FastQCFastQC Report
Thu 26 May 2016
SRR937482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080341
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74560.6901524611210719No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57070.5282591329959707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39780.36821707220220284No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.12551592506440098No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA200.002074166771.2489242
GGTATCA35250.050.1663781
GTATCAA64250.041.1368061
ATCAACG84100.031.4026183
TATCAAC84700.031.1240882
TCAACGC85200.030.9971854
CAACGCA87650.030.239135
AACGCAG89550.029.543136
CGTGCAA1056.899643E-527.1462179
ACGCAGA101850.025.8868567
CGCAGAG102950.025.702558
GTCTATA1551.4010529E-624.5350671
GTACATG61000.024.2359181
GCAGAGT109000.023.8401079
TACATGG63150.023.4675792
CATATAG1652.429977E-623.0299533
TGACCGT1452.3344486E-522.9306875
ACCGTGC1252.2538917E-422.8028227
GAGTACT61350.022.76565412-13
ACATGGG63150.022.564983
Produced by FastQC (version 0.11.5)