FastQCFastQC Report
Thu 26 May 2016
SRR937484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474067
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98540.39829155798933497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78670.31797845410007086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55500.22432698871938392No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25520.10314999553366985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75750.034.8225021
GTATCAA116000.032.7369651
ATCAACG142950.026.249463
TCAACGC144800.025.9468924
TATCAAC146500.025.6782262
CAACGCA146850.025.6175425
GTACATG100650.025.4521581
AACGCAG151250.024.9660136
TACATGG101300.024.5227782
ACATGGG102750.023.021043
ACGCAGA173200.021.6684027
CGCAGAG174600.021.3858248
GAGTACT96150.021.19565812-13
AGAGTAC145500.020.5369210-11
AGTACTT99400.020.33537112-13
GTACTTT101250.019.8667614-15
GTACCTG23250.019.6244681
GCAGAGT187750.019.5843289
TACCTGG22050.019.3870332
CATGGGA60950.019.32664
Produced by FastQC (version 0.11.5)