##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937482_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1080341 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.762917449212797 33.0 31.0 34.0 30.0 34.0 2 31.85558633801735 33.0 31.0 34.0 30.0 34.0 3 31.88965613634954 33.0 31.0 34.0 30.0 34.0 4 35.51942025712252 37.0 35.0 37.0 33.0 37.0 5 35.52178617677196 37.0 35.0 37.0 33.0 37.0 6 35.56209474601075 37.0 35.0 37.0 33.0 37.0 7 35.504010307856504 37.0 35.0 37.0 33.0 37.0 8 35.508215461599626 37.0 35.0 37.0 33.0 37.0 9 37.12651838632432 39.0 37.0 39.0 34.0 39.0 10-11 37.01088221219041 39.0 37.0 39.0 33.0 39.0 12-13 36.873217808080966 39.0 37.0 39.0 33.0 39.0 14-15 38.047956154584526 40.0 38.0 41.0 33.0 41.0 16-17 38.029802164316635 40.0 38.0 41.0 33.0 41.0 18-19 38.02358190608335 40.0 38.0 41.0 33.0 41.0 20-21 37.973991545262095 40.0 38.0 41.0 33.0 41.0 22-23 37.82515751970905 40.0 38.0 41.0 33.0 41.0 24-25 37.664692907146915 40.0 37.0 41.0 32.0 41.0 26-27 37.514770799219875 40.0 37.0 41.0 32.0 41.0 28-29 37.54388197800509 40.0 37.0 41.0 32.0 41.0 30-31 37.35914817636284 39.5 37.0 41.0 32.0 41.0 32-33 37.15367740370864 39.0 36.0 41.0 31.5 41.0 34-35 37.025792319276974 39.0 36.0 41.0 31.0 41.0 36-37 37.03645608192228 40.0 36.0 41.0 31.0 41.0 38-39 36.9524178014164 40.0 36.0 41.0 31.0 41.0 40-41 36.75669117436068 39.5 36.0 41.0 30.5 41.0 42-43 36.48988930346992 39.0 35.0 41.0 30.0 41.0 44-45 36.22221224594827 39.0 35.0 41.0 30.0 41.0 46-47 35.81907703215929 39.0 35.0 41.0 28.5 41.0 48-49 35.450442499173874 39.0 35.0 41.0 26.5 41.0 50-51 34.535386975038435 38.0 34.0 40.0 25.0 40.5 52-53 34.587831064450945 38.0 34.5 40.0 25.5 40.5 54-55 34.89431114805418 39.0 35.0 40.0 25.5 41.0 56-57 34.701691410397274 38.5 34.5 40.0 24.5 41.0 58-59 34.4734023794339 38.0 34.0 40.0 23.5 41.0 60-61 34.18870708415213 38.0 34.0 40.0 23.0 41.0 62-63 33.86142106982888 37.5 34.0 40.0 22.0 41.0 64-65 33.48933716298835 37.0 33.0 40.0 21.0 41.0 66-67 33.114939171983664 36.5 33.0 39.5 19.5 41.0 68-69 32.65658667031983 36.0 32.5 39.0 18.0 41.0 70-71 32.22688160497472 35.5 32.0 39.0 15.0 40.0 72-73 31.83799235611719 35.0 31.5 38.0 12.0 40.0 74-75 31.387861795488647 35.0 31.0 37.0 7.5 39.5 76-77 30.972654467432044 35.0 31.0 37.0 7.0 39.0 78-79 30.518956051839186 35.0 31.0 36.0 2.0 39.0 80-81 30.045872090386275 34.0 30.0 36.0 2.0 37.5 82-83 29.587768584178512 34.0 29.5 35.0 2.0 37.0 84-85 29.168635180928987 34.0 29.0 35.0 2.0 36.5 86-87 28.76204318821557 34.0 29.0 35.0 2.0 36.0 88-89 28.390133763321025 34.0 29.0 35.0 2.0 36.0 90-91 28.010660985744316 34.0 28.0 35.0 2.0 35.5 92-93 27.601256455137776 34.0 27.0 35.0 2.0 35.0 94-95 27.103633019574374 33.0 26.0 35.0 2.0 35.0 96-97 26.518620509635383 33.0 25.0 35.0 2.0 35.0 98-99 25.78656646373691 33.0 23.5 35.0 2.0 35.0 100-101 23.9753786073101 30.5 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.028394818370820474 1101 2 0.04907019632541676 1101 3 -0.02848238782770096 1101 4 -0.09364685541361695 1101 5 0.006912609182826657 1101 6 -0.10533238330916816 1101 7 -0.09283754388930276 1101 8 0.024353644350092907 1101 9 0.16208382365918794 1101 10-11 0.05910378618474965 1101 12-13 -0.059307734769419085 1101 14-15 0.055282132645366744 1101 16-17 0.1825259088172544 1101 18-19 0.18420245755732623 1101 20-21 0.05461458921703155 1101 22-23 0.007366364090330535 1101 24-25 0.07855780882728425 1101 26-27 -0.02581024298550716 1101 28-29 -0.021258343088192078 1101 30-31 -0.009408829095356452 1101 32-33 0.0472404244729816 1101 34-35 -0.010241467952717187 1101 36-37 0.04981371675095403 1101 38-39 0.17620158786846218 1101 40-41 0.15716494720533802 1101 42-43 0.20810820286298082 1101 44-45 0.32134415557094087 1101 46-47 0.4624141219641871 1101 48-49 0.5480676990909785 1101 50-51 0.45494944355954914 1101 52-53 0.46440129015331166 1101 54-55 0.5430431077164357 1101 56-57 0.5135406706677017 1101 58-59 0.6548699592926539 1101 60-61 0.5940272270438456 1101 62-63 0.5626735994868284 1101 64-65 0.5665411695488061 1101 66-67 0.5707476719265259 1101 68-69 0.7879698627718099 1101 70-71 0.7916605070330149 1101 72-73 0.7316822771555138 1101 74-75 0.6810268834434936 1101 76-77 0.676479070402138 1101 78-79 0.6598251366191583 1101 80-81 0.6157043072986532 1101 82-83 0.5530099514323581 1101 84-85 0.7270600425266522 1101 86-87 0.7804995248810407 1101 88-89 0.8175702567189624 1101 90-91 0.9038991207243399 1101 92-93 1.1379384096865799 1101 94-95 1.0975049021393346 1101 96-97 1.0920049759376589 1101 98-99 1.197324841207795 1101 100-101 1.5457353724735903 1102 1 -0.04686243716180272 1102 2 -0.023941147797248874 1102 3 0.051040951194426754 1102 4 -0.15975344895375798 1102 5 0.011716381046369406 1102 6 -0.1774310548086646 1102 7 -0.016004493234383688 1102 8 -0.03779298561241262 1102 9 0.07991246593977763 1102 10-11 0.03893305194296204 1102 12-13 0.05446654167069909 1102 14-15 0.08392274006052247 1102 16-17 0.14814287868074416 1102 18-19 0.0042513142508155966 1102 20-21 0.07662458247149573 1102 22-23 0.1374489836826811 1102 24-25 0.24512619851137174 1102 26-27 0.215191689090652 1102 28-29 0.16439356970243324 1102 30-31 0.21608964479343484 1102 32-33 0.25070618515972143 1102 34-35 0.2189456510698662 1102 36-37 0.329872559653964 1102 38-39 0.43565449505307186 1102 40-41 0.5535268495518366 1102 42-43 0.5559608155688878 1102 44-45 0.5093304108832726 1102 46-47 0.803302894611484 1102 48-49 0.6476878884943318 1102 50-51 0.5879879459451516 1102 52-53 0.7342230354327626 1102 54-55 0.769069336046627 1102 56-57 0.7811473091193619 1102 58-59 0.7940155483645341 1102 60-61 0.8490889319557553 1102 62-63 0.7063355266667557 1102 64-65 0.649239929462361 1102 66-67 0.764346546848806 1102 68-69 0.7832331059474598 1102 70-71 0.7571782312339863 1102 72-73 0.7238921531116418 1102 74-75 0.8538743445333807 1102 76-77 0.915064410223863 1102 78-79 0.8375738539988653 1102 80-81 0.7117643337524235 1102 82-83 0.7986615472004601 1102 84-85 0.9203765032167404 1102 86-87 0.7966047094321809 1102 88-89 0.8355891125142207 1102 90-91 0.8080671743645524 1102 92-93 0.7581702588261301 1102 94-95 0.6643998250236081 1102 96-97 0.7180165860069998 1102 98-99 0.8235136316435749 1102 100-101 1.1062904613015405 1103 1 -0.0012092525964852996 1103 2 -0.014112613873219715 1103 3 0.1018700229439986 1103 4 -0.015421127461699768 1103 5 -0.11578473562531144 1103 6 0.06907353493983237 1103 7 -0.02461881762523177 1103 8 0.04755354723596383 1103 9 0.10172630537606153 1103 10-11 0.002768386169641701 1103 12-13 -0.0064645346184804 1103 14-15 0.08897921174264667 1103 16-17 -0.0256002959484789 1103 18-19 -0.06299602168960661 1103 20-21 -0.1039963781396196 1103 22-23 -0.22436996053843217 1103 24-25 -0.13224410804918563 1103 26-27 -0.09839797736635347 1103 28-29 -0.09608263379616488 1103 30-31 -0.07881103979467241 1103 32-33 0.07562300451414927 1103 34-35 0.010564688270982003 1103 36-37 0.2412562298119454 1103 38-39 0.10609140993989996 1103 40-41 0.33609856722077325 1103 42-43 0.35994072459433113 1103 44-45 0.4212610222724109 1103 46-47 0.4042983276521994 1103 48-49 0.5117884892181195 1103 50-51 0.5433712150128898 1103 52-53 0.5829126553997597 1103 54-55 0.6260195917416524 1103 56-57 0.6130585952516654 1103 58-59 0.5659364971252572 1103 60-61 0.6274829301873197 1103 62-63 0.3909227227725367 1103 64-65 0.5844602100432184 1103 66-67 0.5496192426309747 1103 68-69 0.697786300540038 1103 70-71 0.9053254678134195 1103 72-73 0.9275384279860717 1103 74-75 0.9497786662806753 1103 76-77 0.9339940462344742 1103 78-79 0.9307370886093054 1103 80-81 0.8365535334862422 1103 82-83 0.769330912110533 1103 84-85 0.7890413285145961 1103 86-87 0.9280027823759092 1103 88-89 0.8966541894050408 1103 90-91 0.9754722116932903 1103 92-93 1.0740862486530212 1103 94-95 1.122621719081092 1103 96-97 1.1835137100076913 1103 98-99 1.2000489412578936 1103 100-101 1.5938903920223169 1104 1 -0.018856348881744367 1104 2 -0.019721134068774404 1104 3 -0.07224672003845001 1104 4 -0.11496280819212501 1104 5 -0.015591345697295367 1104 6 -0.014939635105321258 1104 7 0.054547928647608046 1104 8 -0.00821110719894591 1104 9 0.05699188218115836 1104 10-11 0.0804587032993993 1104 12-13 0.1804730477739085 1104 14-15 0.13305874149306618 1104 16-17 0.07831866284524835 1104 18-19 0.24076308791004664 1104 20-21 0.18010743680044072 1104 22-23 0.17956555633913496 1104 24-25 0.21810796351569905 1104 26-27 0.12104121307071836 1104 28-29 0.10921107052315904 1104 30-31 0.24076210129506848 1104 32-33 0.08132068362272804 1104 34-35 0.048721518798998886 1104 36-37 0.3278401485159961 1104 38-39 0.373628649707328 1104 40-41 0.32322571844490255 1104 42-43 0.3635771345470147 1104 44-45 0.5190418144705831 1104 46-47 0.5470459930685223 1104 48-49 0.561135824746458 1104 50-51 0.4664461785532552 1104 52-53 0.5335992851716256 1104 54-55 0.5672935876955378 1104 56-57 0.6500552984947134 1104 58-59 0.5370543163831627 1104 60-61 0.4939303083845701 1104 62-63 0.5232334882867136 1104 64-65 0.5984953089353056 1104 66-67 0.5451804024610709 1104 68-69 0.6756526003814614 1104 70-71 0.8048041004553426 1104 72-73 0.9315771144629963 1104 74-75 0.844565841832452 1104 76-77 0.7067258541012649 1104 78-79 0.787605190734471 1104 80-81 0.8101019945397177 1104 82-83 0.6797102832854591 1104 84-85 0.7637913120626543 1104 86-87 0.8570099978793699 1104 88-89 0.5167990089537717 1104 90-91 0.7970694723918292 1104 92-93 0.8783272409492682 1104 94-95 0.7853685778215294 1104 96-97 1.0185060359726812 1104 98-99 1.0836413661468178 1104 100-101 1.1296855727707857 1105 1 0.03748727667281315 1105 2 0.12511793113331748 1105 3 0.080117985600058 1105 4 0.05455830298206621 1105 5 0.15936920574443292 1105 6 0.09152416098596206 1105 7 0.22554695268441805 1105 8 0.2633373579795375 1105 9 0.23414767492370459 1105 10-11 0.1854864907647169 1105 12-13 0.04237020125966495 1105 14-15 0.26919073713502684 1105 16-17 0.11644566061846717 1105 18-19 0.1382297289835961 1105 20-21 0.17032933099810066 1105 22-23 0.2696024410790514 1105 24-25 0.20842853543192774 1105 26-27 0.35316364913972365 1105 28-29 0.2625972482529946 1105 30-31 0.1784942079607177 1105 32-33 0.05130328209994417 1105 34-35 0.21379041747454863 1105 36-37 0.15214199239252224 1105 38-39 0.40810096722788813 1105 40-41 0.4370128431203639 1105 42-43 0.450781202772518 1105 44-45 0.39640208186467163 1105 46-47 0.5951881034019237 1105 48-49 0.6702905036473581 1105 50-51 0.6281613865469566 1105 52-53 0.635130096500724 1105 54-55 0.7687930384346302 1105 56-57 0.6868868695971955 1105 58-59 0.7984831337014171 1105 60-61 0.9108580880097392 1105 62-63 0.7897367734887482 1105 64-65 0.7898196900818277 1105 66-67 0.827482486047856 1105 68-69 0.89751660896777 1105 70-71 0.8244427687837614 1105 72-73 0.9094890759147063 1105 74-75 0.760804229177193 1105 76-77 0.8401552517706676 1105 78-79 0.8391109045799006 1105 80-81 1.057335639045803 1105 82-83 1.1574510231250308 1105 84-85 1.1955221012230624 1105 86-87 1.0771667467594455 1105 88-89 1.1061374897494645 1105 90-91 1.168966418609319 1105 92-93 1.375018608586398 1105 94-95 1.1711633119459073 1105 96-97 1.1741843303891422 1105 98-99 1.4303858575420278 1105 100-101 2.036983488463065 1106 1 -0.14694013723950405 1106 2 -0.09691066194323383 1106 3 -0.21404581389234068 1106 4 -0.049177097200931996 1106 5 -0.06589380832852498 1106 6 -0.115333457416952 1106 7 -0.16962211744024813 1106 8 -0.1741855690638161 1106 9 -0.1027985050064899 1106 10-11 -0.49022885368952984 1106 12-13 -0.5808009141849908 1106 14-15 -0.5140684844012853 1106 16-17 -0.508967440194283 1106 18-19 -0.34846285611040884 1106 20-21 -0.33565964745979215 1106 22-23 -0.41931701720097436 1106 24-25 -0.4468044198898369 1106 26-27 -0.3435259549211054 1106 28-29 -0.13874566578974168 1106 30-31 -0.15377796036828073 1106 32-33 -5.194954777252292E-4 1106 34-35 -0.12522074897888302 1106 36-37 -0.09910652226628969 1106 38-39 -0.19832481539682334 1106 40-41 0.005999540259821856 1106 42-43 -0.018494903004480534 1106 44-45 0.0973562194973141 1106 46-47 0.1562852064173228 1106 48-49 0.27695187069949156 1106 50-51 0.2334082119536518 1106 52-53 0.12771146421530943 1106 54-55 0.3443218612991572 1106 56-57 0.13098276777834883 1106 58-59 0.33198270103757466 1106 60-61 0.2526463766896754 1106 62-63 0.2097771830495887 1106 64-65 0.5360789074778083 1106 66-67 0.5997785440455132 1106 68-69 0.2987556008817194 1106 70-71 0.5316560410059168 1106 72-73 0.7304204831693824 1106 74-75 0.5063074234370504 1106 76-77 0.7725419000542963 1106 78-79 0.8286452009525611 1106 80-81 0.7787425624953563 1106 82-83 0.6052028272711496 1106 84-85 0.5071568252911796 1106 86-87 0.5304514726907108 1106 88-89 0.652960412238464 1106 90-91 0.6009713290334133 1106 92-93 0.6183466837084381 1106 94-95 0.573216498546163 1106 96-97 0.6218162721596272 1106 98-99 1.266281430293727 1106 100-101 1.3740776849711196 1107 1 -0.17993737256232123 1107 2 -0.03435306450191433 1107 3 -0.0462028041888658 1107 4 -0.059677449561753804 1107 5 -0.1250470581794474 1107 6 -0.028294914564455098 1107 7 -0.05105541282702575 1107 8 0.06037289572720539 1107 9 0.03484989310702957 1107 10-11 0.1349405505496435 1107 12-13 0.18255085032956941 1107 14-15 0.22833678474817276 1107 16-17 0.1574173378184014 1107 18-19 0.07318022948616232 1107 20-21 -0.11577294501495317 1107 22-23 0.021722176163805784 1107 24-25 0.17558675482691655 1107 26-27 0.13272221551310537 1107 28-29 0.08211409460489705 1107 30-31 0.20093283271459939 1107 32-33 0.30548148029070177 1107 34-35 0.09591276866625975 1107 36-37 0.24768428382683538 1107 38-39 0.29458189030058435 1107 40-41 0.4118107832803659 1107 42-43 0.249708598919959 1107 44-45 0.2457163864621279 1107 46-47 0.1980589365631431 1107 48-49 0.08758503598381395 1107 50-51 0.0037882195429546073 1107 52-53 0.10299741857102873 1107 54-55 0.20563264354016297 1107 56-57 0.30187234331908996 1107 58-59 0.37360464498510026 1107 60-61 0.616434526524337 1107 62-63 0.5070423210123991 1107 64-65 0.4677913810507519 1107 66-67 0.7996457311220055 1107 68-69 0.7355637539689397 1107 70-71 0.7091998150613179 1107 72-73 0.6754982735960056 1107 74-75 0.806833254195439 1107 76-77 0.7072060730905605 1107 78-79 0.9415714220183169 1107 80-81 1.015022174356364 1107 82-83 0.9507413972283274 1107 84-85 0.9600380525910133 1107 86-87 0.833603746773214 1107 88-89 0.6474120845971107 1107 90-91 0.5911269098866647 1107 92-93 0.6211357551021628 1107 94-95 0.7094066649688884 1107 96-97 0.8167043298383838 1107 98-99 1.1310426380781955 1107 100-101 1.2623982791056676 1108 1 -0.09794213877153979 1108 2 -0.08047709550966786 1108 3 -0.06393091112726168 1108 4 -0.03982920128059675 1108 5 -0.020990301940919665 1108 6 -0.11526179762797284 1108 7 -0.06609225507646244 1108 8 8.856669441570375E-5 1108 9 -0.007096856831338982 1108 10-11 0.1587339326407502 1108 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-1.4077960133986736 2316 60-61 -1.5358007925794155 2316 62-63 -1.642469200703644 2316 64-65 -1.6857766342407423 2316 66-67 -1.706874392059479 2316 68-69 -1.6835559232106263 2316 70-71 -1.7948407498744388 2316 72-73 -1.8651250671706876 2316 74-75 -2.0226081815059516 2316 76-77 -2.20063081763897 2316 78-79 -1.7448149005897307 2316 80-81 -1.8089238806505392 2316 82-83 -2.047230355983949 2316 84-85 -2.154372056785647 2316 86-87 -2.541011311100842 2316 88-89 -2.4816789210310084 2316 90-91 -2.3223115524686477 2316 92-93 -2.090571795073057 2316 94-95 -2.1316786063489417 2316 96-97 -2.035679656569176 2316 98-99 -2.403979131062723 2316 100-101 -3.056514325757881 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4213.0 3 782.0 4 611.0 5 745.0 6 868.0 7 982.0 8 1274.0 9 1549.0 10 2047.0 11 2605.0 12 3644.0 13 5058.0 14 5624.0 15 7398.0 16 9682.0 17 13376.0 18 11000.0 19 6850.0 20 6821.0 21 6938.0 22 7446.0 23 8307.0 24 9271.0 25 10539.0 26 11902.0 27 14084.0 28 16587.0 29 19762.0 30 24670.0 31 30664.0 32 38940.0 33 51028.0 34 69414.0 35 98547.0 36 146037.0 37 214499.0 38 190283.0 39 26291.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45721602514623 19.42033266362185 14.851246954484697 22.271204356747226 2 15.170466625753315 20.770458455462165 40.45445387638536 23.604621042399167 3 18.370728935729325 26.07479863192255 30.2265646178927 25.327907814455425 4 12.343591729035001 16.88526918888205 37.96915517947318 32.80198390260976 5 13.148385419568084 37.93474829628805 34.08955861326203 14.827307670881845 6 30.503615278343492 36.61576916403705 18.120927418868476 14.75968813875098 7 27.687038352997494 33.65836869418721 21.340545896130934 17.314047056684363 8 26.467174643347857 33.88021386603777 20.545594046040762 19.10701744457361 9 27.009648986907397 16.517013517487573 20.845095770903125 35.62824172470191 10-11 24.37184390884847 27.172448542143385 27.560690670125954 20.89501687888219 12-13 25.531394724719465 24.782925968210257 28.295542635209394 21.390136671860887 14-15 22.575487194093228 25.73228133148217 27.020920203194876 24.671311271229722 16-17 21.270484369788594 29.32826899856151 27.385353168232186 22.01589346341771 18-19 21.34039258833887 27.27782967800112 30.540817850625697 20.840959883034316 20-21 22.16618715013189 26.5127730125033 30.38412498011355 20.93691485725126 22-23 21.88323443698657 26.443799308057987 30.352543482929423 21.320422772026024 24-25 21.47670865282093 26.667955979699347 30.556811630902143 21.29852373657758 26-27 21.26265847001505 27.040555801620002 30.314099338408703 21.382686389956245 28-29 21.049975369023972 27.002507628180805 30.708518606823752 21.238998395971468 30-31 21.27531358858347 26.71511696683678 30.572723928389678 21.436845516190072 32-33 21.08776133969951 27.02374607274348 30.239399712492123 21.649092875064884 34-35 21.513147242904004 27.009571120462695 30.004031459300123 21.473250177333178 36-37 21.677542936917632 26.759706317512883 29.976670516263386 21.5860802293061 38-39 21.3050102617024 27.14400009276115 30.084667741150007 21.466321904386444 40-41 21.3061213131709 27.016425071481407 30.166801551836485 21.510652063511206 42-43 21.432460913656612 27.05515223590396 29.644906576821107 21.867480273618316 44-45 21.26436515034009 27.33038268723399 29.512985687727518 21.89226647469841 46-47 21.587526011442716 27.417985677587065 29.04955139248774 21.94493691848248 48-49 21.63494301246911 27.638013088413828 28.729564720012135 21.99747917910493 50-51 21.68776813681101 27.736665528554248 28.478402461291807 22.097163873342936 52-53 21.831147331048037 27.89073824207926 28.297887740879386 21.980226685993316 54-55 21.510140480881322 27.74691081693044 28.39733595216701 22.345612750021225 56-57 21.57575588931633 28.125088406412104 28.200312118113285 22.09884358615828 58-59 21.753422929476663 27.39530858540558 28.532636673694483 22.318631811423277 60-61 21.747518425148357 27.49938320041998 28.892608236732315 21.860490137699344 62-63 21.63896774780031 27.530528486374244 28.92336676250324 21.907137003322198 64-65 21.434510942784758 27.35322857483394 28.93272196640388 22.279538515977425 66-67 21.44638901268192 27.660191144683882 28.53309045681715 22.360329385817042 68-69 21.21856299643476 27.450639406114703 28.91819422957777 22.412603367872762 70-71 21.350287016342563 27.570079804317793 28.773614439447364 22.30601873989228 72-73 21.421402665825674 27.47757608362938 28.59070207496591 22.51031917557903 74-75 21.14438371222047 27.741031819098882 28.410297319923288 22.70428714875736 76-77 21.219977931753554 27.61522402887888 28.541320449212655 22.62347759015491 78-79 21.204079209537202 27.45057752316215 28.472049585734272 22.87329368156638 80-81 21.233819271190615 27.58176227958982 28.427155934707987 22.757262514511577 82-83 21.446894083130854 27.317895307048403 28.41802975710408 22.817180852716664 84-85 21.11575328140224 27.137867820113815 28.3565650536864 23.389813844797548 86-87 21.372332423465863 26.94937482551599 28.504496934182544 23.17379581683561 88-89 21.264693238432184 26.56341807379047 28.615590518974432 23.55629816880292 90-91 21.008519499311298 26.65133125872472 29.063179716449543 23.276969525514442 92-93 21.13297287470778 26.694606152035384 28.91370371933385 23.25871725392299 94-95 20.83656213465852 26.767725249759668 28.692299523319946 23.70341309226187 96-97 21.00154051765474 27.001768673791442 28.501941645821915 23.4947491627319 98-99 21.21859320953815 27.29008571791654 28.043514759231996 23.447806313313315 100-101 21.20257405604883 27.048456229500367 28.090821868068726 23.65814784638207 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1681.0 1 977.0 2 294.0 3 337.0 4 305.5 5 284.5 6 431.5 7 839.0 8 1367.5 9 1647.5 10 1583.0 11 1516.5 12 1616.5 13 1699.5 14 1800.5 15 1946.5 16 2043.0 17 2155.5 18 2291.5 19 2418.5 20 2669.0 21 3012.5 22 3426.0 23 3957.5 24 4577.5 25 5420.5 26 6691.5 27 8309.5 28 9637.0 29 11232.0 30 13507.5 31 15438.0 32 17522.5 33 20370.5 34 23644.5 35 26184.0 36 29203.5 37 32220.5 38 33566.5 39 33764.0 40 33528.5 41 34723.5 42 36471.5 43 38307.0 44 39867.0 45 41038.5 46 40671.5 47 39829.5 48 41145.5 49 40350.0 50 37745.0 51 35780.0 52 33736.5 53 32967.0 54 32349.5 55 29750.5 56 26193.0 57 23035.0 58 20617.0 59 18292.5 60 15618.0 61 13011.0 62 10567.5 63 8166.5 64 6156.0 65 4796.0 66 3677.0 67 2915.0 68 2194.5 69 1462.5 70 1105.0 71 854.0 72 663.0 73 545.5 74 439.5 75 339.0 76 205.0 77 132.0 78 90.5 79 59.5 80 40.0 81 25.0 82 21.0 83 16.5 84 10.5 85 8.0 86 6.5 87 6.5 88 6.0 89 7.5 90 7.0 91 5.0 92 5.0 93 3.5 94 3.0 95 5.5 96 5.0 97 4.0 98 5.5 99 3.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.347853131557536 2 0.302497081939869 3 0.2970358433124356 4 0.30286733540613564 5 0.30370040570523565 6 0.23640683821126848 7 0.22280002332596838 8 0.22863151541966842 9 0.22252233322626835 10-11 0.24649624516703525 12-13 0.23515723276261846 14-15 0.2276596000707184 16-17 0.22923317730235174 18-19 0.2238182203582017 20-21 0.21710737628211832 22-23 0.21849582678061832 24-25 0.20803616635858493 26-27 0.20905436339081826 28-29 0.2249289807570017 30-31 0.23469441592978516 32-33 0.22747447333758508 34-35 0.2376564436599185 36-37 0.21345112330273497 38-39 0.21321971488631827 40-41 0.20951718022365162 42-43 0.21687596786570165 44-45 0.2207636292615017 46-47 0.22678024808833508 48-49 0.2330282753315851 50-51 0.2344630075133685 52-53 0.2524203006273019 54-55 0.23210264166591846 56-57 0.20581464556098492 58-59 0.20771219457560158 60-61 0.2031765896138349 62-63 0.21201639112095164 64-65 0.20461132179561822 66-67 0.19924264653475152 68-69 0.19928892821803487 70-71 0.20979487032335162 72-73 0.20836013814156826 74-75 0.21229408122065163 76-77 0.21488585548451833 78-79 0.2118775460711016 80-81 0.21497841885108498 82-83 0.20785103962545157 84-85 0.20192698416518487 86-87 0.20012199851713489 88-89 0.20053853366668487 90-91 0.20604605397740158 92-93 0.20155673069891822 94-95 0.19942777326788486 96-97 0.19734509752013485 98-99 0.21011884210633494 100-101 0.19628061880461817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1080341.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.51791527288854 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5015072353448 38.89680252725874 2 13.068582802334511 13.465322830947951 3 4.628429086227081 7.15341052532459 4 2.0891546061163644 4.305155599594708 5 1.149134796431545 2.9600514539844176 6 0.730012567941638 2.256523537401666 7 0.4828798150644376 1.7413872979634446 8 0.3365193358641852 1.386941970619185 9 0.24163642453428197 1.1203744361400778 >10 1.5737292201974722 15.169930960504418 >50 0.13875995879756936 4.880869935207275 >100 0.05785733970767435 4.849914775795411 >500 8.984057191569397E-4 0.3244507679386421 >1k 7.187245753255518E-4 0.9240135707741869 >5k 1.7968114383138794E-4 0.5648498105453905 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6102 0.5648216627898044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4254 0.3937645613746031 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2992 0.2769495927674688 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1660 0.15365518850066787 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.256336656666738E-5 0.0 0.0 0.0 0.0 8 9.256336656666738E-5 0.0 0.0 0.0 0.0 9 9.256336656666738E-5 0.0 0.0 0.0 0.0 10-11 1.8512673313333475E-4 0.0 0.0 0.0 0.0 12-13 1.8512673313333475E-4 0.0 0.0 0.0 0.0 14-15 1.8512673313333475E-4 0.0 0.0 0.0 0.0 16-17 1.8512673313333475E-4 0.0 0.0 0.0 0.0 18-19 2.7769009970000214E-4 0.0 0.0 0.0 0.0 20-21 3.702534662666695E-4 0.0 0.0 0.0 0.0 22-23 3.702534662666695E-4 0.0 0.0 0.0 0.0 24-25 4.6281683283333686E-4 0.0 0.0 0.0 0.0 26-27 4.6281683283333686E-4 0.0 0.0 7.40506932533339E-4 0.0 28-29 4.6281683283333686E-4 0.0 0.0 0.002082675747750016 0.0 30-31 4.6281683283333686E-4 0.0 0.0 0.005414956944150041 0.0 32-33 4.6281683283333686E-4 0.0 0.0 0.009580308439650072 0.0 34-35 5.090985161166706E-4 0.0 0.0 0.014763856967383446 0.0 36-37 5.553801994000043E-4 0.0 0.0 0.022909433225250176 0.0 38-39 6.479435659666716E-4 0.0 0.0 0.032721150081316916 0.0 40-41 6.479435659666716E-4 0.0 0.0 0.04762385209855037 0.0 42-43 6.942252492500053E-4 0.0 0.0 0.07206058087215056 0.0 44-45 7.40506932533339E-4 0.0 0.0 0.08997159230280069 0.0 46-47 7.867886158166727E-4 0.0 0.0 0.13250445924018436 0.0 48-49 9.256336656666737E-4 0.0 0.0 0.18744081729750145 0.0 50-51 9.256336656666737E-4 0.0 0.0 0.21881979856360168 0.0 52-53 9.256336656666737E-4 0.0 0.0 0.2762090858349354 0.0 54-55 9.256336656666737E-4 0.0 0.0 0.3662732415043028 0.0 56-57 9.256336656666737E-4 0.0 0.0 0.5017860101579039 0.0 58-59 9.256336656666737E-4 0.0 0.0 0.687097870024372 0.0 60-61 9.256336656666737E-4 0.0 0.0 0.9114251888986903 0.0 62-63 9.256336656666737E-4 0.0 0.0 1.1394087607523922 0.0 64-65 9.256336656666737E-4 0.0 0.0 1.4000672010041275 0.0 66-67 0.001203323765366676 0.0 0.0 1.730148166180863 0.0 68-69 0.001203323765366676 0.0 0.0 2.134835204810333 0.0 70-71 0.0012496054486500095 0.0 0.0 2.426502372861902 0.0 72-73 0.0013884504985000106 0.0 0.0 2.7730133356042215 0.0 74-75 0.001481013865066678 0.0 0.0 3.1783020361163743 0.0 76-77 0.001481013865066678 0.0 0.0 3.6111746198653947 0.0 78-79 0.001481013865066678 0.0 0.0 4.124207079061149 0.0 80-81 0.0016661405982000127 0.0 0.0 4.815146328798036 0.0 82-83 0.0016661405982000127 0.0 0.0 5.479149638864026 0.0 84-85 0.0017124222814833465 0.0 0.0 6.376551477727865 0.0 86-87 0.0018512673313333475 0.0 0.0 7.427978758558639 0.0 88-89 0.0019438306979000148 0.0 0.0 8.81781770755715 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3035 0.0 44.024395 1 GTATCAA 5645 0.0 42.032223 1 TCAACGC 7150 0.0 32.772163 4 ATCAACG 7150 0.0 32.767597 3 TATCAAC 7240 0.0 32.360264 2 CAACGCA 7240 0.0 32.23647 5 AACGCAG 7435 0.0 31.56227 6 ACGCAGA 8375 0.0 27.907612 7 GTACATG 6200 0.0 27.532682 1 CGCAGAG 8500 0.0 27.32954 8 TACATGG 6490 0.0 25.921629 2 GCAGAGT 8840 0.0 25.580982 9 CTACACT 285 0.0 25.015583 4 ACATGGG 6460 0.0 24.791403 3 GTGTAGC 355 0.0 22.770174 1 AGTACTT 4775 0.0 22.53081 12-13 GAGTACT 4570 0.0 22.398195 12-13 CATGGGA 4075 0.0 22.27768 4 AGAGTAC 8060 0.0 21.98397 10-11 GTAAGAC 285 8.367351E-11 21.67715 3 >>END_MODULE