##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937487_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2039668 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.393532672964422 31.0 31.0 34.0 30.0 34.0 2 31.491441254164894 33.0 31.0 34.0 30.0 34.0 3 31.52837275478166 33.0 31.0 34.0 28.0 34.0 4 35.16149294885246 37.0 35.0 37.0 33.0 37.0 5 35.16017802897334 37.0 35.0 37.0 33.0 37.0 6 35.18511542074494 37.0 35.0 37.0 32.0 37.0 7 35.10582555592381 37.0 35.0 37.0 32.0 37.0 8 35.08165446533455 37.0 35.0 37.0 32.0 37.0 9 36.630675678590826 39.0 37.0 39.0 32.0 39.0 10-11 36.4863225289606 39.0 37.0 39.0 32.0 39.0 12-13 36.31802651215786 39.0 37.0 39.0 32.0 39.0 14-15 37.35271181388343 40.0 37.0 41.0 32.0 41.0 16-17 37.25854820490393 40.0 37.0 41.0 32.0 41.0 18-19 37.153122468950826 39.5 37.0 41.0 31.0 41.0 20-21 36.99526908300763 39.0 36.0 41.0 31.0 41.0 22-23 37.06814761029736 39.0 37.0 41.0 31.0 41.0 24-25 37.019873577464566 39.0 36.0 41.0 31.0 41.0 26-27 36.91691662564692 39.0 36.0 41.0 30.5 41.0 28-29 36.7662045980032 39.0 36.0 41.0 30.0 41.0 30-31 36.495475244010294 39.0 36.0 40.5 30.0 41.0 32-33 36.23901782054727 39.0 35.0 40.0 30.0 41.0 34-35 36.22389207459253 39.0 35.0 40.0 30.0 41.0 36-37 36.055986562518996 39.0 35.0 40.0 29.0 41.0 38-39 35.88102107794013 39.0 35.0 40.0 28.5 41.0 40-41 35.62562657255985 38.5 35.0 40.0 27.0 41.0 42-43 35.3269745370325 38.0 35.0 40.0 26.0 41.0 44-45 35.065441532641586 38.0 34.0 40.0 25.5 41.0 46-47 34.66077199818794 38.0 34.0 40.0 24.0 41.0 48-49 34.294772237442565 38.0 33.0 40.0 23.0 41.0 50-51 33.311485251521326 37.5 32.0 39.5 21.5 40.5 52-53 33.40814755146425 37.5 33.0 39.5 22.0 40.0 54-55 33.66979233875317 38.0 33.0 40.0 20.5 41.0 56-57 33.33956874354061 37.5 32.5 40.0 18.5 41.0 58-59 33.00939442105284 37.0 32.0 40.0 17.0 41.0 60-61 32.61350229547162 37.0 31.5 40.0 13.5 41.0 62-63 32.17062556259156 36.0 31.0 39.0 9.0 41.0 64-65 31.692582812496937 36.0 31.0 39.0 7.5 40.0 66-67 31.206510569367172 35.0 30.0 39.0 4.5 40.0 68-69 30.655993279298396 35.0 29.0 38.0 2.0 40.0 70-71 30.134454234708787 34.5 29.0 37.5 2.0 40.0 72-73 29.593369607210583 34.0 28.0 37.0 2.0 39.0 74-75 28.994421396031118 34.0 27.5 36.0 2.0 39.0 76-77 28.417160783029395 34.0 26.0 36.0 2.0 38.5 78-79 27.810581673095818 33.5 26.0 35.0 2.0 37.5 80-81 27.15804361298015 33.0 25.5 35.0 2.0 37.0 82-83 26.518516003584896 32.0 24.5 35.0 2.0 36.5 84-85 25.843082060413753 32.0 21.0 35.0 2.0 36.0 86-87 25.157732042665767 31.5 19.0 34.5 2.0 35.5 88-89 24.506864107295893 31.0 16.5 34.0 2.0 35.0 90-91 23.833808247224546 31.0 7.5 34.0 2.0 35.0 92-93 23.052425198610756 30.0 2.0 34.0 2.0 35.0 94-95 22.15884472374916 29.5 2.0 34.0 2.0 35.0 96-97 21.18220171125889 29.0 2.0 34.0 2.0 35.0 98-99 19.900058735049036 28.0 2.0 34.0 2.0 35.0 100-101 16.95182647372023 21.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2049210203089764 1101 2 0.20524609241839187 1101 3 0.18693367053441534 1101 4 0.05714965986268794 1101 5 0.01527240385216544 1101 6 0.12347458018157909 1101 7 0.2188069734115956 1101 8 0.24688295889882994 1101 9 0.30796511908932445 1101 10-11 0.3792972836464301 1101 12-13 0.47744252812138654 1101 14-15 0.5131919243789085 1101 16-17 0.37227973824734306 1101 18-19 0.4887200580545752 1101 20-21 0.5808122777107343 1101 22-23 0.5587596584156884 1101 24-25 0.49050693257991895 1101 26-27 0.39384542837309056 1101 28-29 0.3233668997462118 1101 30-31 0.43594721671158965 1101 32-33 0.4190361680848014 1101 34-35 0.44780698588948553 1101 36-37 0.4362159586698269 1101 38-39 0.31997179676613996 1101 40-41 0.37918107860716077 1101 42-43 0.46180774525278423 1101 44-45 0.44906766287635946 1101 46-47 0.48877369102196866 1101 48-49 0.44776013796616354 1101 50-51 0.471290662513411 1101 52-53 0.5077090249014873 1101 54-55 0.49781038468223215 1101 56-57 0.4297880247372987 1101 58-59 0.572301241357529 1101 60-61 0.5121505093094498 1101 62-63 0.5398142865220805 1101 64-65 0.5379522764784639 1101 66-67 0.637221858322274 1101 68-69 0.6233274126186821 1101 70-71 0.7211941731795264 1101 72-73 0.649235182141517 1101 74-75 0.7990751269101715 1101 76-77 0.8405445868877166 1101 78-79 0.9310109085593332 1101 80-81 0.9502373601073266 1101 82-83 1.0141783300369909 1101 84-85 1.2505082648448003 1101 86-87 1.0576781652166005 1101 88-89 1.1598731244444167 1101 90-91 1.2060542083628896 1101 92-93 1.5017279978941218 1101 94-95 1.7037394784369866 1101 96-97 1.5661354320412357 1101 98-99 1.7276441751470344 1101 100-101 1.459748485773133 1102 1 0.07440237199401878 1102 2 0.07295772181711158 1102 3 0.015283385597914645 1102 4 0.13451562367294656 1102 5 0.1070699353151241 1102 6 0.04955797120624794 1102 7 -0.15738273914868728 1102 8 -0.10907958671643314 1102 9 -1.063216815637702E-4 1102 10-11 -0.035696138890081386 1102 12-13 0.06783917068155176 1102 14-15 0.2052685254273925 1102 16-17 0.2542336295112406 1102 18-19 0.3054978049767314 1102 20-21 0.24046250528184032 1102 22-23 0.19965094709007047 1102 24-25 0.2617314098704 1102 26-27 0.4114798100589283 1102 28-29 0.30589248927337565 1102 30-31 0.34655277359262726 1102 32-33 0.2636307186334932 1102 34-35 0.38196390369921573 1102 36-37 0.4255561654199056 1102 38-39 0.4444556060366409 1102 40-41 0.5055735908592354 1102 42-43 0.5186028060926944 1102 44-45 0.45574388609553296 1102 46-47 0.39095083795428565 1102 48-49 0.309881705657979 1102 50-51 0.3047185183646235 1102 52-53 0.2843004255687518 1102 54-55 0.31674891636291846 1102 56-57 0.41489205817611463 1102 58-59 0.530080379390391 1102 60-61 0.5638373777197572 1102 62-63 0.6213267250335477 1102 64-65 0.4845811471755752 1102 66-67 0.621814036029825 1102 68-69 0.6030209119465937 1102 70-71 0.6646215987903084 1102 72-73 0.8795436645270804 1102 74-75 0.7755137270382058 1102 76-77 0.7906102311466796 1102 78-79 0.8326749611894719 1102 80-81 0.8787528481107891 1102 82-83 0.9560253391489617 1102 84-85 0.7720123984122864 1102 86-87 1.0741654346631435 1102 88-89 1.0593265887272878 1102 90-91 1.1843707427632175 1102 92-93 1.2021520454656773 1102 94-95 1.1121097407402338 1102 96-97 0.8756861176228377 1102 98-99 1.1994721624171305 1102 100-101 1.0241287505144108 1103 1 0.004208400642834675 1103 2 0.06079887173812537 1103 3 -0.05641699423811275 1103 4 -0.04433791598114567 1103 5 0.011282738318456609 1103 6 0.012621201066117749 1103 7 0.007635858399446249 1103 8 -0.05857921352859563 1103 9 0.02528372177263094 1103 10-11 -0.1271765047520219 1103 12-13 -0.26717895144343373 1103 14-15 -0.16782255082879516 1103 16-17 -0.15083860710466013 1103 18-19 -0.2760677337775377 1103 20-21 -0.3445743954128275 1103 22-23 -0.34124864882603134 1103 24-25 -0.18151556523379497 1103 26-27 -0.28609638229649903 1103 28-29 -0.3916672758598665 1103 30-31 -0.22902030776901228 1103 32-33 -0.30769099762599694 1103 34-35 -0.1399406697296257 1103 36-37 -0.20985613273301595 1103 38-39 -0.11508244501452225 1103 40-41 -0.12255584328287483 1103 42-43 -0.22183915962349943 1103 44-45 -0.13871444525688048 1103 46-47 -0.1624004635390932 1103 48-49 -0.24937953059421147 1103 50-51 -0.1582623821228708 1103 52-53 -0.19103960826745947 1103 54-55 -0.006344524124862971 1103 56-57 -0.274761123867016 1103 58-59 -0.1171330419207024 1103 60-61 -0.10664755516763336 1103 62-63 0.047890599806841294 1103 64-65 -0.1798880081057419 1103 66-67 0.11721492918111664 1103 68-69 0.13315513716857552 1103 70-71 0.13305008825762243 1103 72-73 0.18423404231067408 1103 74-75 0.16563596747424114 1103 76-77 0.07060224816090255 1103 78-79 0.023998372995968964 1103 80-81 0.12024815308184955 1103 82-83 0.22229315286091378 1103 84-85 0.28333623024030885 1103 86-87 0.4088885754140321 1103 88-89 0.4899269218842406 1103 90-91 0.5975526158195734 1103 92-93 0.7392776964507242 1103 94-95 0.8462120226810974 1103 96-97 1.0574615125300362 1103 98-99 1.0361614806126838 1103 100-101 0.9646091881407592 1104 1 0.06177249013878594 1104 2 0.1603157159356705 1104 3 0.09371551684694879 1104 4 0.18579586924896319 1104 5 0.2711220686278182 1104 6 0.2192791186034455 1104 7 0.2541924814054113 1104 8 0.1900289104202315 1104 9 0.28242747081701935 1104 10-11 0.27644143011368527 1104 12-13 0.3011035647382059 1104 14-15 0.41129790684410494 1104 16-17 0.37677625707147655 1104 18-19 0.3586388306177568 1104 20-21 0.30218927616354563 1104 22-23 0.4093527476369374 1104 24-25 0.3833221723942586 1104 26-27 0.31834968314358747 1104 28-29 0.31108092759561856 1104 30-31 0.36380307024433733 1104 32-33 0.47333284162786526 1104 34-35 0.4483729992984564 1104 36-37 0.5860406105522458 1104 38-39 0.5689467838728888 1104 40-41 0.5436717954715249 1104 42-43 0.5191414224059585 1104 44-45 0.49528414044212354 1104 46-47 0.5786466925691585 1104 48-49 0.6745677707665791 1104 50-51 0.49330781052785255 1104 52-53 0.4507634343909217 1104 54-55 0.7146947322840873 1104 56-57 0.8165408612509708 1104 58-59 0.9531180283611391 1104 60-61 0.7621047382934591 1104 62-63 0.8588363339692151 1104 64-65 0.8954664590468013 1104 66-67 0.9779103575486836 1104 68-69 0.9300634879152234 1104 70-71 0.9706526569340426 1104 72-73 1.0746903136526065 1104 74-75 1.018627086683253 1104 76-77 1.0009184909619826 1104 78-79 1.1402134614216344 1104 80-81 1.161029649947455 1104 82-83 1.224095813275774 1104 84-85 1.17341955932649 1104 86-87 1.1690719248865342 1104 88-89 1.240016239452153 1104 90-91 1.4681061681359715 1104 92-93 1.5564688436909258 1104 94-95 1.5220188750331687 1104 96-97 1.5136799137328296 1104 98-99 1.4141617099587904 1104 100-101 0.9943668457525057 1105 1 0.04228091184807781 1105 2 -0.02901262675511873 1105 3 0.0626279726589658 1105 4 0.0844348794381915 1105 5 0.008038787280348458 1105 6 0.047473129832397376 1105 7 0.07225496840894152 1105 8 0.01561427120210368 1105 9 0.05720237204388923 1105 10-11 -0.012831287707065542 1105 12-13 0.06761089474444049 1105 14-15 0.09540530963054294 1105 16-17 0.02688932631664187 1105 18-19 0.12262354677495324 1105 20-21 0.12341510273871847 1105 22-23 0.10436210509170962 1105 24-25 0.12222189167061259 1105 26-27 0.04568358832138841 1105 28-29 0.055897417510358594 1105 30-31 0.02083415778852782 1105 32-33 0.02111434505893328 1105 34-35 0.11646459838350154 1105 36-37 0.1922664371274152 1105 38-39 0.11538835176642692 1105 40-41 0.05442818973228469 1105 42-43 -0.0966560406840884 1105 44-45 0.03611357483596578 1105 46-47 0.16865994896341618 1105 48-49 0.23131969424338905 1105 50-51 0.2408532981552156 1105 52-53 0.3002472148026385 1105 54-55 0.47673959608113137 1105 56-57 0.47584607023412673 1105 58-59 0.2438767619855966 1105 60-61 0.31552046921465404 1105 62-63 0.39555472656642365 1105 64-65 0.4372381371503593 1105 66-67 0.4477699957270964 1105 68-69 0.2967148187532409 1105 70-71 0.47127128866620893 1105 72-73 0.574610775287038 1105 74-75 0.44106444372213716 1105 76-77 0.3334845130039845 1105 78-79 0.4927178802197183 1105 80-81 0.49256141330781844 1105 82-83 0.5762002008823472 1105 84-85 0.6637137992932196 1105 86-87 0.539254395254595 1105 88-89 0.5810543970732809 1105 90-91 0.5239532777221605 1105 92-93 0.5524674920585717 1105 94-95 0.5707699147503682 1105 96-97 0.45667715848225754 1105 98-99 0.615860087999966 1105 100-101 0.5983645583746693 1106 1 0.050416576881463726 1106 2 -0.7316046954213604 1106 3 -0.22156505547587813 1106 4 -0.09017439679172412 1106 5 0.041914042694443765 1106 6 -0.12084048792461033 1106 7 -0.023068305538473055 1106 8 -0.041576276255376854 1106 9 -0.08509488554336286 1106 10-11 -0.5035244764367306 1106 12-13 -0.2817605644815657 1106 14-15 -0.22015581548990326 1106 16-17 -0.12052560968461279 1106 18-19 -0.11819606766081137 1106 20-21 -0.14774924384539645 1106 22-23 -0.10521242115358831 1106 24-25 -0.1660253013370223 1106 26-27 -0.16580440807035757 1106 28-29 -0.034317574528238026 1106 30-31 -0.014799098322619386 1106 32-33 -0.02305520889750312 1106 34-35 0.046253057424991084 1106 36-37 -0.023342353901142587 1106 38-39 0.17751206444521017 1106 40-41 0.29131152272392313 1106 42-43 -0.05410695675791288 1106 44-45 0.06921746259726547 1106 46-47 0.22077809207731747 1106 48-49 0.30727847946319997 1106 50-51 0.15361101866557192 1106 52-53 0.20338974782708874 1106 54-55 0.32004432986959586 1106 56-57 0.10316700701444148 1106 58-59 -0.1609332235950447 1106 60-61 -0.039539292558316674 1106 62-63 0.22330397313755412 1106 64-65 0.3537126025746318 1106 66-67 0.47910306937519564 1106 68-69 0.6045708096232509 1106 70-71 0.4561377899543828 1106 72-73 0.5752821982937881 1106 74-75 0.4738855821001984 1106 76-77 0.6964125665264795 1106 78-79 0.6884291444084418 1106 80-81 0.651218840647207 1106 82-83 0.7459916326363825 1106 84-85 0.5345921504286579 1106 86-87 0.6464087234557283 1106 88-89 0.8805466441022745 1106 90-91 0.7377895461820252 1106 92-93 0.8542225883812904 1106 94-95 0.7860293266316525 1106 96-97 0.7872667123020243 1106 98-99 0.61525102361853 1106 100-101 0.3844573890120593 1107 1 0.03501962875684228 1107 2 0.1174564535363487 1107 3 -0.01936205839679772 1107 4 0.07894126984243854 1107 5 0.023011640480298468 1107 6 -0.025248223490656585 1107 7 0.12588069675089741 1107 8 -0.04349588822325501 1107 9 0.04621191337742658 1107 10-11 0.01518666114378675 1107 12-13 -0.05415448952208379 1107 14-15 0.10931507513574701 1107 16-17 0.12029310743179877 1107 18-19 0.2165245872943089 1107 20-21 0.16066744487909546 1107 22-23 0.22788814399132917 1107 24-25 0.18209548735822523 1107 26-27 0.12711346016775593 1107 28-29 0.23003917261468843 1107 30-31 0.09731303209257902 1107 32-33 0.047556559304851476 1107 34-35 0.061626496543055964 1107 36-37 0.033673081938417226 1107 38-39 0.284355855513418 1107 40-41 0.11623008288144376 1107 42-43 0.04420712821350037 1107 44-45 0.05125542064559596 1107 46-47 0.3381841024886185 1107 48-49 0.2683765880620399 1107 50-51 0.1913827363438827 1107 52-53 0.24407309950325384 1107 54-55 0.4417713537338557 1107 56-57 0.38851468490505425 1107 58-59 0.3470386515065442 1107 60-61 0.4045649968776033 1107 62-63 0.34058866546561006 1107 64-65 0.2586256366644335 1107 66-67 0.5077132401260478 1107 68-69 0.5376731742229843 1107 70-71 0.5945028053019925 1107 72-73 0.689142411999363 1107 74-75 0.6680023516260256 1107 76-77 0.704472963195979 1107 78-79 0.7019212825322718 1107 80-81 0.7092857966197705 1107 82-83 0.757169149435164 1107 84-85 0.5393903096444248 1107 86-87 0.536662005429136 1107 88-89 0.5077631661159927 1107 90-91 0.5285422639392756 1107 92-93 0.6352924977816414 1107 94-95 0.7284119441302472 1107 96-97 0.8062291930884875 1107 98-99 1.1248569197934657 1107 100-101 0.9139037257864153 1108 1 0.048812476190160936 1108 2 0.09595909615187281 1108 3 0.10089324554623857 1108 4 0.14527918902113868 1108 5 0.14239110268570698 1108 6 0.13298305908932662 1108 7 0.1257125906696217 1108 8 0.04386826878359784 1108 9 -0.01609659055691992 1108 10-11 0.027613986198772977 1108 12-13 0.14655994623303314 1108 14-15 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2315 96-97 3.569235654720817 2315 98-99 3.613867984992172 2315 100-101 4.077477660171727 2316 1 -0.11044590838396218 2316 2 -0.13925453754762884 2316 3 -0.2690090089210564 2316 4 -0.3231406759919295 2316 5 -0.3495305249595546 2316 6 -0.2601895736913278 2316 7 0.11608815036629494 2316 8 0.0658086653304224 2316 9 -0.29203912310609326 2316 10-11 -0.3153140676200721 2316 12-13 -0.11346941662825571 2316 14-15 -0.22000686065631925 2316 16-17 -0.4965880622167518 2316 18-19 0.045004870570743094 2316 20-21 0.10040146350616652 2316 22-23 0.011862400146590346 2316 24-25 -0.19458302317398335 2316 26-27 -0.11554594384985961 2316 28-29 0.12129034508764391 2316 30-31 0.19360204623897204 2316 32-33 0.48114403014557894 2316 34-35 0.3695342381770814 2316 36-37 0.06481684019803424 2316 38-39 0.38928630040805956 2316 40-41 0.05988927071073391 2316 42-43 0.31790590117043394 2316 44-45 0.601853512609317 2316 46-47 0.5453302161548521 2316 48-49 0.16909689616526435 2316 50-51 0.22894676469941544 2316 52-53 0.32059766302948844 2316 54-55 0.29767421020921603 2316 56-57 0.5509850025972796 2316 58-59 0.43721467935996117 2316 60-61 0.3494962524784526 2316 62-63 0.5087228120622171 2316 64-65 0.5006351681285537 2316 66-67 0.8766874265431461 2316 68-69 0.8238256584708772 2316 70-71 0.9076679330797504 2316 72-73 0.9943776562747324 2316 74-75 1.3537523594715708 2316 76-77 1.2721128841128966 2316 78-79 1.3795358972114578 2316 80-81 1.0677018423261693 2316 82-83 1.3553212623273083 2316 84-85 1.8395224634728748 2316 86-87 2.0677663180374495 2316 88-89 1.9457334628867642 2316 90-91 2.311612757022992 2316 92-93 2.344226523731212 2316 94-95 2.667376227727477 2316 96-97 2.7058334725711717 2316 98-99 2.832304651036516 2316 100-101 3.325343008250403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10519.0 3 2478.0 4 2465.0 5 2745.0 6 3115.0 7 3909.0 8 4736.0 9 5821.0 10 7189.0 11 9034.0 12 10938.0 13 13240.0 14 14785.0 15 17360.0 16 20413.0 17 24480.0 18 22676.0 19 19417.0 20 20308.0 21 21759.0 22 24235.0 23 27069.0 24 30354.0 25 34392.0 26 39179.0 27 45092.0 28 52346.0 29 60942.0 30 71872.0 31 84876.0 32 103178.0 33 127146.0 34 160639.0 35 205655.0 36 260884.0 37 290548.0 38 169803.0 39 14069.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.38682184764255 18.449423137363492 14.093135145264524 23.07061986972943 2 15.284776038980608 19.547628681287506 40.56592874170922 24.601666538022663 3 19.621610407385305 23.93724183838112 29.084349100877343 27.356798653356236 4 12.489411649029657 15.789683271265808 37.086193970232166 34.63471110947236 5 13.706358890756004 37.25837424546493 34.165210611032066 14.870056252747005 6 32.750538015943654 35.59097715686771 17.336817393039382 14.321667434149257 7 29.3026035400596 32.06635842513741 20.225155890008097 18.405882144794887 8 26.619856956313832 34.94564231804883 19.423146577758686 19.01135414787865 9 26.29464917661492 16.54177283379732 20.071043504930874 37.09253448465689 10-11 25.074421293337867 26.531068186420914 27.210740064216633 21.183770456024586 12-13 25.92198198109637 23.63956294574177 27.919224587496867 22.51923048566499 14-15 22.579814427968646 25.16757092015154 26.142365924219636 26.110248727660178 16-17 22.214782317675787 28.658954611777045 26.67512112847162 22.45114194207555 18-19 22.093404360599816 27.11191216886551 29.040183540064874 21.7544999304698 20-21 22.772581559415883 26.139234736963406 29.23082290752189 21.857360796098817 22-23 22.335606199834697 26.171436967266203 29.16516381641083 22.327793016488272 24-25 22.221512412443598 26.274144043117996 29.31474800169437 22.189595542744033 26-27 22.062670402154613 26.52889290839578 29.166731160588387 22.241705528861214 28-29 21.883817015460693 26.781120056143852 29.198539591394223 22.13652333700123 30-31 22.07468155644739 26.407578256136016 29.21226539133598 22.305474796080617 32-33 21.85796029100435 26.512653725221814 29.155922744513564 22.473463239260276 34-35 22.353088329830754 26.566674464734714 28.996894861728457 22.083342343706075 36-37 22.273305022798066 26.388332973370353 28.98689710194176 22.351464901889816 38-39 21.995191097019013 26.683539838053917 29.03460547906368 22.286663585863387 40-41 22.340000830376447 26.565855362671826 28.947782833465546 22.14636097348618 42-43 22.112084883201813 26.547794344379525 28.79348712484051 22.54663364757815 44-45 22.171251305564983 26.78511979011699 28.62724710122192 22.416381803096108 46-47 22.332329565209914 26.942045598347775 28.271663638161865 22.453961198280446 48-49 22.422023840386526 26.96611680948996 28.106581650092483 22.505277700031037 50-51 22.376440124868576 27.148986536469895 28.08412457562428 22.39044876303725 52-53 22.52545532487248 27.198582013536843 27.892261738552477 22.383700923038198 54-55 22.2695293397782 27.04867314265774 28.013338608489235 22.668458909074822 56-57 22.306066489664225 27.305333142360034 27.897243916226927 22.491356451748818 58-59 22.517106483329545 27.03985064452537 27.957600732038344 22.485442140106734 60-61 22.440941109998832 27.025063103009906 28.35246792647501 22.181527860516248 62-63 22.251711174019782 27.10429384851949 28.29816646853137 22.345828508929355 64-65 22.151586383122144 27.124637141437276 28.143306440182165 22.58047003525842 66-67 22.152592967093117 27.225890389320316 27.910284633225462 22.71123201036111 68-69 22.182110479286333 27.091271916754717 28.12569630860324 22.600921295355718 70-71 22.23650663484079 27.061021274630377 28.002212023047885 22.70026006748095 72-73 22.292774713524153 27.00369716783039 27.957212204265268 22.746315914380187 74-75 22.060681670717532 27.34232639522711 27.87982673610915 22.71716519794621 76-77 22.22449636981342 27.15569739902764 27.824460594987443 22.795345636171493 78-79 22.26027397260274 27.13796617898836 27.837541794679765 22.764218053729138 80-81 22.265846025935634 27.247182108387573 27.776081144861138 22.710890720815655 82-83 22.245975957082116 27.284754024779932 27.864908016787247 22.604362001350705 84-85 22.242307273777268 27.126438306262727 27.764238995573837 22.867015424386167 86-87 22.286705164825772 27.093005774723867 27.79342563851624 22.826863421934124 88-89 22.3339906776182 27.050384739261485 27.63666344914369 22.978961133976625 90-91 22.289252972101032 26.87463287111433 27.8862441551116 22.94987000167304 92-93 22.42529022544864 26.631409503612037 27.904170814616375 23.03912945632295 94-95 22.19760603936673 26.624588225554007 27.969707846199466 23.2080978888798 96-97 22.105942041448102 26.610616316351976 28.012333697775265 23.271107944424653 98-99 22.210630827143223 26.700947821769116 27.705648233482037 23.382773117605623 100-101 22.68093788115701 25.85754416047501 28.241749198226824 23.219768760141157 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3085.0 1 1724.5 2 338.5 3 331.5 4 296.0 5 272.5 6 413.0 7 795.5 8 1318.5 9 1610.0 10 1598.5 11 1623.0 12 1789.5 13 1922.5 14 2081.5 15 2273.5 16 2436.5 17 2671.5 18 2930.5 19 3196.0 20 3558.0 21 4158.0 22 4975.0 23 6100.0 24 7484.5 25 9271.0 26 11742.5 27 14608.5 28 17193.0 29 19993.5 30 23557.0 31 27531.5 32 32048.5 33 35949.0 34 39606.5 35 43347.5 36 47737.5 37 52128.5 38 55009.0 39 57149.5 40 59533.5 41 62957.5 42 65556.5 43 68548.5 44 72342.0 45 74889.5 46 75850.0 47 77535.5 48 80962.5 49 80659.5 50 76732.5 51 73504.0 52 70878.5 53 68955.5 54 66809.0 55 62059.5 56 56194.0 57 50548.5 58 45681.5 59 40894.5 60 34953.0 61 29014.5 62 23704.0 63 18546.0 64 14109.0 65 11062.5 66 8515.5 67 6513.0 68 4933.5 69 3561.0 70 2558.5 71 1794.5 72 1355.0 73 1054.5 74 790.0 75 598.5 76 387.5 77 256.0 78 177.0 79 112.0 80 69.5 81 47.0 82 32.5 83 24.0 84 19.0 85 11.5 86 13.0 87 14.5 88 7.0 89 3.5 90 5.0 91 4.0 92 3.5 93 4.0 94 2.5 95 2.0 96 2.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31941472827930817 2 0.2760743415104811 3 0.2732797690604549 4 0.27421129321046367 5 0.2762214242710088 6 0.23910754103118742 7 0.24023517552856638 8 0.24048031346277926 9 0.2520017963707819 10-11 0.2600423206129625 12-13 0.2519037411970968 14-15 0.24182857210094977 16-17 0.23753865825222537 18-19 0.22459537532578833 20-21 0.2301600064324194 22-23 0.22778216847055502 24-25 0.2301354926389981 26-27 0.2251591925744778 28-29 0.23999003759435358 30-31 0.25312943086816087 32-33 0.24467217213781853 34-35 0.24396127212860133 36-37 0.2322191650798071 38-39 0.21986421319548083 40-41 0.2180011648954634 42-43 0.22962070297715118 44-45 0.22697321328765271 46-47 0.237072896177221 48-49 0.24158343416673692 50-51 0.2553846998629189 52-53 0.2704361690235862 54-55 0.2438141893680736 56-57 0.21961907526126803 58-59 0.20863689580853353 60-61 0.21108827515066178 62-63 0.21780505454809312 64-65 0.20922522685064435 66-67 0.2139809027743731 68-69 0.2148143717506967 70-71 0.2170451269520334 72-73 0.21873657869810187 74-75 0.22094282010601726 76-77 0.23128764092979837 78-79 0.2314102098969048 80-81 0.22511016498763525 82-83 0.2173147786796675 84-85 0.2038321922979622 86-87 0.20817113373352916 88-89 0.21858949593757415 90-91 0.21814824765599106 92-93 0.22189885804944726 94-95 0.21677547522439927 96-97 0.22518370636789908 98-99 0.22716932363502298 100-101 0.2125591027559387 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2039668.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.18738632570883 #Duplication Level Percentage of deduplicated Percentage of total 1 81.43472611919856 52.27082225741289 2 11.202584229504332 14.381292035709755 3 3.2614457706053903 6.28031038974592 4 1.3938259814236709 3.5786418696180573 5 0.7341470962554723 2.356149164362367 6 0.4392409270225418 1.6916236243715026 7 0.27891090344635905 1.2531793336974746 8 0.20998640008217964 1.0782782548175778 9 0.15588265184858582 0.9005129996113032 >10 0.8064128135166049 9.40381815175033 >50 0.05591941483078744 2.420962296547895 >100 0.025465649507814776 2.9204384421687695 >500 9.17079636524456E-4 0.374381049697786 >1k 4.5853981826221276E-4 0.7723571314847328 >5k 7.642330304370214E-5 0.3172329990036054 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6467 0.31706140411086514 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4838 0.23719546514432743 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3470 0.17012572634369907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3449 0.16909614702000522 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.902758684256458E-5 14-15 0.0 0.0 0.0 4.902758684256458E-5 1.2256896710641143E-4 16-17 0.0 0.0 0.0 4.902758684256458E-5 1.4708276052769373E-4 18-19 0.0 0.0 0.0 4.902758684256458E-5 1.4708276052769373E-4 20-21 0.0 0.0 0.0 7.354138026384687E-5 1.4708276052769373E-4 22-23 0.0 0.0 0.0 9.805517368512915E-5 2.696517276341052E-4 24-25 0.0 0.0 0.0 1.7159655394897603E-4 3.922206947405166E-4 26-27 0.0 0.0 0.0 3.677069013192343E-4 3.922206947405166E-4 28-29 2.451379342128229E-5 0.0 0.0 8.089551829023155E-4 3.922206947405166E-4 30-31 4.902758684256458E-5 0.0 0.0 0.0020346448539664296 3.922206947405166E-4 32-33 4.902758684256458E-5 0.0 0.0 0.0035299862526646493 3.922206947405166E-4 34-35 9.805517368512915E-5 0.0 0.0 0.004902758684256457 3.922206947405166E-4 36-37 9.805517368512915E-5 0.0 0.0 0.0070109449184867344 4.4124828158308117E-4 38-39 1.4708276052769373E-4 0.0 0.0 0.0105164173777301 4.4124828158308117E-4 40-41 1.4708276052769373E-4 0.0 0.0 0.015223065714616301 4.4124828158308117E-4 42-43 1.961103473702583E-4 0.0 0.0 0.019022703694915055 4.4124828158308117E-4 44-45 2.4513793421282286E-4 0.0 0.0 0.02135151406993687 4.4124828158308117E-4 46-47 2.4513793421282286E-4 0.0 0.0 0.02500406928970793 4.902758684256457E-4 48-49 2.4513793421282286E-4 0.0 0.0 0.03012745211475593 4.902758684256457E-4 50-51 2.4513793421282286E-4 0.0 0.0 0.037432562554298054 4.902758684256457E-4 52-53 2.4513793421282286E-4 0.0 0.0 0.055327631751834125 5.14789661846928E-4 54-55 2.4513793421282286E-4 0.0 0.0 0.06778063880984553 5.393034552682103E-4 56-57 2.4513793421282286E-4 0.0 0.0 0.10050655302725739 5.393034552682103E-4 58-59 2.4513793421282286E-4 0.0 0.0 0.1586042434356964 5.393034552682103E-4 60-61 2.4513793421282286E-4 0.0 0.0 0.21290229586383666 5.393034552682103E-4 62-63 2.4513793421282286E-4 0.0 0.0 0.24148537899305178 5.393034552682103E-4 64-65 2.4513793421282286E-4 0.0 0.0 0.29517058658566003 5.393034552682103E-4 66-67 2.4513793421282286E-4 0.0 0.0 0.39398568786684895 5.393034552682103E-4 68-69 2.4513793421282286E-4 0.0 0.0 0.5376610311089844 5.393034552682103E-4 70-71 2.4513793421282286E-4 0.0 0.0 0.6741048052918417 5.393034552682103E-4 72-73 2.4513793421282286E-4 0.0 0.0 0.8197657658011009 5.883310421107749E-4 74-75 2.4513793421282286E-4 0.0 0.0 0.9895237852434807 5.883310421107749E-4 76-77 2.4513793421282286E-4 0.0 0.0 1.1249134663092228 5.883310421107749E-4 78-79 2.4513793421282286E-4 2.451379342128229E-5 0.0 1.3031777720687878 6.373586289533395E-4 80-81 2.4513793421282286E-4 4.902758684256458E-5 0.0 1.541402816536809 6.373586289533395E-4 82-83 2.4513793421282286E-4 4.902758684256458E-5 0.0 1.7554082331046033 6.373586289533395E-4 84-85 2.4513793421282286E-4 4.902758684256458E-5 0.0 2.0096898122635647 6.86386215795904E-4 86-87 2.4513793421282286E-4 4.902758684256458E-5 0.0 2.3246920577270416 6.86386215795904E-4 88-89 2.4513793421282286E-4 4.902758684256458E-5 0.0 2.736376704444057 6.86386215795904E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5540 0.0 33.97718 1 GTATCAA 8520 0.0 33.976574 1 TATCAAC 10600 0.0 27.431862 2 ATCAACG 10570 0.0 27.329243 3 TCAACGC 10615 0.0 27.258816 4 CAACGCA 10750 0.0 26.962019 5 GTACATG 8595 0.0 26.103458 1 AACGCAG 11100 0.0 26.063932 6 TACATGG 8685 0.0 25.055632 2 GTATAGG 530 0.0 23.318436 1 ACATGGG 8865 0.0 23.20642 3 ACGCAGA 12440 0.0 22.83802 7 CGCAGAG 12580 0.0 22.50833 8 GAGTACT 6295 0.0 20.902472 12-13 AGAGTAC 10355 0.0 20.530233 10-11 AGTACTT 6465 0.0 20.499784 12-13 GCAGAGT 13535 0.0 20.077766 9 GAGTACA 6615 0.0 19.545238 1 GTACTTT 6685 0.0 19.467705 14-15 CATGGGA 4975 0.0 19.10058 4 >>END_MODULE