##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937488_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 411388 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.992957986134744 31.0 31.0 34.0 30.0 34.0 2 32.18214921193618 33.0 31.0 34.0 30.0 34.0 3 32.16318414732564 34.0 31.0 34.0 30.0 34.0 4 35.916494890468364 37.0 35.0 37.0 35.0 37.0 5 35.864670335546975 37.0 35.0 37.0 35.0 37.0 6 35.93268398689315 37.0 35.0 37.0 35.0 37.0 7 35.86867142454325 37.0 35.0 37.0 35.0 37.0 8 35.853418184293176 37.0 35.0 37.0 35.0 37.0 9 37.46428675605511 39.0 37.0 39.0 34.0 39.0 10-11 37.41267489571888 39.0 37.0 39.0 34.0 39.0 12-13 37.31458623003102 39.0 37.0 39.0 34.0 39.0 14-15 38.325114004297646 40.0 38.0 41.0 33.5 41.0 16-17 38.34768758447014 40.0 38.0 41.0 33.5 41.0 18-19 38.54676242379457 40.0 38.0 41.0 34.0 41.0 20-21 38.571953970460974 40.0 38.0 41.0 34.0 41.0 22-23 38.418524604509614 40.0 38.0 41.0 34.0 41.0 24-25 38.297079642575866 40.0 38.0 41.0 34.0 41.0 26-27 38.12692154365222 40.0 37.0 41.0 33.5 41.0 28-29 37.937138419205226 40.0 37.0 41.0 33.0 41.0 30-31 37.66364235223196 39.0 36.5 41.0 33.0 41.0 32-33 37.27791768354935 39.0 35.0 41.0 33.0 41.0 34-35 36.99257878207435 39.0 35.0 41.0 33.0 41.0 36-37 36.64859329878363 39.0 35.0 41.0 32.0 41.0 38-39 36.34914606162552 39.0 35.0 41.0 31.0 41.0 40-41 35.97459697414606 39.0 35.0 41.0 30.5 41.0 42-43 35.438963946444716 39.0 35.0 41.0 28.5 41.0 44-45 34.64785555242253 39.0 35.0 41.0 23.5 41.0 46-47 33.60198158429512 38.0 34.0 40.0 5.0 41.0 48-49 32.759940737211586 38.0 33.0 40.0 2.0 41.0 50-51 32.30807656032748 38.0 33.0 40.0 2.0 41.0 52-53 32.020105107587 38.0 33.0 40.0 2.0 41.0 54-55 31.73210448530341 38.0 32.0 40.0 2.0 41.0 56-57 31.42596283800208 38.0 31.5 40.0 2.0 41.0 58-59 31.14533846393186 37.5 31.0 40.0 2.0 41.0 60-61 30.83540963761704 37.0 31.0 40.0 2.0 41.0 62-63 30.460565208513618 36.5 30.0 40.0 2.0 41.0 64-65 30.06338541717308 36.0 29.0 39.0 2.0 41.0 66-67 29.62412126751388 35.5 28.5 39.0 2.0 40.0 68-69 29.144159528231256 35.0 28.0 38.5 2.0 40.0 70-71 28.751801219286904 35.0 27.0 38.0 2.0 40.0 72-73 28.303072768286874 35.0 26.0 37.0 2.0 39.5 74-75 27.844320933036453 34.0 26.0 37.0 2.0 39.0 76-77 26.625508036209126 32.5 24.5 35.5 2.0 37.5 78-79 27.08902301476951 34.0 25.0 36.0 2.0 38.0 80-81 26.84649284860035 34.0 24.5 35.5 2.0 37.0 82-83 26.490173510165587 34.0 23.5 35.0 2.0 37.0 84-85 26.133891849057335 34.0 21.0 35.0 2.0 36.0 86-87 25.71046433051037 33.5 19.0 35.0 2.0 36.0 88-89 25.230543914747148 33.0 13.0 35.0 2.0 35.5 90-91 24.771466352932023 33.0 4.5 35.0 2.0 35.0 92-93 24.468583186675353 32.5 2.0 35.0 2.0 35.0 94-95 24.071650121053604 32.0 2.0 35.0 2.0 35.0 96-97 23.626676033331066 32.0 2.0 35.0 2.0 35.0 98-99 22.967554960280808 32.0 2.0 34.0 2.0 35.0 100-101 21.039663286240724 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04927157822032413 1101 2 -0.022676271047401997 1101 3 0.10837024423284447 1101 4 0.07126736805548717 1101 5 0.03485599649282989 1101 6 -0.15488622066575175 1101 7 -0.15613713052592715 1101 8 -0.06110255566952816 1101 9 -0.057625380429634276 1101 10-11 0.17851352531032205 1101 12-13 -0.10089293913161868 1101 14-15 -0.23321780190715202 1101 16-17 -0.09447467547841626 1101 18-19 -0.06453150164099952 1101 20-21 0.18452399818811216 1101 22-23 -0.002290791085350463 1101 24-25 -0.05980657998649974 1101 26-27 0.059479690759502546 1101 28-29 -0.14678602887311598 1101 30-31 -0.19715109438649847 1101 32-33 -0.125968233766784 1101 34-35 0.1441413873363544 1101 36-37 0.03223964469025731 1101 38-39 0.2277647278939341 1101 40-41 0.04065265427355058 1101 42-43 -0.1689803606027951 1101 44-45 -0.10966246152403869 1101 46-47 0.07384695845854594 1101 48-49 0.24692860507857972 1101 50-51 0.3785592424369497 1101 52-53 0.30937313069280137 1101 54-55 0.21699199202410213 1101 56-57 0.24429847506343094 1101 58-59 0.3723498029648411 1101 60-61 0.2264089853353788 1101 62-63 0.021443781672044082 1101 64-65 -0.18873777415641158 1101 66-67 0.16788567472602267 1101 68-69 0.015604046133191218 1101 70-71 0.04782272694841794 1101 72-73 0.004041418367787486 1101 74-75 -0.0960437285271567 1101 76-77 -0.27518294195480664 1101 78-79 0.12320161434969279 1101 80-81 0.23293387819064293 1101 82-83 0.4694459667438551 1101 84-85 0.444299193987586 1101 86-87 0.6865453970081532 1101 88-89 0.5087282204422934 1101 90-91 0.4576943825460873 1101 92-93 0.3261065465827748 1101 94-95 0.48891430695293536 1101 96-97 0.06823928244817168 1101 98-99 0.4207509092012103 1101 100-101 0.6956278139496916 1102 1 -0.21364619285483855 1102 2 -0.12854334596438122 1102 3 0.036473251270592755 1102 4 -0.1093529286876489 1102 5 -0.2369922501634818 1102 6 0.015226962163360724 1102 7 -0.1928491921900033 1102 8 -0.035312713181028244 1102 9 -0.2254040722006465 1102 10-11 -0.2247250573908275 1102 12-13 -0.04370362066590161 1102 14-15 0.03830864296381975 1102 16-17 -0.17827643034542717 1102 18-19 -0.2218487791598065 1102 20-21 -0.15467983753684678 1102 22-23 -0.3307076024676192 1102 24-25 -0.32175894570566044 1102 26-27 -0.5976281299759592 1102 28-29 -0.5513898889712152 1102 30-31 -0.2905669156094177 1102 32-33 -0.3456750704361937 1102 34-35 -0.4000996936129866 1102 36-37 -0.744542261290313 1102 38-39 -0.08730016562585519 1102 40-41 0.1888261911227147 1102 42-43 0.12312235700375851 1102 44-45 0.3662252544068636 1102 46-47 0.60396493975216 1102 48-49 0.9404838102700595 1102 50-51 1.0764083116871745 1102 52-53 1.1124266297635792 1102 54-55 1.1798343586572564 1102 56-57 1.0750497775037964 1102 58-59 0.6351837913266607 1102 60-61 0.6814024350505186 1102 62-63 0.8582502893038857 1102 64-65 0.6269631063237391 1102 66-67 0.6027503275609227 1102 68-69 0.6814976268540249 1102 70-71 0.8807523799356574 1102 72-73 0.8391932174111396 1102 74-75 1.0239045546888796 1102 76-77 0.866259415538412 1102 78-79 0.7524440662888807 1102 80-81 0.8641147574521355 1102 82-83 1.088558326979058 1102 84-85 1.1058132237837661 1102 86-87 0.6861664706455457 1102 88-89 1.088799740413048 1102 90-91 1.1278170861380765 1102 92-93 1.0087395325986783 1102 94-95 1.3464475153738604 1102 96-97 1.2409173587970734 1102 98-99 1.0131632420166135 1102 100-101 1.615592472877882 1103 1 0.020480791272145638 1103 2 0.12785347943243863 1103 3 0.022139917937259668 1103 4 0.08318675385203278 1103 5 0.018356956185726858 1103 6 -0.1089476410112411 1103 7 0.004738109397301571 1103 8 -0.11451906238737308 1103 9 -0.18524534204191667 1103 10-11 -0.04788378754956568 1103 12-13 -0.08121155717383743 1103 14-15 0.10058642074159962 1103 16-17 -0.24070500177399623 1103 18-19 -0.2640075093185388 1103 20-21 -0.3510211073781164 1103 22-23 -0.12558061834062784 1103 24-25 -0.008512913959634716 1103 26-27 -0.0810884474362723 1103 28-29 -0.16300100008233187 1103 30-31 -0.06135262989513279 1103 32-33 -0.2493655466266702 1103 34-35 0.10058284606955681 1103 36-37 -0.04934384859189578 1103 38-39 0.0877474534217626 1103 40-41 0.12209603239255529 1103 42-43 0.3582096585910577 1103 44-45 0.4187252544068656 1103 46-47 0.9152903365775558 1103 48-49 0.8891742864605305 1103 50-51 1.1059162481951148 1103 52-53 0.9097282170651617 1103 54-55 0.8591359459588439 1103 56-57 0.9349069203609339 1103 58-59 1.0859774421203134 1103 60-61 1.001132593780678 1103 62-63 1.3702979083515068 1103 64-65 1.275836122196754 1103 66-67 1.222663025973624 1103 68-69 1.352053182409584 1103 70-71 1.14067301485629 1103 72-73 1.2614233761412983 1103 74-75 1.466960110244436 1103 76-77 1.3655848123638101 1103 78-79 1.1831900980349133 1103 80-81 1.129924281261662 1103 82-83 1.1915265809473112 1103 84-85 1.0477656047361457 1103 86-87 1.1516029785820514 1103 88-89 1.3214902166035252 1103 90-91 1.6461742289952248 1103 92-93 1.3008109611701109 1103 94-95 1.3511062455325913 1103 96-97 1.5091951365748528 1103 98-99 1.8327029245562976 1103 100-101 2.1123226316080412 1104 1 -0.027875108147583205 1104 2 0.028816728948882542 1104 3 0.09893295468774 1104 4 0.12734536120213136 1104 5 0.007316337230214742 1104 6 0.060263190710230674 1104 7 0.10873810939730078 1104 8 0.07099351014647226 1104 9 0.1778687778807111 1104 10-11 0.053820274345987684 1104 12-13 0.28308631516659233 1104 14-15 0.25141040526770553 1104 16-17 -0.04491776773144096 1104 18-19 0.06709307095224659 1104 20-21 0.030145236915892326 1104 22-23 0.041959033496887344 1104 24-25 0.202612811378863 1104 26-27 0.0844279935695269 1104 28-29 0.1826392320259913 1104 30-31 0.1374346041474226 1104 32-33 0.10136946304450589 1104 34-35 0.2776389389128866 1104 36-37 0.23379928487039336 1104 38-39 0.5909834302109331 1104 40-41 0.6683939047329872 1104 42-43 0.9652077243550892 1104 44-45 0.8852049449291073 1104 46-47 0.9443348240050184 1104 48-49 1.0855572651839367 1104 50-51 1.096088395197043 1104 52-53 1.1719235748988162 1104 54-55 1.3084802399627087 1104 56-57 1.298661272391886 1104 58-59 1.291780150050677 1104 60-61 1.5804614138967317 1104 62-63 1.4434487787576948 1104 64-65 1.245834187960778 1104 66-67 1.4449009369987706 1104 68-69 1.541937128250975 1104 70-71 1.2724428407750494 1104 72-73 1.3285568384236974 1104 74-75 1.2976661063759636 1104 76-77 1.4377704990175815 1104 78-79 1.2235034442631516 1104 80-81 1.2636496197529574 1104 82-83 1.254760623500502 1104 84-85 1.332752065084307 1104 86-87 1.2865836362222822 1104 88-89 1.364675903257293 1104 90-91 1.2662013082988999 1104 92-93 1.672925081092739 1104 94-95 1.3107696884726252 1104 96-97 1.242220281642549 1104 98-99 1.4224920928348261 1104 100-101 1.593471567778252 1105 1 0.03848079127214632 1105 2 0.03785347943244233 1105 3 0.1281399179372542 1105 4 0.20918675385203755 1105 5 0.16035695618572987 1105 6 0.14905235898876157 1105 7 0.20273810939730197 1105 8 0.10748093761262112 1105 9 0.02675465795807952 1105 10-11 0.18011621245043585 1105 12-13 0.07778844282616149 1105 14-15 0.09858642074159718 1105 16-17 0.20729499822600417 1105 18-19 0.06399249068146418 1105 20-21 -0.12802110737811745 1105 22-23 -0.057580618340629997 1105 24-25 -0.1275129139596345 1105 26-27 0.0889115525637294 1105 28-29 -0.010001000082326073 1105 30-31 0.07964737010486544 1105 32-33 0.2116344533733283 1105 34-35 0.10558284606955937 1105 36-37 0.049656151408107974 1105 38-39 0.19974745342176448 1105 40-41 0.3230960323925558 1105 42-43 0.3752096585910607 1105 44-45 0.7197252544068604 1105 46-47 0.6962903365775475 1105 48-49 0.9731742864605337 1105 50-51 0.8529162481951147 1105 52-53 1.1607282170651594 1105 54-55 1.1441359459588405 1105 56-57 1.3139069203609388 1105 58-59 0.8979774421203146 1105 60-61 0.942132593780677 1105 62-63 1.1132979083515053 1105 64-65 1.409836122196758 1105 66-67 1.4746630259736229 1105 68-69 1.6270531824095826 1105 70-71 1.7096730148562926 1105 72-73 1.7214233761412991 1105 74-75 1.7909601102444377 1105 76-77 1.588584812363809 1105 78-79 1.5071900980349113 1105 80-81 1.5649242812616606 1105 82-83 1.4335265809473121 1105 84-85 1.5797656047361457 1105 86-87 1.6946029785820542 1105 88-89 1.8804902166035227 1105 90-91 2.156174228995223 1105 92-93 2.0918109611701077 1105 94-95 2.2411062455325883 1105 96-97 2.490195136574851 1105 98-99 2.6937029245562947 1105 100-101 2.9343226316080404 1106 1 -0.18534041454906003 1106 2 0.01727967485863502 1106 3 0.04851413831147511 1106 4 -0.08897540831012662 1106 5 -0.09483223300345855 1106 6 0.014183336119735657 1106 7 -0.03471303405384418 1106 8 -0.06331324118155379 1106 9 0.02034717355059712 1106 10-11 -0.165902498568272 1106 12-13 -0.2211159230782016 1106 14-15 0.0381498303050094 1106 16-17 0.10034697327797915 1106 18-19 0.12777419546316793 1106 20-21 0.056378061021057135 1106 22-23 0.16147551471549804 1106 24-25 -0.05417611562283042 1106 26-27 0.19249991015207968 1106 28-29 0.18508216000083166 1106 30-31 0.06752262998012526 1106 32-33 0.22608143882031584 1106 34-35 0.155954987441703 1106 36-37 -0.08509228934033075 1106 38-39 0.22535452202883022 1106 40-41 0.2899712922678148 1106 42-43 0.5155360619174658 1106 44-45 0.8819477076293154 1106 46-47 0.723373496660713 1106 48-49 0.8523551596414052 1106 50-51 0.7842260195048851 1106 52-53 0.7374101297470794 1106 54-55 0.3541941580170551 1106 56-57 0.5261189785729918 1106 58-59 0.8161229722658447 1106 60-61 0.7668477704958541 1106 62-63 0.8546367856903849 1106 64-65 0.6184078477684807 1106 66-67 0.9021245644351623 1106 68-69 0.9296207499771505 1106 70-71 0.7968580460413222 1106 72-73 0.9130741037920238 1106 74-75 0.8425838108681383 1106 76-77 0.9129028996818995 1106 78-79 0.9393356281804444 1106 80-81 0.9092340525714349 1106 82-83 0.8718924853132144 1106 84-85 1.0779818209523633 1106 86-87 1.0726112945903665 1106 88-89 1.0237999879132964 1106 90-91 0.9046399254609163 1106 92-93 1.2191456805048269 1106 94-95 1.2269440833704266 1106 96-97 1.0463718517515694 1106 98-99 1.0257445045978777 1106 100-101 1.5705804278658384 1107 1 -0.051808365354361285 1107 2 -0.14802603863984842 1107 3 -0.10358698969326241 1107 4 -0.02119075618812616 1107 5 -0.1124703530512221 1107 6 -0.039252861894773616 1107 7 -0.09407313558261876 1107 8 -0.1033945643954084 1107 9 -0.13580759103790285 1107 10-11 -0.3374139080314933 1107 12-13 -0.24677782223407974 1107 14-15 -0.3011324547604062 1107 16-17 -0.0032190579988977674 1107 18-19 -0.14327256955950674 1107 20-21 -0.027683757980526025 1107 22-23 0.18203383949069263 1107 24-25 0.09025817037772299 1107 26-27 0.050775006378984244 1107 28-29 0.03604317662449574 1107 30-31 -5.855616220387105E-4 1107 32-33 0.163325216425541 1107 34-35 -0.1330075153762209 1107 36-37 -0.1403277843348647 1107 38-39 0.16076351767879515 1107 40-41 -0.05926139732631697 1107 42-43 -0.14180640566596736 1107 44-45 0.014066619868707164 1107 46-47 -9.666915348987004E-4 1107 48-49 0.16592127441234084 1107 50-51 0.3010688586368815 1107 52-53 0.4713145624466826 1107 54-55 0.3951479941516176 1107 56-57 0.28718402879466964 1107 58-59 0.38035495216047366 1107 60-61 0.46764263394132044 1107 62-63 0.33171557903423476 1107 64-65 0.39082809006823993 1107 66-67 0.6568357167366727 1107 68-69 0.6702861141364878 1107 70-71 0.5799300429687406 1107 72-73 0.7476723721252334 1107 74-75 0.5475826002042758 1107 76-77 0.5374322019220443 1107 78-79 0.5397121863883285 1107 80-81 0.46924958246648174 1107 82-83 0.37716513516418004 1107 84-85 0.7553399019249021 1107 86-87 0.6250768741643782 1107 88-89 0.7243777668043272 1107 90-91 0.7844794498787557 1107 92-93 0.9214977081580606 1107 94-95 1.2284636752514615 1107 96-97 1.0942473454101922 1107 98-99 0.8814458964438465 1107 100-101 1.1076559649413724 1108 1 -0.26261493174211026 1108 2 -0.3370059910359906 1108 3 -0.41014928776538895 1108 4 -0.10400265551660226 1108 5 -0.23761860389573997 1108 6 -0.14421037013547533 1108 7 -0.22731077043976455 1108 8 -0.29796101758086024 1108 9 -0.25491947646147395 1108 10-11 -0.3489774739039433 1108 12-13 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0.11725771954450437 2316 6 0.021785183416241694 2316 7 0.345379330771344 2316 8 0.5734962047881993 2316 9 0.35208290223288685 2316 10-11 0.6100017086336464 2316 12-13 0.7020403512231113 2316 14-15 0.4797314589095407 2316 16-17 0.8123178989893631 2316 18-19 0.9687940173990128 2316 20-21 0.6322537017821901 2316 22-23 0.4253659465448649 2316 24-25 0.6055023532159396 2316 26-27 0.5295680411133432 2316 28-29 0.6891211373222461 2316 30-31 0.761845843387313 2316 32-33 0.3665886518466124 2316 34-35 0.033666815535205785 2316 36-37 0.012144701026421956 2316 38-39 -0.045542622914112485 2316 40-41 -0.6721940439433212 2316 42-43 -0.21985141011122522 2316 44-45 -0.4567709287992372 2316 46-47 -1.784037907697254 2316 48-49 -2.2803676982722934 2316 50-51 -1.8458623777590866 2316 52-53 -2.812676363087512 2316 54-55 -2.7815281761785577 2316 56-57 -3.322169415516928 2316 58-59 -3.2102057639865578 2316 60-61 -3.226661299349093 2316 62-63 -3.3891143053889543 2316 64-65 -3.370522656429202 2316 66-67 -3.5218331572324857 2316 68-69 -3.760084222170569 2316 70-71 -3.8679147714032496 2316 72-73 -3.5893857841640475 2316 74-75 -3.6481162256334265 2316 76-77 -3.731667096033135 2316 78-79 -3.838901505018523 2316 80-81 -3.831541367593303 2316 82-83 -3.948503953403833 2316 84-85 -3.955028288393624 2316 86-87 -4.453320685540085 2316 88-89 -4.220792226144571 2316 90-91 -4.2231234809284395 2316 92-93 -4.042173771654319 2316 94-95 -3.8628021514139768 2316 96-97 -3.7413239473946156 2316 98-99 -3.4817779914742353 2316 100-101 -2.859265154651503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 6.0 5 18.0 6 41.0 7 91.0 8 271.0 9 508.0 10 990.0 11 1709.0 12 2717.0 13 3880.0 14 4999.0 15 7380.0 16 10590.0 17 14762.0 18 11639.0 19 5325.0 20 3696.0 21 3309.0 22 3357.0 23 3607.0 24 3875.0 25 4272.0 26 4641.0 27 5204.0 28 6407.0 29 7708.0 30 9261.0 31 10906.0 32 13431.0 33 16978.0 34 22873.0 35 32596.0 36 45090.0 37 75756.0 38 67376.0 39 6114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.485790872980154 22.33787146474536 16.121271974099326 18.055065688175155 2 15.657967660699876 23.61323130475366 40.504341400332535 20.22445963421393 3 18.3415656266104 27.795414547823466 35.07054167841551 18.79247814715062 4 10.92302157573872 20.943245792293407 39.4965336859607 28.637198946007175 5 9.396907968301814 41.853031260634936 34.94409062180952 13.805970149253731 6 23.958647311054285 41.81259540871392 18.934436590274874 15.294320689956924 7 22.814228903127947 41.25035246531255 19.69454626775696 16.24087236380254 8 26.352980641146555 34.12885159508785 20.180462240026447 19.337705523739146 9 27.393117932462786 15.871877643489842 22.441344910400886 34.29365951364648 10-11 23.30409079528522 29.039687690665968 28.09228293553272 19.563938578516094 12-13 25.116563432872102 29.6129461985006 28.520361528962184 16.750128839665116 14-15 21.817845926473307 28.865815240113957 31.080269721041937 18.236069112370803 16-17 16.391946289147956 32.465458399369936 31.31046603206705 19.832129279415053 18-19 16.278063531264888 28.830933328147633 38.64137991385262 16.249623226734858 20-21 17.6095316343695 26.751874143144672 39.416803601466256 16.221790621019572 22-23 17.568579311350682 27.41106262989657 38.87477666905696 16.145581389695785 24-25 17.128136816044357 27.721146518646183 39.519597845810914 15.631118819498546 26-27 16.799085043104338 27.935111957033005 40.0664340381467 15.199368961715956 28-29 15.976133581931725 28.902723319226105 39.72854319559135 15.39259990325082 30-31 17.04870851220022 27.48963260781845 39.99601347639443 15.4656454035869 32-33 15.990014293076124 27.99607669645201 40.07421217925656 15.9396968312153 34-35 16.535241669664646 28.752054021993835 38.778233686933014 15.9344706214085 36-37 17.196053360817526 28.07532062189466 38.297057765418536 16.431568251869283 38-39 15.867048791396845 29.570182603115335 38.274823522548274 16.28794508293954 40-41 16.750286226837506 29.254813594012454 37.85924202757987 16.135658151570176 42-43 16.546874525177014 28.87434284498739 37.68730057434588 16.89148205548971 44-45 17.689647838108836 29.29282002977728 36.40205402449029 16.61547810762359 46-47 17.949356811574475 29.784169688955437 35.16145342110125 17.10502007836884 48-49 17.686709383842018 29.780645035829924 35.146747109784435 17.385898470543623 50-51 17.497593512693612 30.181118554746373 33.825852966056374 18.49543496650364 52-53 18.28590041517983 31.26622555835367 32.628443221484346 17.819430804982158 54-55 18.57334195455385 29.869612142308476 32.4536690423639 19.10337686077377 56-57 18.81289682732603 31.71798885723453 31.60580765603275 17.863306659406692 58-59 19.28143261349383 31.273761023656498 31.194395558450903 18.25041080439877 60-61 18.63374721673943 30.1884109405233 32.8276468929575 18.35019494977977 62-63 18.55681254679281 29.976445593940515 32.77271092010462 18.694030939162058 64-65 18.012922107596722 30.171638453236362 32.88793061538013 18.92750882378679 66-67 18.281403443950722 31.02837224226278 31.454733730687334 19.235490583099164 68-69 18.363078164652347 29.88735694769901 31.869305862105847 19.880259025542795 70-71 18.278000330588153 30.61282779274067 32.59161667331084 18.51755520336033 72-73 18.387386117242116 30.07136814880356 33.0431368926658 18.498108841288516 74-75 17.683792429531245 31.40903477981857 32.060487909224385 18.84668488142581 76-77 17.46708703219345 30.927737318541134 32.26090697832703 19.344268670938384 78-79 17.291826694021218 31.29259968691357 31.403565490485867 20.012008128579346 80-81 17.074270518342782 30.00841055159606 32.31256137758029 20.60475755248087 82-83 16.516341688412233 31.025446872952806 31.98865300635777 20.46955843227719 84-85 16.720043073849045 30.661920116484133 31.442086699490623 21.1759501101762 86-87 16.28012970723502 30.753935457524285 31.11235621846043 21.85357861678027 88-89 16.678658589944288 30.342887979231286 31.383997588651102 21.59445584217333 90-91 16.44945715855443 29.157854305537324 32.49561205190663 21.897076484001616 92-93 16.08338316584747 28.508695166003044 33.032028936890164 22.375892731259327 94-95 15.936172178089784 28.545436424980796 33.08834968448278 22.430041712446645 96-97 16.873608369714237 28.363491399846374 31.957057570954913 22.805842659484476 98-99 16.565039044696302 28.227036553128134 31.5781349700708 23.62978943210477 100-101 16.252435610540555 27.360035201637555 32.75101526822408 23.636513919597807 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1394.0 1 900.5 2 481.5 3 567.5 4 477.5 5 485.5 6 841.5 7 1467.5 8 1998.0 9 2119.5 10 1790.5 11 1414.5 12 1249.5 13 1191.5 14 1219.5 15 1266.0 16 1385.5 17 1602.5 18 1902.5 19 2189.5 20 2527.5 21 2886.5 22 3211.0 23 3601.0 24 4183.0 25 5226.0 26 7544.0 27 10911.0 28 11678.0 29 11887.0 30 12767.0 31 13142.5 32 13656.0 33 13832.5 34 15946.5 35 18212.0 36 19691.0 37 20523.0 38 17365.5 39 13406.5 40 12134.5 41 11433.0 42 10534.5 43 10214.0 44 10352.0 45 9987.0 46 9360.5 47 9401.0 48 10673.0 49 10355.0 50 8821.0 51 7717.5 52 6627.5 53 5776.5 54 4898.5 55 4245.5 56 3581.5 57 2955.5 58 2568.5 59 2306.5 60 2109.5 61 1997.0 62 1633.5 63 1092.5 64 724.0 65 552.5 66 400.0 67 288.5 68 251.0 69 217.5 70 174.0 71 152.0 72 122.0 73 84.0 74 49.0 75 36.0 76 27.0 77 19.0 78 19.0 79 16.0 80 9.0 81 6.0 82 7.5 83 6.0 84 2.0 85 1.0 86 1.5 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06781918772545625 2 0.0 3 0.0 4 0.0 5 0.0019446362071815415 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.4307952589769268E-4 12-13 0.005833908621544624 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.29238577693078E-4 24-25 8.507783406419244E-4 26-27 6.076988147442318E-4 28-29 0.00364619288846539 30-31 4.8615905179538537E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.723181035907707E-4 40-41 2.4307952589769268E-4 42-43 0.012275516057833481 44-45 1.2153976294884634E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.64619288846539E-4 84-85 3.64619288846539E-4 86-87 0.0 88-89 0.0 90-91 0.008264703880521551 92-93 0.002430795258976927 94-95 0.0 96-97 0.0 98-99 1.2153976294884634E-4 100-101 0.010573959376549633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 411388.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.996853115988635 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2226491598315 30.83891898601159 2 13.104103608906904 10.744540217421058 3 4.721894507460839 5.807484466546966 4 2.266236573175011 3.716342716661495 5 1.1994383544022582 2.4586599018556243 6 0.7336609659680202 1.8046674515235155 7 0.4838395597410348 1.388512955368547 8 0.33115017727648494 1.086089214171012 9 0.2130717337298465 0.7861743513802403 >10 1.3565760470562902 10.724256760278443 >50 0.19494805451067843 5.583890996775756 >100 0.14754427225884173 12.21211973805421 >500 0.015406229231846926 4.150629146911624 >1k 0.008295661894071422 5.398781584979744 >5k 0.0011850945562959176 3.298931512060157 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7358 1.7885791515552227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6222 1.5124408101354438 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4307 1.0469435180413624 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2142 0.5206763444728577 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1710 0.41566598928505455 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 1488 0.3617023345357667 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1426 0.34663140393010977 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1396 0.339339018153179 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1394 0.3388528591013836 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1363 0.3313173937985551 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1337 0.3249973261252151 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1246 0.3028770892685251 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1245 0.3026340097426274 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1124 0.27322138710900656 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 1030 0.25037191167462347 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1016 0.24696879831205576 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 973 0.236516378698455 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 934 0.22703627718844496 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 891 0.21658385757484416 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 866 0.21050686942740188 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 852 0.20710375606483417 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 816 0.19835289313251725 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 703 0.17088490670607795 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 664 0.16140480519606795 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 664 0.16140480519606795 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 647 0.15727245325580716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 634 0.15411241941913717 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 626 0.15216778321195562 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 613 0.1490077493752856 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 593 0.14414615885733176 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 583 0.14171536359835485 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 578 0.14049996596886638 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 565 0.13733993213219636 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 564 0.13709685260629867 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 562 0.13661069355450328 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 561 0.13636761402860562 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 543 0.13199218256244713 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 540 0.13126294398475405 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 534 0.12980446682936791 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 533 0.1295613873034702 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 525 0.12761675109628864 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 522 0.1268875125185956 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 500 0.12153976294884636 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 491 0.1193520472157671 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 487 0.11837972911217633 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 475 0.11546277480140403 No Hit GAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGA 465 0.11303197954242711 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 456 0.11084426380934788 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 433 0.10525343471370094 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 429 0.10428111661011016 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 428 0.10403803708421246 No Hit GCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTA 419 0.10185032135113323 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 416 0.10112108277344016 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.4307952589769268E-4 0.0 16-17 0.0 0.0 0.0 2.4307952589769268E-4 0.0 18-19 0.0 0.0 0.0 6.076988147442318E-4 0.0 20-21 0.0 0.0 0.0 9.723181035907707E-4 0.0 22-23 0.0 0.0 0.0 0.002795414547823466 0.0 24-25 0.0 0.0 0.0 0.0049831302809027 0.0 26-27 0.0 0.0 0.0 0.007413925539879627 0.0 28-29 0.0 0.0 0.0 0.013126294398475406 0.0 30-31 0.0 0.0 0.0 0.027346446663490426 0.0 32-33 0.0 0.0 0.0 0.04375431466158468 0.0 34-35 0.0 0.0 0.0 0.06113450076326971 0.0 36-37 0.0 0.0 0.0 0.08386243643470398 0.0 38-39 0.0 0.0 0.0 0.11874434840102288 0.0 40-41 0.0 0.0 0.0 0.1836465818157068 0.0 42-43 0.0 0.0 0.0 0.24429492352718113 0.0 44-45 0.0 0.0 0.0 0.31600338366700054 0.0 46-47 0.0 0.0 0.0 0.4274553462910926 0.0 48-49 0.0 0.0 0.0 0.5182455492138808 0.0 50-51 0.0 0.0 0.0 0.6510885101169699 0.0 52-53 0.0 0.0 0.0 0.7782191021614631 0.0 54-55 0.0 0.0 0.0 0.9431485604830476 0.0 56-57 0.0 0.0 0.0 1.2479702859587543 0.0 58-59 0.0 0.0 0.0 1.5506042957013815 0.0 60-61 0.0 0.0 0.0 1.852509066866316 0.0 62-63 0.0 0.0 0.0 2.162557002148823 0.0 64-65 0.0 0.0 0.0 2.464461773313757 0.0 66-67 0.0 0.0 0.0 2.8779400468657323 0.0 68-69 0.0 0.0 0.0 3.399102550390386 0.0 70-71 0.0 0.0 0.0 3.7723511624062924 0.0 72-73 0.0 0.0 0.0 4.146207473236944 0.0 74-75 0.0 0.0 0.0 4.676242379456863 0.0 76-77 0.0 0.0 0.0 5.317000009723181 0.0 78-79 0.0 0.0 0.0 5.946697521561154 0.0 80-81 0.0 0.0 0.0 6.575544255058485 0.0 82-83 0.0 0.0 0.0 7.271237858177681 0.0 84-85 0.0 0.0 0.0 8.042043034799265 0.0 86-87 0.0 0.0 0.0 8.85259171390512 0.0 88-89 0.0 0.0 0.0 9.889082812332882 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 310 0.0 52.09064 9 GGCATTC 320 0.0 50.46281 7 GCATTCC 350 0.0 46.13743 8 TTAGGCA 355 0.0 44.1551 4 GGTATCA 3895 0.0 41.97179 1 TAGGCAT 375 0.0 41.800163 5 AGGCATT 375 0.0 41.795082 6 CCTTTAG 420 0.0 40.734253 1 GTATCAA 6230 0.0 34.784306 1 CTTTAGG 465 0.0 33.70981 2 TTACGAC 30 0.003947881 31.670639 32-33 GTCGGGA 90 2.4091607E-5 31.66679 2 TTACGGC 30 0.0039502312 31.666786 82-83 TTTAGGC 580 0.0 29.482874 3 AATAAGG 100 4.945354E-5 28.500109 2 CTTACAC 100 4.945354E-5 28.500109 3 ATGGGCG 85 6.399988E-4 27.941282 5 TATCAAC 7835 0.0 27.645288 2 ATCAACG 7955 0.0 27.16855 3 CGGGAGT 105 6.8950554E-5 27.142962 4 >>END_MODULE