FastQCFastQC Report
Thu 26 May 2016
SRR937481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247135
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87300.7000044101079674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64210.5148600592558142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.3698075990169468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.12452541224486523No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39000.050.5800741
GTATCAA71500.040.88511
TATCAAC93850.030.8766542
ATCAACG94900.030.5850833
TCAACGC96050.030.218894
CAACGCA97150.029.925635
AACGCAG99800.029.125176
TACGTTG700.00838796927.1454245
GACCCGT908.953512E-426.3860936
ACGCAGA114050.025.404877
CGCAGAG115200.024.821418
ACTTGAC5150.023.9799658
GCAGAGT120400.023.5126829
GAGTACT68600.023.16084512-13
GTACATG70550.023.1096361
AGTACTT69400.022.68853612-13
GTAAGGT2354.645699E-922.2361454
TACATGG73500.021.7809722
GTACTTT72400.021.58265914-15
AGAGTAC109750.021.18510410-11
Produced by FastQC (version 0.11.5)