FastQCFastQC Report
Thu 26 May 2016
SRR937487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2039668
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81820.40114371554586337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65390.32059139036352974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46550.22822341675213811No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22430.10996887728787234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98600.033.107521
GGTATCA64550.032.3894271
ATCAACG121700.026.6176853
TCAACGC121950.026.5631184
TATCAAC123200.026.4092662
CAACGCA124150.026.0924055
AACGCAG127850.025.3366666
GTACATG83000.025.189611
TACATGG83900.024.5699482
ACATGGG84950.023.0920853
ACGCAGA143950.022.408867
CGCAGAG145500.022.0721868
AGTACTT82800.021.11441812-13
CATGGGG39000.021.0696814
GAGTACT80450.020.81587812-13
AGAGTAC121600.020.72590310-11
GCAGAGT157050.020.0556729
GTACTTT83100.019.94757814-15
TACCTGG17600.019.7009262
CGTATAC1250.006034085518.999253
Produced by FastQC (version 0.11.5)