FastQCFastQC Report
Thu 26 May 2016
SRR937483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148134
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75950.661508151487544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57650.5021190906287942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39600.3449074759566392No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14330.1248112154156222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38050.046.9592971
GTATCAA66750.039.868061
CGCTATA753.0703584E-431.6808721
ATCAACG87400.030.0534233
TATCAAC88550.029.7704032
TCAACGC88550.029.6631224
CAACGCA90850.028.9121555
AACGCAG93300.028.2026216
GTAAGGT2850.024.9992313
ACGCAGA107450.024.3592137
CGCAGAG108050.024.0920588
GTACATG64950.023.5594481
GCAGAGT111750.022.5292369
AGTACTT61700.022.44251312-13
TAAGGTG3600.022.4298675
GAGTACT61050.022.13679112-13
TACATGG68900.021.9913432
ACATGGG67600.021.6413463
GTACTTT64000.021.03945214-15
AGAGTAC102350.020.72295610-11
Produced by FastQC (version 0.11.5)