FastQCFastQC Report
Thu 26 May 2016
SRR937482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080341
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61020.5648216627898044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42540.3937645613746031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29920.2769495927674688No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16600.15365518850066787No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30350.044.0243951
GTATCAA56450.042.0322231
TCAACGC71500.032.7721634
ATCAACG71500.032.7675973
TATCAAC72400.032.3602642
CAACGCA72400.032.236475
AACGCAG74350.031.562276
ACGCAGA83750.027.9076127
GTACATG62000.027.5326821
CGCAGAG85000.027.329548
TACATGG64900.025.9216292
GCAGAGT88400.025.5809829
CTACACT2850.025.0155834
ACATGGG64600.024.7914033
GTGTAGC3550.022.7701741
AGTACTT47750.022.5308112-13
GAGTACT45700.022.39819512-13
CATGGGA40750.022.277684
AGAGTAC80600.021.9839710-11
GTAAGAC2858.367351E-1121.677153
Produced by FastQC (version 0.11.5)