##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937483_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1148134 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.134929372355494 33.0 31.0 34.0 31.0 34.0 2 32.34403911041743 34.0 31.0 34.0 31.0 34.0 3 32.4020227604095 34.0 31.0 34.0 31.0 34.0 4 36.062047635554734 37.0 35.0 37.0 35.0 37.0 5 35.979109581285805 37.0 35.0 37.0 35.0 37.0 6 36.01848477616724 37.0 35.0 37.0 35.0 37.0 7 35.95022270919596 37.0 35.0 37.0 35.0 37.0 8 35.92261182057147 37.0 35.0 37.0 35.0 37.0 9 37.597185520157055 39.0 37.0 39.0 35.0 39.0 10-11 37.520657867461466 39.0 37.0 39.0 35.0 39.0 12-13 37.40356874720198 39.0 37.0 39.0 34.0 39.0 14-15 38.588295007377184 40.0 38.0 41.0 34.0 41.0 16-17 38.547901203169666 40.0 38.0 41.0 34.0 41.0 18-19 38.561981005701426 40.0 38.0 41.0 34.0 41.0 20-21 38.46823889894385 40.0 38.0 41.0 34.0 41.0 22-23 38.39903878815539 40.0 38.0 41.0 34.0 41.0 24-25 38.4065096060216 40.0 38.0 41.0 34.0 41.0 26-27 38.23302767795397 40.0 38.0 41.0 34.0 41.0 28-29 38.07341259818105 40.0 37.5 41.0 33.0 41.0 30-31 38.09571748593805 40.0 37.0 41.0 34.0 41.0 32-33 37.96566602852977 40.0 37.0 41.0 33.0 41.0 34-35 37.80676079621368 40.0 37.0 41.0 33.0 41.0 36-37 37.62473195637443 40.0 37.0 41.0 33.0 41.0 38-39 37.48220634525238 40.0 36.0 41.0 33.0 41.0 40-41 37.26089506973925 40.0 36.0 41.0 32.0 41.0 42-43 37.003820547079 40.0 36.0 41.0 31.5 41.0 44-45 36.62646825196362 39.0 35.5 41.0 31.0 41.0 46-47 36.159837179283954 39.0 35.0 41.0 30.0 41.0 48-49 35.7539337742807 39.0 35.0 41.0 28.5 41.0 50-51 35.48031762843013 39.0 35.0 40.0 27.5 41.0 52-53 35.217124046496316 39.0 35.0 40.0 27.0 41.0 54-55 34.982462848413164 38.0 35.0 40.0 26.0 41.0 56-57 34.676714564676246 38.0 34.0 40.0 26.0 41.0 58-59 34.40451375884697 38.0 34.0 40.0 25.0 41.0 60-61 34.04598026014385 37.5 34.0 40.0 24.0 41.0 62-63 33.66798953780656 37.0 33.0 40.0 23.0 41.0 64-65 33.25288947108961 36.5 33.0 39.0 22.0 41.0 66-67 32.821306136740134 36.0 32.0 39.0 20.5 40.0 68-69 32.35028271961286 35.0 32.0 39.0 19.0 40.0 70-71 31.908444049213767 35.0 31.0 38.0 18.0 40.0 72-73 31.427637801859362 35.0 31.0 37.0 14.5 39.5 74-75 30.95080495830626 35.0 30.5 37.0 10.0 39.0 76-77 29.63562354219978 33.5 28.5 35.5 8.0 37.5 78-79 30.289992718619953 34.0 30.0 36.0 7.0 38.0 80-81 30.115531375257593 34.0 30.0 35.5 6.0 37.0 82-83 29.725849073366 34.0 30.0 35.0 2.0 37.0 84-85 29.319065109124892 34.0 29.0 35.0 2.0 36.0 86-87 28.964967939282346 34.0 29.0 35.0 2.0 36.0 88-89 28.545648417345014 34.0 29.0 35.0 2.0 35.5 90-91 28.00890183550004 33.0 27.5 35.0 2.0 35.0 92-93 27.51050182295795 33.0 26.5 35.0 2.0 35.0 94-95 27.047851557396612 33.0 26.0 35.0 2.0 35.0 96-97 26.391537921531807 32.5 25.0 34.5 2.0 35.0 98-99 25.434955327514036 32.0 22.0 34.0 2.0 35.0 100-101 22.596179975508086 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11698746993745601 1101 2 0.16602981997909438 1101 3 0.09455367882844712 1101 4 0.07858624915267853 1101 5 0.04924743218747096 1101 6 0.08583101379800695 1101 7 0.054483297427545097 1101 8 0.07112969398014712 1101 9 0.2290112096726702 1101 10-11 0.06374821876283221 1101 12-13 0.12056915881095165 1101 14-15 0.1507707793732962 1101 16-17 0.08181764326780439 1101 18-19 0.029148896474126218 1101 20-21 0.082663359676026 1101 22-23 0.08118084838456952 1101 24-25 0.010657031095462344 1101 26-27 0.05984502526582958 1101 28-29 -0.10182700314343407 1101 30-31 0.13669597305815984 1101 32-33 0.18484572875077987 1101 34-35 0.2121883151647168 1101 36-37 0.33845860643289427 1101 38-39 0.375293355538453 1101 40-41 0.3895404479181863 1101 42-43 0.509741217522766 1101 44-45 0.5682896009692584 1101 46-47 0.6659493833523626 1101 48-49 0.521262522107989 1101 50-51 0.5029939145334765 1101 52-53 0.5985686958323058 1101 54-55 0.5823376734456005 1101 56-57 0.7115575077508325 1101 58-59 0.6540410238827619 1101 60-61 0.4703278569079785 1101 62-63 0.5042052661293752 1101 64-65 0.49466344301979603 1101 66-67 0.5389551526803373 1101 68-69 0.5537615625929355 1101 70-71 0.7343107687496406 1101 72-73 0.6736769094533805 1101 74-75 0.6873207027101778 1101 76-77 0.7576251824263842 1101 78-79 0.7890267076980599 1101 80-81 0.7158431325912922 1101 82-83 0.6063074433224038 1101 84-85 0.5116241493821505 1101 86-87 0.6389067246380762 1101 88-89 0.8248877420199356 1101 90-91 1.0212511348172981 1101 92-93 0.9526680020514604 1101 94-95 0.9044413591687679 1101 96-97 1.0817501078363954 1101 98-99 1.46320492570241 1101 100-101 2.0593691322915966 1102 1 0.22432100647957753 1102 2 0.19151565191776143 1102 3 0.23378370925225056 1102 4 0.13310965746238423 1102 5 0.11048516537434239 1102 6 0.12600404799268006 1102 7 0.13187708729049774 1102 8 0.19176861306279136 1102 9 0.27934334100689284 1102 10-11 0.23674349703425435 1102 12-13 0.18166435829104444 1102 14-15 0.27420333993813983 1102 16-17 0.30063420133279806 1102 18-19 0.35675231728308887 1102 20-21 0.29489710345275455 1102 22-23 0.242952579742564 1102 24-25 0.10398028067226761 1102 26-27 0.11099296317596696 1102 28-29 0.1633383148687244 1102 30-31 0.18495259507640327 1102 32-33 0.3221893682200587 1102 34-35 0.38389225914043834 1102 36-37 0.43906554888837945 1102 38-39 0.3562040951225711 1102 40-41 0.37646434622944724 1102 42-43 0.521638516571322 1102 44-45 0.7048465732350948 1102 46-47 0.6503966267102115 1102 48-49 0.6491821626201499 1102 50-51 0.6964532756527362 1102 52-53 0.7287439538211871 1102 54-55 0.7801943959180022 1102 56-57 0.875184381920505 1102 58-59 0.9000292799488321 1102 60-61 0.9687319011461426 1102 62-63 0.8834350281642216 1102 64-65 0.9223954029684904 1102 66-67 1.0971968982821139 1102 68-69 1.0302038177503086 1102 70-71 0.8973162266307817 1102 72-73 1.069255872360113 1102 74-75 0.9682172560208535 1102 76-77 0.9477917944567267 1102 78-79 1.0632610802717757 1102 80-81 1.1050369305059142 1102 82-83 1.2262359943479346 1102 84-85 1.1985805419278392 1102 86-87 1.0883450248924689 1102 88-89 1.1089247336864574 1102 90-91 1.4055758777869443 1102 92-93 1.4562288525377198 1102 94-95 1.4656446810572277 1102 96-97 1.4689174732728851 1102 98-99 1.8774874357526166 1102 100-101 2.7044523424535676 1103 1 0.17872159109393237 1103 2 0.13463265685113157 1103 3 0.08357995152795894 1103 4 0.07842698908662271 1103 5 0.0884151941145177 1103 6 -0.04138421763459377 1103 7 -0.00933121045571994 1103 8 -0.008797388256624572 1103 9 0.11767674998433364 1103 10-11 0.027840597939366774 1103 12-13 0.16164720770837704 1103 14-15 0.14020312355014397 1103 16-17 0.17794954145392694 1103 18-19 0.08710444748997759 1103 20-21 0.07711885805684915 1103 22-23 0.05490533472395498 1103 24-25 0.061539579923575616 1103 26-27 0.03602449301618549 1103 28-29 0.11225000243378958 1103 30-31 0.0980163098806841 1103 32-33 0.18211641979146265 1103 34-35 0.24789904968923793 1103 36-37 0.19281944513308247 1103 38-39 0.22486127922703503 1103 40-41 0.1731575066724318 1103 42-43 0.1434211777494383 1103 44-45 0.3538786495467079 1103 46-47 0.41783866842266804 1103 48-49 0.47046940072534227 1103 50-51 0.5673627058141975 1103 52-53 0.583240331385241 1103 54-55 0.6243663840331664 1103 56-57 0.679126196471401 1103 58-59 0.6438336835362932 1103 60-61 0.6201328988231793 1103 62-63 0.6785710660724646 1103 64-65 0.7679971793672564 1103 66-67 0.6665481056308735 1103 68-69 0.6895209714962824 1103 70-71 0.814463921067091 1103 72-73 0.5831832136713793 1103 74-75 0.7403603499199498 1103 76-77 0.6609837145655426 1103 78-79 0.7774202134727979 1103 80-81 0.7455757778822303 1103 82-83 0.6281930908427746 1103 84-85 0.7153378898361922 1103 86-87 0.6970709396933614 1103 88-89 0.8825741768770463 1103 90-91 0.9890706998330323 1103 92-93 1.0454129139112283 1103 94-95 1.0536425052575673 1103 96-97 1.2622137547459893 1103 98-99 1.493090890075269 1103 100-101 2.115148511239873 1104 1 0.06413876447915356 1104 2 0.09698997557390499 1104 3 0.04620865804083962 1104 4 0.0377057347857388 1104 5 0.024923252539700513 1104 6 -0.026558877297517824 1104 7 0.015148025106888952 1104 8 -0.07438476218148793 1104 9 0.06458032624387755 1104 10-11 -0.08221767647237499 1104 12-13 -0.06632721242780093 1104 14-15 0.02525346826099195 1104 16-17 -0.03360563290704022 1104 18-19 -0.13513626551458913 1104 20-21 -0.07926408889025538 1104 22-23 -0.1630762399226171 1104 24-25 -0.13838745260455454 1104 26-27 -0.11689783289665456 1104 28-29 -0.14994957569189182 1104 30-31 0.0018970983845392198 1104 32-33 0.11515162481867947 1104 34-35 0.056548895433436996 1104 36-37 0.17134169025543144 1104 38-39 0.09508827491584526 1104 40-41 0.1417795024536943 1104 42-43 0.1443057483294652 1104 44-45 0.27025237954999426 1104 46-47 0.5759411158910623 1104 48-49 0.5469606467622725 1104 50-51 0.46342132443896134 1104 52-53 0.44865047827317284 1104 54-55 0.613045046950468 1104 56-57 0.5652258219619455 1104 58-59 0.5073566327307262 1104 60-61 0.5702552935786258 1104 62-63 0.4523926698491394 1104 64-65 0.23415990109662488 1104 66-67 0.41590142607755354 1104 68-69 0.6225456510012357 1104 70-71 0.4415581799636499 1104 72-73 0.6161478692521065 1104 74-75 0.6562267213030459 1104 76-77 0.7288871300975153 1104 78-79 0.7300136879334751 1104 80-81 0.7580648789884066 1104 82-83 0.5821718093746604 1104 84-85 0.6733904121922549 1104 86-87 0.8509159556452168 1104 88-89 1.1433152589860747 1104 90-91 1.2017779921708787 1104 92-93 1.1524580442214578 1104 94-95 1.0798061088550206 1104 96-97 1.155277577814804 1104 98-99 1.3797689925796313 1104 100-101 2.0367002292469074 1105 1 -0.07413394472379053 1105 2 -0.018026919675548925 1105 3 -0.02738506090172166 1105 4 -0.04925842078306886 1105 5 0.017499281162237423 1105 6 -0.1205097154344017 1105 7 -0.07550327019556846 1105 8 -0.013038442695638253 1105 9 0.06258271145556193 1105 10-11 -0.003668017690159786 1105 12-13 0.018072840576905946 1105 14-15 -0.05212014864751069 1105 16-17 0.06713643295130822 1105 18-19 0.022917136724856846 1105 20-21 -0.05675365358912643 1105 22-23 -0.020917623345397374 1105 24-25 -0.12485137627778187 1105 26-27 -0.15230331888353277 1105 28-29 -0.14285522731847067 1105 30-31 -0.15960823521381684 1105 32-33 -0.1005742083490162 1105 34-35 -0.10584923615073905 1105 36-37 -0.010696340619801958 1105 38-39 -0.14385196228021613 1105 40-41 -0.1254122420636392 1105 42-43 -0.05429790899511744 1105 44-45 0.08445929666388707 1105 46-47 0.15102393574132833 1105 48-49 0.3351886332460765 1105 50-51 0.385118468810532 1105 52-53 0.19108405675326168 1105 54-55 0.23843430024670198 1105 56-57 0.28659499972647495 1105 58-59 0.17814607154342355 1105 60-61 0.3797928275347644 1105 62-63 0.47417594686497466 1105 64-65 0.4221791815577731 1105 66-67 0.4474408814542201 1105 68-69 0.35191191350703477 1105 70-71 0.287670616192365 1105 72-73 0.3714052733467206 1105 74-75 0.5022414301084517 1105 76-77 0.52523643109798 1105 78-79 0.48700699608957265 1105 80-81 0.710203287522166 1105 82-83 0.6400811050746533 1105 84-85 0.6578401571071204 1105 86-87 0.6751672641988513 1105 88-89 0.8245134965796588 1105 90-91 0.9034857374832725 1105 92-93 0.9606787425584358 1105 94-95 0.9480960445868156 1105 96-97 1.1035433301503268 1105 98-99 1.3660490561852718 1105 100-101 1.8990569509059547 1106 1 -0.07580593134789737 1106 2 0.029757698047504277 1106 3 0.012837617316854733 1106 4 0.052105768303420064 1106 5 -0.05006417833008214 1106 6 -0.0071186225631478806 1106 7 -0.12062790919845412 1106 8 -0.02945431136868848 1106 9 -0.051093379135714656 1106 10-11 -0.1682827007726928 1106 12-13 -0.3292063811893229 1106 14-15 -0.16770572396290362 1106 16-17 -0.16999117002787045 1106 18-19 -0.22078441366273438 1106 20-21 -0.028861876723340174 1106 22-23 -0.08446261498546903 1106 24-25 -0.0687733249021889 1106 26-27 0.04926606856136573 1106 28-29 0.008545061479217964 1106 30-31 -0.05185059727491392 1106 32-33 0.03760218623983036 1106 34-35 -0.37603657465203355 1106 36-37 0.009367498869480073 1106 38-39 -0.003870582131249023 1106 40-41 -0.109575868754618 1106 42-43 -0.11980938490331283 1106 44-45 0.06394896074657908 1106 46-47 0.38835103712451513 1106 48-49 0.5203706517899249 1106 50-51 0.4503829466947096 1106 52-53 0.2599585057576732 1106 54-55 0.1921886702117419 1106 56-57 0.30340036528355085 1106 58-59 0.2281061718626205 1106 60-61 0.38405829341103725 1106 62-63 0.3549044010373379 1106 64-65 0.2958491921976858 1106 66-67 0.21169076745513138 1106 68-69 0.3546953912392077 1106 70-71 0.4864090262850844 1106 72-73 0.5343479698051503 1106 74-75 0.43740444880430474 1106 76-77 0.28114538382635956 1106 78-79 0.3236558028991148 1106 80-81 0.3189204177851188 1106 82-83 0.3599663459927278 1106 84-85 0.4033656929028311 1106 86-87 0.44311262063600054 1106 88-89 0.5819234373001336 1106 90-91 0.44918037192619664 1106 92-93 0.36407401539625184 1106 94-95 -0.24236793170137005 1106 96-97 0.09492689908177354 1106 98-99 0.4065852318958676 1106 100-101 1.016267013225459 1107 1 -0.1109039705635837 1107 2 -0.1098401990823561 1107 3 -0.08392140513270618 1107 4 -0.0054801936175863375 1107 5 -0.05839893252161232 1107 6 -0.03662700562652077 1107 7 -0.06394277137520987 1107 8 -0.12764129630758703 1107 9 -0.0936210858705806 1107 10-11 -0.05451174311426854 1107 12-13 0.007048236599828783 1107 14-15 -0.019459773408769365 1107 16-17 -0.010121178553013976 1107 18-19 -0.03941967936322044 1107 20-21 -0.06489768234993676 1107 22-23 -0.11347014553391688 1107 24-25 0.022850005589425848 1107 26-27 0.028066864241957035 1107 28-29 0.02124824420157978 1107 30-31 0.12310606655002942 1107 32-33 0.04797089894230311 1107 34-35 -0.019381985280048752 1107 36-37 0.005664742404576373 1107 38-39 -0.13129101407214705 1107 40-41 -0.11511508444089458 1107 42-43 -0.08386593505960604 1107 44-45 0.053477946253821074 1107 46-47 0.06628795102051299 1107 48-49 0.16436681547278909 1107 50-51 0.2928069307810901 1107 52-53 0.3840747319582931 1107 54-55 0.3176703411409818 1107 56-57 0.3297693621576627 1107 58-59 0.5149795733971487 1107 60-61 0.6745349913365857 1107 62-63 0.6360133808611508 1107 64-65 0.734189629822005 1107 66-67 0.7238575762076138 1107 68-69 0.6767051951607819 1107 70-71 0.7512101061373109 1107 72-73 0.7525804222064032 1107 74-75 0.6591215559370127 1107 76-77 0.49995612551433766 1107 78-79 0.5444791902250614 1107 80-81 0.8047303069581808 1107 82-83 0.7024443226906527 1107 84-85 0.5998242038434398 1107 86-87 0.8271911940943681 1107 88-89 0.9018424483828262 1107 90-91 0.8279925344723758 1107 92-93 0.9348945610058017 1107 94-95 1.172111323768931 1107 96-97 1.0910003574506284 1107 98-99 1.198795376823405 1107 100-101 1.7444866779029233 1108 1 0.03520535419525572 1108 2 0.07198654028108109 1108 3 -0.06407798279242627 1108 4 -0.05745279252712976 1108 5 0.01235832550531768 1108 6 0.10798551126615763 1108 7 0.06345831907634647 1108 8 0.064875596371472 1108 9 0.167485209856153 1108 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-0.10001900724694579 2316 96-97 -0.14776793143595413 2316 98-99 0.188854523860865 2316 100-101 0.908589614501448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 7.0 5 40.0 6 83.0 7 163.0 8 339.0 9 667.0 10 1124.0 11 1998.0 12 3103.0 13 4404.0 14 5728.0 15 7956.0 16 11263.0 17 15664.0 18 13411.0 19 7578.0 20 6637.0 21 6876.0 22 7096.0 23 7601.0 24 8863.0 25 10312.0 26 12261.0 27 15138.0 28 18436.0 29 22792.0 30 28149.0 31 35917.0 32 44848.0 33 59079.0 34 79638.0 35 113955.0 36 169494.0 37 242387.0 38 173031.0 39 12086.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.79459202292012 20.211774096012856 15.265179438115503 21.728454442951524 2 15.63998627337924 21.534942785424 39.389827319807615 23.43524362138914 3 18.839003112877066 26.23369746040096 30.529450395162932 24.397849031559034 4 12.952059602798977 17.605958886332083 37.255146176317396 32.186835334551546 5 13.270692015844979 38.455079764903445 33.597938898585866 14.676289320665711 6 30.80241504911448 37.054646931455736 17.510586743359223 14.632351276070562 7 27.825410622801865 34.17458240937034 20.874566905953486 17.12544006187431 8 27.052068835170807 34.271609411445006 19.869283550526333 18.807038202857854 9 27.23070652031906 16.53143274217121 20.697061492822264 35.54079924468746 10-11 24.7662729263309 27.44191880041877 27.043271952576966 20.748536320673374 12-13 25.8823934187908 25.04920271781049 28.140209127338245 20.928194736060462 14-15 22.81693600224364 25.8753769159349 27.062999615027515 24.244687466793945 16-17 21.36566811887811 29.43659015411093 27.50018726037205 21.69755446663891 18-19 21.389358733388264 27.134594045642757 30.94216354537014 20.53388367559884 20-21 22.228565854304335 26.32543442266706 30.990225000065326 20.45577472296328 22-23 21.938686856149932 26.31165228449055 31.01358957776718 20.736071281592338 24-25 21.74411773543756 26.521219004584395 31.05766881638764 20.676994443590402 26-27 21.509350641884918 26.838140828828077 30.932677643033767 20.719830886253238 28-29 21.064789107789352 26.922622990515087 31.280569887373467 20.732018014322094 30-31 21.402846367571616 26.550782576885894 31.128879133895985 20.9174919216465 32-33 21.2189109000145 26.780294765887007 30.97741852740736 21.02337580669113 34-35 21.65762010357676 26.707553299527753 30.694152424716975 20.940674172178507 36-37 21.915255536374676 26.508229875606855 30.574654177996646 21.001860410021827 38-39 21.63288431489704 26.819474033518738 30.61267238841459 20.93496926316963 40-41 21.479548554437024 26.89524916081224 30.644027613501564 20.981174671249175 42-43 21.566830673038773 26.88757948981054 30.173180101992596 21.37240973515809 44-45 21.526320098525083 27.17661875704404 29.963749875885565 21.33331126854531 46-47 21.80620903135 27.426720226036334 29.287086698939323 21.479984043674342 48-49 22.127687186338875 27.576528523674064 28.818979317745143 21.47680497224192 50-51 22.012195440601882 27.71523184576016 28.549934066929474 21.722638646708486 52-53 22.197191268614986 27.997341773695407 28.301139065649135 21.504327892040475 54-55 21.904542501136625 27.87884515222091 28.374954491374698 21.841657855267766 56-57 21.924575006053303 28.202021715235333 28.214302511727723 21.659100766983645 58-59 22.050997531647003 27.525097244746693 28.541964613886535 21.88194060971977 60-61 22.10229816380318 27.610670879879873 28.84097152422975 21.446059432087196 62-63 21.84039493647954 27.574917213495986 29.046391797473113 21.538296052551356 64-65 21.725817718140913 27.373546990159685 28.99317501267274 21.907460279026665 66-67 21.74889864771882 27.67525393377428 28.57667310610086 21.99917431240604 68-69 21.64590544309288 27.458380293589425 28.822332584872495 22.0733816784452 70-71 21.637500500812624 27.783516558171783 28.617304251942716 21.961678689072876 72-73 21.712016437141564 27.622053745585763 28.37140170790541 22.294528109367263 74-75 21.488737377344457 27.817049229445345 28.18612635807319 22.508087035137013 76-77 21.515084476202258 27.719325444590964 28.487963948458976 22.277626130747805 78-79 21.44958689490948 27.5541879257996 28.287856643910903 22.708368535380018 80-81 21.423544638517804 27.87361928137308 28.102468875584208 22.600367204524908 82-83 21.547933119130132 27.705854122918026 28.09274377226989 22.65346898568195 84-85 21.230884199927882 27.458380293589425 27.995077229661348 23.315658276821345 86-87 21.227487383876795 27.142781243304352 28.224754253423377 23.404977119395472 88-89 21.245865029691657 26.832146770324716 28.311242415954936 23.610745784028694 90-91 21.12878119400671 27.018894319509208 28.5821551423019 23.270169344182186 92-93 20.922831169939702 26.82888272034519 28.57338829859971 23.674897811115397 94-95 20.582310078788712 27.063435104264833 28.41323399533504 23.941020821611414 96-97 20.89825752046364 27.213896635758545 28.130253088925162 23.75759275485266 98-99 20.761497475247847 27.509063631593044 27.707516336311354 24.02192255684775 100-101 20.528416510086874 27.178593299343106 27.86349378079874 24.429496409771275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 358.0 1 274.0 2 230.0 3 283.0 4 270.5 5 275.0 6 456.0 7 924.5 8 1462.0 9 1777.5 10 1758.5 11 1698.0 12 1786.5 13 1956.5 14 2148.5 15 2268.5 16 2427.5 17 2605.5 18 2719.5 19 2813.5 20 3036.5 21 3427.0 22 3913.5 23 4464.0 24 5018.0 25 5990.0 26 7497.5 27 9128.0 28 10370.5 29 12147.0 30 14468.5 31 16199.0 32 18250.5 33 21225.0 34 24750.0 35 27471.0 36 31082.5 37 34362.0 38 35358.5 39 35679.5 40 35480.0 41 36632.5 42 38613.0 43 40723.5 44 42708.5 45 44321.0 46 43648.5 47 42252.0 48 43535.0 49 42923.5 50 40385.5 51 38040.5 52 35834.5 53 34630.0 54 33383.5 55 30995.0 56 27667.0 57 24402.0 58 22087.0 59 19788.5 60 16904.0 61 13958.5 62 11396.0 63 8904.0 64 6507.0 65 4919.0 66 3708.0 67 3052.0 68 2355.5 69 1465.5 70 1041.5 71 850.5 72 730.0 73 612.0 74 502.0 75 367.5 76 230.0 77 158.0 78 109.0 79 72.5 80 39.5 81 21.0 82 11.0 83 3.5 84 3.5 85 4.5 86 3.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0432005323420437 2 0.0 3 0.0 4 0.0 5 8.709784746379779E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006750083178444328 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.709784746379777E-5 22-23 0.0010016252458336745 24-25 4.3548923731898885E-5 26-27 0.002700033271377731 28-29 0.004137147754530395 30-31 3.483913898551911E-4 32-33 2.1774461865949448E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009537214297285856 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.1774461865949448E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.6129354239139336E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010190448153264342 92-93 0.0013064677119569666 94-95 0.0 96-97 0.0 98-99 1.3064677119569668E-4 100-101 0.01367436205181625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1148134.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.80408087200561 #Duplication Level Percentage of deduplicated Percentage of total 1 73.90981468560116 36.071005731510276 2 13.548749580809877 13.224685405127948 3 4.972467913235961 7.280301785130624 4 2.306588161153908 4.502836606214642 5 1.2505484315126574 3.0515933392951755 6 0.820874650246261 2.403721968983871 7 0.5577907115306321 1.9055724096636166 8 0.40719216165344796 1.5898111350225328 9 0.2872600701467274 1.2617517325265 >10 1.717699222381168 15.705667471607331 >50 0.14957242747083133 5.051632987278021 >100 0.0691143012908459 5.769766905294933 >500 0.0014324202871045743 0.448721689587413 >1k 5.371576076642154E-4 0.5654679753301167 >5k 3.5810507177614357E-4 1.1674628574270376 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7595 0.661508151487544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5765 0.5021190906287942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3960 0.3449074759566392 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1433 0.1248112154156222 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.709784746379778E-5 0.0 0.0 0.0 0.0 8 8.709784746379778E-5 0.0 0.0 0.0 0.0 9 8.709784746379778E-5 0.0 0.0 0.0 0.0 10-11 8.709784746379778E-5 0.0 0.0 0.0 0.0 12-13 8.709784746379778E-5 0.0 0.0 0.0 0.0 14-15 1.7419569492759557E-4 0.0 0.0 0.0 0.0 16-17 1.7419569492759557E-4 0.0 0.0 0.0 0.0 18-19 3.4839138985519113E-4 0.0 0.0 0.0 0.0 20-21 4.354892373189889E-4 0.0 0.0 4.354892373189889E-5 0.0 22-23 6.096849322465845E-4 0.0 0.0 4.790381610508878E-4 0.0 24-25 6.967827797103823E-4 0.0 0.0 7.8388062717418E-4 0.0 26-27 6.967827797103823E-4 0.0 0.0 0.00117582094076127 0.0 28-29 8.709784746379778E-4 0.0 0.0 0.00313552250869672 0.0 30-31 8.709784746379778E-4 0.0 0.0 0.006793632102176227 0.0 32-33 8.709784746379778E-4 0.0 0.0 0.011845307255076497 0.0 34-35 9.145273983698766E-4 0.0 0.0 0.017158275950368163 0.0 36-37 9.580763221017756E-4 0.0 0.0 0.02395190805254439 0.0 38-39 0.0010451741695655734 0.0 0.0 0.03379396481595354 0.0 40-41 0.0010451741695655734 0.0 0.0 0.0510393386137855 0.0 42-43 0.0010887230932974724 0.0 0.0 0.07525254020872128 0.0 44-45 0.001219369864493169 0.0 0.0 0.09693990422720693 0.0 46-47 0.001219369864493169 0.0 0.0 0.1401839854929825 0.0 48-49 0.001219369864493169 0.0 0.0 0.19705887988684248 0.0 50-51 0.001219369864493169 0.0 0.0 0.23089639362652792 0.0 52-53 0.001219369864493169 0.0 0.0 0.293737490571658 0.0 54-55 0.001219369864493169 0.0 0.0 0.3961210102653523 0.0 56-57 0.001219369864493169 0.0 0.0 0.5511987276746443 0.0 58-59 0.001219369864493169 0.0 0.0 0.7504786026718135 0.0 60-61 0.001219369864493169 0.0 0.0 0.98890896010396 0.0 62-63 0.001219369864493169 0.0 0.0 1.2386184887826683 0.0 64-65 0.001219369864493169 0.0 0.0 1.5071411525135567 0.0 66-67 0.001219369864493169 0.0 0.0 1.8449501539018964 0.0 68-69 0.001219369864493169 0.0 0.0 2.269508611364179 0.0 70-71 0.001219369864493169 0.0 0.0 2.588199635234215 0.0 72-73 0.0013064677119569669 0.0 0.0 2.9531831650312594 0.0 74-75 0.0013935655594207645 0.0 0.0 3.359973661610927 0.0 76-77 0.0013935655594207645 0.0 0.0 3.8072646572612605 0.0 78-79 0.0013935655594207645 0.0 0.0 4.3247565179674154 0.0 80-81 0.0014806634068845622 0.0 0.0 5.00899720764301 0.0 82-83 0.0014806634068845622 0.0 0.0 5.677081246614071 0.0 84-85 0.0014806634068845622 0.0 0.0 6.554243668421979 0.0 86-87 0.0014806634068845622 0.0 0.0 7.56383836729859 0.0 88-89 0.0014806634068845622 0.0 0.0 8.868172181992694 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3805 0.0 46.959297 1 GTATCAA 6675 0.0 39.86806 1 CGCTATA 75 3.0703584E-4 31.680872 1 ATCAACG 8740 0.0 30.053423 3 TATCAAC 8855 0.0 29.770403 2 TCAACGC 8855 0.0 29.663122 4 CAACGCA 9085 0.0 28.912155 5 AACGCAG 9330 0.0 28.202621 6 GTAAGGT 285 0.0 24.999231 3 ACGCAGA 10745 0.0 24.359213 7 CGCAGAG 10805 0.0 24.092058 8 GTACATG 6495 0.0 23.559448 1 GCAGAGT 11175 0.0 22.529236 9 AGTACTT 6170 0.0 22.442513 12-13 TAAGGTG 360 0.0 22.429867 5 GAGTACT 6105 0.0 22.136791 12-13 TACATGG 6890 0.0 21.991343 2 ACATGGG 6760 0.0 21.641346 3 GTACTTT 6400 0.0 21.039452 14-15 AGAGTAC 10235 0.0 20.722956 10-11 >>END_MODULE