##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937485_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2217851 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08453498454134 31.0 31.0 34.0 30.0 34.0 2 32.31193213610833 34.0 31.0 34.0 31.0 34.0 3 32.396734045704605 34.0 31.0 34.0 31.0 34.0 4 36.01732578067688 37.0 35.0 37.0 35.0 37.0 5 35.898770476465735 37.0 35.0 37.0 35.0 37.0 6 35.97537075303977 37.0 35.0 37.0 35.0 37.0 7 35.9239277120059 37.0 35.0 37.0 35.0 37.0 8 35.901815315816975 37.0 35.0 37.0 35.0 37.0 9 37.58978488636072 39.0 37.0 39.0 35.0 39.0 10-11 37.508685434684296 39.0 37.0 39.0 35.0 39.0 12-13 37.42161308401691 39.0 37.0 39.0 34.0 39.0 14-15 38.626698547377615 40.0 38.0 41.0 34.0 41.0 16-17 38.60680203494283 40.0 38.0 41.0 34.0 41.0 18-19 38.59849714881658 40.0 38.0 41.0 34.0 41.0 20-21 38.48433145418696 40.0 38.0 41.0 34.0 41.0 22-23 38.356037668896604 40.0 38.0 41.0 34.0 41.0 24-25 38.21192045813718 40.0 38.0 41.0 33.5 41.0 26-27 38.15880192131933 40.0 38.0 41.0 33.5 41.0 28-29 38.060366318566935 40.0 37.5 41.0 33.0 41.0 30-31 38.150733074494184 40.0 38.0 41.0 34.0 41.0 32-33 38.09001393691461 40.0 37.0 41.0 33.5 41.0 34-35 37.98030638667791 40.0 37.0 41.0 33.0 41.0 36-37 37.8192268100968 40.0 37.0 41.0 33.0 41.0 38-39 37.69539590351201 40.0 37.0 41.0 33.0 41.0 40-41 37.53058749212639 40.0 37.0 41.0 33.0 41.0 42-43 37.32167986036934 40.0 36.5 41.0 32.0 41.0 44-45 37.04344363079395 40.0 36.0 41.0 31.0 41.0 46-47 36.71823264953326 39.5 36.0 41.0 31.0 41.0 48-49 36.428843055732784 39.0 36.0 41.0 30.0 41.0 50-51 36.20228004496244 39.0 35.0 41.0 30.0 41.0 52-53 35.96643733055106 39.0 35.0 40.5 29.0 41.0 54-55 35.731090817191955 39.0 35.0 40.0 28.5 41.0 56-57 35.467276656547256 38.0 35.0 40.0 28.0 41.0 58-59 35.20983172449367 38.0 34.0 40.0 28.0 41.0 60-61 34.87641076880277 38.0 34.0 40.0 27.0 41.0 62-63 34.49482269097428 37.0 34.0 40.0 26.0 41.0 64-65 34.1398423518983 37.0 33.5 39.5 26.0 41.0 66-67 33.707226274443144 36.0 33.0 39.0 25.5 40.5 68-69 33.29546957843426 36.0 33.0 39.0 25.0 40.0 70-71 32.890301467501644 35.0 32.0 38.5 24.0 40.0 72-73 32.43927657899471 35.0 32.0 37.5 22.5 40.0 74-75 31.990777784440887 35.0 31.0 37.0 22.0 39.0 76-77 30.684030396992405 33.5 29.5 35.5 20.5 38.5 78-79 31.33177521844344 34.0 31.0 36.0 21.0 38.5 80-81 31.190614698642968 34.0 31.0 36.0 20.0 37.5 82-83 30.866746909508347 34.0 31.0 35.0 19.5 37.0 84-85 30.49931036846028 34.0 31.0 35.0 18.0 36.5 86-87 30.124433066062597 34.0 30.0 35.0 16.5 36.0 88-89 29.716963853748517 34.0 30.0 35.0 9.0 36.0 90-91 29.260475793910413 34.0 29.0 35.0 4.5 35.0 92-93 28.87764394452107 34.0 29.0 35.0 2.0 35.0 94-95 28.433412118307317 34.0 29.0 35.0 2.0 35.0 96-97 27.924600660729688 33.0 28.5 35.0 2.0 35.0 98-99 27.399013279070594 33.0 27.0 35.0 2.0 35.0 100-101 25.4845341278562 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17096220731386325 1101 2 0.17589008924113614 1101 3 0.14723079197765543 1101 4 0.06067753421691435 1101 5 0.04721074832667682 1101 6 0.06178242709859205 1101 7 0.04702441731790685 1101 8 0.03824569191078098 1101 9 0.2144288107133221 1101 10-11 0.08452686865367554 1101 12-13 0.037027381765071254 1101 14-15 0.08723105977973944 1101 16-17 0.04452937554896863 1101 18-19 0.09553929327510247 1101 20-21 0.10898498268237233 1101 22-23 0.0029577009459771375 1101 24-25 0.18457403016792995 1101 26-27 0.15135234831407018 1101 28-29 0.06722708696443647 1101 30-31 0.0947110889989844 1101 32-33 0.06842522816645413 1101 34-35 0.034119643091486296 1101 36-37 0.1119813387305939 1101 38-39 -0.09250507815645648 1101 40-41 -0.03695686213220739 1101 42-43 0.13805034483149825 1101 44-45 0.11392568992486218 1101 46-47 0.05669885804630326 1101 48-49 0.06888616253606017 1101 50-51 -0.00123804186028309 1101 52-53 -0.06761969845450011 1101 54-55 -0.044021527618774314 1101 56-57 -0.060525270833423406 1101 58-59 -0.14326989723696215 1101 60-61 -0.08763096082942923 1101 62-63 -0.09893496289987525 1101 64-65 0.0011671881070256518 1101 66-67 0.05054234281627146 1101 68-69 0.008111168925140078 1101 70-71 -0.15318258255092587 1101 72-73 -0.018844426735277864 1101 74-75 0.055114350693116876 1101 76-77 0.057329839161536 1101 78-79 0.045183482794950436 1101 80-81 0.08426753087292482 1101 82-83 0.03537733681611854 1101 84-85 -0.047653252145398284 1101 86-87 -0.061084834700448454 1101 88-89 -0.1960842420155373 1101 90-91 -0.1219034128334151 1101 92-93 -0.1926265320581102 1101 94-95 -0.18783112241532507 1101 96-97 -0.17092286981090865 1101 98-99 -0.001263791240749157 1101 100-101 0.4936639677312833 1102 1 0.0334950292339542 1102 2 0.11156054697872975 1102 3 0.047276883593383445 1102 4 0.028560962694143655 1102 5 -0.08858993125227954 1102 6 -0.015789335841759566 1102 7 -0.046107410861267795 1102 8 0.01117693392586716 1102 9 0.09013587646801113 1102 10-11 0.0011601390314552873 1102 12-13 0.02331796832233124 1102 14-15 -0.006884774656143122 1102 16-17 0.0034700594765340043 1102 18-19 0.10019440435210925 1102 20-21 0.030192952505395 1102 22-23 0.1055868652381946 1102 24-25 0.11895825707700425 1102 26-27 0.0871034545365248 1102 28-29 0.11263230237523203 1102 30-31 0.20637063349254703 1102 32-33 0.18659598315470305 1102 34-35 0.2250934788755501 1102 36-37 0.2388094302677004 1102 38-39 0.061740300720892094 1102 40-41 0.15565031271702168 1102 42-43 0.258226890404643 1102 44-45 0.29001603138652143 1102 46-47 0.2772555291800174 1102 48-49 0.3327829267909479 1102 50-51 0.33845870562540625 1102 52-53 0.33658719179203445 1102 54-55 0.3099788977694189 1102 56-57 0.16580860299871603 1102 58-59 0.18166663797148885 1102 60-61 0.15578523319684479 1102 62-63 0.10959478087715979 1102 64-65 0.1047625946725006 1102 66-67 0.2725260539540031 1102 68-69 0.363154340141989 1102 70-71 0.32366561781543624 1102 72-73 0.37668511287437667 1102 74-75 0.2383001008519301 1102 76-77 0.17525249051422875 1102 78-79 0.26233681155228084 1102 80-81 0.28381592304294045 1102 82-83 0.29237409619183197 1102 84-85 0.24683084151011414 1102 86-87 0.3201313160290873 1102 88-89 0.35092873232427024 1102 90-91 0.27716044892395075 1102 92-93 0.2947748955977083 1102 94-95 0.23207818179076156 1102 96-97 0.011667431622573332 1102 98-99 0.12575200638816142 1102 100-101 0.5947506261270412 1103 1 1.18992664290829E-4 1103 2 -0.006216754242146294 1103 3 0.021492819002169483 1103 4 0.04412264807968569 1103 5 -0.12600945971323796 1103 6 -0.14662733312538023 1103 7 -0.1252679512020478 1103 8 -0.08390738160525757 1103 9 -0.12789848515365065 1103 10-11 -0.17357505651806804 1103 12-13 -0.16340917434992974 1103 14-15 -0.17092909351383412 1103 16-17 -0.18360340457355306 1103 18-19 -0.12614921747102414 1103 20-21 -0.14588034007765316 1103 22-23 -0.22637893666457387 1103 24-25 -0.17705249442052917 1103 26-27 -0.1975672282900902 1103 28-29 -0.34087560029315966 1103 30-31 -0.24411954266063418 1103 32-33 -0.28222761743828784 1103 34-35 -0.2616021736640164 1103 36-37 -0.26361505298343246 1103 38-39 -0.32072990396029866 1103 40-41 -0.29481218108657004 1103 42-43 -0.15114039620649322 1103 44-45 -0.28131834663430055 1103 46-47 -0.34229916576983044 1103 48-49 -0.25401770843137683 1103 50-51 -0.23183378818286116 1103 52-53 -0.08437701364510986 1103 54-55 -0.16758041137257607 1103 56-57 -0.29053516366032994 1103 58-59 -0.2671894619335262 1103 60-61 -0.2758755722871058 1103 62-63 -0.34173182272513714 1103 64-65 -0.24130590430874577 1103 66-67 -0.16773333316577776 1103 68-69 -0.20338480083148625 1103 70-71 -0.33335182276562136 1103 72-73 -0.1750953644581017 1103 74-75 -0.3311661273823887 1103 76-77 -0.2964386277265483 1103 78-79 -0.20456490979928788 1103 80-81 -0.22179460008104712 1103 82-83 -0.367827281805166 1103 84-85 -0.3367415878975599 1103 86-87 -0.35931057977034797 1103 88-89 -0.36833136168825575 1103 90-91 -0.33375268082614085 1103 92-93 -0.23787871340977418 1103 94-95 -0.1916219041296472 1103 96-97 -0.2295197697409428 1103 98-99 -0.22461531619834574 1103 100-101 -0.333397557840744 1104 1 -0.030668458916117913 1104 2 -0.012442327110150586 1104 3 -0.018545257812149885 1104 4 -0.06041930001469353 1104 5 -0.029056565701480963 1104 6 -0.05148928751556525 1104 7 -0.11496154311486606 1104 8 -0.027994585837355146 1104 9 -0.024602429457729613 1104 10-11 -0.11932566207759265 1104 12-13 -0.02829401966802436 1104 14-15 -0.10469765035589518 1104 16-17 -0.14131501182507833 1104 18-19 -0.13102675123548124 1104 20-21 -0.29502242214091723 1104 22-23 -0.10381890459989052 1104 24-25 -0.11779322160440842 1104 26-27 -0.35660312810991 1104 28-29 -0.22175446917110264 1104 30-31 -0.21692541313357339 1104 32-33 -0.25262108700382413 1104 34-35 -0.1530559145897712 1104 36-37 -0.17295567712839244 1104 38-39 -0.27767100706235937 1104 40-41 -0.11886419560718764 1104 42-43 -0.20148831268824097 1104 44-45 -0.14901663020362577 1104 46-47 -0.1437149900216781 1104 48-49 -0.10247191879730622 1104 50-51 -0.22733658789478994 1104 52-53 -0.1963074971501868 1104 54-55 -0.06501859946160948 1104 56-57 -0.1167162449466943 1104 58-59 -0.2281453995281737 1104 60-61 -0.11314946546522009 1104 62-63 -0.08718417820756486 1104 64-65 -0.22129029748182205 1104 66-67 -0.05374298642976072 1104 68-69 0.012457478250247789 1104 70-71 -0.14479300868315192 1104 72-73 -0.09999385831465446 1104 74-75 -0.23627950221801797 1104 76-77 -0.2772994464924423 1104 78-79 -0.07986963412443515 1104 80-81 -0.09575197161869653 1104 82-83 -0.03320600015112163 1104 84-85 -0.07481490200794028 1104 86-87 -0.1352277039047891 1104 88-89 -0.034508951913650776 1104 90-91 -0.026557846681278363 1104 92-93 -0.2526516663349021 1104 94-95 -0.17823713979684896 1104 96-97 -0.09364749407249207 1104 98-99 -0.023112009527629596 1104 100-101 0.3407005771182732 1105 1 -0.02716325583795509 1105 2 0.06070697609067821 1105 3 -0.040726733336406085 1105 4 -0.0022961500517126865 1105 5 0.026080722891805408 1105 6 0.0629409658826603 1105 7 -0.010302524878049724 1105 8 0.02981752462690679 1105 9 0.010722467793200963 1105 10-11 -0.03315876676547447 1105 12-13 -0.0873388008729421 1105 14-15 -0.07342893202242351 1105 16-17 -0.023277583876669894 1105 18-19 -0.0015978551005275676 1105 20-21 -0.001089447853985348 1105 22-23 -0.035171544689013956 1105 24-25 -0.10039173983108896 1105 26-27 -0.13150478543203548 1105 28-29 -0.14599243418104635 1105 30-31 -0.04123710864287489 1105 32-33 0.0657268973895313 1105 34-35 0.003178341981055155 1105 36-37 -0.09681179402646478 1105 38-39 -0.014577952527240257 1105 40-41 0.02926253366639031 1105 42-43 -0.06777266782559366 1105 44-45 -0.13905706298209708 1105 46-47 -0.18906596275058263 1105 48-49 -0.1410458239029282 1105 50-51 -0.08454613002108857 1105 52-53 -0.11597477207187978 1105 54-55 -0.15358279636469518 1105 56-57 -0.11424395220385719 1105 58-59 -0.11799299959924525 1105 60-61 -0.08466678918711779 1105 62-63 -0.1036635574669873 1105 64-65 -0.2030850024585007 1105 66-67 -0.1512598190862704 1105 68-69 -0.1010351795681288 1105 70-71 -0.09738463927001817 1105 72-73 -0.06537363047350908 1105 74-75 -0.04791256797883392 1105 76-77 -0.1111678895088275 1105 78-79 -0.06958477142716646 1105 80-81 -0.17246986397735142 1105 82-83 -0.07678469573629698 1105 84-85 -0.04074635691404538 1105 86-87 0.012269582746856855 1105 88-89 0.10922611171042007 1105 90-91 0.07140251180588209 1105 92-93 -0.058210154249369594 1105 94-95 -0.09671510099891378 1105 96-97 -0.060555247768796505 1105 98-99 0.14774599292958257 1105 100-101 0.6362376433654475 1106 1 -0.1849818489917716 1106 2 -0.16698866667401546 1106 3 -0.17892576515527736 1106 4 -0.05912938568447146 1106 5 -0.196495094851457 1106 6 -0.2025726764786242 1106 7 -0.2025818424028003 1106 8 -0.21664542584625934 1106 9 -0.3858027799250152 1106 10-11 -0.4715383345297468 1106 12-13 -0.46770629158612564 1106 14-15 -0.4737887301289305 1106 16-17 -0.3940738986742076 1106 18-19 -0.39680125613089956 1106 20-21 -0.2963123364711393 1106 22-23 -0.1672541794107545 1106 24-25 -0.12022528379844744 1106 26-27 -0.1000614197209444 1106 28-29 -0.20471960323875038 1106 30-31 -0.248392716760641 1106 32-33 -0.26873110869941996 1106 34-35 -0.4302675439845203 1106 36-37 -0.19774567490321004 1106 38-39 -0.17000314435652797 1106 40-41 -0.30248749890046867 1106 42-43 -0.6061076662707876 1106 44-45 -0.43605386039943284 1106 46-47 -0.2716355507693393 1106 48-49 -0.2037667007287709 1106 50-51 -0.24243136704476598 1106 52-53 -0.15569084160947 1106 54-55 -0.35035043079226824 1106 56-57 -0.2725594525568411 1106 58-59 -0.35050124899413504 1106 60-61 -0.24470334285729933 1106 62-63 -0.3565016014385378 1106 64-65 -0.5081154393376295 1106 66-67 -0.42884381531272453 1106 68-69 -0.2804156581538493 1106 70-71 -0.490799489546518 1106 72-73 -0.3602818812341084 1106 74-75 -0.5258322392006605 1106 76-77 -0.36127773699088195 1106 78-79 -0.28809866171678067 1106 80-81 -0.31275658888616675 1106 82-83 -0.46522994874925416 1106 84-85 -0.7091919881227611 1106 86-87 -0.596452044692505 1106 88-89 -0.5636693602358598 1106 90-91 -0.6827435248076661 1106 92-93 -0.5907969711858136 1106 94-95 -1.0681632913322296 1106 96-97 -0.9251798149447659 1106 98-99 -0.7847872988022182 1106 100-101 -0.37771390608154576 1107 1 -0.022501224951135157 1107 2 -0.0447603181632914 1107 3 -0.11530363031800306 1107 4 -0.06799018167799176 1107 5 -0.1912678975799409 1107 6 -0.13693775943683306 1107 7 -0.1169229897183044 1107 8 -0.11404702615497797 1107 9 -0.08425289181511886 1107 10-11 -0.1463129913138701 1107 12-13 -0.17983477979778684 1107 14-15 -0.27013560723091246 1107 16-17 -0.2332151017191677 1107 18-19 -0.21753307452830484 1107 20-21 -0.13284270309986113 1107 22-23 -0.12433400405158324 1107 24-25 -0.11622309719852808 1107 26-27 -0.324007693608074 1107 28-29 -0.26447604554312676 1107 30-31 -0.2220463301210529 1107 32-33 -0.31649884457410593 1107 34-35 -0.1495846074406515 1107 36-37 -0.287232155388196 1107 38-39 -0.29544038349521173 1107 40-41 -0.1640996272269888 1107 42-43 -0.14668386256785482 1107 44-45 -0.08829102914868514 1107 46-47 -0.10257478086196414 1107 48-49 -0.06784582794286109 1107 50-51 -0.13623649016700057 1107 52-53 -0.06934871013536537 1107 54-55 -0.0833936367562984 1107 56-57 -0.021221056142501027 1107 58-59 0.06110698527658087 1107 60-61 0.09246085209449717 1107 62-63 -0.14639236306734915 1107 64-65 -0.13649336432599313 1107 66-67 -0.09477977385947867 1107 68-69 -0.18737965821421199 1107 70-71 -0.23351706620957202 1107 72-73 -0.09281212282186146 1107 74-75 -0.15186706095261115 1107 76-77 -0.22250376092544855 1107 78-79 -0.2203579902036985 1107 80-81 -0.18099009425861468 1107 82-83 0.07725958984959647 1107 84-85 0.11260564278218865 1107 86-87 0.05865944926672384 1107 88-89 0.06539891751891957 1107 90-91 -0.12398498483336695 1107 92-93 -0.009836182306695918 1107 94-95 -0.11881902877208006 1107 96-97 -0.11107287689609535 1107 98-99 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-0.3965896023449105 2316 46-47 -0.3649374626121471 2316 48-49 -0.17431181933383755 2316 50-51 -0.1984765465013112 2316 52-53 -0.3124636111261978 2316 54-55 -0.4118242535927692 2316 56-57 -0.38510011240814634 2316 58-59 -0.35992147270629005 2316 60-61 -0.5603634707560374 2316 62-63 -0.31333577562866566 2316 64-65 -0.5162411861512268 2316 66-67 -0.3698801159854028 2316 68-69 -0.12525960177218565 2316 70-71 -0.22069222498642205 2316 72-73 -0.25117932989758884 2316 74-75 -0.02530117175509261 2316 76-77 -0.08981030359559483 2316 78-79 -0.17963090193628872 2316 80-81 -0.23455414155035115 2316 82-83 -0.21557431279599015 2316 84-85 -0.3887384233325335 2316 86-87 -0.46019382974295553 2316 88-89 -0.3864186853376488 2316 90-91 -0.27886327498469043 2316 92-93 0.0028811134309343345 2316 94-95 0.06002726230558153 2316 96-97 -0.2003529650598317 2316 98-99 -0.5569787264885306 2316 100-101 -0.7337828729340892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 18.0 5 50.0 6 103.0 7 225.0 8 579.0 9 1117.0 10 1803.0 11 2957.0 12 4387.0 13 6389.0 14 8021.0 15 11157.0 16 15015.0 17 20038.0 18 18391.0 19 11717.0 20 10570.0 21 10632.0 22 11206.0 23 12003.0 24 14486.0 25 16994.0 26 21001.0 27 26463.0 28 32942.0 29 41191.0 30 51872.0 31 65260.0 32 82961.0 33 108729.0 34 148187.0 35 208812.0 36 319255.0 37 485654.0 38 405805.0 39 41845.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40569683840311 19.380971049334732 14.580086103563378 22.63324600869878 2 15.955219714940272 20.55850460648619 39.54458617824191 23.941689500331627 3 20.000216425720215 24.166005741594002 29.47416215065845 26.359615682027332 4 13.089833356704306 16.58276412617439 36.49316387800623 33.83423863911507 5 13.707869222096955 37.64482525366404 33.9576099210823 14.689695603156707 6 32.809778474748754 36.22736604036971 16.760684103666115 14.20217138121542 7 29.27279605347699 32.498215615025536 19.891958476922028 18.337029854575444 8 26.908254882767146 35.36378232802835 18.87845486464149 18.84950792456301 9 26.39198936267585 16.604136166045418 19.898270893761573 37.10560357751716 10-11 25.30541952547759 26.911163103382506 26.798576640180066 20.984840730959835 12-13 26.128888623356595 23.892450403389894 27.770922786090065 22.207738187163443 14-15 22.63051034537487 25.390524431082163 26.15786633096633 25.821098892576643 16-17 22.148940573555212 28.90439889785202 26.6885376880593 22.25812284053347 18-19 22.058763190133153 27.228632581719868 29.25778151913722 21.454822709009758 20-21 22.760275600119215 26.18550569898519 29.60043303179519 21.4537856691004 22-23 22.374185042544955 26.16820807541424 29.5213272610328 21.936279621008005 24-25 22.312284602331406 26.26143254793898 29.649021990034925 21.777260859694692 26-27 22.139511038858124 26.52603936875979 29.577498129360553 21.756951463021533 28-29 21.89508769145306 26.832579124311923 29.540043719473925 21.732289464761095 30-31 22.07269559587186 26.41980908546156 29.551466712597012 21.95602860606957 32-33 21.89644389997344 26.539497017608486 29.516387710445834 22.04767137197224 34-35 22.410477529825044 26.54822167945457 29.31944030505205 21.721860485668333 36-37 22.34879619956435 26.394446696374104 29.350100615415553 21.906656488645993 38-39 22.09894219650964 26.72989004443717 29.356712727030065 21.814455032023123 40-41 22.37745006314671 26.581790210433432 29.240557638903603 21.800202087516247 42-43 22.185659971671146 26.629258629094483 29.03473103145982 22.15035036777455 44-45 22.230145307326776 26.860460869553453 28.85885931922388 22.05053450389589 46-47 22.4409574854217 27.07925374608123 28.460140018423242 22.019648750073834 48-49 22.569415168106424 27.12535693335576 28.210686831531966 22.094541067005853 50-51 22.49639403188041 27.328143324326113 28.179823622055768 21.99563902173771 52-53 22.694739186717232 27.41665242615487 27.88099831774091 22.00761006938699 54-55 22.497611426556606 27.30242022570497 27.939591072619397 22.260377275119023 56-57 22.514474597256534 27.521618900458144 27.901784204619695 22.062122297665624 58-59 22.696001670085142 27.231878967523066 27.94716597282685 22.124953389564944 60-61 22.586503782264906 27.230413585042456 28.26837781257623 21.91470482011641 62-63 22.395688438943825 27.30832684431912 28.30120238014186 21.9947823365952 64-65 22.31805074327598 27.338023910989488 28.14012937301229 22.203795972722236 66-67 22.3549057172912 27.46289087950453 27.907578101504566 22.274625301699707 68-69 22.381169880212873 27.345164305447028 28.040995540277503 22.232670274062592 70-71 22.42571750762337 27.34232371786923 27.882869498446922 22.349089276060475 72-73 22.49722084388503 27.284786456429977 27.846095959171258 22.371896740513726 74-75 22.277488433623358 27.65034711529314 27.660401893544694 22.411762557538808 76-77 22.45563385457364 27.422739399535857 27.73301723154531 22.38860951434519 78-79 22.495469713700334 27.399856888492508 27.705828750443562 22.398844647363596 80-81 22.434239270356755 27.57498136709815 27.65138415520249 22.3393952073426 82-83 22.439475499038142 27.576700138400334 27.65923534211079 22.32458902045074 84-85 22.330323627878666 27.44560549186703 27.627608129586417 22.596462750667882 86-87 22.376165035432948 27.439377126777227 27.619754437967202 22.564703399822623 88-89 22.396139325860933 27.396204704463916 27.50822755901997 22.69942841065518 90-91 22.381040957151182 27.344780277076016 27.657750586495222 22.61642817927758 92-93 22.42183685036132 27.13457620041942 27.724905752427258 22.718681196791998 94-95 22.262271000170884 27.116835170622373 27.686147536511697 22.934746292695046 96-97 22.15996475867856 27.160210492048382 27.758514886707896 22.92130986256516 98-99 22.06496722150794 27.235196924577345 27.50264164504091 23.19719420887381 100-101 21.899774638931884 27.341652565159112 27.694496522630242 23.06407627327876 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 288.0 1 223.0 2 215.5 3 292.0 4 277.0 5 272.5 6 476.5 7 1032.5 8 1735.5 9 2166.0 10 2128.0 11 2019.0 12 2221.0 13 2468.5 14 2709.5 15 2950.0 16 3155.0 17 3386.0 18 3653.5 19 3977.5 20 4367.0 21 5007.0 22 5864.5 23 7033.5 24 8494.0 25 10266.5 26 12947.0 27 16240.5 28 19003.5 29 21968.5 30 26155.0 31 30352.0 32 34921.5 33 38930.5 34 42946.5 35 47002.0 36 51655.5 37 56723.5 38 60096.5 39 62479.0 40 64641.0 41 68237.5 42 71587.5 43 75221.0 44 79496.5 45 82168.0 46 82657.5 47 84928.0 48 89369.5 49 88455.5 50 84083.5 51 80574.0 52 77182.5 53 74678.5 54 72499.0 55 67392.0 56 60448.0 57 54472.0 58 49474.0 59 43932.0 60 36996.5 61 30432.0 62 24848.5 63 19180.0 64 14441.5 65 11184.0 66 8196.5 67 6415.0 68 4910.5 69 3330.5 70 2394.5 71 1685.0 72 1248.5 73 982.0 74 738.0 75 544.0 76 360.5 77 211.0 78 113.5 79 66.5 80 42.0 81 31.0 82 20.0 83 10.0 84 5.5 85 4.0 86 3.5 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05491802650403477 2 0.0 3 0.0 4 0.0 5 0.015826130790571595 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0037649057578710205 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.243181800761187E-4 24-25 2.47987804410666E-4 26-27 0.0011948503303422999 28-29 0.0033816518783272636 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 2.254434585551509E-5 40-41 0.0 42-43 0.014608736114373778 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.254434585551509E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.763303756654527E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.283425712547867E-4 84-85 0.0011723059844867846 86-87 0.0 88-89 0.0 90-91 0.010302766055970396 92-93 9.91951217642664E-4 94-95 0.0 96-97 0.0 98-99 4.283425712547867E-4 100-101 0.01345897447574251 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2217851.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.81389171994811 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9096165766874 47.988008781572894 2 12.640883777773812 15.374826746119702 3 3.809680674130017 6.950445240123653 4 1.5609029770295275 3.796983385216733 5 0.8106877858280006 2.465053961301426 6 0.5097170400552079 1.859872612903792 7 0.32984318075238384 1.404133323518712 8 0.2325849252839525 1.1315515569528405 9 0.17280560759592034 0.9458083358044328 >10 0.9255221397754865 10.169174837247184 >50 0.06676891288133183 2.787407617631869 >100 0.028696029285210374 3.198963636913165 >500 0.0017731919394592592 0.6550958183250956 >1k 2.955319899097613E-4 0.30166758053725845 >5k 2.2164899243232096E-4 0.9710065658312024 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9131 0.4117048440134166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7312 0.3296885137910527 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5168 0.233018358762604 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2406 0.10848339225673861 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.508869171103018E-5 4.508869171103018E-5 9 0.0 0.0 0.0 4.508869171103018E-5 4.508869171103018E-5 10-11 4.508869171103018E-5 0.0 0.0 6.763303756654527E-5 4.508869171103018E-5 12-13 4.508869171103018E-5 0.0 0.0 9.017738342206036E-5 4.508869171103018E-5 14-15 9.017738342206036E-5 0.0 0.0 9.017738342206036E-5 6.763303756654527E-5 16-17 9.017738342206036E-5 0.0 0.0 9.017738342206036E-5 9.017738342206036E-5 18-19 1.3526607513309055E-4 0.0 0.0 9.017738342206036E-5 1.8035476684412073E-4 20-21 1.3526607513309055E-4 0.0 0.0 1.1272172927757546E-4 1.8035476684412073E-4 22-23 1.3526607513309055E-4 0.0 0.0 2.0289911269963582E-4 1.8035476684412073E-4 24-25 1.3526607513309055E-4 0.0 0.0 3.156208419772113E-4 1.8035476684412073E-4 26-27 1.3526607513309055E-4 0.0 0.0 3.6070953368824146E-4 1.8035476684412073E-4 28-29 1.3526607513309055E-4 0.0 0.0 8.341407966540584E-4 1.8035476684412073E-4 30-31 1.3526607513309055E-4 0.0 0.0 0.0021417128562739333 1.8035476684412073E-4 32-33 1.3526607513309055E-4 0.0 0.0 0.003697272720304475 1.8035476684412073E-4 34-35 1.3526607513309055E-4 0.0 0.0 0.0048244900130802295 1.8035476684412073E-4 36-37 1.3526607513309055E-4 0.0 0.0 0.006425138568821801 1.8035476684412073E-4 38-39 1.8035476684412073E-4 0.0 0.0 0.00978424610129355 1.8035476684412073E-4 40-41 1.8035476684412073E-4 0.0 0.0 0.014180393543118992 1.8035476684412073E-4 42-43 1.8035476684412073E-4 0.0 0.0 0.01859908533079995 2.0289911269963582E-4 44-45 1.8035476684412073E-4 0.0 0.0 0.021056419029051095 2.254434585551509E-4 46-47 1.8035476684412073E-4 0.0 0.0 0.024595881328366966 2.705321502661811E-4 48-49 1.8035476684412073E-4 0.0 0.0 0.029375282649736163 3.6070953368824146E-4 50-51 1.8035476684412073E-4 0.0 0.0 0.03550734472243627 3.6070953368824146E-4 52-53 1.8035476684412073E-4 0.0 0.0 0.053182111873160104 3.832538795437566E-4 54-55 1.8035476684412073E-4 0.0 0.0 0.06377795442525219 4.0579822539927164E-4 56-57 1.8035476684412073E-4 0.0 0.0 0.09270235015787806 4.508869171103018E-4 58-59 1.8035476684412073E-4 0.0 0.0 0.14556884118906094 4.95975608821332E-4 60-61 1.8035476684412073E-4 0.0 0.0 0.1963161637098254 4.95975608821332E-4 62-63 1.8035476684412073E-4 0.0 0.0 0.222557782285645 4.95975608821332E-4 64-65 1.8035476684412073E-4 0.0 0.0 0.2728091291975881 5.185199546768471E-4 66-67 1.8035476684412073E-4 0.0 0.0 0.36158876317660654 5.410643005323622E-4 68-69 1.8035476684412073E-4 0.0 0.0 0.4884683416514455 5.410643005323622E-4 70-71 2.0289911269963582E-4 0.0 0.0 0.6095314788955615 5.410643005323622E-4 72-73 2.254434585551509E-4 0.0 0.0 0.7415286238796024 5.410643005323622E-4 74-75 2.254434585551509E-4 0.0 0.0 0.8979863841168771 5.410643005323622E-4 76-77 2.254434585551509E-4 0.0 0.0 1.0155100590616772 6.312416839544226E-4 78-79 2.254434585551509E-4 0.0 0.0 1.1756200033275455 6.312416839544226E-4 80-81 3.832538795437566E-4 0.0 0.0 1.3874241326401098 6.537860298099377E-4 82-83 4.508869171103018E-4 0.0 0.0 1.5854762109808098 6.763303756654527E-4 84-85 4.734312629658169E-4 0.0 0.0 1.832765140669955 6.763303756654527E-4 86-87 4.95975608821332E-4 0.0 0.0 2.1373843418696747 6.763303756654527E-4 88-89 4.95975608821332E-4 0.0 0.0 2.539102040669098 6.763303756654527E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10735 0.0 34.751255 1 GGTATCA 6885 0.0 34.58093 1 ATCAACG 13315 0.0 27.82913 3 TCAACGC 13415 0.0 27.621681 4 CAACGCA 13560 0.0 27.36135 5 TATCAAC 13605 0.0 27.340685 2 AACGCAG 14055 0.0 26.39236 6 GTACATG 8580 0.0 22.87524 1 ACGCAGA 16205 0.0 22.688143 7 CGCAGAG 16320 0.0 22.499163 8 TACATGG 8810 0.0 22.000393 2 AGAGTAC 13700 0.0 21.51435 10-11 ACATGGG 8910 0.0 21.166983 3 GCAGAGT 17190 0.0 21.056496 9 AGTACTT 9695 0.0 20.896725 12-13 GAGTACT 9315 0.0 20.805796 12-13 GTACTTT 9615 0.0 20.05556 14-15 TAACGCA 190 8.248295E-6 20.002422 4 GAGTACA 7070 0.0 19.022602 1 CTAGACT 930 0.0 18.900137 4 >>END_MODULE