##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937483_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1148134 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73860194019165 33.0 31.0 34.0 30.0 34.0 2 31.841115235678064 33.0 31.0 34.0 30.0 34.0 3 31.867793306356226 33.0 31.0 34.0 30.0 34.0 4 35.50527900053478 37.0 35.0 37.0 33.0 37.0 5 35.509477116782534 37.0 35.0 37.0 33.0 37.0 6 35.5561694018294 37.0 35.0 37.0 33.0 37.0 7 35.49059169051696 37.0 35.0 37.0 33.0 37.0 8 35.478661027371366 37.0 35.0 37.0 33.0 37.0 9 37.07992098483278 39.0 37.0 39.0 33.0 39.0 10-11 36.96226311562936 39.0 37.0 39.0 33.0 39.0 12-13 36.829044780487294 39.0 37.0 39.0 33.0 39.0 14-15 37.93246607103352 40.0 38.0 41.0 33.0 41.0 16-17 37.869791766466285 40.0 38.0 41.0 33.0 41.0 18-19 37.81116359240298 40.0 38.0 41.0 33.0 41.0 20-21 37.705239109720644 40.0 37.0 41.0 32.0 41.0 22-23 37.75399822668783 40.0 37.5 41.0 32.5 41.0 24-25 37.7120780327035 40.0 37.0 41.0 32.0 41.0 26-27 37.63247713246015 40.0 37.0 41.0 32.0 41.0 28-29 37.48917852794186 40.0 37.0 41.0 32.0 41.0 30-31 37.2162987943916 39.0 36.5 41.0 31.5 41.0 32-33 36.96438438370434 39.0 36.0 41.0 31.0 41.0 34-35 36.98120733294198 39.0 36.0 41.0 31.0 41.0 36-37 36.84124588244926 39.0 36.0 41.0 31.0 41.0 38-39 36.69546542476749 39.0 36.0 41.0 30.5 41.0 40-41 36.45941370954958 39.0 35.0 41.0 30.0 41.0 42-43 36.16877254745526 39.0 35.0 41.0 30.0 41.0 44-45 35.885645316661645 39.0 35.0 40.5 29.0 41.0 46-47 35.41861446486212 39.0 35.0 40.0 26.5 41.0 48-49 35.04822128775909 38.5 35.0 40.0 25.5 41.0 50-51 34.05086862683275 37.5 33.5 39.5 23.5 40.5 52-53 34.12719203507605 38.0 33.5 39.5 24.0 40.5 54-55 34.42408116125819 38.0 34.0 40.0 23.5 41.0 56-57 34.17188934392675 38.0 34.0 40.0 23.0 41.0 58-59 33.90509905638192 38.0 33.5 40.0 22.0 41.0 60-61 33.57520768481727 37.5 33.0 40.0 20.5 41.0 62-63 33.1901886016789 37.0 33.0 40.0 18.5 41.0 64-65 32.761238235258254 36.0 32.0 39.0 17.5 41.0 66-67 32.318384439446966 36.0 31.5 39.0 13.0 40.5 68-69 31.824679436372406 35.0 31.0 39.0 8.0 40.0 70-71 31.365274872096812 35.0 31.0 38.0 7.0 40.0 72-73 30.887902892867906 35.0 30.0 37.5 4.0 39.5 74-75 30.355519042202392 34.5 29.5 37.0 2.0 39.0 76-77 29.847468588161313 34.0 29.0 36.0 2.0 39.0 78-79 29.31906728657108 34.0 29.0 36.0 2.0 38.5 80-81 28.757047957816773 34.0 28.5 35.0 2.0 37.0 82-83 28.216639782464416 34.0 27.0 35.0 2.0 37.0 84-85 27.667428627668897 33.0 26.0 35.0 2.0 36.0 86-87 27.092679513018517 33.0 25.5 35.0 2.0 36.0 88-89 26.568452375767983 32.5 25.0 35.0 2.0 35.5 90-91 26.01015647999275 32.0 23.5 35.0 2.0 35.0 92-93 25.364858979875173 32.0 20.5 34.0 2.0 35.0 94-95 24.57620626163845 31.0 16.5 34.0 2.0 35.0 96-97 23.7013083838646 31.0 4.5 34.0 2.0 35.0 98-99 22.495954304985307 31.0 2.0 34.0 2.0 35.0 100-101 19.424243163254463 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.059858898036626584 1101 2 0.06195901118953273 1101 3 0.012001360014330942 1101 4 -0.10263027863607732 1101 5 6.696617516368519E-4 1101 6 -0.07678612405355523 1101 7 0.02994508332727719 1101 8 -0.016802439530387403 1101 9 -0.012661223770557228 1101 10-11 0.12797391081813458 1101 12-13 0.09813475310316733 1101 14-15 0.28811269674405793 1101 16-17 0.2820859304464918 1101 18-19 0.18544602745495808 1101 20-21 0.21888474657439616 1101 22-23 0.2722072915760876 1101 24-25 0.20160255543862604 1101 26-27 0.2043178260923426 1101 28-29 0.07203766548557411 1101 30-31 0.06794891251909263 1101 32-33 0.15823434710986106 1101 34-35 0.22299309093757103 1101 36-37 0.2302105243331667 1101 38-39 0.2581484574798978 1101 40-41 0.31768374823895584 1101 42-43 0.42627179145971894 1101 44-45 0.5611149784232552 1101 46-47 0.8157916715382214 1101 48-49 0.8962346597108066 1101 50-51 0.9038798229897296 1101 52-53 0.9643439375561016 1101 54-55 1.051145293544593 1101 56-57 1.0011007674949894 1101 58-59 0.9977066908287 1101 60-61 0.9935368995982472 1101 62-63 1.06042627531874 1101 64-65 1.0566062337423716 1101 66-67 1.2925190266267848 1101 68-69 1.2556589088985355 1101 70-71 1.0901077428248058 1101 72-73 1.0140241237546839 1101 74-75 1.3145509388547438 1101 76-77 1.3644290797176808 1101 78-79 1.395526865122715 1101 80-81 1.4069557716311323 1101 82-83 1.167454060754718 1101 84-85 1.3691994346753873 1101 86-87 1.442462790520576 1101 88-89 1.4075943697834106 1101 90-91 1.3041009600503202 1101 92-93 1.4618500939748458 1101 94-95 1.3469248551472326 1101 96-97 1.3524775242449856 1101 98-99 1.7368102500400973 1101 100-101 1.8056562397974503 1102 1 0.16540417106777738 1102 2 0.2380685598935024 1102 3 0.12784225137239957 1102 4 0.21312702873254352 1102 5 0.14091362731349477 1102 6 0.09849084376468653 1102 7 -0.08154426450673213 1102 8 0.007218180409765296 1102 9 0.13867679103300645 1102 10-11 0.06678247472633814 1102 12-13 0.1305391730858787 1102 14-15 0.2364446593766445 1102 16-17 0.23972533454632128 1102 18-19 0.22418528950034755 1102 20-21 0.16010123869238413 1102 22-23 0.2672138191789344 1102 24-25 0.16742830199126502 1102 26-27 0.2187942471639417 1102 28-29 0.2126530621932048 1102 30-31 0.36153729204408336 1102 32-33 0.33920144837492927 1102 34-35 0.49019471430734995 1102 36-37 0.650439287577683 1102 38-39 0.5347111042551091 1102 40-41 0.5687739825233464 1102 42-43 0.7097339514923888 1102 44-45 0.8059130253184676 1102 46-47 0.8609749524398609 1102 48-49 0.9416392656487176 1102 50-51 0.9432370506084808 1102 52-53 0.9355311369365111 1102 54-55 1.251610277412503 1102 56-57 1.2985933436647343 1102 58-59 1.2389430245588713 1102 60-61 1.2554438798199001 1102 62-63 1.186583846475365 1102 64-65 1.1234528168633915 1102 66-67 1.2607379399965808 1102 68-69 1.2101845218318168 1102 70-71 1.4164538572236864 1102 72-73 1.5754281622416606 1102 74-75 1.3433859774164851 1102 76-77 1.3581833811299795 1102 78-79 1.384804534586376 1102 80-81 1.5323951147683346 1102 82-83 1.6099893970364931 1102 84-85 1.619258863658711 1102 86-87 1.7971376468845577 1102 88-89 1.8313514892797578 1102 90-91 1.6488854916444957 1102 92-93 1.8828459703702265 1102 94-95 1.775888816261503 1102 96-97 1.6554355539633114 1102 98-99 1.8056264246922922 1102 100-101 1.9283007912140633 1103 1 0.10350711765799403 1103 2 0.10819174976434454 1103 3 0.1365706647211553 1103 4 0.0647362081681635 1103 5 0.05045427501962507 1103 6 0.07399063356575653 1103 7 0.05292460152388401 1103 8 0.07415381919217623 1103 9 0.11112720631916773 1103 10-11 0.07989446422602242 1103 12-13 0.030281730966223108 1103 14-15 0.10727351710016819 1103 16-17 0.13899190759767777 1103 18-19 0.1232159904645087 1103 20-21 0.017900627763047794 1103 22-23 0.03957971936446825 1103 24-25 -0.048975856692656805 1103 26-27 -0.07724917622255845 1103 28-29 -0.14255047318324898 1103 30-31 -0.06871081605088847 1103 32-33 0.010765154239457786 1103 34-35 0.2104078977461512 1103 36-37 0.1974123312261682 1103 38-39 0.09638775823648871 1103 40-41 0.32074325084308697 1103 42-43 0.3665438468421982 1103 44-45 0.3935119987591378 1103 46-47 0.33573618066180444 1103 48-49 0.5116246227828967 1103 50-51 0.5768191613725477 1103 52-53 0.577452666946364 1103 54-55 0.6690863360518122 1103 56-57 0.7022840656166949 1103 58-59 0.6065613628915898 1103 60-61 0.784797920384392 1103 62-63 0.8703756518802024 1103 64-65 0.5533553689122641 1103 66-67 0.8480119876028596 1103 68-69 1.0698340970557645 1103 70-71 0.9942963450988493 1103 72-73 0.9759413731415663 1103 74-75 0.7999913586009058 1103 76-77 0.8806986211258163 1103 78-79 0.867016707603252 1103 80-81 0.8397998249665797 1103 82-83 1.026506609280574 1103 84-85 1.1007190654588541 1103 86-87 1.2493972739728498 1103 88-89 1.3240794542652452 1103 90-91 1.3939448997774733 1103 92-93 1.585044738333984 1103 94-95 1.6021368179962785 1103 96-97 1.8851009273449293 1103 98-99 1.9124479417280362 1103 100-101 1.628127543280641 1104 1 0.033469439133046563 1104 2 0.10947728584768512 1104 3 0.048305626381228706 1104 4 0.08364573561488697 1104 5 0.08204051389493117 1104 6 0.05777364850203526 1104 7 0.1381750733944216 1104 8 0.14793773022022094 1104 9 0.1431833955396158 1104 10-11 0.17524118773051356 1104 12-13 0.14451223705893312 1104 14-15 0.29040736333935513 1104 16-17 0.19491358064366437 1104 18-19 0.16081969886202785 1104 20-21 0.13530785026262748 1104 22-23 0.20764258233496946 1104 24-25 0.14561459381391728 1104 26-27 0.18916814935602844 1104 28-29 0.2822405454170607 1104 30-31 0.3626886659227395 1104 32-33 0.3005221142410548 1104 34-35 0.3917569567786856 1104 36-37 0.3790670571145469 1104 38-39 0.4085579644656079 1104 40-41 0.6763238400732021 1104 42-43 0.4610783982913844 1104 44-45 0.6843465446610892 1104 46-47 0.7291344024174862 1104 48-49 0.7197196028199571 1104 50-51 0.6650554678814515 1104 52-53 0.5633560056704638 1104 54-55 0.752452028254254 1104 56-57 0.9576983800425012 1104 58-59 1.1411786677036062 1104 60-61 1.19217247109394 1104 62-63 1.1936757444763586 1104 64-65 1.2341930866945674 1104 66-67 1.3360528607660456 1104 68-69 1.4257610328628765 1104 70-71 1.3106110468354188 1104 72-73 1.5755988669578223 1104 74-75 1.4257256915743781 1104 76-77 1.4053155418985028 1104 78-79 1.456408688008711 1104 80-81 1.5416779195056804 1104 82-83 1.666925894882084 1104 84-85 1.3920461773551125 1104 86-87 1.2284931600582532 1104 88-89 1.5549758447222928 1104 90-91 1.5557678348904744 1104 92-93 1.5911740059709913 1104 94-95 1.2457929861656751 1104 96-97 1.12884329100741 1104 98-99 1.2146831928399706 1104 100-101 1.1657969650738735 1105 1 -0.010776237670771849 1105 2 -0.10373744946007335 1105 3 -0.05798536944465127 1105 4 0.03805175164880836 1105 5 0.08155684596940205 1105 6 0.10361609201890332 1105 7 0.039662517905128425 1105 8 0.029091245094669205 1105 9 0.0957474981037123 1105 10-11 0.09536641134410218 1105 12-13 -0.07852824528387004 1105 14-15 -0.0030432429019526808 1105 16-17 0.13328567366691146 1105 18-19 0.13589754952127464 1105 20-21 0.041961597032660336 1105 22-23 0.18864536508193908 1105 24-25 0.1371653126902217 1105 26-27 0.11491187161137617 1105 28-29 -0.03689580918441493 1105 30-31 0.14995262934949238 1105 32-33 0.14870504672631313 1105 34-35 0.39485441917847197 1105 36-37 0.37680441880539917 1105 38-39 0.42097597281384935 1105 40-41 0.37949749673523314 1105 42-43 0.2795174097536446 1105 44-45 0.45801811426289163 1105 46-47 0.591600446278882 1105 48-49 0.6299147263871774 1105 50-51 0.6189049345216091 1105 52-53 0.4910012716878285 1105 54-55 0.7342565071517555 1105 56-57 0.7566250347831058 1105 58-59 0.6425750103790122 1105 60-61 0.8252540320824835 1105 62-63 0.5248822640549662 1105 64-65 0.6800040586683309 1105 66-67 0.762750667696416 1105 68-69 0.6259863028555905 1105 70-71 0.7896493427341795 1105 72-73 0.8845500363741081 1105 74-75 0.8612736277037101 1105 76-77 0.6056981696067112 1105 78-79 0.6821143131329421 1105 80-81 0.6570294069501941 1105 82-83 0.8214379203243425 1105 84-85 0.6159728320957214 1105 86-87 0.8507899201457114 1105 88-89 0.8678261726889005 1105 90-91 1.0520343160406753 1105 92-93 1.0535274148360259 1105 94-95 0.7564502444973549 1105 96-97 0.8640731989117363 1105 98-99 0.8852573000419852 1105 100-101 0.9394188427655195 1106 1 0.027109459243654044 1106 2 -0.6197543213251002 1106 3 -0.022018657178350054 1106 4 0.2043764210514496 1106 5 0.12046853467589358 1106 6 -0.017393939900841815 1106 7 0.09493307574066279 1106 8 0.1289164464930579 1106 9 0.21502854385534675 1106 10-11 -0.4220564492730148 1106 12-13 -0.26436575858376443 1106 14-15 -0.1951507060422486 1106 16-17 -0.17453086380078275 1106 18-19 -0.1661620340020562 1106 20-21 -0.19434901248246916 1106 22-23 -0.17790920248151565 1106 24-25 -0.25530504754689787 1106 26-27 -0.13686461417673712 1106 28-29 -0.04549324040496572 1106 30-31 0.023366142041389537 1106 32-33 0.06738569728110377 1106 34-35 -0.06881787144320128 1106 36-37 0.03342016587941288 1106 38-39 0.0754257372157312 1106 40-41 0.18356156422269265 1106 42-43 0.07661803294866587 1106 44-45 0.052827355788956254 1106 46-47 0.06306267458105452 1106 48-49 0.1964331942394395 1106 50-51 0.22359409700830923 1106 52-53 0.12369621012832255 1106 54-55 0.2882635750952076 1106 56-57 0.2727939054321382 1106 58-59 0.12485765211788191 1106 60-61 0.29371428440046543 1106 62-63 0.5423811240640859 1106 64-65 0.6387933883536903 1106 66-67 0.9424987297119145 1106 68-69 0.8710748421472765 1106 70-71 0.8861305708843545 1106 72-73 0.7795853760913865 1106 74-75 0.6915669853568502 1106 76-77 0.7054663714610996 1106 78-79 0.8996150731268635 1106 80-81 0.8470658866583562 1106 82-83 0.7822602337458342 1106 84-85 0.63091621254868 1106 86-87 0.6437960760964607 1106 88-89 1.0571691579450189 1106 90-91 0.8398155137910948 1106 92-93 0.961473151270134 1106 94-95 0.7445088400285869 1106 96-97 0.7737004218939525 1106 98-99 0.6022355642158708 1106 100-101 0.27639064699108573 1107 1 0.16628251977221353 1107 2 0.16037781848734767 1107 3 0.16433684154287675 1107 4 0.1463485722307638 1107 5 0.03367683248777098 1107 6 -0.013260663401837292 1107 7 0.09352500526609475 1107 8 -0.05344217811620666 1107 9 0.09946730486699806 1107 10-11 0.10763806621434213 1107 12-13 0.0929019311008048 1107 14-15 -0.015720470179786616 1107 16-17 0.04443048885622147 1107 18-19 0.1589814726759755 1107 20-21 0.07067016910321655 1107 22-23 0.16159278671995736 1107 24-25 0.14967008743462884 1107 26-27 -0.1073114740801202 1107 28-29 0.0594534774125961 1107 30-31 0.25131371237386446 1107 32-33 0.03869999111457645 1107 34-35 0.2382068514894513 1107 36-37 0.13192968562934482 1107 38-39 0.3303305397164422 1107 40-41 0.3311411322817932 1107 42-43 0.23120598407113846 1107 44-45 0.3858267874456729 1107 46-47 0.4958134560530638 1107 48-49 0.4739609009743049 1107 50-51 0.45708940817625177 1107 52-53 0.6157962385454852 1107 54-55 0.6396339928275196 1107 56-57 0.7877541214025854 1107 58-59 0.9144562596768466 1107 60-61 0.9346833096917351 1107 62-63 0.8623342357435391 1107 64-65 0.6337806006299047 1107 66-67 0.9013272321273718 1107 68-69 1.0341730234487834 1107 70-71 1.154372969292993 1107 72-73 1.2112683826273383 1107 74-75 1.178826149892231 1107 76-77 1.088058016814891 1107 78-79 0.7880883609927345 1107 80-81 0.9900155882781334 1107 82-83 1.0078697819129268 1107 84-85 0.9563048172659308 1107 86-87 0.8375975822061541 1107 88-89 0.8530323293005182 1107 90-91 0.819533473438721 1107 92-93 0.7912074507870415 1107 94-95 0.8669647934244402 1107 96-97 0.5058196318967951 1107 98-99 1.0289405940538927 1107 100-101 1.230643986007852 1108 1 0.0673496710840169 1108 2 0.22567629465119055 1108 3 0.16579264097435242 1108 4 0.15465278025536833 1108 5 0.1972501583650228 1108 6 0.21989931609394375 1108 7 0.14708668101985722 1108 8 0.09593197724984037 1108 9 0.04260351811085883 1108 10-11 0.09350320438480964 1108 12-13 0.21716913833320461 1108 14-15 0.27584996646684345 1108 16-17 0.280408400222079 1108 18-19 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-0.3413658259949166 2316 80-81 0.07851097065854162 2316 82-83 0.10990776053697715 2316 84-85 0.0883438335573068 2316 86-87 0.29532301543226325 2316 88-89 0.5826587690639329 2316 90-91 0.690954040005078 2316 92-93 0.7407425758259549 2316 94-95 0.7209359462728351 2316 96-97 0.660798359716658 2316 98-99 1.5020027503265894 2316 100-101 1.8328967617679446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4716.0 3 778.0 4 788.0 5 812.0 6 976.0 7 1212.0 8 1450.0 9 1833.0 10 2380.0 11 3132.0 12 4473.0 13 5822.0 14 6495.0 15 8637.0 16 11325.0 17 15239.0 18 12517.0 19 8585.0 20 8770.0 21 9292.0 22 9903.0 23 11049.0 24 12603.0 25 14292.0 26 16525.0 27 19201.0 28 22640.0 29 27198.0 30 32707.0 31 40911.0 32 51081.0 33 65939.0 34 87021.0 35 117830.0 36 161044.0 37 200347.0 38 136401.0 39 12210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.38644081422926 19.398416213278153 14.841053317790248 22.37408965470234 2 15.077171128538774 20.829086322835686 40.44376783416876 23.649974714456782 3 18.379770627337987 26.054260932146324 30.255408756197877 25.31055968431782 4 12.317383014644296 16.91164076017227 37.84589908214412 32.92507714303931 5 13.163427247802659 37.998719546653135 34.0386599086214 14.799193296922809 6 30.503298132876992 36.59657133876692 18.199407165526544 14.70072336282954 7 27.72796763257313 33.634638874264525 21.32721118191922 17.31018231124312 8 26.566843855212973 33.86155194814266 20.45244198879591 19.11916220784845 9 27.053532835306086 16.444242633286326 20.86055964756786 35.64166488383973 10-11 24.337766778459752 27.16104797613179 27.65317916046463 20.848006084943822 12-13 25.54455860529428 24.720172997856736 28.315432060226165 21.419836336622815 14-15 22.600643658410515 25.726211181425757 27.01754431918193 24.655600840981798 16-17 21.317779374518178 29.27525059172037 27.40034171796656 22.006628315794895 18-19 21.25791785681861 27.269465531570162 30.5787717631941 20.893844848417125 20-21 22.185007981443416 26.45247210859826 30.38919849022606 20.973321419732258 22-23 21.87341087013931 26.46184669711976 30.32857476467237 21.336167668068555 24-25 21.481974569279725 26.638701313440393 30.52411029581873 21.355213821461152 26-27 21.214353899492323 26.983813823395515 30.37875120742688 21.42308106968528 28-29 21.064030419578895 26.976159397196405 30.71814304361682 21.24166713960788 30-31 21.209473685129733 26.664605803077496 30.622293479200557 21.50362703259221 32-33 21.11909464460033 26.93896831675215 30.280657729067457 21.661279309580063 34-35 21.55732018722035 26.971403973608236 29.996992005895496 21.474283833275923 36-37 21.726084204552894 26.704891197590413 29.946264486980816 21.622760110875877 38-39 21.262608407205178 27.05101849305801 30.164111159122353 21.522261940614456 40-41 21.29296269252441 27.015589626933973 30.15935296872429 21.53209471181733 42-43 21.398427528551895 26.969823424342053 29.704755652324224 21.926993394781825 44-45 21.31963565191733 27.21170181004244 29.581762691859787 21.886899846180437 46-47 21.59611685747987 27.43602235452403 29.02704911377286 21.940811674223237 48-49 21.655470065171382 27.569833963160356 28.73491209198036 22.039783879687906 50-51 21.684893687717796 27.703674789250755 28.492311642108543 22.11911988092291 52-53 21.901641749720728 27.774541306639577 28.305618562418715 22.018198381220973 54-55 21.549956363791946 27.64265372758869 28.411646392601885 22.39574351601748 56-57 21.6125705362127 28.01699023087912 28.25594808656952 22.114491146338665 58-59 21.798569408059965 27.336080007017237 28.547645013155144 22.31770557176765 60-61 21.781185696922616 27.356959406824227 28.899644103264606 21.96221079298855 62-63 21.655319000357885 27.396321610321138 28.891856740077337 22.056502649243633 64-65 21.484724466078564 27.281816428257837 28.9231409741115 22.310318131552098 66-67 21.525318320185917 27.515782353005356 28.572070745092905 22.38682858171582 68-69 21.350859130975195 27.295143818407414 28.90687361515838 22.447123435459016 70-71 21.369088067483766 27.45128589864953 28.794597946909086 22.38502808695762 72-73 21.463098045428737 27.368274955448975 28.539149691885406 22.629477307236883 74-75 21.137448453338266 27.639093058091905 28.462704833601617 22.76075365496821 76-77 21.234932856184376 27.492711153909788 28.54150178821728 22.730854201688558 78-79 21.247921077329377 27.32771678634568 28.47694087347491 22.947421262850035 80-81 21.241630365514048 27.603076359001665 28.3034194376304 22.85187383785388 82-83 21.49299069499485 27.204832317871546 28.418629213875455 22.883547773258147 84-85 21.183813130001777 27.046541636901654 28.3175093309757 23.45213590212087 86-87 21.48014897325586 26.840454251472234 28.463204880122266 23.21619189514964 88-89 21.382547048962174 26.46953945504865 28.579939480886896 23.56797401510228 90-91 21.176261093405888 26.565253711331838 28.943631051672774 23.314854143589503 92-93 21.28706259460422 26.519865358662386 28.82934315209478 23.363728894638612 94-95 21.049167563566403 26.64254857443133 28.59456029217545 23.713723569826808 96-97 21.232850104908277 26.78637602251196 28.431604911112768 23.549168961466997 98-99 21.398424844900518 27.089865378932487 27.917033508821014 23.594676267345978 100-101 21.779774857223657 26.267399161889998 28.292802571065216 23.660023409821125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1905.0 1 1090.0 2 289.0 3 313.5 4 283.5 5 277.0 6 436.5 7 842.5 8 1365.0 9 1622.5 10 1582.0 11 1504.5 12 1564.5 13 1687.0 14 1808.0 15 1980.0 16 2099.0 17 2147.5 18 2292.0 19 2506.5 20 2717.5 21 3046.0 22 3535.0 23 4087.0 24 4715.0 25 5599.5 26 7011.0 27 8849.0 28 10219.5 29 11940.5 30 14255.5 31 16240.0 32 18542.5 33 21550.5 34 25088.5 35 27792.5 36 31129.5 37 34577.5 38 35777.5 39 35987.0 40 35624.0 41 36773.0 42 38952.0 43 40871.5 44 42443.0 45 43939.0 46 43700.0 47 42516.5 48 43703.0 49 42997.0 50 40262.0 51 37918.0 52 35862.5 53 35093.0 54 34111.5 55 31430.5 56 27902.5 57 24489.5 58 21789.0 59 19395.0 60 16650.0 61 13937.5 62 11467.0 63 8946.0 64 6716.0 65 5195.5 66 4010.0 67 3158.5 68 2389.5 69 1596.5 70 1176.5 71 908.0 72 730.5 73 601.0 74 458.0 75 356.5 76 260.5 77 168.5 78 106.0 79 73.0 80 48.0 81 32.0 82 19.0 83 15.0 84 10.0 85 5.0 86 6.0 87 5.0 88 2.5 89 2.5 90 2.0 91 2.0 92 2.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.329926646192866 2 0.27967118820625464 3 0.2774066441721959 4 0.27723244847726836 5 0.28106475376567547 6 0.242915896576532 7 0.24256750518667683 8 0.2471836911022581 9 0.2568515521707396 10-11 0.2623822654846908 12-13 0.25410796997563 14-15 0.24439655998341656 16-17 0.2390835912881249 18-19 0.22837055605007778 20-21 0.23207221456728921 22-23 0.2313754317875788 24-25 0.2324641548808763 26-27 0.22815281143141827 28-29 0.24552883200044595 30-31 0.2598128789845088 32-33 0.24909984374646166 34-35 0.24844660989048317 36-37 0.23594806877942817 38-39 0.2237543701344965 40-41 0.22057529870206788 42-43 0.23307383981312288 44-45 0.22958992591457095 46-47 0.24152233101711126 48-49 0.2478804738819685 50-51 0.26072740638287867 52-53 0.2784518183417615 54-55 0.24992727329736772 56-57 0.22074949439699545 58-59 0.2076848172774258 60-61 0.2122139053455433 62-63 0.21983496699862556 64-65 0.21016710593014404 66-67 0.21526233000677622 68-69 0.21517523215931245 70-71 0.21735267834590735 72-73 0.22101078793938686 74-75 0.22384146798196028 76-77 0.23686259617779806 78-79 0.23594806877942817 80-81 0.22854475174500535 82-83 0.21896398852398763 84-85 0.20498478400604808 86-87 0.2112122800997096 88-89 0.22170757071909727 90-91 0.21957367345623421 92-93 0.2240156636768879 94-95 0.21957367345623421 96-97 0.22728183295678028 98-99 0.2316367253299702 100-101 0.21434780260840633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1148134.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.73332726542742 #Duplication Level Percentage of deduplicated Percentage of total 1 75.16368766750394 38.13303964911848 2 13.071588190152452 13.263303230598009 3 4.651474592880924 7.079543483623461 4 2.217966369970097 4.5009925484562 5 1.1998579913620002 3.043639407390337 6 0.7417427196570227 2.257864568586475 7 0.521184806170038 1.8509007536035023 8 0.35972028252156346 1.4599845453742795 9 0.2719730692203767 1.2418288855326103 >10 1.596762338214288 15.264211482817155 >50 0.14029982719901307 4.927877685355058 >100 0.0620193852124096 5.1923405266951255 >500 8.613799679503794E-4 0.31988891767782107 >1k 6.891039743603035E-4 0.9102849938837448 >5k 1.7227599359007588E-4 0.5542993212876342 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6342 0.5523745486154056 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4338 0.3778304622979548 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3050 0.2656484347645832 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1978 0.172279542283392 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.7419569492759557E-4 0.0 0.0 0.0 0.0 8 1.7419569492759557E-4 0.0 0.0 0.0 0.0 9 1.7419569492759557E-4 0.0 0.0 0.0 0.0 10-11 1.7419569492759557E-4 0.0 0.0 0.0 0.0 12-13 1.7419569492759557E-4 0.0 0.0 0.0 0.0 14-15 2.6129354239139336E-4 0.0 0.0 0.0 0.0 16-17 2.6129354239139336E-4 0.0 0.0 0.0 0.0 18-19 3.0484246612329225E-4 0.0 0.0 0.0 0.0 20-21 3.4839138985519113E-4 0.0 0.0 4.354892373189889E-5 0.0 22-23 4.354892373189889E-4 0.0 0.0 4.790381610508878E-4 0.0 24-25 4.354892373189889E-4 0.0 0.0 7.8388062717418E-4 0.0 26-27 4.354892373189889E-4 0.0 0.0 0.00117582094076127 0.0 28-29 5.661360085146856E-4 0.0 0.0 0.0030484246612329227 0.0 30-31 6.096849322465845E-4 0.0 0.0 0.006706534254712428 0.0 32-33 6.096849322465845E-4 0.0 0.0 0.011671111560148903 0.0 34-35 6.532338559784834E-4 0.0 0.0 0.01685343348424487 0.0 36-37 6.967827797103823E-4 0.0 0.0 0.023647065586421098 0.0 38-39 8.709784746379778E-4 0.0 0.0 0.033576220197294046 0.0 40-41 9.145273983698766E-4 0.0 0.0 0.05077804507139411 0.0 42-43 0.0010016252458336745 0.0 0.0 0.0746428552764747 0.0 44-45 0.001219369864493169 0.0 0.0 0.09602537682883705 0.0 46-47 0.0014371144831526635 0.0 0.0 0.13879041993356178 0.0 48-49 0.0014806634068845622 0.0 0.0 0.19501208047144325 0.0 50-51 0.0014806634068845622 0.0 0.0 0.22876249636366486 0.0 52-53 0.0014806634068845622 0.0 0.0 0.2906890659104251 0.0 54-55 0.0014806634068845622 0.0 0.0 0.3928983899091918 0.0 56-57 0.0014806634068845622 0.0 0.0 0.546930933148918 0.0 58-59 0.0014806634068845622 0.0 0.0 0.7450785361290582 0.0 60-61 0.0014806634068845622 0.0 0.0 0.9821588769255156 0.0 62-63 0.0014806634068845622 0.0 0.0 1.231389367443173 0.0 64-65 0.0014806634068845622 0.0 0.0 1.4980829763773218 0.0 66-67 0.00156776125434836 0.0 0.0 1.8322338681721821 0.0 68-69 0.0016548591018121577 0.0 0.0 2.251740650481564 0.0 70-71 0.0016984080255440567 0.0 0.0 2.5697348915718896 0.0 72-73 0.0017419569492759556 0.0 0.0 2.9314522520890423 0.0 74-75 0.0017419569492759556 0.0 0.0 3.3339314052192517 0.0 76-77 0.0017419569492759556 0.0 0.0 3.774254573072481 0.0 78-79 0.0017419569492759556 0.0 0.0 4.28177373024403 0.0 80-81 0.00195970156793545 0.0 0.0 4.955214286834115 0.0 82-83 0.002003250491667349 0.0 0.0 5.612890133033252 0.0 84-85 0.002046799415399248 0.0 0.0 6.471544262255103 0.0 86-87 0.002090348339131147 0.0 0.0 7.456925759536778 0.0 88-89 0.002090348339131147 0.0 0.0 8.72659463094029 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3100 0.0 48.76762 1 GTATCAA 5945 0.0 45.181717 1 ATCAACG 7495 0.0 35.81446 3 TATCAAC 7595 0.0 35.468018 2 TCAACGC 7630 0.0 35.05778 4 CAACGCA 7760 0.0 34.412254 5 AACGCAG 7990 0.0 33.46944 6 ACGCAGA 9125 0.0 29.046122 7 CGCAGAG 9175 0.0 28.732521 8 GTATAGA 220 1.8189894E-12 28.092268 1 GCAGAGT 9540 0.0 27.2349 9 GTACATG 6260 0.0 27.111904 1 TACATGG 6475 0.0 25.460758 2 ACATGGG 6515 0.0 24.502277 3 GAGTACT 4970 0.0 24.46535 12-13 AGTACTT 5275 0.0 23.455957 12-13 GTACTTT 5390 0.0 22.823322 14-15 AGAGTAC 8630 0.0 21.986917 10-11 CATGGGA 4380 0.0 21.477892 4 CAGAGTA 9325 0.0 21.035828 10-11 >>END_MODULE