FastQCFastQC Report
Thu 26 May 2016
SRR937483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148134
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63420.5523745486154056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43380.3778304622979548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30500.2656484347645832No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19780.172279542283392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.048.767621
GTATCAA59450.045.1817171
ATCAACG74950.035.814463
TATCAAC75950.035.4680182
TCAACGC76300.035.057784
CAACGCA77600.034.4122545
AACGCAG79900.033.469446
ACGCAGA91250.029.0461227
CGCAGAG91750.028.7325218
GTATAGA2201.8189894E-1228.0922681
GCAGAGT95400.027.23499
GTACATG62600.027.1119041
TACATGG64750.025.4607582
ACATGGG65150.024.5022773
GAGTACT49700.024.4653512-13
AGTACTT52750.023.45595712-13
GTACTTT53900.022.82332214-15
AGAGTAC86300.021.98691710-11
CATGGGA43800.021.4778924
CAGAGTA93250.021.03582810-11
Produced by FastQC (version 0.11.5)