##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937484_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2474067 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14000793026219 33.0 31.0 34.0 31.0 34.0 2 32.35159031667291 34.0 31.0 34.0 31.0 34.0 3 32.40954145542542 34.0 31.0 34.0 31.0 34.0 4 36.055723228190665 37.0 35.0 37.0 35.0 37.0 5 35.96643825733094 37.0 35.0 37.0 35.0 37.0 6 36.00214747620012 37.0 35.0 37.0 35.0 37.0 7 35.940705324471814 37.0 35.0 37.0 35.0 37.0 8 35.913990607368355 37.0 35.0 37.0 35.0 37.0 9 37.614248118583696 39.0 37.0 39.0 35.0 39.0 10-11 37.532793372208594 39.0 37.0 39.0 35.0 39.0 12-13 37.42694619830425 39.0 37.0 39.0 34.0 39.0 14-15 38.6265751897584 40.0 38.0 41.0 34.0 41.0 16-17 38.59664208770417 40.0 38.0 41.0 34.0 41.0 18-19 38.57929777164483 40.0 38.0 41.0 34.0 41.0 20-21 38.47629207293093 40.0 38.0 41.0 34.0 41.0 22-23 38.32383197383094 40.0 38.0 41.0 34.0 41.0 24-25 38.266174481127635 40.0 38.0 41.0 33.5 41.0 26-27 38.21065597657622 40.0 38.0 41.0 33.5 41.0 28-29 38.086517665043026 40.0 38.0 41.0 33.0 41.0 30-31 38.160814157417725 40.0 38.0 41.0 34.0 41.0 32-33 38.115768893890106 40.0 37.0 41.0 33.5 41.0 34-35 37.99853237604317 40.0 37.0 41.0 33.0 41.0 36-37 37.83316013673033 40.0 37.0 41.0 33.0 41.0 38-39 37.71263631906493 40.0 37.0 41.0 33.0 41.0 40-41 37.539394446472144 40.0 37.0 41.0 33.0 41.0 42-43 37.32895936124608 40.0 37.0 41.0 32.0 41.0 44-45 37.058810856779544 40.0 36.0 41.0 31.0 41.0 46-47 36.73492815675566 39.5 36.0 41.0 31.0 41.0 48-49 36.42078832141571 39.0 36.0 41.0 30.0 41.0 50-51 36.18253830635953 39.0 35.0 41.0 30.0 41.0 52-53 35.93783373691981 39.0 35.0 40.0 29.0 41.0 54-55 35.70232374466819 39.0 35.0 40.0 28.0 41.0 56-57 35.422402869445335 38.0 35.0 40.0 28.0 41.0 58-59 35.14649118233257 38.0 34.0 40.0 27.5 41.0 60-61 34.817743213906496 38.0 34.0 40.0 26.5 41.0 62-63 34.44270749337023 37.0 34.0 40.0 26.0 41.0 64-65 34.04108983305626 37.0 33.0 39.5 26.0 41.0 66-67 33.616915790881976 36.0 33.0 39.0 25.0 40.5 68-69 33.1779701196451 36.0 32.0 39.0 24.0 40.0 70-71 32.76958566603087 35.0 32.0 38.0 23.5 40.0 72-73 32.299241087650415 35.0 31.5 37.5 22.0 39.5 74-75 31.825094469955744 35.0 31.0 37.0 21.0 39.0 76-77 30.578136121616755 33.5 29.5 35.5 20.0 38.0 78-79 31.182113701852053 34.0 31.0 36.0 20.0 38.5 80-81 30.99824216563254 34.0 31.0 36.0 20.0 37.5 82-83 30.655173445181557 34.0 31.0 35.0 18.0 37.0 84-85 30.291501200250437 34.0 30.5 35.0 17.5 36.5 86-87 29.87731718664046 34.0 30.0 35.0 11.5 36.0 88-89 29.409937160149667 34.0 29.5 35.0 7.0 36.0 90-91 28.890285307552304 34.0 29.0 35.0 2.0 35.0 92-93 28.526029004064966 34.0 29.0 35.0 2.0 35.0 94-95 28.076821282527916 33.0 28.5 35.0 2.0 35.0 96-97 27.58283405421114 33.0 27.0 35.0 2.0 35.0 98-99 26.821545859509868 33.0 26.0 35.0 2.0 35.0 100-101 24.356953348474395 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09744773987243605 1101 2 -0.08166187890525833 1101 3 -0.05491748223442272 1101 4 -0.0547818352885443 1101 5 -0.053477228792033316 1101 6 -0.14979996394420425 1101 7 -0.11524772007280149 1101 8 -0.12927565127540674 1101 9 -0.06954791322098686 1101 10-11 -0.04364120858847542 1101 12-13 -0.12608136097292544 1101 14-15 -0.2590565932544351 1101 16-17 -0.15596783253909052 1101 18-19 -0.12435201578936272 1101 20-21 -0.10523812029791202 1101 22-23 -0.16093245600345796 1101 24-25 -0.1423117373245546 1101 26-27 -0.3051727507915345 1101 28-29 -0.4621369972868905 1101 30-31 -0.24632124767278896 1101 32-33 -0.11034279200952568 1101 34-35 -0.15086781012057315 1101 36-37 -0.12383500314455631 1101 38-39 -0.035936071119593294 1101 40-41 0.049877637037184286 1101 42-43 -0.010851695309490594 1101 44-45 -0.11548882047357978 1101 46-47 -0.1525943918348318 1101 48-49 -0.09699641490121991 1101 50-51 -0.039859443368918335 1101 52-53 -0.1295683167916053 1101 54-55 -0.0971365091427927 1101 56-57 -0.16144479154041846 1101 58-59 -0.1470674951602433 1101 60-61 -0.0347467324822901 1101 62-63 -0.11744798982012838 1101 64-65 -0.15336820246656657 1101 66-67 -0.02763771763132894 1101 68-69 -0.17478754756277226 1101 70-71 -0.2674248414140479 1101 72-73 -0.23738085154037947 1101 74-75 -0.3000884119699272 1101 76-77 -0.2682904472348504 1101 78-79 -0.34290900466134744 1101 80-81 -0.39614957978153953 1101 82-83 -0.39372452510700384 1101 84-85 -0.28852967553401143 1101 86-87 -0.4656385539918091 1101 88-89 -0.5171652159979807 1101 90-91 -0.5004236386771268 1101 92-93 -0.5856856668675476 1101 94-95 -0.46244589025814875 1101 96-97 -0.5692262513863113 1101 98-99 -0.5373556794747607 1101 100-101 0.19070030872930488 1102 1 -0.138827204863901 1102 2 -0.07240041671900599 1102 3 -0.07469714634889613 1102 4 -0.0953692930057386 1102 5 -0.12910338431651525 1102 6 -0.19451350599043593 1102 7 -0.20783868486637402 1102 8 -0.16434989074548412 1102 9 -0.2543914682393691 1102 10-11 -0.15686842520538136 1102 12-13 -0.18265527922261526 1102 14-15 -0.157550277041274 1102 16-17 -0.3013148458621657 1102 18-19 -0.3156430190396904 1102 20-21 -0.11734532606170944 1102 22-23 -0.12622164965618055 1102 24-25 -0.21390301717198668 1102 26-27 -0.20302758159863998 1102 28-29 -0.21745832419058786 1102 30-31 -0.20339544005297228 1102 32-33 -0.15308068850368528 1102 34-35 -0.24553964396946526 1102 36-37 -0.32639574070694266 1102 38-39 -0.25685374115617776 1102 40-41 -0.21114807068477148 1102 42-43 -0.21701043760552352 1102 44-45 -0.18581310170792875 1102 46-47 -0.21528769867823172 1102 48-49 -0.1368626683727001 1102 50-51 -0.11534763669762782 1102 52-53 -0.11162891476606518 1102 54-55 -0.2267867769385674 1102 56-57 -0.28417968325046417 1102 58-59 -0.20283836243287112 1102 60-61 -0.17211290466762819 1102 62-63 -0.17064029263957536 1102 64-65 -0.1894266769107631 1102 66-67 -0.18222376937864482 1102 68-69 -0.06206817309603707 1102 70-71 -0.17818830323927415 1102 72-73 -0.23192952287642044 1102 74-75 -0.21314145201540313 1102 76-77 -0.19566700162200235 1102 78-79 -0.2056555118801171 1102 80-81 -0.14454672927291057 1102 82-83 -0.13748253459449344 1102 84-85 -0.3367824720574326 1102 86-87 -0.2635426610159399 1102 88-89 -0.14552387510185483 1102 90-91 -0.32304400102269426 1102 92-93 -0.2607507798664912 1102 94-95 -0.21588793772700754 1102 96-97 -0.16603904997271712 1102 98-99 0.007041519348891967 1102 100-101 0.5030783935382175 1103 1 -0.06652342156414903 1103 2 -0.05611096070825994 1103 3 -0.058515145005713975 1103 4 -0.07479463439816669 1103 5 -0.06669147475752624 1103 6 -0.1235885003938435 1103 7 -0.0833128285869904 1103 8 -0.1209373095948294 1103 9 -0.15248280470679276 1103 10-11 -0.11850487024680234 1103 12-13 -0.1612894856251188 1103 14-15 -0.2097218687135367 1103 16-17 -0.16910083198260395 1103 18-19 -0.16850254444823776 1103 20-21 -0.26975231061510385 1103 22-23 -0.2509293953468017 1103 24-25 -0.21658636169851064 1103 26-27 -0.2524265070519647 1103 28-29 -0.3881127902752013 1103 30-31 -0.3545891942504227 1103 32-33 -0.27924735517035515 1103 34-35 -0.17351166098311666 1103 36-37 -0.3077540348640966 1103 38-39 -0.21195638275008832 1103 40-41 -0.22633466123771484 1103 42-43 -0.16777907427442784 1103 44-45 -0.315613750913208 1103 46-47 -0.09560413028780346 1103 48-49 -0.2263962479563162 1103 50-51 -0.09579043947353938 1103 52-53 -0.19243336965748625 1103 54-55 -0.3361562642902598 1103 56-57 -0.38988552609801275 1103 58-59 -0.3379414517545598 1103 60-61 -0.3557784520148388 1103 62-63 -0.1438787076832284 1103 64-65 -0.23006603218276211 1103 66-67 -0.16901445664078807 1103 68-69 -0.26568738061786235 1103 70-71 -0.15799801893212617 1103 72-73 -0.15905085710520694 1103 74-75 -0.05184606361155275 1103 76-77 -0.015356390473581172 1103 78-79 -0.11485419108316108 1103 80-81 -0.32703911790062534 1103 82-83 -0.2913900788076127 1103 84-85 -0.34560841788237084 1103 86-87 -0.40157400196064685 1103 88-89 -0.18392481533019733 1103 90-91 -0.24084879170995777 1103 92-93 -0.127200970150799 1103 94-95 -0.2775978101245933 1103 96-97 -0.13422541666177068 1103 98-99 0.020819334437316428 1103 100-101 0.7798118835762722 1104 1 -0.06638425245687074 1104 2 -0.056935887489906634 1104 3 -0.05132835685081005 1104 4 -0.03317395970460524 1104 5 -0.08485591775568224 1104 6 -0.07433738855958438 1104 7 -0.06430306613987113 1104 8 -0.060666998281234896 1104 9 -0.013496146717855595 1104 10-11 -0.04068623481996525 1104 12-13 -0.05818036316065189 1104 14-15 -0.017642630184404595 1104 16-17 -0.14683160538535844 1104 18-19 -0.09530702432675042 1104 20-21 -0.11853590970394379 1104 22-23 -0.14952886383579056 1104 24-25 -0.095241259192818 1104 26-27 -0.24229471401584846 1104 28-29 -0.036320893106314145 1104 30-31 -0.10881389324163848 1104 32-33 -0.12346793223944275 1104 34-35 -0.15400227818672363 1104 36-37 -0.14860255422627944 1104 38-39 -0.11226232699566907 1104 40-41 -0.04495983750412336 1104 42-43 -6.991849154971419E-4 1104 44-45 -0.019018512817972066 1104 46-47 -0.08157446329573048 1104 48-49 0.1441320631403329 1104 50-51 -0.102785124363443 1104 52-53 -0.05273280560498961 1104 54-55 0.07330783159866172 1104 56-57 0.07183656957396778 1104 58-59 -0.06942387934974192 1104 60-61 -0.003632015308042469 1104 62-63 -0.028270507227652786 1104 64-65 -0.10053983628299079 1104 66-67 -0.038158832386535835 1104 68-69 0.0062693391999033565 1104 70-71 -0.018132523759113894 1104 72-73 0.07877396120480284 1104 74-75 0.2004784292835602 1104 76-77 0.20226401304089947 1104 78-79 0.09001168374057045 1104 80-81 0.06352162621478996 1104 82-83 -0.03474239467701068 1104 84-85 -0.09024953947907477 1104 86-87 -0.06179181300305814 1104 88-89 0.060104797426067336 1104 90-91 0.08987992073173956 1104 92-93 0.1937753830328468 1104 94-95 0.24633710689461097 1104 96-97 0.19346137149312526 1104 98-99 0.6000314507189053 1104 100-101 0.9894707945210612 1105 1 -0.04604021403761749 1105 2 -0.05475388817790616 1105 3 -0.022638873263012727 1105 4 -0.06410225938449798 1105 5 -0.10148657376201697 1105 6 -0.0872473998577945 1105 7 -0.12989536879047137 1105 8 -0.08806913324650623 1105 9 -0.06993144271138618 1105 10-11 -0.10089301158800623 1105 12-13 -0.13359481326980216 1105 14-15 -0.1677394269675503 1105 16-17 -0.18893454820621258 1105 18-19 -0.15027800665149726 1105 20-21 -0.06773983933187822 1105 22-23 -0.10294246831475107 1105 24-25 -0.22946903317865974 1105 26-27 -0.13878522054064746 1105 28-29 -0.15680215686529664 1105 30-31 -0.17131630288186273 1105 32-33 -0.0964676264754658 1105 34-35 -0.2099712387098407 1105 36-37 -0.24085954848404612 1105 38-39 -0.18363338745417224 1105 40-41 -0.22945406830478277 1105 42-43 -0.05697385602697125 1105 44-45 -0.15838275868041052 1105 46-47 -0.10500786073306045 1105 48-49 0.08312344354352064 1105 50-51 -0.08752832373127717 1105 52-53 0.006765897381121988 1105 54-55 0.1607183117955131 1105 56-57 0.06574558647172069 1105 58-59 0.13804328733306193 1105 60-61 0.049569102957256916 1105 62-63 0.26074748201155273 1105 64-65 0.19885618639288793 1105 66-67 0.19174060298069406 1105 68-69 0.20785938669425263 1105 70-71 0.19506838518120873 1105 72-73 0.22543366318797808 1105 74-75 0.37275742615981144 1105 76-77 0.32273107260485645 1105 78-79 0.2041570221320228 1105 80-81 0.17557553038730944 1105 82-83 0.1656567432031082 1105 84-85 0.2639951812862087 1105 86-87 0.1963286345123869 1105 88-89 0.49606107242825104 1105 90-91 0.6117638327293804 1105 92-93 0.716422156421789 1105 94-95 0.6726247038235549 1105 96-97 0.6535587973191923 1105 98-99 0.7263333372816412 1105 100-101 1.3139629064402918 1106 1 -0.14893167896092763 1106 2 -0.0578271267561874 1106 3 -0.04834470963615445 1106 4 -0.036474125904526034 1106 5 -0.05897384622788593 1106 6 -0.026296791540204367 1106 7 -0.049486638914366665 1106 8 -0.1044684154560045 1106 9 -0.03547774227007494 1106 10-11 -0.1862053989901895 1106 12-13 -0.17049715802921384 1106 14-15 -0.16193432230786442 1106 16-17 -0.06016844363746543 1106 18-19 -0.07265098634271538 1106 20-21 -0.01273982327118972 1106 22-23 -0.15254864385619982 1106 24-25 -0.07083931478659622 1106 26-27 -0.11736575781133496 1106 28-29 -0.2522364824790486 1106 30-31 -0.18363323744988236 1106 32-33 -0.12354980763969792 1106 34-35 -0.05270313358970924 1106 36-37 -0.11740725794880547 1106 38-39 -0.31899716349033014 1106 40-41 -0.1893811794062401 1106 42-43 -0.4514842717094538 1106 44-45 -0.26864485677438665 1106 46-47 -0.3949545071580971 1106 48-49 -0.5491135952394757 1106 50-51 -0.32501802412873104 1106 52-53 -0.18619706636128086 1106 54-55 -0.22991433606436118 1106 56-57 -0.30455579271968247 1106 58-59 -0.3271957548257731 1106 60-61 -0.2613046079378947 1106 62-63 -0.17618245388640474 1106 64-65 -0.18411463157526953 1106 66-67 -0.1548623585420117 1106 68-69 -0.1272759732604527 1106 70-71 -0.10512142988949336 1106 72-73 -0.14650535142403243 1106 74-75 -0.16247554949297793 1106 76-77 -0.0892941225019328 1106 78-79 -0.13374681117540987 1106 80-81 -0.16470201586440325 1106 82-83 -0.2210688953224036 1106 84-85 -0.23925129109645482 1106 86-87 -0.15724820603309553 1106 88-89 0.05424359506467269 1106 90-91 0.1391521082787932 1106 92-93 0.24617185018894716 1106 94-95 0.3725581129263702 1106 96-97 0.3724490686571613 1106 98-99 0.4888474028364591 1106 100-101 1.1830248996635717 1107 1 -0.051496278234125725 1107 2 -0.1137723311154133 1107 3 -0.06561709800141102 1107 4 -0.0677575605815548 1107 5 -0.062130733281193784 1107 6 -0.14208767285329316 1107 7 -0.1618599983983131 1107 8 -0.12785952078317564 1107 9 -0.08695417180378229 1107 10-11 -0.0855058632954595 1107 12-13 -0.06763936976944507 1107 14-15 -0.13372666844872327 1107 16-17 -0.10365892532917798 1107 18-19 -0.159025384567407 1107 20-21 -0.09799593855287014 1107 22-23 -0.06813280481387096 1107 24-25 -0.18527239281403496 1107 26-27 -0.18344438192783485 1107 28-29 -0.16151547150658985 1107 30-31 -0.08659929024512536 1107 32-33 -0.21680131081417642 1107 34-35 -0.26177283278053665 1107 36-37 -0.24367508749566724 1107 38-39 -0.2325652870863948 1107 40-41 -0.1101064255541715 1107 42-43 -0.18555116960710194 1107 44-45 -0.14622331728195803 1107 46-47 -0.19405480887106563 1107 48-49 -0.0502693032360213 1107 50-51 0.03954355589223724 1107 52-53 -0.04790490226259436 1107 54-55 0.008157042561776962 1107 56-57 -0.03197622454224103 1107 58-59 -0.12824383507134485 1107 60-61 0.12371244503911072 1107 62-63 0.11647481763951362 1107 64-65 0.13432258085927629 1107 66-67 0.17452145861906132 1107 68-69 0.24079093781973882 1107 70-71 0.188999663954327 1107 72-73 0.31453835176602496 1107 74-75 0.3828247407578651 1107 76-77 0.26503338112522457 1107 78-79 0.22625851993968027 1107 80-81 0.3188531363198308 1107 82-83 0.07920409530692041 1107 84-85 0.2019607645075645 1107 86-87 0.2525034558347876 1107 88-89 0.20747902445794963 1107 90-91 0.23332929501628996 1107 92-93 0.36507940058736565 1107 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0.34364654635889025 2311 14-15 0.2923028689176519 2311 16-17 0.40246338590844744 2311 18-19 0.31524083386260315 2311 20-21 0.45476343151382537 2311 22-23 0.3935276261424505 2311 24-25 0.3941880011200851 2311 26-27 0.3681357118385904 2311 28-29 0.480573116771275 2311 30-31 0.36937257486501807 2311 32-33 0.3371741840600322 2311 34-35 0.30127744446416216 2311 36-37 0.41709978822436256 2311 38-39 0.48763407202252296 2311 40-41 0.44386298993669016 2311 42-43 0.4063341191288643 2311 44-45 0.39343597422422505 2311 46-47 0.34051306111648927 2311 48-49 0.3412146965714271 2311 50-51 0.3776298503815312 2311 52-53 0.30327402664435965 2311 54-55 0.3940838656447738 2311 56-57 0.5100215203787428 2311 58-59 0.383655999519803 2311 60-61 0.42995468141844384 2311 62-63 0.5169998530364168 2311 64-65 0.4151903396313301 2311 66-67 0.4610115303657025 2311 68-69 0.5784160676579972 2311 70-71 0.5885936852157982 2311 72-73 0.5762829759782448 2311 74-75 0.5336264501315817 2311 76-77 0.4393699963330171 2311 78-79 0.5167407614405768 2311 80-81 0.5327212519144666 2311 82-83 0.6641396563248207 2311 84-85 0.6653493532320702 2311 86-87 0.8324629795485983 2311 88-89 0.766580432046112 2311 90-91 0.7345145266308748 2311 92-93 0.5584307140071196 2311 94-95 0.6323841988709127 2311 96-97 0.7368218097250363 2311 98-99 0.744564211998533 2311 100-101 0.4650665062649253 2312 1 0.08340587136514444 2312 2 0.25191934232204005 2312 3 0.3056767741862103 2312 4 -0.04528958389311555 2312 5 0.07959135352530211 2312 6 0.09323978243444486 2312 7 0.21235383807967168 2312 8 0.1252445085869951 2312 9 0.2028505286265414 2312 10-11 0.2987678570259291 2312 12-13 0.3636903123546773 2312 14-15 0.4306670201753562 2312 16-17 0.42382846094668736 2312 18-19 0.33280553635983523 2312 20-21 0.3923082954455026 2312 22-23 0.565651412734006 2312 24-25 0.48786313325098263 2312 26-27 0.5233815737561116 2312 28-29 0.5604680178056043 2312 30-31 0.4979613108000862 2312 32-33 0.4873233519003506 2312 34-35 0.44716510669364595 2312 36-37 0.5393924297823744 2312 38-39 0.6261193748256701 2312 40-41 0.5179532175501578 2312 42-43 0.4438320368366817 2312 44-45 0.388845845046383 2312 46-47 0.5082292030455235 2312 48-49 0.47573001466994924 2312 50-51 0.40962370194060327 2312 52-53 0.5423090545849263 2312 54-55 0.7051518165178194 2312 56-57 0.5715892213767333 2312 58-59 0.46189188157877226 2312 60-61 0.544706396470005 2312 62-63 0.5261414943369758 2312 64-65 0.4971258870091617 2312 66-67 0.5917734221470923 2312 68-69 0.6509691850387043 2312 70-71 0.7225070315729241 2312 72-73 0.7075855065311529 2312 74-75 0.46198726972177795 2312 76-77 0.3840628014456122 2312 78-79 0.5753326776037078 2312 80-81 0.5080063366448293 2312 82-83 0.6609836585661242 2312 84-85 0.7172552184812666 2312 86-87 0.6165270081403627 2312 88-89 0.7068731644677833 2312 90-91 0.7853936325324682 2312 92-93 0.762708120758294 2312 94-95 0.8837759272491468 2312 96-97 0.7216786237422674 2312 98-99 0.7206829708009543 2312 100-101 0.8621906714550605 2313 1 -0.34455526869790276 2313 2 0.006638508506178198 2313 3 0.16291797601339653 2313 4 0.16365334861669112 2313 5 0.1336864445630681 2313 6 0.22867052296072643 2313 7 0.2643708232176749 2313 8 0.23402659698691508 2313 9 0.2792836496456488 2313 10-11 0.34368196627125513 2313 12-13 0.4237784549268966 2313 14-15 0.29931528627584925 2313 16-17 0.4141263442381984 2313 18-19 0.4469019141504873 2313 20-21 0.48040904819593777 2313 22-23 0.4450440653750647 2313 24-25 0.5915335958386478 2313 26-27 0.29671362033656834 2313 28-29 0.6475039825485709 2313 30-31 0.5418492346243156 2313 32-33 0.40902122232434124 2313 34-35 0.50103823285977 2313 36-37 0.28982027511467834 2313 38-39 0.3495414941076618 2313 40-41 0.2192078016072898 2313 42-43 0.3662665732839585 2313 44-45 0.22271639770674767 2313 46-47 0.3328576531516845 2313 48-49 0.14006765862542636 2313 50-51 -0.04081379403829288 2313 52-53 -0.01760215946640642 2313 54-55 0.01871740874583594 2313 56-57 0.1579785927979458 2313 58-59 0.34342054399915867 2313 60-61 0.42488396836731823 2313 62-63 0.4535268124866221 2313 64-65 0.3132842862885781 2313 66-67 0.2969558193677031 2313 68-69 0.41110879772608655 2313 70-71 0.4073522995392054 2313 72-73 0.41167738068671866 2313 74-75 0.4643227261973699 2313 76-77 0.4324833122865037 2313 78-79 0.34040377070027716 2313 80-81 0.38042478868111473 2313 82-83 0.533588689769882 2313 84-85 0.38397013838089933 2313 86-87 0.40164892797566054 2313 88-89 0.5647609596167236 2313 90-91 0.47442084735585865 2313 92-93 0.7262576285753148 2313 94-95 0.5889711919985494 2313 96-97 0.624798999474109 2313 98-99 0.7990751730785028 2313 100-101 0.9711271702004751 2314 1 -0.5341995110812263 2314 2 -0.11789541300507977 2314 3 -0.20415118740853444 2314 4 -0.14903373922737728 2314 5 0.14871724137910292 2314 6 0.17230784825162715 2314 7 0.2842030724730762 2314 8 0.3334466715594715 2314 9 0.3173970539504509 2314 10-11 0.31492858087217 2314 12-13 0.30876116219584304 2314 14-15 0.4385095800497183 2314 16-17 0.3748332080645085 2314 18-19 0.44407637192396265 2314 20-21 0.4372194208336566 2314 22-23 0.4682007577745182 2314 24-25 0.6306344863580264 2314 26-27 0.5301176625593413 2314 28-29 0.5613094712088937 2314 30-31 0.6113737032760795 2314 32-33 0.6004528804009084 2314 34-35 0.612556654682372 2314 36-37 0.622537484570536 2314 38-39 0.5411879046468542 2314 40-41 0.601385597890669 2314 42-43 0.5068375334994215 2314 44-45 0.6816377214071636 2314 46-47 0.6161208402657863 2314 48-49 0.6063899711249547 2314 50-51 0.6023379468633294 2314 52-53 0.11588759618467037 2314 54-55 0.12095739884284029 2314 56-57 0.5233194208984528 2314 58-59 0.674406604782547 2314 60-61 0.16212260805583156 2314 62-63 0.25291987888920886 2314 64-65 0.5736936851317296 2314 66-67 0.7672552724522603 2314 68-69 0.7336341741524564 2314 70-71 0.9086333120454881 2314 72-73 0.7131776470172895 2314 74-75 0.9196138892741921 2314 76-77 0.9049527968055706 2314 78-79 0.6309685415434565 2314 80-81 0.7395621777413055 2314 82-83 0.7053531485186539 2314 84-85 0.7159033606806453 2314 86-87 0.7957075056954217 2314 88-89 0.8927995662952419 2314 90-91 1.0824704073477562 2314 92-93 1.2107860734298832 2314 94-95 1.1560311651404263 2314 96-97 0.924472463196885 2314 98-99 1.1127056713042158 2314 100-101 0.9993413299838103 2315 1 0.2811063787430754 2315 2 0.22146968445302662 2315 3 0.21090881812792617 2315 4 0.20942809985682231 2315 5 0.11129316425936508 2315 6 0.1498892261806617 2315 7 0.20812956772637392 2315 8 0.07718557826999728 2315 9 0.13103793262039432 2315 10-11 0.08332308674245326 2315 12-13 0.07438946982802719 2315 14-15 0.3596352741436064 2315 16-17 0.342919137295425 2315 18-19 0.38014645708785366 2315 20-21 0.3399343253295939 2315 22-23 0.4715490992768494 2315 24-25 0.39337446779457963 2315 26-27 0.5893476864964171 2315 28-29 0.5856821406003689 2315 30-31 0.4847310822472721 2315 32-33 0.28067507186498375 2315 34-35 0.42365116543777503 2315 36-37 0.5360362877165485 2315 38-39 0.38782165104407085 2315 40-41 0.30153093016012633 2315 42-43 0.5203937367854792 2315 44-45 0.4792533765829532 2315 46-47 0.5503182967475198 2315 48-49 0.28775812992387273 2315 50-51 0.4582249215095686 2315 52-53 0.4989806088371296 2315 54-55 0.43674772956534724 2315 56-57 0.38805456606787914 2315 58-59 0.4120838938675604 2315 60-61 0.4509450502892989 2315 62-63 0.29543465637207333 2315 64-65 0.3939477927020292 2315 66-67 0.4557658813008416 2315 68-69 0.5197596239904314 2315 70-71 0.6598668044165663 2315 72-73 0.7130351644001678 2315 74-75 0.6597215247141044 2315 76-77 0.556314116438859 2315 78-79 0.5226980362593885 2315 80-81 0.5144823874757201 2315 82-83 0.33574509784369155 2315 84-85 0.5006304454048767 2315 86-87 0.6474014204663909 2315 88-89 0.8444714980338581 2315 90-91 0.92927716835149 2315 92-93 0.8198789069613355 2315 94-95 0.971402189875409 2315 96-97 1.0524727400202565 2315 98-99 1.237583543346684 2315 100-101 0.943589917370435 2316 1 -0.20688945244516432 2316 2 -0.18626082446847647 2316 3 0.04288812474905512 2316 4 0.18821989784524362 2316 5 0.10653105021976472 2316 6 0.09969397008753589 2316 7 0.2718452095601478 2316 8 0.30942417996012495 2316 9 0.24553899365832876 2316 10-11 0.39592746399480205 2316 12-13 0.35353976051474945 2316 14-15 0.4466827634390569 2316 16-17 0.3587829100700759 2316 18-19 0.4377831049613832 2316 20-21 0.4412885613194959 2316 22-23 0.4065715129184113 2316 24-25 0.4497256274023087 2316 26-27 0.5688677708771834 2316 28-29 0.5935316238937318 2316 30-31 0.44413696378772016 2316 32-33 0.5308430172183876 2316 34-35 0.369497421669017 2316 36-37 0.48441898965906915 2316 38-39 0.69056904867589 2316 40-41 0.4524714241392047 2316 42-43 0.4562904080144037 2316 44-45 0.376174169159448 2316 46-47 0.25178052003008133 2316 48-49 -0.06904883651216664 2316 50-51 0.35195161320584845 2316 52-53 0.49622285195921734 2316 54-55 0.4061330181802205 2316 56-57 0.5441417218583879 2316 58-59 0.4688855237222782 2316 60-61 0.41120792400695194 2316 62-63 0.4178542623440151 2316 64-65 0.40681680160470535 2316 66-67 0.4791526641584838 2316 68-69 0.39021725153472886 2316 70-71 0.6661890837018447 2316 72-73 0.4685177169184058 2316 74-75 0.4511789136818187 2316 76-77 0.27906459045285104 2316 78-79 0.2095976708605285 2316 80-81 0.5668577939976451 2316 82-83 0.47519938531591066 2316 84-85 0.639020555777936 2316 86-87 0.4082425284662392 2316 88-89 0.40445938085508715 2316 90-91 0.3646480293617955 2316 92-93 0.4582658781689126 2316 94-95 0.5671106367418943 2316 96-97 1.0942173626297809 2316 98-99 1.0342739211766414 2316 100-101 0.9077814566916231 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 11.0 4 21.0 5 67.0 6 119.0 7 290.0 8 683.0 9 1228.0 10 2076.0 11 3214.0 12 4839.0 13 7040.0 14 9165.0 15 12389.0 16 16523.0 17 22415.0 18 20629.0 19 13666.0 20 12360.0 21 12535.0 22 13320.0 23 14611.0 24 17156.0 25 20518.0 26 25144.0 27 30939.0 28 38816.0 29 48493.0 30 60105.0 31 75275.0 32 95004.0 33 122937.0 34 167188.0 35 235583.0 36 357503.0 37 536257.0 38 433834.0 39 42109.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28711437574071 19.387935881308422 14.623688968527155 22.701260774423712 2 16.046493486231377 20.402640672221082 39.56449037152187 23.98637547002567 3 19.91502251151646 24.168262217635984 29.398193339145624 26.518521931701933 4 13.067592753146945 16.531524813192206 36.52956852017346 33.87131391348739 5 13.653902275569537 37.602394162047325 34.03940472543046 14.704298836952681 6 32.77223292659415 36.229374548061955 16.782245589953707 14.216146935390189 7 29.2752783170383 32.43921850135829 19.94812589958154 18.33737728202187 8 26.888358318509564 35.3471834028747 18.934167910569926 18.830290368045812 9 26.35640021066527 16.604845382117784 19.853463952269685 37.18529045494726 10-11 25.295000214222256 26.902192584999753 26.785744600184476 21.017062600593515 12-13 26.154103607494626 23.854869958078897 27.761645121351307 22.229381313075173 14-15 22.631703183462694 25.348545532517914 26.113439935135148 25.90631134888425 16-17 22.18761658435281 28.89135581211018 26.631089618834093 22.28993798470292 18-19 22.101846878035236 27.22482859195002 29.153596082887002 21.519728447127743 20-21 22.779536689992632 26.201372072785418 29.50918467446516 21.50990656275679 22-23 22.387495937824873 26.16287341696429 29.439068181487478 22.010562463723364 24-25 22.339331406528864 26.326810208951372 29.53079461586079 21.80306376865897 26-27 22.124566627897693 26.554132230152288 29.452306585437082 21.868994556512934 28-29 21.913291625951274 26.82118810460462 29.451885775263165 21.813634494180942 30-31 22.085469753823325 26.421567171162017 29.46559627882387 22.02736679619079 32-33 21.928306711176372 26.524322097986836 29.436692700723142 22.110678490113646 34-35 22.450786498506307 26.576099192139907 29.21527589996552 21.757838409388267 36-37 22.408148689616873 26.392499959075472 29.26895861529995 21.930392736007704 38-39 22.143440050346577 26.70931197457126 29.24537529743728 21.90187267764488 40-41 22.359571611642142 26.568477324854435 29.18752893771724 21.88442212578618 42-43 22.26081188124015 26.615438068987206 28.949628468698414 22.17412158107423 44-45 22.31373391486024 26.766654749689934 28.810257950337935 22.10935338511189 46-47 22.490983469728185 27.013193256286105 28.373908224797468 22.12191504918824 48-49 22.58974797368058 27.111593986743287 28.18694885789269 22.11170918168344 50-51 22.52473356622921 27.270946987288543 28.084485990072217 22.11983345641003 52-53 22.75023271398875 27.340811708009525 27.802319015612753 22.106636562388974 54-55 22.55432047717382 27.25431445470151 27.86383715558229 22.327527912542386 56-57 22.544983624129824 27.470840522912273 27.828146933773418 22.156028919184482 58-59 22.675356002889167 27.20896402563067 27.860967386897766 22.25471258458239 60-61 22.620426205110856 27.231619030527465 28.19264797598448 21.955306788377193 62-63 22.461820961194665 27.285821281315343 28.20816897844723 22.044188779042766 64-65 22.344160445129415 27.292146898204457 28.05310042129013 22.310592235376003 66-67 22.369301235576884 27.41370787452401 27.829682866308797 22.387308023590307 68-69 22.38179079224613 27.28384073673025 27.973838218609277 22.360530252414346 70-71 22.43514423821182 27.273715707779942 27.87311337971041 22.418026674297824 72-73 22.527037465032272 27.266056254741688 27.782998601088813 22.423907679137226 74-75 22.31602862816569 27.604163508910634 27.621927781260574 22.45788008166311 76-77 22.48898271550447 27.375531867164472 27.66590395490502 22.46958146242604 78-79 22.473219197378246 27.3785835225966 27.662589574170788 22.485607705854367 80-81 22.4200925884571 27.5470768469643 27.60986820685701 22.42296235772159 82-83 22.46650184949442 27.540137050335794 27.60903205648865 22.38432904368113 84-85 22.375286245599582 27.45389098991514 27.526464139547397 22.64435862493788 86-87 22.394482445301602 27.39897504796758 27.57409156663906 22.63245094009176 88-89 22.401939801953624 27.356918789992346 27.45604706743997 22.785094340614055 90-91 22.417305720133186 27.325826518357488 27.589020474638424 22.667847286870902 92-93 22.432016155289322 27.1195076290775 27.63598133136027 22.812494884272905 94-95 22.32162669806436 27.110442037341752 27.60408267035614 22.963848594237746 96-97 22.212110666364328 27.097831222840775 27.70634344179038 22.983714669004517 98-99 22.130441196977443 27.17824713578665 27.4322824986409 23.259029168595006 100-101 22.049416956556275 27.18714812292815 27.66661553048333 23.096819390032245 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 300.0 1 224.0 2 221.0 3 322.0 4 294.0 5 271.5 6 514.0 7 1163.5 8 1910.0 9 2304.0 10 2242.0 11 2153.0 12 2334.5 13 2579.0 14 2850.0 15 3179.0 16 3384.0 17 3582.5 18 3916.0 19 4199.5 20 4625.0 21 5412.0 22 6440.5 23 7685.5 24 9246.5 25 11276.5 26 14191.0 27 17677.5 28 20870.0 29 24372.5 30 28914.5 31 33680.0 32 38704.0 33 43039.5 34 47671.0 35 52346.5 36 57431.5 37 62959.5 38 66715.5 39 69320.0 40 71812.0 41 75976.0 42 79974.0 43 84069.5 44 88303.0 45 91463.5 46 92478.5 47 94665.5 48 99426.0 49 98895.5 50 94165.5 51 90130.5 52 86532.0 53 83801.5 54 81619.5 55 76248.5 56 68493.0 57 61252.5 58 55399.5 59 49258.0 60 41542.0 61 34236.5 62 28027.5 63 21685.0 64 16190.5 65 12442.0 66 9265.0 67 7333.0 68 5585.0 69 3786.0 70 2689.0 71 1928.0 72 1399.0 73 1071.0 74 820.5 75 635.5 76 430.5 77 230.5 78 137.5 79 100.0 80 71.0 81 45.5 82 25.0 83 20.0 84 15.0 85 7.0 86 5.5 87 4.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0718250556674496 2 0.0 3 0.0 4 0.0 5 0.001980544585090056 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.041927724673584E-5 12-13 0.005416183151062602 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.296433766749243E-4 24-25 9.094337380515564E-4 26-27 3.839831338439905E-4 28-29 0.0034154289273491782 30-31 4.244024110907263E-4 32-33 0.0 34-35 0.0 36-37 2.020963862336792E-5 38-39 6.467084359477734E-4 40-41 1.4146747036357545E-4 42-43 0.012873539803085365 44-45 2.2230602485704713E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.020963862336792E-5 82-83 5.05240965584198E-4 84-85 4.6482168833746214E-4 86-87 0.0 88-89 0.0 90-91 0.0091347566577623 92-93 0.003172913263868763 94-95 0.0 96-97 0.0 98-99 8.083855449347167E-5 100-101 0.011337607267709403 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2474067.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.2756120451733 #Duplication Level Percentage of deduplicated Percentage of total 1 77.98347797134912 46.22518386163008 2 12.976605608891164 15.383924794717052 3 3.9629428733630783 7.047175929559625 4 1.7581496952117113 4.168615970028363 5 0.9044599065565728 2.6806207265730553 6 0.5388809682315215 1.9165499526851415 7 0.3560558665226229 1.4773800587282102 8 0.24687114950572037 1.1706750786597644 9 0.18803824845647965 1.0031474038644175 >10 0.9791564208534115 10.559381869632041 >50 0.07120062962035628 2.9028709498692216 >100 0.03156910708773576 3.4166916697858247 >500 0.0021141627596242374 0.8111731735603198 >1k 2.7279519478937397E-4 0.2958724437991048 >5k 2.0459639609203044E-4 0.9407361169079065 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9854 0.39829155798933497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7867 0.31797845410007086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5550 0.22432698871938392 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2552 0.10314999553366985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.041927724673584E-5 0.0 0.0 0.0 0.0 8 4.041927724673584E-5 0.0 0.0 0.0 4.041927724673584E-5 9 4.041927724673584E-5 0.0 0.0 0.0 4.041927724673584E-5 10-11 4.041927724673584E-5 0.0 0.0 0.0 4.041927724673584E-5 12-13 4.041927724673584E-5 0.0 0.0 0.0 4.041927724673584E-5 14-15 4.041927724673584E-5 0.0 0.0 0.0 4.041927724673584E-5 16-17 1.0104819311683959E-4 0.0 0.0 0.0 4.041927724673584E-5 18-19 1.4146747036357545E-4 0.0 0.0 0.0 4.041927724673584E-5 20-21 1.6167710898694335E-4 0.0 0.0 0.0 4.041927724673584E-5 22-23 1.6167710898694335E-4 0.0 0.0 0.0 1.4146747036357545E-4 24-25 1.6167710898694335E-4 0.0 0.0 8.083855449347167E-5 2.4251566348041504E-4 26-27 1.6167710898694335E-4 0.0 0.0 1.6167710898694338E-4 2.4251566348041504E-4 28-29 1.6167710898694335E-4 0.0 0.0 0.0011317397629086036 2.4251566348041504E-4 30-31 1.6167710898694335E-4 0.0 0.0 0.0022230602485704713 2.4251566348041504E-4 32-33 1.6167710898694335E-4 0.0 0.0 0.003536686759089386 2.829349407271509E-4 34-35 1.8188674761031128E-4 0.0 0.0 0.004830103630984933 2.829349407271509E-4 36-37 2.020963862336792E-4 0.0 0.0 0.006972325325061932 2.829349407271509E-4 38-39 2.020963862336792E-4 0.0 0.0 0.010872785579371942 2.829349407271509E-4 40-41 2.020963862336792E-4 0.0 0.0 0.015419954269629724 2.829349407271509E-4 42-43 2.4251566348041504E-4 0.0 0.0 0.01917894705357616 2.829349407271509E-4 44-45 2.4251566348041504E-4 0.0 0.0 0.0214828458566401 2.829349407271509E-4 46-47 2.829349407271509E-4 0.0 0.0 0.025363096472326742 3.2335421797388675E-4 48-49 2.829349407271509E-4 0.0 0.0 0.03128452058897354 4.2440241109072634E-4 50-51 2.829349407271509E-4 0.0 0.0 0.039833197726658165 4.4461204971409427E-4 52-53 2.829349407271509E-4 0.0 0.0 0.05654656886818344 4.6482168833746214E-4 54-55 2.829349407271509E-4 0.0 0.0 0.0696222050575025 4.8503132696083007E-4 56-57 2.829349407271509E-4 0.0 0.0 0.10165448227554064 4.8503132696083007E-4 58-59 2.829349407271509E-4 0.0 0.0 0.15535149209782922 5.456602428309339E-4 60-61 2.829349407271509E-4 0.0 0.0 0.20632020070596307 5.658698814543018E-4 62-63 2.829349407271509E-4 0.0 0.0 0.23843331647849475 5.658698814543018E-4 64-65 2.829349407271509E-4 0.0 0.0 0.29089753834475784 5.658698814543018E-4 66-67 3.233542179738867E-4 0.0 0.0 0.38244720130861454 5.658698814543018E-4 68-69 3.233542179738867E-4 0.0 0.0 0.5082724113777032 5.658698814543018E-4 70-71 3.435638565972546E-4 0.0 0.0 0.6333296551791039 5.658698814543018E-4 72-73 3.6377349522062255E-4 0.0 0.0 0.7649752411717226 5.658698814543018E-4 74-75 3.6377349522062255E-4 0.0 0.0 0.916325224822125 5.658698814543018E-4 76-77 3.6377349522062255E-4 0.0 0.0 1.0387956348797345 6.062891587010376E-4 78-79 3.6377349522062255E-4 0.0 0.0 1.1993612137423926 6.062891587010376E-4 80-81 3.6377349522062255E-4 0.0 0.0 1.4072779758996017 6.264987973244056E-4 82-83 4.041927724673584E-4 0.0 0.0 1.6013713452384273 7.275469904412451E-4 84-85 4.2440241109072634E-4 0.0 0.0 1.8364902809826895 7.275469904412451E-4 86-87 4.4461204971409427E-4 0.0 0.0 2.1277718024612913 7.275469904412451E-4 88-89 4.4461204971409427E-4 0.0 0.0 2.497810285655158 7.67966267687981E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7575 0.0 34.822502 1 GTATCAA 11600 0.0 32.736965 1 ATCAACG 14295 0.0 26.24946 3 TCAACGC 14480 0.0 25.946892 4 TATCAAC 14650 0.0 25.678226 2 CAACGCA 14685 0.0 25.617542 5 GTACATG 10065 0.0 25.452158 1 AACGCAG 15125 0.0 24.966013 6 TACATGG 10130 0.0 24.522778 2 ACATGGG 10275 0.0 23.02104 3 ACGCAGA 17320 0.0 21.668402 7 CGCAGAG 17460 0.0 21.385824 8 GAGTACT 9615 0.0 21.195658 12-13 AGAGTAC 14550 0.0 20.53692 10-11 AGTACTT 9940 0.0 20.335371 12-13 GTACTTT 10125 0.0 19.86676 14-15 GTACCTG 2325 0.0 19.624468 1 GCAGAGT 18775 0.0 19.584328 9 TACCTGG 2205 0.0 19.387033 2 CATGGGA 6095 0.0 19.3266 4 >>END_MODULE