FastQCFastQC Report
Thu 26 May 2016
SRR937486_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937486_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018039
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65710.3256131323527444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48400.23983679205406833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33430.16565586690841952No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29080.14410028745727907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.035.406291
GTATCAA85600.034.005561
ATCAACG104900.027.8238223
TATCAAC106850.027.361212
TCAACGC106450.027.332774
CAACGCA108250.026.8782755
GTACATG82450.026.4785581
AACGCAG110750.026.2915956
TACATGG83500.025.3342632
ACATGGG82450.024.6184753
ACGCAGA125800.022.9951827
CGCAGAG127450.022.5856698
TACCTGG17200.022.386762
GTACCTG18400.021.972191
GAGTACT67950.020.97031812-13
AGAGTAC106500.020.94045810-11
GTGTAGC10350.020.6797081
GCAGAGT136200.020.4022929
AGTACTT71150.019.89365612-13
GAGTACA62200.019.5759051
Produced by FastQC (version 0.11.5)