##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937486_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2018039 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14322220730125 33.0 31.0 34.0 31.0 34.0 2 32.377044249392604 34.0 31.0 34.0 31.0 34.0 3 32.45708829214896 34.0 31.0 34.0 31.0 34.0 4 36.07477853500354 37.0 35.0 37.0 35.0 37.0 5 35.92138704950697 37.0 35.0 37.0 35.0 37.0 6 36.00441765496108 37.0 35.0 37.0 35.0 37.0 7 35.964519516223426 37.0 35.0 37.0 35.0 37.0 8 35.94236979562833 37.0 35.0 37.0 35.0 37.0 9 37.63957782778232 39.0 37.0 39.0 35.0 39.0 10-11 37.558589056009325 39.0 37.0 39.0 35.0 39.0 12-13 37.46471723291769 39.0 37.0 39.0 35.0 39.0 14-15 38.6878737229558 40.0 38.0 41.0 34.0 41.0 16-17 38.66682457573912 40.0 38.0 41.0 34.0 41.0 18-19 38.664430667593635 40.0 38.0 41.0 34.0 41.0 20-21 38.57005365109396 40.0 38.0 41.0 34.0 41.0 22-23 38.43920583298935 40.0 38.0 41.0 34.0 41.0 24-25 38.29772417678747 40.0 38.0 41.0 34.0 41.0 26-27 38.07433726503799 40.0 37.5 41.0 33.0 41.0 28-29 38.057872766581816 40.0 37.5 41.0 33.0 41.0 30-31 38.191855558787516 40.0 38.0 41.0 34.0 41.0 32-33 38.159977086666814 40.0 38.0 41.0 34.0 41.0 34-35 38.049409352346515 40.0 37.0 41.0 33.0 41.0 36-37 37.89766426714251 40.0 37.0 41.0 33.0 41.0 38-39 37.75619128272546 40.0 37.0 41.0 33.0 41.0 40-41 37.59640646191674 40.0 37.0 41.0 33.0 41.0 42-43 37.39047263209482 40.0 37.0 41.0 32.5 41.0 44-45 37.12153704660812 40.0 36.5 41.0 31.5 41.0 46-47 36.80174218635021 40.0 36.0 41.0 31.0 41.0 48-49 36.48919173514486 39.0 36.0 41.0 30.0 41.0 50-51 36.27136418077153 39.0 35.5 41.0 30.0 41.0 52-53 36.03867938132018 39.0 35.0 40.5 29.5 41.0 54-55 35.82749986496792 39.0 35.0 40.0 29.0 41.0 56-57 35.57443637115041 38.5 35.0 40.0 28.0 41.0 58-59 35.31828844734913 38.0 35.0 40.0 28.0 41.0 60-61 34.97575145970916 38.0 34.0 40.0 27.0 41.0 62-63 34.59956100947504 37.5 34.0 40.0 26.5 41.0 64-65 34.25725196589362 37.0 34.0 40.0 26.0 41.0 66-67 33.86425287122796 36.0 33.0 39.0 26.0 41.0 68-69 33.42088532481285 36.0 33.0 39.0 25.0 40.0 70-71 33.03479541277448 35.0 32.5 38.5 24.5 40.0 72-73 32.61008682190979 35.0 32.0 37.5 23.5 40.0 74-75 32.16933642015839 35.0 32.0 37.0 22.5 39.0 76-77 30.86606849520748 34.0 30.5 35.5 21.0 38.5 78-79 31.492315312043026 34.5 31.0 36.0 22.0 38.5 80-81 31.3287002877546 35.0 31.0 36.0 21.0 37.5 82-83 30.996728011698487 34.0 31.0 35.0 20.0 37.0 84-85 30.668401353987708 34.0 31.0 35.0 19.0 36.5 86-87 30.337531385666978 34.0 31.0 35.0 18.0 36.0 88-89 29.976700896266127 34.0 30.0 35.0 14.5 36.0 90-91 29.531648545939895 34.0 30.0 35.0 7.0 35.5 92-93 29.119102752721826 34.0 29.0 35.0 2.0 35.0 94-95 28.715073147743922 34.0 29.0 35.0 2.0 35.0 96-97 28.289058833848106 34.0 29.0 35.0 2.0 35.0 98-99 27.6471604364435 33.0 28.0 35.0 2.0 35.0 100-101 26.064194249962462 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0939566625128947 1101 2 0.1494121585870971 1101 3 0.09895869226620135 1101 4 0.07114506892687444 1101 5 -0.05703527546527454 1101 6 0.05349994827687965 1101 7 0.06440725060080155 1101 8 0.024226817836748182 1101 9 0.1429339175708222 1101 10-11 0.13790448926872756 1101 12-13 0.07338781882108947 1101 14-15 0.21518527746709282 1101 16-17 0.12907655017804842 1101 18-19 0.1733740330836966 1101 20-21 0.03809133770265305 1101 22-23 0.04732447732835965 1101 24-25 -0.016385512577606676 1101 26-27 -0.07601457292291514 1101 28-29 0.032572457095390916 1101 30-31 0.22231845231997482 1101 32-33 0.21183545524024083 1101 34-35 0.2440518293537366 1101 36-37 0.25726967727802474 1101 38-39 0.15323143080958346 1101 40-41 0.13617826901966623 1101 42-43 0.07586535479361345 1101 44-45 0.22358151433714823 1101 46-47 0.21251052317730057 1101 48-49 0.08316056413533346 1101 50-51 0.214505640524429 1101 52-53 0.44212234802476047 1101 54-55 0.3138518870927953 1101 56-57 0.32957302751334794 1101 58-59 0.27406534535932536 1101 60-61 0.381438870900233 1101 62-63 0.37869715427468975 1101 64-65 0.41497117986799736 1101 66-67 0.3876428951187947 1101 68-69 0.24018583539510274 1101 70-71 0.021972079471346717 1101 72-73 -0.04658903166974682 1101 74-75 0.11094797509354493 1101 76-77 0.08422002217843882 1101 78-79 0.11211265292761041 1101 80-81 0.035859399795885594 1101 82-83 0.04795940681839994 1101 84-85 -0.008739904129640763 1101 86-87 -0.15471799680901555 1101 88-89 -0.10501658099585498 1101 90-91 -0.005000956205254425 1101 92-93 -0.12383924862836793 1101 94-95 -0.2913668321831828 1101 96-97 -0.24743501143259294 1101 98-99 -0.2714804113156255 1101 100-101 -0.12194813361395518 1102 1 0.04083717479480242 1102 2 0.031895899194573474 1102 3 -0.059883241770464224 1102 4 -0.031179361661408223 1102 5 -0.1433447015579361 1102 6 -0.035313820117110595 1102 7 0.01536994119094004 1102 8 -0.042497122563048606 1102 9 -0.004634945028101356 1102 10-11 0.022894802334441522 1102 12-13 -0.0445199570050292 1102 14-15 -0.007572333841579848 1102 16-17 -0.04011696685495991 1102 18-19 -0.0636593081336514 1102 20-21 -0.007570877033948875 1102 22-23 -0.03508415655061725 1102 24-25 -0.0601447839903031 1102 26-27 -0.1193283085459882 1102 28-29 -0.20978656074846214 1102 30-31 -0.19316547747914825 1102 32-33 -0.12044305517849097 1102 34-35 -0.13898399898415192 1102 36-37 -0.14037361680743743 1102 38-39 -0.11959729152981424 1102 40-41 -0.14037283298139158 1102 42-43 -0.07908811484113443 1102 44-45 -0.1255432296129655 1102 46-47 -0.16543391890620995 1102 48-49 0.1502848176987257 1102 50-51 -0.005980617930184451 1102 52-53 0.01567211725927109 1102 54-55 -0.057057958690613475 1102 56-57 -0.11480487703371267 1102 58-59 -0.06617965719978258 1102 60-61 -0.005572274061087512 1102 62-63 -0.018414866878103453 1102 64-65 -0.09132219900679672 1102 66-67 -0.07994586295762218 1102 68-69 -0.1359480290294428 1102 70-71 -0.23907255575613107 1102 72-73 -0.2137672921993996 1102 74-75 -0.3284893265851103 1102 76-77 -0.22516202488505854 1102 78-79 -0.15592340028442564 1102 80-81 -0.03340623803332221 1102 82-83 -0.007157867840664522 1102 84-85 -0.06414009897101636 1102 86-87 -0.10451326221069834 1102 88-89 -0.072526354348728 1102 90-91 -0.112921036302577 1102 92-93 -0.07968208005539523 1102 94-95 -0.14528474085107135 1102 96-97 -0.15504834583805405 1102 98-99 -0.07083443397972289 1102 100-101 0.02133356208831927 1103 1 -0.028434934788784005 1103 2 -0.1004443869407865 1103 3 -0.10946665417388601 1103 4 -0.0029632902929463967 1103 5 -0.1799154531523115 1103 6 -0.1668471034635104 1103 7 -0.09477636571940451 1103 8 -0.0906088555567166 1103 9 -0.19342469208022806 1103 10-11 -0.17129522883340798 1103 12-13 -0.1837745264433721 1103 14-15 -0.22194420345861232 1103 16-17 -0.24251910816253286 1103 18-19 -0.1961529577278469 1103 20-21 -0.1419892227916577 1103 22-23 -0.1461319844849882 1103 24-25 -0.1893531016925678 1103 26-27 -0.21739036763366926 1103 28-29 -0.36278481331972756 1103 30-31 -0.2322105945085795 1103 32-33 -0.048763840555508864 1103 34-35 -0.05826182844545258 1103 36-37 -0.052199140779947584 1103 38-39 -0.022925748128749035 1103 40-41 -0.09104919327563721 1103 42-43 -0.1773901282868664 1103 44-45 -0.0983231975035963 1103 46-47 -0.12699542600739733 1103 48-49 -0.2589516383870105 1103 50-51 -0.1514502796500281 1103 52-53 -0.0027043619206139624 1103 54-55 -0.1485677698053749 1103 56-57 -0.21326360781114317 1103 58-59 -0.2154466957276071 1103 60-61 -0.21168690870359086 1103 62-63 -0.2635574833087091 1103 64-65 -0.32029323325337344 1103 66-67 -0.24060466603235398 1103 68-69 -0.2749148971561439 1103 70-71 -0.2753718265875804 1103 72-73 -0.21588901314839148 1103 74-75 -0.28921907321770135 1103 76-77 -0.4867930120359425 1103 78-79 -0.12511240699656767 1103 80-81 -0.14188634090819008 1103 82-83 -0.4016019762202703 1103 84-85 -0.2992921989344772 1103 86-87 -0.16326256521493931 1103 88-89 -0.3303150227953431 1103 90-91 -0.43525315850160595 1103 92-93 -0.2716548963670711 1103 94-95 -0.22762201459881126 1103 96-97 -0.07270887682808436 1103 98-99 -0.2545841028609992 1103 100-101 0.025562734781797047 1104 1 -0.15634154946582868 1104 2 -0.08873332521027066 1104 3 -0.07603120181444467 1104 4 -0.02493871414080928 1104 5 -0.1655637150610616 1104 6 -0.15094994211818147 1104 7 -0.11400768269277961 1104 8 -0.10110288893158526 1104 9 -0.13415635253462455 1104 10-11 -0.07234595975041458 1104 12-13 -0.15218929752762733 1104 14-15 -0.14500384943419675 1104 16-17 -0.1505752348454763 1104 18-19 -0.1627783929928981 1104 20-21 -0.2152781296819839 1104 22-23 -0.22510438000328037 1104 24-25 -0.2579315754009386 1104 26-27 -0.3046569369123162 1104 28-29 -0.3840064737210227 1104 30-31 -0.21567589326949133 1104 32-33 -0.20514305326705795 1104 34-35 -0.3197586067720124 1104 36-37 -0.11211684774598041 1104 38-39 -0.14912425071559454 1104 40-41 -0.06118999877364928 1104 42-43 -0.07258339731385632 1104 44-45 0.08668925036476338 1104 46-47 -0.016853937409372577 1104 48-49 -0.05865543125757 1104 50-51 0.043812892473305 1104 52-53 0.1180945730226739 1104 54-55 0.13339026757829942 1104 56-57 0.11261588948518408 1104 58-59 0.030192990017418708 1104 60-61 -0.07636117620555893 1104 62-63 -0.05254752839331189 1104 64-65 0.02696144091274988 1104 66-67 -0.002495554443747494 1104 68-69 -0.21873263690130784 1104 70-71 -0.12688097071396243 1104 72-73 0.0012891176099785184 1104 74-75 -0.167458608632451 1104 76-77 -0.16217724975667736 1104 78-79 -0.17153086295882147 1104 80-81 -0.21715720333763855 1104 82-83 -0.21795464216891958 1104 84-85 -0.24385440906955935 1104 86-87 -0.15104418707297995 1104 88-89 -0.1177468515868263 1104 90-91 0.04031304209614461 1104 92-93 0.03859748132785512 1104 94-95 -0.07297040525863707 1104 96-97 -0.027464182197924458 1104 98-99 0.1995727658664883 1104 100-101 0.3513216637196024 1105 1 -0.020496336185246378 1105 2 0.017179070885340764 1105 3 -0.027379781155957517 1105 4 -0.013086386914359593 1105 5 -0.2000557173352533 1105 6 -0.09959248794829278 1105 7 -0.0818423180520611 1105 8 -0.0918703964730625 1105 9 -0.10680597685986726 1105 10-11 -0.047982067020015506 1105 12-13 -0.15330581406885813 1105 14-15 -0.061245198145861934 1105 16-17 -0.08593534345104104 1105 18-19 -0.06796730239547344 1105 20-21 -0.1532539452283359 1105 22-23 -0.12123999320839118 1105 24-25 -0.029348351303262632 1105 26-27 -0.09758940481027167 1105 28-29 -0.22169539313964748 1105 30-31 -0.18847642609912896 1105 32-33 -0.10777155647200942 1105 34-35 -0.0968887810792296 1105 36-37 -0.18248318876349856 1105 38-39 -0.135145121252485 1105 40-41 -0.1598084635885968 1105 42-43 -0.2723267678716965 1105 44-45 -0.20870127789876847 1105 46-47 -0.1282825674600261 1105 48-49 -0.0719217213935579 1105 50-51 -0.03164645880951156 1105 52-53 -0.05694496609813626 1105 54-55 0.029574322049107593 1105 56-57 -0.02680681446256017 1105 58-59 -0.0038128999063005153 1105 60-61 -0.15648217981631518 1105 62-63 -0.11967042922627513 1105 64-65 -0.09871171360553888 1105 66-67 -0.16427517727932184 1105 68-69 -0.14281546102467502 1105 70-71 -0.16236984324532244 1105 72-73 -0.12262096908774112 1105 74-75 -0.20778936170959383 1105 76-77 -0.17652412005634233 1105 78-79 -0.08580238827229536 1105 80-81 -0.1625550342130353 1105 82-83 -0.16763873591478884 1105 84-85 -0.17352631115652528 1105 86-87 -0.175953681427508 1105 88-89 -0.05906292914578515 1105 90-91 -0.2378628686573201 1105 92-93 -0.07397953451777894 1105 94-95 -0.010474191629498364 1105 96-97 -0.13762314117934338 1105 98-99 -0.06831204687402348 1105 100-101 0.006731320819785225 1106 1 -0.25592549263935993 1106 2 -0.11351349197932592 1106 3 -0.1297371133725136 1106 4 -0.05105508030906947 1106 5 -0.16695922930786367 1106 6 -0.05604644440303019 1106 7 -0.129249143228499 1106 8 -0.10294475063727049 1106 9 -0.08865152632196072 1106 10-11 -0.18870702357190083 1106 12-13 -0.3320945901953536 1106 14-15 -0.19784743424266793 1106 16-17 -0.1357921398096522 1106 18-19 -0.04417254002601112 1106 20-21 -0.34246467009633363 1106 22-23 -0.24693618532238304 1106 24-25 -0.2084021915490979 1106 26-27 -0.1299855011079174 1106 28-29 -0.17428707042579816 1106 30-31 -0.25536250152761397 1106 32-33 -0.19132398603762368 1106 34-35 -0.19968241756100724 1106 36-37 -0.1519121998913704 1106 38-39 -0.0906995135036297 1106 40-41 -0.2198373152743187 1106 42-43 -0.36913670068565096 1106 44-45 -0.3147389710452799 1106 46-47 -0.10468339649672487 1106 48-49 -0.4268697933076524 1106 50-51 -0.19985741208452623 1106 52-53 0.0381274326728942 1106 54-55 -0.02745906417985111 1106 56-57 -0.015994659563546065 1106 58-59 -0.1553727999697614 1106 60-61 4.5361906258989393E-4 1106 62-63 -0.10382101429459567 1106 64-65 -0.09793553346142403 1106 66-67 -0.022886616329422793 1106 68-69 0.04685670069269321 1106 70-71 -0.02610969677835584 1106 72-73 -0.010835922740916715 1106 74-75 -0.10347526899718673 1106 76-77 -0.17826152387863203 1106 78-79 -0.059475876156700025 1106 80-81 -0.09329978951151574 1106 82-83 -0.2012456237534046 1106 84-85 -0.09014043379363912 1106 86-87 0.0433109261382576 1106 88-89 -0.015548919878764167 1106 90-91 0.03811374058985706 1106 92-93 -0.017302627299727646 1106 94-95 -0.11684349164405816 1106 96-97 0.10718737085604246 1106 98-99 -0.2010419117023936 1106 100-101 -0.25121910522308966 1107 1 -0.10743098152969566 1107 2 -0.08000234346457802 1107 3 -0.05286086529547873 1107 4 -0.03929955630755444 1107 5 -0.12095107916618275 1107 6 -0.07695016423288337 1107 7 -0.058312865753151755 1107 8 -0.04854414408983132 1107 9 -0.0946674240151637 1107 10-11 -0.0691306152465998 1107 12-13 -0.15364690939447456 1107 14-15 -0.02656270710615871 1107 16-17 -0.05756400918897242 1107 18-19 -0.10735590082801139 1107 20-21 -0.07725948901633473 1107 22-23 -0.030836205506226122 1107 24-25 -0.03757866376725616 1107 26-27 -0.03417087676229613 1107 28-29 -0.13758623702070594 1107 30-31 -0.1127960761886655 1107 32-33 -0.19076682516210752 1107 34-35 -0.1593016330100383 1107 36-37 -0.19619086943141184 1107 38-39 -0.07141063659162938 1107 40-41 -0.21733783345295166 1107 42-43 -0.23507023853768771 1107 44-45 -0.19849221696239994 1107 46-47 -0.15347334413011993 1107 48-49 -0.28501550703996514 1107 50-51 -0.19976708736921722 1107 52-53 -0.23239671961267305 1107 54-55 -0.37081142320334237 1107 56-57 -0.23066180415888482 1107 58-59 -0.306134121867224 1107 60-61 -0.31295752301397073 1107 62-63 -0.21004831256962575 1107 64-65 -0.30876435304914907 1107 66-67 -0.31331297071796627 1107 68-69 -0.3302777130781678 1107 70-71 -0.3985597893170407 1107 72-73 -0.26427838100750733 1107 74-75 -0.1434260264072762 1107 76-77 -0.21239910485507707 1107 78-79 -0.245231913039607 1107 80-81 -0.25203060365911156 1107 82-83 -0.26948784774620194 1107 84-85 -0.20127664264937195 1107 86-87 -0.27359856686707573 1107 88-89 -0.2393045644943399 1107 90-91 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1.0340133676401067 2316 1 -0.12045429097577198 2316 2 -0.015743053050165656 2316 3 0.014590218137250588 2316 4 -0.04836313319507468 2316 5 0.12841613865266055 2316 6 0.05527342951623382 2316 7 0.0759522657275511 2316 8 0.045889735606884585 2316 9 0.017614315753043286 2316 10-11 0.038480870671520506 2316 12-13 0.13286235408298808 2316 14-15 0.1187302724614554 2316 16-17 -0.06148002826601129 2316 18-19 0.0926909316694875 2316 20-21 0.13813951819143 2316 22-23 0.1516576557032394 2316 24-25 -0.05815566282020512 2316 26-27 -0.03173473625157186 2316 28-29 0.21305094013751358 2316 30-31 0.22384073613920208 2316 32-33 -0.017808173862420063 2316 34-35 -0.014570785185881618 2316 36-37 -4.86557817140465E-4 2316 38-39 -0.6510677055105489 2316 40-41 -0.19434194299278573 2316 42-43 0.13945033090011094 2316 44-45 -0.026145554404017446 2316 46-47 -0.12551632795093326 2316 48-49 -0.16848434428970904 2316 50-51 -0.1961213830103432 2316 52-53 -0.09032695951226088 2316 54-55 -0.15027927181954226 2316 56-57 -0.1302278356174611 2316 58-59 -0.23640899223902778 2316 60-61 0.09603124474788416 2316 62-63 0.19110392754545558 2316 64-65 0.01396401090410393 2316 66-67 0.08144056085875917 2316 68-69 -0.09052037252131839 2316 70-71 0.15151010499557316 2316 72-73 0.018004852107750935 2316 74-75 0.05245077624218908 2316 76-77 0.01108382467595348 2316 78-79 -0.012229841256871765 2316 80-81 -0.13096245410513063 2316 82-83 -0.12325680049405108 2316 84-85 -0.027151668404883367 2316 86-87 -0.0659087274869492 2316 88-89 -0.21019280353572256 2316 90-91 -0.14354205283526866 2316 92-93 -0.040763365959985975 2316 94-95 -0.013349168749293483 2316 96-97 -0.06437386201908524 2316 98-99 0.09679536776241804 2316 100-101 -0.004137229907215101 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 6.0 4 19.0 5 44.0 6 85.0 7 228.0 8 471.0 9 984.0 10 1560.0 11 2651.0 12 3959.0 13 5639.0 14 7223.0 15 10011.0 16 13209.0 17 18244.0 18 16654.0 19 10454.0 20 9164.0 21 9408.0 22 9639.0 23 10397.0 24 12304.0 25 14616.0 26 18392.0 27 22702.0 28 28706.0 29 35748.0 30 44679.0 31 56667.0 32 72237.0 33 95045.0 34 130247.0 35 185821.0 36 288458.0 37 450106.0 38 389345.0 39 42900.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.505708662393225 19.364189360024987 14.590875063828943 22.539226913752845 2 16.033188654926885 20.48667047564492 39.59621196617112 23.883928903257072 3 20.02548018150293 24.121337595556874 29.514196702838746 26.338985520101442 4 13.097070968400512 16.523714358344908 36.60211720387961 33.77709746937497 5 13.690502444998073 37.63389009675422 34.003785323866225 14.671822134381477 6 32.76284551487855 36.19746694687268 16.794819128867182 14.244868409381583 7 29.258354273629006 32.53093721181801 19.90992245442234 18.30078606013065 8 26.989270276738953 35.276919821668464 18.919753285243743 18.814056616348843 9 26.416635159181762 16.610382653655357 19.874442466176323 37.098539720986565 10-11 25.278401458049128 26.922844404890096 26.76628152379612 21.03247261326466 12-13 26.18896059408075 23.871064456621525 27.782343838852867 22.157631110444854 14-15 22.6809293576586 25.391729297600296 26.131779415561347 25.79556192917976 16-17 22.14508738433697 28.91921315693106 26.69475168715768 22.240947771574284 18-19 22.08237799170383 27.16112027567356 29.290241665299828 21.466260067322782 20-21 22.71940730580529 26.188740653674188 29.62938278199777 21.462469258522752 22-23 22.342018635217816 26.123026332416092 29.59132450977623 21.943630522589864 24-25 22.32559879664613 26.30225618940088 29.647678065674558 21.72446694827843 26-27 22.099968929433143 26.5685804715293 29.58237590232246 21.74907469671509 28-29 21.863656658468695 26.821736004190335 29.55305778702355 21.761549550317422 30-31 22.062238356881213 26.40266479552194 29.60474545295974 21.93035139463711 32-33 21.88632132481087 26.516732332724985 29.56939880745615 22.027547535007997 34-35 22.365524154885012 26.557539274513527 29.361201641791858 21.715734928809603 36-37 22.391220753684777 26.39142986830734 29.354143046294606 21.863206331713272 38-39 22.13686119631522 26.668657056634444 29.408383423437712 21.78609832361263 40-41 22.34073392076859 26.57378106854281 29.29838288476233 21.78710212592627 42-43 22.225818026111813 26.597594192440816 29.100538015962062 22.076049765485305 44-45 22.230987607276173 26.831542898824058 28.890769702666795 22.046699791232975 46-47 22.481106658493715 27.040086935881813 28.457527332226977 22.02127907339749 48-49 22.62006463703855 27.116684621399596 28.22996891039713 22.033281831164725 50-51 22.521993510034623 27.307903194116466 28.141584018342563 22.02851927750635 52-53 22.702601989164698 27.455497506730563 27.880168461070753 21.961732043033987 54-55 22.51039809200865 27.34799162652249 27.907198004398825 22.234412277070035 56-57 22.523796616418217 27.52226294932853 27.890615592661984 22.063324841591268 58-59 22.70674649994376 27.27737174554109 27.88818749290772 22.12769426160743 60-61 22.555931773370087 27.297837157755623 28.266004769977194 21.8802262988971 62-63 22.421023577839673 27.345754963110224 28.28832842179958 21.94489303725052 64-65 22.369835270775244 27.295186069248413 28.12772696662453 22.20725169335181 66-67 22.336783382283492 27.435223006096514 27.90860335206604 22.319390259553952 68-69 22.38143068592827 27.337925580229122 28.08013125613529 22.200512477707317 70-71 22.45244021547651 27.302891569488995 27.90540717994053 22.339261035093973 72-73 22.578676626170257 27.257302757776237 27.852410186324445 22.311610429729058 74-75 22.335965756856037 27.625481965412956 27.713661628937796 22.32489064879321 76-77 22.487870650666313 27.421967563560468 27.741386563887023 22.348775221886196 78-79 22.512300307377608 27.36929266480975 27.752040470972066 22.366366556840575 80-81 22.443198570493433 27.601646945376178 27.662126450479896 22.29302803365049 82-83 22.432317053681793 27.53414777427794 27.721037912955232 22.312497259085035 84-85 22.33928317468907 27.46399099719182 27.610891177912002 22.585834650207108 86-87 22.354548152934605 27.44828023640772 27.618569314071728 22.57860229658594 88-89 22.361683793028778 27.371101351361393 27.551375369851623 22.715839485758206 90-91 22.379156051105348 27.30546204390667 27.64740659834053 22.66797530664745 92-93 22.393003310731558 27.114351310194383 27.77196339116831 22.720681987905746 94-95 22.24835595347761 27.10534831090975 27.753254520849204 22.89304121476344 96-97 22.107303178977215 27.195188001817606 27.75506320740085 22.94244561180433 98-99 22.017228431197896 27.260832065783898 27.508329404115905 23.213610098902304 100-101 21.831701213391288 27.487734630405924 27.60387744021941 23.076686715983378 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 239.0 1 182.0 2 178.0 3 263.5 4 233.5 5 222.0 6 442.0 7 936.0 8 1574.0 9 2033.0 10 2048.0 11 1967.0 12 2108.0 13 2318.0 14 2544.5 15 2798.0 16 2999.0 17 3124.0 18 3303.5 19 3563.5 20 3947.5 21 4618.0 22 5450.5 23 6469.5 24 7738.0 25 9380.5 26 11796.0 27 14625.0 28 17204.5 29 20212.5 30 23813.5 31 27611.5 32 31791.0 33 35441.0 34 39270.0 35 42975.5 36 47221.0 37 51589.0 38 54361.5 39 56322.0 40 58733.5 41 62157.5 42 65116.0 43 68278.0 44 71763.0 45 74134.0 46 75006.0 47 77212.0 48 81225.5 49 80444.5 50 76392.0 51 73451.5 52 70378.5 53 68266.0 54 66196.0 55 61523.5 56 55514.5 57 49665.5 58 44863.5 59 40041.5 60 33728.5 61 27573.5 62 22532.0 63 17475.0 64 13046.5 65 10057.0 66 7481.0 67 5929.0 68 4477.5 69 3012.0 70 2169.0 71 1529.0 72 1124.5 73 849.0 74 643.0 75 496.5 76 323.0 77 170.0 78 105.0 79 70.0 80 36.0 81 18.5 82 13.5 83 8.5 84 3.0 85 1.5 86 2.0 87 3.0 88 1.5 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04603478921864246 2 0.0 3 0.0 4 0.0 5 0.012190051827541489 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006516226891551649 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001585697798704584 24-25 6.194132026189782E-4 26-27 0.002576758922894949 28-29 0.002973183372571095 30-31 0.0011397202928189196 32-33 0.0 34-35 0.0 36-37 3.468713934666278E-4 38-39 4.4597750588566427E-4 40-41 4.955305620951825E-5 42-43 0.012636029333427154 44-45 0.0 46-47 0.0 48-49 4.955305620951825E-5 50-51 2.4776528104759126E-5 52-53 2.973183372571095E-4 54-55 2.4776528104759126E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.212009777809051E-4 84-85 9.91061124190365E-5 86-87 0.0 88-89 0.0 90-91 0.008324913443199066 92-93 0.002774971147733022 94-95 0.0 96-97 0.0 98-99 0.0018582396078569343 100-101 0.013106783367417576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2018039.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.863870668818855 #Duplication Level Percentage of deduplicated Percentage of total 1 79.77894091686963 49.354340829765626 2 12.095354128658348 14.965308470177682 3 3.6567952414479987 6.786705236378736 4 1.5304470225301303 3.7871750666913147 5 0.7725235508454276 2.3895648519059116 6 0.491961337357895 1.8260779529020783 7 0.30662613152402335 1.3278355540997695 8 0.22446918278519676 1.1109225994367096 9 0.16368282136898352 0.9113447602690358 >10 0.8872234871201944 9.956574595630467 >50 0.06198426986437729 2.5813167937640094 >100 0.028153414522459265 3.2248857377255336 >500 0.001358887686611228 0.5214567761018029 >1k 3.197382792026376E-4 0.49861059017870263 >5k 1.598691396013188E-4 0.757880184972607 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8533 0.42283622863581927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6793 0.33661391083125747 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4813 0.2384988595364113 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2222 0.11010689089754955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.955305620951825E-5 7 0.0 0.0 0.0 0.0 4.955305620951825E-5 8 0.0 0.0 0.0 9.91061124190365E-5 4.955305620951825E-5 9 0.0 0.0 0.0 9.91061124190365E-5 9.91061124190365E-5 10-11 0.0 0.0 0.0 9.91061124190365E-5 9.91061124190365E-5 12-13 0.0 0.0 0.0 9.91061124190365E-5 9.91061124190365E-5 14-15 0.0 0.0 0.0 9.91061124190365E-5 1.2388264052379561E-4 16-17 2.4776528104759126E-5 0.0 0.0 9.91061124190365E-5 1.4865916862855474E-4 18-19 1.2388264052379564E-4 0.0 0.0 9.91061124190365E-5 1.734356967333139E-4 20-21 1.98212224838073E-4 0.0 0.0 9.91061124190365E-5 1.98212224838073E-4 22-23 1.98212224838073E-4 0.0 0.0 1.2388264052379561E-4 2.725418091523504E-4 24-25 1.98212224838073E-4 0.0 0.0 1.98212224838073E-4 3.468713934666278E-4 26-27 1.98212224838073E-4 0.0 0.0 2.4776528104759123E-4 3.716479215713869E-4 28-29 2.2298875294283213E-4 0.0 0.0 4.955305620951826E-4 3.96424449676146E-4 30-31 2.4776528104759123E-4 0.0 0.0 0.0013379325176569928 3.96424449676146E-4 32-33 2.4776528104759123E-4 0.0 0.0 0.002601535450999708 3.96424449676146E-4 34-35 2.725418091523503E-4 0.0 0.0 0.0038403618562376646 3.96424449676146E-4 36-37 2.973183372571095E-4 0.0 0.0 0.005995919801351708 3.96424449676146E-4 38-39 3.468713934666278E-4 0.0 0.0 0.009464633736017986 3.96424449676146E-4 40-41 3.468713934666278E-4 0.0 0.0 0.014172174075922219 3.96424449676146E-4 42-43 4.212009777809051E-4 0.0 0.0 0.01843373690994079 3.96424449676146E-4 44-45 4.955305620951825E-4 0.0 0.0 0.020514965270740558 4.212009777809051E-4 46-47 5.698601464094598E-4 0.0 0.0 0.02378546698056876 5.203070901999416E-4 48-49 5.94636674514219E-4 0.0 0.0 0.02856733690478727 5.94636674514219E-4 50-51 5.94636674514219E-4 0.0 0.0 0.03401817308783428 5.94636674514219E-4 52-53 5.94636674514219E-4 0.0 0.0 0.04937962051278494 5.94636674514219E-4 54-55 5.94636674514219E-4 0.0 0.0 0.059364561339002864 5.94636674514219E-4 56-57 5.94636674514219E-4 0.0 0.0 0.08689128406339025 5.94636674514219E-4 58-59 5.94636674514219E-4 0.0 0.0 0.13790615543108928 5.94636674514219E-4 60-61 5.94636674514219E-4 0.0 0.0 0.18631949134778863 6.441897307237373E-4 62-63 5.94636674514219E-4 0.0 0.0 0.21126945514928108 6.441897307237373E-4 64-65 5.94636674514219E-4 0.0 0.0 0.2586669534136853 6.441897307237373E-4 66-67 5.94636674514219E-4 0.0 0.0 0.34994368295161793 6.441897307237373E-4 68-69 5.94636674514219E-4 0.0 0.0 0.48343961638006006 6.441897307237373E-4 70-71 5.94636674514219E-4 0.0 0.0 0.6158206060437881 6.441897307237373E-4 72-73 5.94636674514219E-4 0.0 0.0 0.7522897228448013 6.441897307237373E-4 74-75 5.94636674514219E-4 0.0 0.0 0.9128168484355357 6.441897307237373E-4 76-77 6.194132026189781E-4 0.0 0.0 1.0323388200128938 7.432958431427737E-4 78-79 6.441897307237373E-4 0.0 0.0 1.2014386243278747 7.432958431427737E-4 80-81 6.441897307237373E-4 0.0 0.0 1.4245512599112307 7.680723712475329E-4 82-83 6.441897307237373E-4 0.0 0.0 1.624795160053894 7.92848899352292E-4 84-85 6.441897307237373E-4 0.0 0.0 1.8706030953812092 7.92848899352292E-4 86-87 6.441897307237373E-4 0.0 0.0 2.1897495538986114 7.92848899352292E-4 88-89 6.441897307237373E-4 0.0 0.0 2.6228928182260107 7.92848899352292E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6940 0.0 32.869545 1 GTATCAA 10440 0.0 31.864674 1 TATCAAC 12850 0.0 25.65642 2 ATCAACG 12855 0.0 25.609486 3 TCAACGC 12975 0.0 25.372637 4 CAACGCA 13105 0.0 25.120941 5 AACGCAG 13380 0.0 24.671972 6 GTACATG 8335 0.0 23.71918 1 TACATGG 8380 0.0 22.845505 2 GTACGGG 150 3.0229217E-5 22.177814 1 ACATGGG 8425 0.0 21.764923 3 ACGCAGA 15115 0.0 21.748919 7 GAGTACT 8210 0.0 21.640543 12-13 GTCGTAA 110 0.0028697278 21.601767 1 AGTACTT 8570 0.0 21.368956 12-13 CGCAGAG 15330 0.0 21.288952 8 AGAGTAC 12325 0.0 20.967745 10-11 GTACTTT 8505 0.0 20.8589 14-15 GCAGAGT 16115 0.0 19.809736 9 TACTTTT 9625 0.0 18.875822 14-15 >>END_MODULE