FastQCFastQC Report
Thu 26 May 2016
SRR937485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217851
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71240.321211839749379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51970.23432593082222383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37700.1699843677505838No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33290.15010025470601945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97250.034.966421
GGTATCA62000.033.2147451
ATCAACG117750.028.3819643
TCAACGC118050.028.3481854
TATCAAC118650.028.2868772
CAACGCA119850.027.9192715
AACGCAG123800.027.0899416
GTACATG88950.026.1455421
TACATGG89350.025.6980762
ACATGGG90850.024.1749213
ACGCAGA139900.023.735227
CGCAGAG141250.023.4080058
CCGTCTA1852.5421286E-723.1097249
GTACCTG19750.022.8765851
TACCTGG20500.021.5663742
GCAGAGT151100.021.1894369
GGACCGT2509.493306E-920.9014636
AGAGTAC114600.020.80884610-11
CATGGGG40700.020.6727094
ACTATCG1150.003721867720.654558
Produced by FastQC (version 0.11.5)