FastQCFastQC Report
Thu 26 May 2016
SRR937485_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937485_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217851
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91310.4117048440134166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73120.3296885137910527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51680.233018358762604No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24060.10848339225673861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107350.034.7512551
GGTATCA68850.034.580931
ATCAACG133150.027.829133
TCAACGC134150.027.6216814
CAACGCA135600.027.361355
TATCAAC136050.027.3406852
AACGCAG140550.026.392366
GTACATG85800.022.875241
ACGCAGA162050.022.6881437
CGCAGAG163200.022.4991638
TACATGG88100.022.0003932
AGAGTAC137000.021.5143510-11
ACATGGG89100.021.1669833
GCAGAGT171900.021.0564969
AGTACTT96950.020.89672512-13
GAGTACT93150.020.80579612-13
GTACTTT96150.020.0555614-15
TAACGCA1908.248295E-620.0024224
GAGTACA70700.019.0226021
CTAGACT9300.018.9001374
Produced by FastQC (version 0.11.5)