FastQCFastQC Report
Thu 26 May 2016
SRR937481_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937481_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247135
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70510.5653758414285542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48840.39161758751057424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.264847029391365No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18750.1503445897998212No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38050.043.984531
GTATCAA69500.042.2097051
ATCAACG86900.033.3616373
TCAACGC87650.033.133064
TATCAAC88150.032.9451262
CAACGCA88900.032.665875
AACGCAG90500.032.1752746
GTACATG73900.028.0513671
ACGCAGA104500.027.728347
CGCAGAG105300.027.2921268
TACATGG75500.026.9444312
ACATGGG76350.025.5840953
GCAGAGT110250.025.548719
CATGGGG29400.023.9269084
GAGTACT57900.022.92773812-13
AGTACTT58700.022.61526312-13
ATGGGGA13700.022.550055
GTGTAGC3950.021.6664311
GTACTTT60850.021.66009714-15
GTATAGA2901.05501385E-1021.3136251
Produced by FastQC (version 0.11.5)