FastQCFastQC Report
Thu 26 May 2016
SRR937486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018039
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85330.42283622863581927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67930.33661391083125747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48130.2384988595364113No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22220.11010689089754955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69400.032.8695451
GTATCAA104400.031.8646741
TATCAAC128500.025.656422
ATCAACG128550.025.6094863
TCAACGC129750.025.3726374
CAACGCA131050.025.1209415
AACGCAG133800.024.6719726
GTACATG83350.023.719181
TACATGG83800.022.8455052
GTACGGG1503.0229217E-522.1778141
ACATGGG84250.021.7649233
ACGCAGA151150.021.7489197
GAGTACT82100.021.64054312-13
GTCGTAA1100.002869727821.6017671
AGTACTT85700.021.36895612-13
CGCAGAG153300.021.2889528
AGAGTAC123250.020.96774510-11
GTACTTT85050.020.858914-15
GCAGAGT161150.019.8097369
TACTTTT96250.018.87582214-15
Produced by FastQC (version 0.11.5)