FastQCFastQC Report
Thu 26 May 2016
SRR937487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039668
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64670.31706140411086514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48380.23719546514432743No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34700.17012572634369907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34490.16909614702000522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55400.033.977181
GTATCAA85200.033.9765741
TATCAAC106000.027.4318622
ATCAACG105700.027.3292433
TCAACGC106150.027.2588164
CAACGCA107500.026.9620195
GTACATG85950.026.1034581
AACGCAG111000.026.0639326
TACATGG86850.025.0556322
GTATAGG5300.023.3184361
ACATGGG88650.023.206423
ACGCAGA124400.022.838027
CGCAGAG125800.022.508338
GAGTACT62950.020.90247212-13
AGAGTAC103550.020.53023310-11
AGTACTT64650.020.49978412-13
GCAGAGT135350.020.0777669
GAGTACA66150.019.5452381
GTACTTT66850.019.46770514-15
CATGGGA49750.019.100584
Produced by FastQC (version 0.11.5)