FastQCFastQC Report
Thu 26 May 2016
SRR937484_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937484_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474067
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77700.3140577842071375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57420.23208748995075718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41110.16616364876133105No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37460.15141061256627247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100350.038.9252361
GGTATCA61700.034.5040131
ATCAACG124100.031.2008063
TCAACGC124400.031.1268234
TATCAAC126150.030.7684752
CAACGCA126000.030.6573895
AACGCAG128600.030.1096296
GTACATG100900.026.703531
ACGCAGA147900.025.7292237
TACATGG101000.025.5887282
CGCAGAG149050.025.4993538
GTACCTG22100.025.1576671
TACCTGG21450.024.5848082
ACATGGG103550.024.3167613
GCAGAGT158200.023.213699
GAGTACT78800.022.06410812-13
CATGGGA57700.021.7383064
AGAGTAC129000.021.17475910-11
GGACCGA3900.020.7096656
GTATAGG7200.020.4600161
Produced by FastQC (version 0.11.5)