.84425408616683 39.0 37.0 39.0 33.0 39.0 14-15 37.90994632804068 40.0 38.0 41.0 33.0 41.0 16-17 37.894625025523354 40.0 38.0 41.0 33.0 41.0 18-19 38.05395757776114 40.0 38.0 41.0 33.5 41.0 20-21 38.05560930313962 40.0 38.0 41.0 33.0 41.0 22-23 37.927599978608995 40.0 38.0 41.0 33.0 41.0 24-25 37.813322216496346 40.0 37.0 41.0 33.0 41.0 26-27 37.73494122337064 40.0 37.0 41.0 33.0 41.0 28-29 37.609668974301634 40.0 37.0 41.0 33.0 41.0 30-31 37.217794393613815 39.0 36.5 41.0 33.0 41.0 32-33 36.756270236370526 39.0 35.0 41.0 32.0 41.0 34-35 36.5533049092341 39.0 35.0 41.0 31.0 41.0 36-37 36.276513899287295 39.0 35.0 41.0 30.5 41.0 38-39 36.003879549233325 39.0 35.0 41.0 30.0 41.0 40-41 35.62826212723755 39.0 35.0 41.0 29.5 41.0 42-43 35.07491954067693 39.0 35.0 41.0 26.0 41.0 44-45 34.39493981350938 38.0 35.0 41.0 20.0 41.0 46-47 33.472717969410866 38.0 34.0 40.0 3.5 41.0 48-49 32.7360691123708 38.0 33.0 40.0 2.0 41.0 50-51 31.62155677851566 37.5 32.0 39.5 2.0 40.5 52-53 31.649439215533754 37.5 32.5 39.5 2.0 40.5 54-55 31.917675041566596 38.0 33.0 40.0 2.0 41.0 56-57 31.690920736628193 38.0 32.5 40.0 2.0 41.0 58-59 31.431274125642947 38.0 31.5 40.0 2.0 41.0 60-61 31.144919151749686 38.0 31.0 40.0 2.0 41.0 62-63 30.833173305978782 37.0 31.0 40.0 2.0 41.0 64-65 30.418039660855445 36.5 30.0 39.5 2.0 41.0 66-67 30.049792410084883 36.0 29.5 39.0 2.0 41.0 68-69 29.535769152235844 35.5 29.0 39.0 2.0 40.0 70-71 29.07603406030317 35.0 28.0 38.5 2.0 40.0 72-73 28.638624607426564 35.0 27.0 37.5 2.0 40.0 74-75 28.176301933940707 35.0 26.0 37.0 2.0 39.0 76-77 27.693629857944323 34.5 26.0 36.5 2.0 39.0 78-79 27.207039339990473 34.0 24.5 36.0 2.0 38.5 80-81 26.722641399360214 34.0 23.0 35.5 2.0 37.5 82-83 26.256019864458857 34.0 20.5 35.0 2.0 37.0 84-85 25.779613163242487 34.0 18.0 35.0 2.0 36.5 86-87 25.343767927115035 33.0 11.0 35.0 2.0 36.0 88-89 24.88707254465371 33.0 4.5 35.0 2.0 36.0 90-91 24.42189490213618 32.5 2.0 35.0 2.0 35.0 92-93 23.988203350608185 32.0 2.0 35.0 2.0 35.0 94-95 23.531800878975567 32.0 2.0 35.0 2.0 35.0 96-97 22.917586317539644 31.5 2.0 34.5 2.0 35.0 98-99 22.177727595360096 31.0 2.0 34.0 2.0 35.0 100-101 20.137968292706642 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.03760918680452008 1101 2 0.10392871264996018 1101 3 0.21094448956581857 1101 4 0.19254743884675207 1101 5 0.0611300744175054 1101 6 0.003582881356692269 1101 7 0.16879446366011308 1101 8 0.0711926715980411 1101 9 0.25485834908923266 1101 10-11 0.19109785790106315 1101 12-13 0.08591269284119107 1101 14-15 0.0582335931884117 1101 16-17 -0.011495825999148224 1101 18-19 0.11852517969319365 1101 20-21 0.21944426786446058 1101 22-23 0.05405609838728509 1101 24-25 0.134792213076814 1101 26-27 0.11706587819954706 1101 28-29 0.15000521992170235 1101 30-31 0.2914266689314502 1101 32-33 0.17697556107412282 1101 34-35 0.4215607842315805 1101 36-37 0.4495949613324157 1101 38-39 0.3170866147724283 1101 40-41 0.3811869505146319 1101 42-43 0.7838970607943097 1101 44-45 1.1474255749398878 1101 46-47 1.1881037192270867 1101 48-49 1.3077937923479652 1101 50-51 0.9705426829827282 1101 52-53 1.010697558672792 1101 54-55 1.0664619048456636 1101 56-57 0.9416859404157272 1101 58-59 1.356674286748369 1101 60-61 1.4572048545051217 1101 62-63 1.0906532714217754 1101 64-65 1.2093779926825725 1101 66-67 1.0440233603992546 1101 68-69 1.0507122881473556 1101 70-71 0.8764651633880867 1101 72-73 0.6995612297710885 1101 74-75 0.8883637542714453 1101 76-77 0.8114152476181253 1101 78-79 0.7282739600558656 1101 80-81 0.742236162174855 1101 82-83 0.9151338343806223 1101 84-85 0.7934878804465519 1101 86-87 0.9119577788840942 1101 88-89 0.9703465513808247 1101 90-91 0.9559798453212771 1101 92-93 1.3429582062092464 1101 94-95 1.1300263594666156 1101 96-97 1.498924036807761 1101 98-99 1.2787819977557682 1101 100-101 1.4044617299455524 1102 1 0.11297935406528481 1102 2 0.08967041816134014 1102 3 0.05372241967580038 1102 4 -0.1619943458773747 1102 5 0.19319448047423293 1102 6 0.18742881256346777 1102 7 0.2681226786313218 1102 8 0.19066301251751838 1102 9 0.08755188715704776 1102 10-11 0.018935806900238106 1102 12-13 0.17588771035939743 1102 14-15 0.30366172285358317 1102 16-17 0.2729331567263813 1102 18-19 0.21409078917434954 1102 20-21 0.28967131901762144 1102 22-23 0.23949580530012327 1102 24-25 0.11712738962984304 1102 26-27 0.20746933004733847 1102 28-29 0.06848844814581412 1102 30-31 0.1621556547036178 1102 32-33 0.11570183533303435 1102 34-35 0.047463352552576055 1102 36-37 0.22169265197616284 1102 38-39 0.22500179620008254 1102 40-41 0.2197148120751251 1102 42-43 0.3839488537443785 1102 44-45 0.2658010281281946 1102 46-47 0.3901476366020944 1102 48-49 0.6753257059451343 1102 50-51 0.6755285160875708 1102 52-53 0.9120053306619447 1102 54-55 0.9757556927383 1102 56-57 0.9330220461952088 1102 58-59 0.8464394666527042 1102 60-61 1.2659574822356774 1102 62-63 1.1815575153970386 1102 64-65 1.1457716800359528 1102 66-67 1.0940835316206545 1102 68-69 1.319241596863808 1102 70-71 1.0438439831181547 1102 72-73 1.335245429874675 1102 74-75 1.3024560066554436 1102 76-77 1.4084337255147226 1102 78-79 1.3681015961646885 1102 80-81 1.4825973418354579 1102 82-83 1.3021947367966114 1102 84-85 1.0856001188159894 1102 86-87 1.1519267031140572 1102 88-89 1.2682131388347457 1102 90-91 1.3662618884008282 1102 92-93 1.453701815873199 1102 94-95 1.4970308228002516 1102 96-97 1.6245162282588765 1102 98-99 1.5146779396257948 1102 100-101 1.6333827609299227 1103 1 0.08769363977956957 1103 2 0.004527561018480952 1103 3 0.013881149834531925 1103 4 -0.02597847286150312 1103 5 0.0807500360297908 1103 6 -0.15855531442065995 1103 7 -0.039877321368678054 1103 8 -0.2735750827205763 1103 9 -0.027114779509616938 1103 10-11 -0.019921335956901487 1103 12-13 0.07130040877209609 1103 14-15 0.2727172784091394 1103 16-17 0.1794331567263754 1103 18-19 -0.03565524257168562 1103 20-21 -4.636016173051871E-4 1103 22-23 0.218725964030277 1103 24-25 0.06632580232825802 1103 26-27 0.2558105998886049 1103 28-29 0.23167892433628623 1103 30-31 0.20181438486235237 1103 32-33 0.40109072422192327 1103 34-35 0.30427287636209854 1103 36-37 0.44687519165871237 1103 38-39 0.761954177152468 1103 40-41 0.9213021136624349 1103 42-43 1.0543615521570686 1103 44-45 0.5434121392393081 1103 46-47 0.9767428746973366 1103 48-49 1.4150241186435437 1103 50-51 1.5884332779923334 1103 52-53 1.466925965582579 1103 54-55 1.635104899087505 1103 56-57 1.5591331573063236 1103 58-59 1.414812482525715 1103 60-61 1.5203622441404434 1103 62-63 1.273668626508151 1103 64-65 1.173882791147065 1103 66-67 1.3620041665412934 1103 68-69 1.7187257238479354 1103 70-71 1.7033519196260904 1103 72-73 1.4845708267000717 1103 74-75 1.4828052130046494 1103 76-77 1.338632138213132 1103 78-79 1.0694428660059607 1103 80-81 1.3140576592957771 1103 82-83 1.1112106098124848 1103 84-85 1.4762191664350368 1103 86-87 1.4588790840664387 1103 88-89 1.2964194880410957 1103 90-91 1.4722063328452712 1103 92-93 1.389265307936693 1103 94-95 1.0520705053399375 1103 96-97 1.1311908314334822 1103 98-99 1.4417493681972253 1103 100-101 1.7170970466442093 1104 1 0.16731066105616677 1104 2 0.23833026894884668 1104 3 0.17943240708791564 1104 4 0.15662114029130692 1104 5 0.23569394318646175 1104 6 0.09888762561802622 1104 7 0.12740701131991017 1104 8 0.23831660006472077 1104 9 0.04080785105131213 1104 10-11 0.1780612559193102 1104 12-13 0.38542226563863125 1104 14-15 0.33588555693912525 1104 16-17 0.32624553583663385 1104 18-19 0.19733508624843665 1104 20-21 0.3000083519610328 1104 22-23 0.1505228692527183 1104 24-25 0.36142638259904913 1104 26-27 0.38265392677448773 1104 28-29 0.2852495239494388 1104 30-31 0.3549594525606139 1104 32-33 0.40606557915422314 1104 34-35 0.3836519866135504 1104 36-37 -0.2070725839699179 1104 38-39 0.19396191409636998 1104 40-41 0.4448997925792568 1104 42-43 0.5736574902615175 1104 44-45 0.7177003403998512 1104 46-47 0.8824740158965625 1104 48-49 1.3578500374056404 1104 50-51 1.5317272818607996 1104 52-53 1.5568389249636247 1104 54-55 1.5897451311958228 1104 56-57 1.781318843960097 1104 58-59 1.8387389815586026 1104 60-61 1.5528864220901468 1104 62-63 1.7090129205120128 1104 64-65 2.0204224429845894 1104 66-67 1.780503199423304 1104 68-69 1.5249249501535438 1104 70-71 1.8592203915796688 1104 72-73 2.1311278866613854 1104 74-75 2.0885924470472013 1104 76-77 1.680295581153171 1104 78-79 1.583421589410218 1104 80-81 1.7775721660655996 1104 82-83 1.951806354493339 1104 84-85 1.956236574558826 1104 86-87 1.9661479428672095 1104 88-89 1.8202724861068589 1104 90-91 1.906330123947786 1104 92-93 1.9113117296001363 1104 94-95 1.8544979714908045 1104 96-97 1.5421250674102716 1104 98-99 1.4327319600734363 1104 100-101 2.1074432748840515 1105 1 0.2656936397795704 1105 2 0.2905275610184823 1105 3 0.2818811498345326 1105 4 0.42602152713850217 1105 5 0.4067500360297913 1105 6 0.26544468557933953 1105 7 0.3021226786313207 1105 8 0.27442491727942553 1105 9 0.4648852204903804 1105 10-11 0.289078664043096 1105 12-13 0.43730040877209575 1105 14-15 0.3947172784091393 1105 16-17 0.4844331567263822 1105 18-19 0.37934475742832063 1105 20-21 0.44253639838269265 1105 22-23 0.647725964030279 1105 24-25 0.6363258023282512 1105 26-27 0.4008105998886009 1105 28-29 0.6086789243362887 1105 30-31 0.5898143848623576 1105 32-33 0.6120907242219218 1105 34-35 0.8112728763620893 1105 36-37 0.9468751916587053 1105 38-39 1.0849541771524613 1105 40-41 0.9493021136624264 1105 42-43 1.0813615521570696 1105 44-45 1.5814121392393048 1105 46-47 1.8387428746973313 1105 48-49 1.6410241186435428 1105 50-51 1.721433277992329 1105 52-53 2.2359259655825774 1105 54-55 2.0891048990874985 1105 56-57 1.9711331573063227 1105 58-59 1.9598124825257166 1105 60-61 1.9173622441404348 1105 62-63 2.0516686265081496 1105 64-65 1.869882791147063 1105 66-67 2.056004166541289 1105 68-69 1.7577257238479334 1105 70-71 2.1113519196260917 1105 72-73 1.9635708267000673 1105 74-75 1.9948052130046499 1105 76-77 2.0026321382131336 1105 78-79 1.828442866005961 1105 80-81 1.9550576592957754 1105 82-83 2.0982106098124866 1105 84-85 1.9832191664350383 1105 86-87 2.0238790840664365 1105 88-89 1.7674194880410923 1105 90-91 2.214206332845272 1105 92-93 2.229265307936693 1105 94-95 2.3700705053399354 1105 96-97 2.3121908314334796 1105 98-99 2.266749368197228 1105 100-101 2.3930970466442076 1106 1 -0.0984477323618016 1106 2 -0.1562458277549048 1106 3 0.026084892038273466 1106 4 0.14527308639005554 1106 5 0.09126562854537923 1106 6 -0.05935780922315104 1106 7 -0.27397711346846876 1106 8 -0.3653339184794149 1106 9 -0.4865991869940274 1106 10-11 -0.7806261176616758 1106 12-13 -0.32996154548985857 1106 14-15 -0.2017234700316024 1106 16-17 -0.11830904701582767 1106 18-19 -0.32441199932844 1106 20-21 -0.20671723987094026 1106 22-23 -0.3092844309801137 1106 24-25 -0.5383914533890035 1106 26-27 -0.6800438699658642 1106 28-29 -0.30485330019593704 1106 30-31 -0.47060141555345325 1106 32-33 -0.2816618745306769 1106 34-35 0.17002547511469146 1106 36-37 0.0244988922824092 1106 38-39 -0.08011650891822342 1106 40-41 -0.37682678446647344 1106 42-43 -0.10786713807161874 1106 44-45 0.1574474822746481 1106 46-47 -0.04808248912802782 1106 48-49 0.23355426417369074 1106 50-51 0.26227735283640286 1106 52-53 0.6646494583060694 1106 54-55 0.5318969988795992 1106 56-57 0.26788575605892007 1106 58-59 0.542276099989337 1106 60-61 0.5157780445562352 1106 62-63 0.5146020984416211 1106 64-65 0.8748661591304305 1106 66-67 0.7542120667491901 1106 68-69 0.2683411084633214 1106 70-71 0.4686575329317044 1106 72-73 0.5523670844963284 1106 74-75 0.6839611381605728 1106 76-77 0.7586321382131338 1106 78-79 1.1141642797273725 1106 80-81 0.8995898838279999 1106 82-83 1.0764580110598878 1106 84-85 0.7163792495951178 1106 86-87 0.7314237826111345 1106 88-89 0.7720452676668756 1106 90-91 0.9337572683962065 1106 92-93 0.7304586551300396 1106 94-95 0.7870289252983547 1106 96-97 1.023558814801465 1106 98-99 0.987558100005959 1106 100-101 1.4385876911348525 1107 1 0.18534022612494994 1107 2 0.053812701580731925 1107 3 0.2196241217220809 1107 4 0.36714602513046657 1107 5 0.26782634125067517 1107 6 0.29488243658335733 1107 7 0.08237970674377237 1107 8 0.2455253188858464 1107 9 0.2903631321369673 1107 10-11 0.11252444717563037 1107 12-13 -0.08453493259336398 1107 14-15 0.28065302138102766 1107 16-17 0.4559150844372226 1107 18-19 0.18636082168534784 1107 20-21 0.1534239485835016 1107 22-23 0.2152440363194401 1107 24-25 0.3940326296374863 1107 26-27 0.07997124245888187 1107 28-29 0.2469881612840794 1107 30-31 0.5908585615691777 1107 32-33 0.7257252623745316 1107 34-35 0.6977789004584807 1107 36-37 -0.040582639666588705 1107 38-39 0.1461148197227473 1107 40-41 0.2130691819355235 1107 42-43 0.1867792228398102 1107 44-45 0.08348844446018688 1107 46-47 0.0065059269061720215 1107 48-49 0.013726929888527195 1107 50-51 0.15932082819313464 1107 52-53 0.17895407803237617 1107 54-55 0.4722655416577872 1107 56-57 0.3187315508806208 1107 58-59 0.1818405949755224 1107 60-61 0.27560320799585725 1107 62-63 0.37198589558445505 1107 64-65 0.39041692769324854 1107 66-67 0.7536025601155885 1107 68-69 0.6718783342897012 1107 70-71 0.6927013172164536 1107 72-73 0.6379443206759738 1107 74-75 0.4827007953339688 1107 76-77 0.5759012145183569 1107 78-79 0.5558645527529471 1107 80-81 0.9361018360025994 1107 82-83 1.1711945455554549 1107 84-85 1.1171549094069242 1107 86-87 1.1915136222190483 1107 88-89 1.3347889659527397 1107 90-91 1.3285276179858343 1107 92-93 0.9676026573342824 1107 94-95 1.3781146820467605 1107 96-97 1.632560309345127 1107 98-99 1.683460211570722 1107 100-101 1.7378721470458167 1108 1 -0.12345503639150834 1108 2 -0.19720767319740418 1108 3 -0.33322679313899073 1108 4 -0.271412281415472 1108 5 -0.0878080087767259 1108 6 -0.38329258529642374 1108 7 -0.20489157798782287 1108 8 -0.28110257762892843 1108 9 -0.16872781413283633 1108 10-11 -0.16694577587543336 1108 12-13 -0.08242056882465221 1108 14-15 -0.158985369248704 1108 16-17 -0.2088641956157744 1108 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2316 52-53 -3.6868526603716205 2316 54-55 -3.5106431925155483 2316 56-57 -3.914836308342533 2316 58-59 -3.5141188151842044 2316 60-61 -3.4602102749435346 2316 62-63 -4.663438243720858 2316 64-65 -4.828995071448354 2316 66-67 -4.63836224567245 2316 68-69 -4.295663589129166 2316 70-71 -4.81640380556475 2316 72-73 -5.228329936658707 2316 74-75 -5.214477229743444 2316 76-77 -4.9970930526265604 2316 78-79 -4.570549500406255 2316 80-81 -4.275324020093539 2316 82-83 -4.446926794767666 2316 84-85 -4.191208314480992 2316 86-87 -4.459487328147304 2316 88-89 -4.568649214248982 2316 90-91 -4.4979768732615995 2316 92-93 -4.4220782035136885 2316 94-95 -4.174914227484489 2316 96-97 -4.177366420474915 2316 98-99 -3.709899486764609 2316 100-101 -3.4921624953405264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1989.0 3 414.0 4 266.0 5 273.0 6 316.0 7 428.0 8 586.0 9 683.0 10 979.0 11 1605.0 12 2747.0 13 4171.0 14 4874.0 15 7121.0 16 9718.0 17 13493.0 18 9925.0 19 4705.0 20 4002.0 21 3658.0 22 3713.0 23 3992.0 24 4440.0 25 4793.0 26 4990.0 27 5596.0 28 6523.0 29 7830.0 30 9083.0 31 10870.0 32 13369.0 33 17105.0 34 23079.0 35 33425.0 36 45723.0 37 72875.0 38 64276.0 39 7753.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11172536172536 21.895596895596896 15.473265473265474 18.519412269412268 2 15.235941695622298 22.918727674111473 41.31550348685909 20.529827143407132 3 18.231123455887726 27.87637355943766 34.173427868333214 19.719075116341397 4 10.457001954124372 20.176455968850966 40.146778618663106 29.21976345836156 5 9.585526315789474 41.77071150097466 34.831384015594544 13.812378167641326 6 24.136720720896392 41.52637185052661 19.51485252286925 14.822054905707747 7 23.46823119916346 40.582214736472636 19.775460012041115 16.17409405232279 8 26.27660196730261 33.797293740312156 20.415882702750128 19.510221589635105 9 27.786767788035053 15.961221644911925 22.559537155279138 33.69247341177388 10-11 23.099328257075726 29.018026889216042 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36-37 17.254782029147258 29.379734358113335 36.56444453650352 16.80103907623588 38-39 15.815888263827205 30.828090978763743 36.72450619517764 16.63151456223141 40-41 16.738935761932318 30.486186835612685 36.254028205050105 16.520849197404893 42-43 16.39308107897347 30.09506449325337 36.24245466707932 17.26939976069384 44-45 17.537206116768466 30.533389229846787 34.98240234765356 16.94700230573118 46-47 17.655776404933953 30.92372023073201 34.02870622130627 17.39179714302777 48-49 17.40461550649303 30.748877202176732 34.19862400136503 17.647883289965204 50-51 17.336092697070857 31.036748045958518 33.04910911796646 18.57805013900417 52-53 18.164031309770955 31.95049730653563 32.02156721958165 17.863904164111766 54-55 18.293027756855118 30.628703615679566 31.957922294456186 19.120346333009135 56-57 18.53671790473034 32.36721803631827 31.181586463014042 17.914477595937345 58-59 18.991536036409173 31.693328720465054 30.838526870769844 18.476608372355923 60-61 18.312947444972718 30.56567642533722 32.7175540344812 18.40382209520886 62-63 18.38785285819401 30.287451819293725 32.53470716363654 18.789988158875726 64-65 17.82092104846431 30.630720618222657 32.63709384676478 18.911264486548248 66-67 18.201839869026273 31.430040149684256 31.23842773056833 19.129692250721135 68-69 18.245884604467637 30.018358324775456 31.821542952023496 19.91421411873341 70-71 17.877456348834123 31.007342093818487 32.585520713670526 18.52968084367687 72-73 18.08471629870834 30.421827280689897 32.938513621340974 18.554942799260793 74-75 17.56050968605746 31.81992619566555 32.13656116634727 18.48300295192972 76-77 17.588701393805067 31.20251784141157 31.89229233961616 19.316488425167204 78-79 17.456308361112736 31.60406553989512 31.102574224613527 19.83705187437862 80-81 17.45122858998147 30.129906836951875 32.162754517134715 20.25611005593194 82-83 17.12232810059718 31.051290235339117 31.82645475831386 19.999926905749838 84-85 17.50300599737592 30.59112466331002 31.392845787157114 20.513023552156948 86-87 17.238434285101633 30.858272781765816 31.08451877951432 20.81877415361823 88-89 17.694185757518166 30.464345063181337 31.148911078298074 20.692558101002422 90-91 17.50732294555433 29.315785882433214 32.41530483436252 20.76158633764993 92-93 17.709291460218875 28.771400620592107 32.9508338521178 20.568474067071215 94-95 17.708566833974952 28.88601919984406 32.860118902587594 20.54529506359339 96-97 18.69616178452286 28.7042519853889 31.711150587392446 20.88843564269579 98-99 18.671834637917083 28.277096513203425 31.557117373546355 21.49395147533313 100-101 19.078983431982934 27.47067615684373 32.78130972726907 20.669030683904257 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1624.0 1 1031.5 2 503.0 3 578.5 4 484.5 5 488.0 6 880.5 7 1521.0 8 2014.0 9 2108.0 10 1766.0 11 1444.5 12 1378.0 13 1299.5 14 1236.5 15 1243.5 16 1388.0 17 1586.5 18 1820.0 19 2143.0 20 2481.0 21 2800.0 22 3096.0 23 3415.0 24 3939.0 25 5000.0 26 7316.0 27 10737.5 28 11644.5 29 11999.0 30 12938.0 31 13440.5 32 14035.0 33 14187.5 34 16229.0 35 18478.0 36 19885.5 37 20573.5 38 17310.0 39 13294.0 40 11930.0 41 11167.0 42 10411.5 43 10015.5 44 10140.5 45 9769.5 46 9161.0 47 9192.0 48 10310.5 49 10262.0 50 8731.5 51 7579.5 52 6580.5 53 5729.0 54 4953.0 55 4322.5 56 3746.0 57 3162.5 58 2738.0 59 2408.0 60 2209.5 61 2043.0 62 1739.0 63 1231.0 64 771.5 65 578.5 66 434.0 67 322.5 68 243.0 69 204.0 70 189.5 71 160.5 72 120.0 73 85.5 74 61.5 75 40.5 76 27.0 77 21.0 78 19.5 79 18.5 80 11.0 81 5.0 82 5.0 83 3.5 84 2.5 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2751660233161881 2 0.24064873063871575 3 0.2328701858099896 4 0.23675945822435265 5 0.2401625715869204 6 0.27249214853131354 7 0.27395062568669964 8 0.2625258879695081 9 0.2566919793479635 10-11 0.27030443279823424 12-13 0.27151983042772276 14-15 0.2532888659853958 16-17 0.2709121316129785 18-19 0.25973047342168465 20-21 0.2500072923857769 22-23 0.2520734683559073 24-25 0.24563186091961847 26-27 0.2529242466965492 28-29 0.26763055801335967 30-31 0.26811671706515505 32-33 0.2650782229914339 34-35 0.27625988118272776 36-37 0.24879189475628843 38-39 0.2500072923857769 40-41 0.2432010656606415 42-43 0.25158730930411194 44-45 0.26884595564284813 46-47 0.2743152449755462 48-49 0.27723219928631854 50-51 0.2791768354935001 52-53 0.29862319756531547 54-55 0.2788122162046535 56-57 0.24696879831205576 58-59 0.2289809133956265 60-61 0.22667165789959842 62-63 0.23189786770639884 64-65 0.22387624335177495 66-67 0.22460548192946805 68-69 0.2303178507880638 70-71 0.23007477126216613 72-73 0.2303178507880638 74-75 0.23785331609089228 76-77 0.25195192859295845 78-79 0.24599648020846498 80-81 0.23785331609089228 82-83 0.23311326533588728 84-85 0.23299172557293843 86-87 0.24162104874230653 88-89 0.2387040944315342 90-91 0.25134422977821425 92-93 0.23663791846140386 94-95 0.23530098106896652 96-97 0.2467257187861581 98-99 0.2528027069336004 100-101 0.22569933979600765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 411388.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.5122007220036 #Duplication Level Percentage of deduplicated Percentage of total 1 76.47812031673121 32.51253201746419 2 12.454397491184526 10.589276920337094 3 4.630803623384445 5.9059695942450325 4 2.066136332253 3.513440099030554 5 1.1292752924743081 2.4003988952033555 6 0.675577653202525 1.723217567774753 7 0.46041561364506856 1.370129668797653 8 0.3119492137544816 1.0609318072161382 9 0.21955366413002872 0.840033849487243 >10 1.224296626764288 10.23552805474493 >50 0.1773109549387564 5.249038450195228 >100 0.14814044299721907 12.596525125024948 >500 0.0154432122043433 4.385343421933151 >1k 0.007435620690980108 4.752997386666332 >5k 0.0011439416447661702 2.864637141879371 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6633 1.6123464952793956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5148 1.2513733993213219 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3849 0.9356130951802192 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2122 0.515814753954904 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1589 0.38625336665143367 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1411 0.34298521104164437 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1369 0.3327758709539413 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 1342 0.3262127237547036 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1304 0.31697570177059126 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1233 0.29971705543185506 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1183 0.2875630791369705 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1073 0.26082433128822424 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1048 0.25474734314078196 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 1023 0.24867035499333964 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1001 0.24332260542359035 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 994 0.24162104874230653 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 986 0.23967641253512498 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 986 0.23967641253512498 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 873 0.21220842610868573 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 849 0.20637451748714108 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 841 0.20442988127995954 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 815 0.19810981360661953 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 791 0.1922759049850749 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 706 0.17161414528377106 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 705 0.17137106575787334 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 661 0.16067556661837487 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 659 0.16018940756657948 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 598 0.14536155648682023 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 582 0.14147228407245716 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 557 0.13539529592501484 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 556 0.13515221639911712 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 556 0.13515221639911712 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 551 0.13393681876962868 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 549 0.1334506597178333 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 547 0.1329645006660379 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 546 0.1327214211401402 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 539 0.13101986445885636 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 528 0.12834598967398175 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 526 0.12785983062218637 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 523 0.12713059204449328 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 509 0.12372747868192559 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 502 0.12202592200064175 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 497 0.12081052437115326 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 487 0.11837972911217633 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 483 0.11740741100858558 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 478 0.1161920133790971 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 459 0.11157350238704095 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 446 0.10841346855037094 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 445 0.10817038902447323 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 443 0.10768422997267788 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 427 0.10379495755831478 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 418 0.10160724182523555 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.4307952589769268E-4 0.0 16-17 0.0 0.0 0.0 2.4307952589769268E-4 0.0 18-19 0.0 0.0 0.0 4.8615905179538537E-4 0.0 20-21 0.0 0.0 0.0 7.29238577693078E-4 0.0 22-23 0.0 0.0 0.0 0.00267387478487462 0.0 24-25 0.0 0.0 0.0 0.004740050755005007 0.0 26-27 0.0 0.0 0.0 0.007292385776930781 0.0 28-29 0.0 0.0 0.0 0.013247834161424252 0.0 30-31 0.0 0.0 0.0 0.0267387478487462 0.0 32-33 0.0 0.0 0.0 0.04229583750619853 0.0 34-35 0.0 0.0 0.0 0.0579744669265997 0.0 36-37 0.0 0.0 0.0 0.07948700496854551 0.0 38-39 0.0 0.0 0.0 0.11315351930537594 0.0 40-41 0.0 0.0 0.0 0.17562495746108298 0.0 42-43 0.0 0.0 0.0 0.23481482201717113 0.0 44-45 0.0 0.0 0.0 0.3042140266609624 0.0 46-47 0.0 0.0 0.0 0.4135998133149241 0.0 48-49 0.0 0.0 0.0 0.5024453800305309 0.0 50-51 0.0 0.0 0.0 0.6328575456746429 0.0 52-53 0.0 0.0 0.0 0.7579219617490058 0.0 54-55 0.0 0.0 0.0 0.9200560055227669 1.2153976294884634E-4 56-57 0.0 0.0 0.0 1.2218392369247524 2.4307952589769268E-4 58-59 0.0 0.0 0.0 1.5226501502231469 2.4307952589769268E-4 60-61 0.0 0.0 0.0 1.8215164273143603 2.4307952589769268E-4 62-63 0.0 0.0 0.0 2.127310470893658 2.4307952589769268E-4 64-65 0.0 0.0 0.0 2.423989032251791 2.4307952589769268E-4 66-67 0.0 0.0 0.0 2.829324141686194 2.4307952589769268E-4 68-69 0.0 0.0 0.0 3.3416142425155813 2.4307952589769268E-4 70-71 0.0 0.0 0.0 3.707448928991609 2.4307952589769268E-4 72-73 0.0 0.0 0.0 4.075349791437767 2.4307952589769268E-4 74-75 0.0 0.0 0.0 4.597849232354857 2.4307952589769268E-4 76-77 0.0 0.0 0.0 5.227668283955779 2.4307952589769268E-4 78-79 0.0 0.0 0.0 5.846913376180151 2.4307952589769268E-4 80-81 0.0 0.0 0.0 6.469804661292988 2.4307952589769268E-4 82-83 0.0 0.0 0.0 7.15443814598384 2.4307952589769268E-4 84-85 0.0 0.0 0.0 7.914183204177078 2.4307952589769268E-4 86-87 0.0 0.0 0.0 8.712334827462152 2.4307952589769268E-4 88-89 0.0 0.0 0.0 9.73557809172849 2.4307952589769268E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTCGA 20 0.0020726267 71.254295 9 GGGTACG 25 0.0050177807 57.003437 7 GGTATCA 3260 0.0 43.136696 1 GTCGGGA 150 0.0 41.16915 2 CGGGAGT 160 0.0 38.591373 4 GTATCAA 5360 0.0 37.138298 1 TTAGGCA 235 0.0 34.359585 4 CATGGGT 525 0.0 32.56942 4 GGGAGTG 235 0.0 32.342377 5 GTCTAGG 75 3.071734E-4 31.67244 1 CGCTGTA 30 0.003951491 31.664713 24-25 GGAGTGG 215 0.0 30.932098 6 TAGGCAT 250 0.0 30.401834 5 TCGGGAG 205 1.8189894E-12 30.120094 3 CATTCCG 255 0.0 29.805717 9 GGCATTC 255 0.0 29.805717 7 TATCAAC 6680 0.0 29.724848 2 ATCAACG 6780 0.0 29.282856 3 TCAACGC 6805 0.0 28.965887 4 GTACATG 3710 0.0 28.81253 1 >>END_MODULE