##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937482_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1080341 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11636603627929 33.0 31.0 34.0 31.0 34.0 2 32.33960295869545 34.0 31.0 34.0 31.0 34.0 3 32.40562563116646 34.0 31.0 34.0 31.0 34.0 4 36.0581584888475 37.0 35.0 37.0 35.0 37.0 5 35.89809791538042 37.0 35.0 37.0 35.0 37.0 6 35.982681394115374 37.0 35.0 37.0 35.0 37.0 7 35.93914051211608 37.0 35.0 37.0 35.0 37.0 8 35.92590395069705 37.0 35.0 37.0 35.0 37.0 9 37.60482569855259 39.0 37.0 39.0 35.0 39.0 10-11 37.52812815583228 39.0 37.0 39.0 35.0 39.0 12-13 37.43382552360782 39.0 37.0 39.0 34.0 39.0 14-15 38.61657152695307 40.0 38.0 41.0 34.0 41.0 16-17 38.59243655475447 40.0 38.0 41.0 34.0 41.0 18-19 38.630835541740986 40.0 38.0 41.0 34.0 41.0 20-21 38.55216871339697 40.0 38.0 41.0 34.0 41.0 22-23 38.41990306764253 40.0 38.0 41.0 34.0 41.0 24-25 38.26336684435748 40.0 38.0 41.0 34.0 41.0 26-27 38.02803836936671 40.0 37.0 41.0 33.0 41.0 28-29 37.984373915273046 40.0 37.0 41.0 33.0 41.0 30-31 38.07143855504882 40.0 37.0 41.0 33.5 41.0 32-33 37.99695512805679 40.0 37.0 41.0 33.0 41.0 34-35 37.861918135107345 40.0 37.0 41.0 33.0 41.0 36-37 37.679806190823086 40.0 37.0 41.0 33.0 41.0 38-39 37.50896383641832 40.0 36.5 41.0 33.0 41.0 40-41 37.3215396805268 40.0 36.0 41.0 33.0 41.0 42-43 37.0652516196275 40.0 36.0 41.0 31.5 41.0 44-45 36.7035130574513 40.0 36.0 41.0 31.0 41.0 46-47 36.25115958757466 39.0 35.0 41.0 30.0 41.0 48-49 35.85067400015366 39.0 35.0 41.0 28.5 41.0 50-51 35.60481875630009 39.0 35.0 41.0 28.0 41.0 52-53 35.35001170926587 39.0 35.0 40.5 27.0 41.0 54-55 35.122715883225766 39.0 35.0 40.0 26.5 41.0 56-57 34.8677278748099 38.0 34.5 40.0 26.0 41.0 58-59 34.60797146456535 38.0 34.0 40.0 26.0 41.0 60-61 34.273173933045214 38.0 34.0 40.0 25.0 41.0 62-63 33.915795568251134 37.0 34.0 40.0 23.5 41.0 64-65 33.56495171431983 37.0 33.0 39.5 23.0 41.0 66-67 33.168873531597896 36.0 33.0 39.0 22.0 41.0 68-69 32.721495805491045 36.0 32.5 39.0 20.5 40.0 70-71 32.335060411481194 35.0 32.0 38.5 19.5 40.0 72-73 31.909305950621146 35.0 32.0 37.5 18.0 40.0 74-75 31.468969519809022 35.0 31.0 37.0 16.0 39.0 76-77 30.123424918613658 33.5 29.5 35.5 13.0 38.5 78-79 30.749727632293876 34.5 31.0 36.0 11.0 38.5 80-81 30.584123901619954 34.5 31.0 36.0 7.5 37.5 82-83 30.253519953422114 34.0 31.0 35.0 6.5 37.0 84-85 29.933667703067826 34.0 30.5 35.0 2.0 36.5 86-87 29.602282057239336 34.0 30.0 35.0 2.0 36.0 88-89 29.269798147066528 34.0 30.0 35.0 2.0 36.0 90-91 28.836618252940507 34.0 29.0 35.0 2.0 35.0 92-93 28.447693830003676 34.0 29.0 35.0 2.0 35.0 94-95 28.04772567180177 34.0 29.0 35.0 2.0 35.0 96-97 27.64799586426878 33.5 28.0 35.0 2.0 35.0 98-99 27.01081186403182 33.0 26.5 35.0 2.0 35.0 100-101 25.500861302125905 32.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06535374044810993 1101 2 0.14874732911277988 1101 3 0.1442335736639393 1101 4 0.12646489089097912 1101 5 -0.04277594207162139 1101 6 -1.342675204583088E-4 1101 7 -0.01776574352118132 1101 8 0.03169080061459084 1101 9 0.0889887725782117 1101 10-11 0.050353378449081276 1101 12-13 0.12268769193479301 1101 14-15 0.15011604199552409 1101 16-17 0.12077328497308315 1101 18-19 0.14488034449940557 1101 20-21 0.04677223960681687 1101 22-23 0.033818084660701686 1101 24-25 0.04620946555942851 1101 26-27 0.0979258193765915 1101 28-29 0.12739892851238466 1101 30-31 0.14578656954672908 1101 32-33 0.27397460054518064 1101 34-35 0.3283162914525448 1101 36-37 0.34450394067621204 1101 38-39 0.39809662464556794 1101 40-41 0.47297136522085736 1101 42-43 0.5170566374411933 1101 44-45 0.6255773254915127 1101 46-47 0.6636894932976745 1101 48-49 0.7475932976709458 1101 50-51 0.6889000305391733 1101 52-53 0.8169784090190717 1101 54-55 0.9174275097006372 1101 56-57 0.8685405625995699 1101 58-59 0.9051975408540756 1101 60-61 0.9312704627719057 1101 62-63 0.9789432011061621 1101 64-65 0.9022817464697965 1101 66-67 0.9428281461067556 1101 68-69 0.8539394196017156 1101 70-71 0.5350574691140721 1101 72-73 0.6941391577716729 1101 74-75 0.7240563459010367 1101 76-77 0.7346871448780767 1101 78-79 0.8053914272271676 1101 80-81 0.7343324902555928 1101 82-83 0.7857188376808573 1101 84-85 0.8028008620281426 1101 86-87 0.6295562016265279 1101 88-89 0.6012575282998043 1101 90-91 0.542365224616411 1101 92-93 0.6352761740522084 1101 94-95 0.3070244154431556 1101 96-97 0.326803293460749 1101 98-99 0.17231739886583242 1101 100-101 0.42272499420788634 1102 1 0.10522372598835972 1102 2 0.07836110761618897 1102 3 -0.03377897690555898 1102 4 -0.003751758779756642 1102 5 -0.12326796102956905 1102 6 -0.003266626185649102 1102 7 0.026990439738966643 1102 8 0.04213246582823871 1102 9 0.03609391662092065 1102 10-11 -0.0014415854719942445 1102 12-13 -0.07149635373053798 1102 14-15 0.024563586215755606 1102 16-17 -0.00969046614309832 1102 18-19 0.10337874969997785 1102 20-21 0.09094880001809713 1102 22-23 0.011184261527567685 1102 24-25 0.08083343281451505 1102 26-27 0.042808371337464735 1102 28-29 -0.022836020402955626 1102 30-31 0.02855850882635025 1102 32-33 0.021203031125175187 1102 34-35 0.22427768867648012 1102 36-37 0.1967682740570993 1102 38-39 0.1105726057862455 1102 40-41 0.16050419026140617 1102 42-43 0.42008517122962985 1102 44-45 0.27588386866029424 1102 46-47 0.5302064548833485 1102 48-49 0.5325991009443811 1102 50-51 0.49628533443168266 1102 52-53 0.6408036204114538 1102 54-55 0.6324129883077845 1102 56-57 0.7437678941681511 1102 58-59 0.6007716422273148 1102 60-61 0.5622689252126563 1102 62-63 0.4962788733612342 1102 64-65 0.6005078293218773 1102 66-67 0.7404745180481385 1102 68-69 0.6791589136689922 1102 70-71 0.6181764599961497 1102 72-73 0.6727141591366319 1102 74-75 0.6706351444913459 1102 76-77 0.569373636019936 1102 78-79 0.7215691747116857 1102 80-81 0.7813364512759158 1102 82-83 0.7721121428696343 1102 84-85 0.7034389579204152 1102 86-87 0.8330625588087628 1102 88-89 0.9694231831362465 1102 90-91 0.8421584554810195 1102 92-93 0.7688944754102991 1102 94-95 0.5414959477010797 1102 96-97 0.7140804750600545 1102 98-99 0.6528415603852871 1102 100-101 0.7611350029612289 1103 1 0.040529694028755614 1103 2 0.02051477987077277 1103 3 0.11541747526899115 1103 4 0.027338203937084415 1103 5 -0.09050618128280519 1103 6 -0.032017503570543226 1103 7 -0.00717150955957635 1103 8 -0.03168819214206309 1103 9 -0.04871296754604515 1103 10-11 0.01913798991929383 1103 12-13 -0.07663942444092697 1103 14-15 -0.026264573965100624 1103 16-17 -0.11214896697718046 1103 18-19 -0.09861302795017224 1103 20-21 -0.09495105504247192 1103 22-23 -0.09656589571803664 1103 24-25 -0.1250744440507816 1103 26-27 -0.0538179128349654 1103 28-29 -0.1535212466087259 1103 30-31 -0.030390817068585818 1103 32-33 0.09843386767177975 1103 34-35 0.20375098427086868 1103 36-37 0.3224608383910592 1103 38-39 0.30266292401488926 1103 40-41 0.3653801713372431 1103 42-43 0.3720454997589968 1103 44-45 0.4326554568864722 1103 46-47 0.5809425466236249 1103 48-49 0.5183521934412596 1103 50-51 0.5736064162814358 1103 52-53 0.578111393357915 1103 54-55 0.6117869229118398 1103 56-57 0.5423622552040541 1103 58-59 0.6205271594969801 1103 60-61 0.5875020589124418 1103 62-63 0.6564250889836956 1103 64-65 0.6774779572149612 1103 66-67 0.8308100664934628 1103 68-69 0.7108614505647353 1103 70-71 0.7250934434329537 1103 72-73 0.7277729097405441 1103 74-75 0.7550856052893309 1103 76-77 0.3435908263828331 1103 78-79 0.738881687815482 1103 80-81 0.7186150540233811 1103 82-83 0.6858576875775242 1103 84-85 0.6520447135653029 1103 86-87 0.8391162866690358 1103 88-89 0.6129597051369586 1103 90-91 0.8650661773796315 1103 92-93 0.9113599041666411 1103 94-95 0.8039693982621507 1103 96-97 0.8373901987635755 1103 98-99 0.8792624681254182 1103 100-101 1.077495838322804 1104 1 -0.029912215755160787 1104 2 0.028025356877535046 1104 3 -0.09779545107512888 1104 4 -0.04093204946528317 1104 5 -0.16890021697609114 1104 6 -0.049764372925139355 1104 7 -0.09824309355258265 1104 8 -0.146108797191431 1104 9 -0.08906798984638442 1104 10-11 -0.009762234888533783 1104 12-13 -0.09211592576026106 1104 14-15 0.04365511932824262 1104 16-17 -0.05693731008616254 1104 18-19 -0.005431349682233133 1104 20-21 -0.2238451236396486 1104 22-23 -0.159976988360512 1104 24-25 -0.16595380567295592 1104 26-27 -0.3223093652548883 1104 28-29 -0.2969906746543032 1104 30-31 -0.138260364841841 1104 32-33 -0.0023114583844829895 1104 34-35 -0.004080564822793065 1104 36-37 -0.03632630319541619 1104 38-39 -0.16274341773122813 1104 40-41 -0.16677966087416962 1104 42-43 0.02460064560811759 1104 44-45 0.20928584436686748 1104 46-47 0.2723677943049694 1104 48-49 0.2801503588904808 1104 50-51 0.3973328767362716 1104 52-53 0.4892740892687044 1104 54-55 0.39080974122507683 1104 56-57 0.48843915688937045 1104 58-59 0.4808384939299444 1104 60-61 0.36579356185574596 1104 62-63 0.4025790494438368 1104 64-65 0.555424712361237 1104 66-67 0.756748729406965 1104 68-69 0.8083737046082007 1104 70-71 0.7293263689703977 1104 72-73 0.5839118193270387 1104 74-75 0.6318701103193796 1104 76-77 0.6201182565469914 1104 78-79 0.7194323077883524 1104 80-81 0.6934085556123186 1104 82-83 0.756028936375472 1104 84-85 0.7175255666887317 1104 86-87 0.7272398948877878 1104 88-89 0.7379700539393639 1104 90-91 0.7722029238010713 1104 92-93 0.7220893302167575 1104 94-95 0.6499717885701521 1104 96-97 0.665013116000754 1104 98-99 0.7931614935335851 1104 100-101 1.0767018994306277 1105 1 0.07582163556860877 1105 2 0.05990411755748681 1105 3 -0.006048603129833907 1105 4 0.07527245402358318 1105 5 -0.08499261536732661 1105 6 0.01804389131126527 1105 7 0.07061295683343616 1105 8 0.023664640569066364 1105 9 0.02303440428233472 1105 10-11 -0.010151538764539225 1105 12-13 0.013900891806713389 1105 14-15 0.11455843949553213 1105 16-17 -0.036684046286822536 1105 18-19 0.010956811514802212 1105 20-21 0.0010058009120044176 1105 22-23 -0.06480570817256393 1105 24-25 0.009953421050589384 1105 26-27 -0.07807570362664507 1105 28-29 -0.051921181208939515 1105 30-31 -0.10112374891985354 1105 32-33 0.007930297520182705 1105 34-35 0.11586918613988217 1105 36-37 0.11214806718216863 1105 38-39 0.13011853349224367 1105 40-41 0.07739317202344864 1105 42-43 -0.04356853576333464 1105 44-45 0.03680710513467744 1105 46-47 0.08180174466691881 1105 48-49 0.22966701830276293 1105 50-51 0.3509597560061124 1105 52-53 0.30976580169209456 1105 54-55 0.3398203957151864 1105 56-57 0.3860716125767638 1105 58-59 0.38880958090781803 1105 60-61 0.4326168512391959 1105 62-63 0.42762201255099086 1105 64-65 0.5334646394388542 1105 66-67 0.5225198169255449 1105 68-69 0.5457566994183836 1105 70-71 0.39897184110498074 1105 72-73 0.4664177467489026 1105 74-75 0.4502494887488808 1105 76-77 0.3732383198449192 1105 78-79 0.3501748778968441 1105 80-81 0.5119145479181633 1105 82-83 0.41169506504580866 1105 84-85 0.32324725226637696 1105 86-87 0.43395259313312806 1105 88-89 0.42827341357774174 1105 90-91 0.29256612989872366 1105 92-93 0.4916534270272557 1105 94-95 0.5992870372965804 1105 96-97 0.5167536738047218 1105 98-99 0.489608104382004 1105 100-101 0.3578689639622503 1106 1 -0.27014382584978236 1106 2 -0.09521477184204485 1106 3 -0.15557308693495742 1106 4 -0.10078239698686531 1106 5 -0.1949120032618481 1106 6 -0.19915004559847915 1106 7 -0.13313113802966825 1106 8 -0.0696139576828898 1106 9 -0.1862349527667746 1106 10-11 -0.3188723029027116 1106 12-13 -0.37502332784574577 1106 14-15 -0.19075856440051098 1106 16-17 -0.1382201064374442 1106 18-19 -0.23802777405948206 1106 20-21 -0.3276557979982613 1106 22-23 -0.19176377083223173 1106 24-25 -0.27694038378282926 1106 26-27 -0.23971063471095277 1106 28-29 -0.10269417967287353 1106 30-31 -0.17862874836090725 1106 32-33 -0.1982882618955344 1106 34-35 -0.12255260521263978 1106 36-37 -0.17848196589901733 1106 38-39 -0.18484004256757913 1106 40-41 -0.3507486092266845 1106 42-43 -0.503872352727889 1106 44-45 -0.21575575533226754 1106 46-47 -0.17349724882034678 1106 48-49 -0.2935591634491814 1106 50-51 0.019419389918674312 1106 52-53 0.0036965009513849623 1106 54-55 0.1282819341767194 1106 56-57 0.1974860714157316 1106 58-59 0.038696136034161555 1106 60-61 0.12243620890935603 1106 62-63 0.10418219008807483 1106 64-65 0.018020817423092694 1106 66-67 0.04555016135908119 1106 68-69 0.11835479404134475 1106 70-71 0.040786420841410376 1106 72-73 0.1028770245936137 1106 74-75 -0.12480340388838229 1106 76-77 -0.05145912598929314 1106 78-79 0.03475838201853776 1106 80-81 0.08122294822123521 1106 82-83 -0.23362471286721842 1106 84-85 -0.15716177032737022 1106 86-87 0.10722804473895309 1106 88-89 -0.17099685434261502 1106 90-91 0.036425408571503226 1106 92-93 0.0632238932691287 1106 94-95 -0.11401085486454932 1106 96-97 -0.04475828966106121 1106 98-99 -0.18673139389354887 1106 100-101 0.06338449638719368 1107 1 -0.13787299590453372 1107 2 -0.02865110295279294 1107 3 0.022450439925776777 1107 4 -0.030787275828949134 1107 5 -0.12395195555761518 1107 6 -0.19686241075270772 1107 7 -0.09482138576642285 1107 8 -0.0364053058695859 1107 9 -0.08770402632887198 1107 10-11 -0.13791876032126282 1107 12-13 -0.022998608379168672 1107 14-15 -0.04020562896385371 1107 16-17 -0.11497242388744411 1107 18-19 0.0344067681425031 1107 20-21 -0.1024240529990692 1107 22-23 -0.049372054021908696 1107 24-25 -0.15276843170389753 1107 26-27 -0.22391402821666873 1107 28-29 -0.20152325757172207 1107 30-31 -0.3750590622330847 1107 32-33 -0.09607923061673063 1107 34-35 0.0068966277249629115 1107 36-37 0.016102519383139224 1107 38-39 0.004353322207180099 1107 40-41 7.990679023919256E-4 1107 42-43 0.04446070955865622 1107 44-45 0.04567157702529556 1107 46-47 0.099370968233913 1107 48-49 0.04119716549319463 1107 50-51 0.12318151926220366 1107 52-53 0.031823025589538645 1107 54-55 0.1301304732345656 1107 56-57 0.2677685021632854 1107 58-59 0.3791878148979322 1107 60-61 0.36986126447394696 1107 62-63 0.25787384726810103 1107 64-65 0.5450333397844602 1107 66-67 0.5842881675389506 1107 68-69 0.3843167390730571 1107 70-71 0.2972183609942789 1107 72-73 0.5881293691482838 1107 74-75 0.6890497971741247 1107 76-77 0.6975922102713419 1107 78-79 0.8110255257275405 1107 80-81 0.6178820255779875 1107 82-83 0.5796918155655888 1107 84-85 0.4854169687629941 1107 86-87 0.2748216914023658 1107 88-89 0.5307242927273705 1107 90-91 0.5998774603956463 1107 92-93 0.5102938775484134 1107 94-95 0.4923750073352693 1107 96-97 0.4140019423549859 1107 98-99 0.3827270546346675 1107 100-101 0.36047887487692165 1108 1 -0.02766810369207917 1108 2 -0.01665340388138503 1108 3 -0.09055665460403617 1108 4 -0.07178489272433808 1108 5 -0.08285709703733346 1108 6 -0.052157285708197776 1108 7 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-0.3288207025511092 2316 96-97 -0.5057124040398335 2316 98-99 -0.18603688292506604 2316 100-101 -0.3643432553131589 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 11.0 5 28.0 6 53.0 7 137.0 8 361.0 9 667.0 10 1155.0 11 1847.0 12 2878.0 13 4015.0 14 5316.0 15 7440.0 16 10389.0 17 14652.0 18 12709.0 19 6944.0 20 5835.0 21 5933.0 22 5828.0 23 6196.0 24 7459.0 25 8570.0 26 10211.0 27 12441.0 28 15660.0 29 19084.0 30 23567.0 31 29604.0 32 37983.0 33 49564.0 34 67774.0 35 97375.0 36 148419.0 37 232441.0 38 206802.0 39 20987.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.91919932734014 20.209891359506322 15.266341448959711 21.60456786419383 2 15.75613625697812 21.503951067301898 39.47651713671887 23.26339553900111 3 18.821834957666145 26.35760375659167 30.45973447272667 24.36082681301552 4 12.925641070735999 17.694135462784434 37.25805093021555 32.12217253626402 5 13.27973916351992 38.41857700813818 33.62444768041976 14.677236147922128 6 30.69854795846867 37.076719295111445 17.579912268441166 14.644820477978712 7 27.77187943436378 34.21577076126889 20.916821633169526 17.0955281711978 8 27.038407317689504 34.22900732268793 19.842531200796785 18.890054158825777 9 27.238159062740376 16.553939913416226 20.73447180103319 35.4734292228102 10-11 24.72788684313564 27.465448409344827 27.035167599859673 20.771497147659858 12-13 25.868437971096732 25.05196672833401 28.139329614161795 20.940265686407468 14-15 22.825107998307942 25.81421051316205 27.16878281949866 24.191898669031353 16-17 21.320814446549747 29.47472140740748 27.53334363872148 21.671120507321298 18-19 21.34265940105948 27.114309278274174 31.09282161835939 20.450209702306957 20-21 22.223677524041022 26.251479856822986 31.09097035102806 20.43387226810794 22-23 21.885517678029924 26.271701822786497 31.091552163980435 20.75122833520314 24-25 21.67496963907525 26.465146028642938 31.19149968158055 20.668384650701263 26-27 21.47935350427398 26.785252284862814 31.062795023495433 20.672599187367776 28-29 21.071729411002398 26.94652460867714 31.284399848191725 20.69734613212874 30-31 21.39780049299614 26.487990277071976 31.235045120347156 20.879164109584732 32-33 21.169704750629663 26.734197813468153 31.093099308459088 21.002998127443096 34-35 21.6319662032636 26.629647490931102 30.823138249867405 20.915248055937894 36-37 21.898156825991876 26.504263713885706 30.648015087877628 20.949564372244783 38-39 21.595807604478974 26.808949429668704 30.767529021036953 20.82771394481537 40-41 21.39607836603367 26.89804742116027 30.7424835040434 20.963390708762656 42-43 21.542358932391785 26.842675815513477 30.35190412225782 21.26306112983691 44-45 21.53010021835698 27.100008238139623 30.029175973141815 21.340715570361578 46-47 21.737118187683333 27.38801915321181 29.446535862287927 21.428326796816933 48-49 22.076280580057862 27.513362685190458 28.954112152603738 21.456244582147942 50-51 21.911286302790646 27.722787398972827 28.69835945241338 21.667566845823146 52-53 22.161135457881528 27.959312790099776 28.43036841659497 21.449183335423726 54-55 21.83231961019715 27.895636655463413 28.48470992029368 21.787333814045752 56-57 21.948995733754433 28.1450486466773 28.336839942203433 21.56911567736483 58-59 21.996573304169704 27.515432627290824 28.62522111074189 21.862772957797585 60-61 22.09663430342827 27.578838533388993 28.940584500634518 21.383942662548215 62-63 21.849721523111683 27.58915934876118 29.03509169789909 21.526027430228048 64-65 21.754705227331 27.375754507141725 29.029075079072257 21.84046518645502 66-67 21.7252700767628 27.690053418318843 28.639938686025985 21.944737818892367 68-69 21.61155598093565 27.484840434640546 28.958310385332037 21.94529319909177 70-71 21.666862592459232 27.71592487927423 28.655952148442022 21.96126037982452 72-73 21.735590892134983 27.545099186275447 28.459902938053816 22.259406983535754 74-75 21.52630512032775 27.824131454790663 28.2253010854906 22.42426233939099 76-77 21.52519435992895 27.682000405427548 28.570469879417704 22.222335355225802 78-79 21.508810644046648 27.556391916996574 28.263159502416368 22.671637936540407 80-81 21.423143248289197 27.781043207653877 28.204242919596684 22.59157062446024 82-83 21.599804135463096 27.623517999219686 28.20949174726255 22.567186118054668 84-85 21.171833708060696 27.394267180455063 28.17267881159745 23.261220299886794 86-87 21.25976890629903 27.06386224349534 28.290373132186968 23.385995718018663 88-89 21.241904176551664 26.82546529290289 28.42445116865878 23.508179361886665 90-91 21.139309055227447 26.953881472978725 28.72192805435728 23.184881417436543 92-93 20.82090643680842 26.84700245668405 28.626987609203958 23.705103497303572 94-95 20.51384701682154 27.01651608149649 28.495262144082282 23.974374757599684 96-97 20.785844469477695 27.288420970786078 28.185128584400665 23.740605975335566 98-99 20.681116901016683 27.592348068203325 27.727075607095436 23.999459423684556 100-101 20.342447492371953 27.436716829102114 27.6960611873093 24.524774491216636 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 406.0 1 299.0 2 240.5 3 296.0 4 276.0 5 288.0 6 480.0 7 921.5 8 1501.0 9 1822.0 10 1719.5 11 1638.5 12 1746.0 13 1904.5 14 2098.5 15 2252.0 16 2334.5 17 2464.5 18 2618.5 19 2802.0 20 3034.5 21 3318.5 22 3758.0 23 4315.5 24 4895.5 25 5733.0 26 7047.0 27 8530.5 28 9816.5 29 11597.0 30 13718.5 31 15310.0 32 17311.0 33 20098.0 34 23387.5 35 25926.0 36 29255.5 37 32268.5 38 33205.0 39 33282.0 40 32933.0 41 34157.0 42 36222.5 43 38178.5 44 39926.5 45 41504.0 46 40929.0 47 39720.0 48 40857.5 49 40169.5 50 37888.0 51 35738.0 52 33582.0 53 32678.0 54 31549.0 55 29273.0 56 25958.5 57 22769.5 58 20698.0 59 18548.0 60 15842.5 61 13026.0 62 10667.0 63 8349.5 64 6109.0 65 4716.0 66 3632.5 67 2953.0 68 2209.5 69 1371.5 70 987.5 71 776.5 72 699.5 73 580.0 74 449.5 75 361.5 76 238.0 77 134.0 78 91.0 79 69.5 80 35.5 81 22.5 82 12.5 83 4.5 84 3.0 85 1.0 86 2.0 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04156095158843365 2 0.0 3 0.0 4 0.0 5 0.011477857454266754 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007636477741750059 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015735772316333451 24-25 4.6281683283333686E-4 26-27 0.0022678024808833507 28-29 0.0028694643635666884 30-31 7.40506932533339E-4 32-33 0.0 34-35 0.0 36-37 3.239717829833358E-4 38-39 3.702534662666695E-4 40-41 4.628168328333369E-5 42-43 0.012218364386800093 44-45 0.0 46-47 0.0 48-49 4.628168328333369E-5 50-51 4.628168328333369E-5 52-53 1.3884504985000107E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.3140841641666843E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.007497632691900057 92-93 0.00212895743103335 94-95 0.0 96-97 0.0 98-99 0.0011570420820833421 100-101 0.010829913888300083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1080341.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.54930353312367 #Duplication Level Percentage of deduplicated Percentage of total 1 74.40492462401606 36.8671219455456 2 13.541474352818753 13.419412459876517 3 4.747085727789765 7.056443748720431 4 2.22571833693883 4.411311738248852 5 1.2054340597581281 2.986420905806052 6 0.7681018961936539 2.2835348399320323 7 0.5384379695549424 1.8675458471065651 8 0.37501894934978725 1.4865542201604593 9 0.27912080402832296 1.2447217297088013 >10 1.7034360875939507 15.707079010702532 >50 0.14215414376710547 4.836876286009663 >100 0.0666588761016385 5.543955952855272 >500 0.0014979520548078103 0.47391054145150024 >1k 5.617320205529288E-4 0.5938726264352101 >5k 3.744880137019526E-4 1.2212381474405047 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7456 0.6901524611210719 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5707 0.5282591329959707 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3978 0.36821707220220284 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1356 0.12551592506440098 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.256336656666738E-5 0.0 0.0 0.0 0.0 8 9.256336656666738E-5 0.0 0.0 0.0 0.0 9 9.256336656666738E-5 0.0 0.0 0.0 0.0 10-11 9.256336656666738E-5 0.0 0.0 0.0 0.0 12-13 9.256336656666738E-5 0.0 0.0 0.0 0.0 14-15 9.256336656666738E-5 0.0 0.0 0.0 0.0 16-17 9.256336656666738E-5 0.0 0.0 0.0 0.0 18-19 1.3884504985000107E-4 0.0 0.0 0.0 0.0 20-21 1.8512673313333475E-4 0.0 0.0 0.0 0.0 22-23 1.8512673313333475E-4 0.0 0.0 0.0 0.0 24-25 1.8512673313333475E-4 0.0 0.0 9.256336656666738E-5 0.0 26-27 1.8512673313333475E-4 0.0 0.0 8.330702991000065E-4 0.0 28-29 2.7769009970000214E-4 0.0 0.0 0.0022215207976000167 0.0 30-31 2.7769009970000214E-4 0.0 0.0 0.005877773776983378 0.0 32-33 2.7769009970000214E-4 0.0 0.0 0.010089406955766744 0.0 34-35 2.7769009970000214E-4 0.0 0.0 0.015458082216633452 0.0 36-37 2.7769009970000214E-4 0.0 0.0 0.023696221841066848 0.0 38-39 3.702534662666695E-4 0.0 0.0 0.03360050206370026 0.0 40-41 3.702534662666695E-4 0.0 0.0 0.04859576744750037 0.0 42-43 3.702534662666695E-4 0.0 0.0 0.07326390463751722 0.0 44-45 3.702534662666695E-4 0.0 0.0 0.09117491606816737 0.0 46-47 4.6281683283333686E-4 0.0 0.0 0.13343009290585103 0.0 48-49 4.6281683283333686E-4 0.0 0.0 0.18832016927988476 0.0 50-51 4.6281683283333686E-4 0.0 0.0 0.21951402381285168 0.0 52-53 4.6281683283333686E-4 0.0 0.0 0.2777363813832855 0.0 54-55 4.6281683283333686E-4 0.0 0.0 0.36905014250130286 0.0 56-57 4.6281683283333686E-4 0.0 0.0 0.5058125166035539 0.0 58-59 4.6281683283333686E-4 0.0 0.0 0.6916334749861386 0.0 60-61 4.6281683283333686E-4 0.0 0.0 0.9173029626756737 0.0 62-63 4.6281683283333686E-4 0.0 0.0 1.1463047315616088 0.0 64-65 4.6281683283333686E-4 0.0 0.0 1.409045847561094 0.0 66-67 6.479435659666716E-4 0.0 0.0 1.7394507845208134 0.0 68-69 6.479435659666716E-4 0.0 0.0 2.146359343947883 0.0 70-71 6.479435659666716E-4 0.0 0.0 2.438443047149002 0.0 72-73 6.479435659666716E-4 0.0 0.0 2.788147446037871 0.0 74-75 6.479435659666716E-4 0.0 0.0 3.196120484180458 0.0 76-77 6.479435659666716E-4 0.0 0.0 3.632186504076028 0.0 78-79 6.479435659666716E-4 0.0 0.0 4.149060342984298 0.0 80-81 8.330702991000064E-4 0.0 0.0 4.848330295712187 0.0 82-83 9.256336656666737E-4 0.0 0.0 5.522052759267676 0.0 84-85 9.256336656666737E-4 0.0 0.0 6.431117582318916 0.0 86-87 9.256336656666737E-4 0.0 0.0 7.4954574527857405 0.0 88-89 9.256336656666737E-4 0.0 0.0 8.905104962229519 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTATA 20 0.0020741667 71.248924 2 GGTATCA 3525 0.0 50.166378 1 GTATCAA 6425 0.0 41.136806 1 ATCAACG 8410 0.0 31.402618 3 TATCAAC 8470 0.0 31.124088 2 TCAACGC 8520 0.0 30.997185 4 CAACGCA 8765 0.0 30.23913 5 AACGCAG 8955 0.0 29.54313 6 CGTGCAA 105 6.899643E-5 27.146217 9 ACGCAGA 10185 0.0 25.886856 7 CGCAGAG 10295 0.0 25.70255 8 GTCTATA 155 1.4010529E-6 24.535067 1 GTACATG 6100 0.0 24.235918 1 GCAGAGT 10900 0.0 23.840107 9 TACATGG 6315 0.0 23.467579 2 CATATAG 165 2.429977E-6 23.029953 3 TGACCGT 145 2.3344486E-5 22.930687 5 ACCGTGC 125 2.2538917E-4 22.802822 7 GAGTACT 6135 0.0 22.765654 12-13 ACATGGG 6315 0.0 22.56498 3 >>END_MODULE