##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937481_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1247135 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07501593652652 31.0 31.0 34.0 30.0 34.0 2 32.294838970921354 33.0 31.0 34.0 31.0 34.0 3 32.36526839516171 34.0 31.0 34.0 30.0 34.0 4 36.01817124850157 37.0 35.0 37.0 35.0 37.0 5 35.88826951372546 37.0 35.0 37.0 35.0 37.0 6 35.96923508681899 37.0 35.0 37.0 35.0 37.0 7 35.910526125880516 37.0 35.0 37.0 35.0 37.0 8 35.89407481948626 37.0 35.0 37.0 35.0 37.0 9 37.56665557457693 39.0 37.0 39.0 35.0 39.0 10-11 37.49263672336996 39.0 37.0 39.0 35.0 39.0 12-13 37.40471801368737 39.0 37.0 39.0 34.0 39.0 14-15 38.572180638022346 40.0 38.0 41.0 34.0 41.0 16-17 38.55024115272204 40.0 38.0 41.0 34.0 41.0 18-19 38.57719252526792 40.0 38.0 41.0 34.0 41.0 20-21 38.48736584251104 40.0 38.0 41.0 34.0 41.0 22-23 38.35637801841821 40.0 38.0 41.0 34.0 41.0 24-25 38.209319760891965 40.0 38.0 41.0 33.5 41.0 26-27 38.118735742321405 40.0 37.5 41.0 33.0 41.0 28-29 38.01169440357299 40.0 37.0 41.0 33.0 41.0 30-31 38.0539255974694 40.0 37.0 41.0 33.0 41.0 32-33 37.953561563102625 40.0 37.0 41.0 33.0 41.0 34-35 37.81505089665514 40.0 37.0 41.0 33.0 41.0 36-37 37.62787388694889 40.0 37.0 41.0 33.0 41.0 38-39 37.47107049357126 40.0 36.0 41.0 33.0 41.0 40-41 37.27432314865672 40.0 36.0 41.0 32.0 41.0 42-43 37.018594618866445 40.0 36.0 41.0 31.5 41.0 44-45 36.6444266258264 39.5 36.0 41.0 31.0 41.0 46-47 36.18955806708977 39.0 35.0 41.0 30.0 41.0 48-49 35.80131100482305 39.0 35.0 41.0 28.5 41.0 50-51 35.54465915879195 39.0 35.0 41.0 27.5 41.0 52-53 35.28958212222413 39.0 35.0 40.0 27.0 41.0 54-55 35.033805081246214 38.5 35.0 40.0 26.0 41.0 56-57 34.78606004963376 38.0 34.0 40.0 26.0 41.0 58-59 34.53278995457589 38.0 34.0 40.0 25.5 41.0 60-61 34.20104880385844 38.0 34.0 40.0 24.5 41.0 62-63 33.83004726833903 37.0 33.5 40.0 23.0 41.0 64-65 33.46848536846452 36.5 33.0 39.5 22.5 41.0 66-67 33.05625814366528 36.0 33.0 39.0 21.5 40.5 68-69 32.6329282715985 35.5 32.0 39.0 20.0 40.0 70-71 32.226212078082966 35.0 32.0 38.5 18.5 40.0 72-73 31.78474463470274 35.0 31.0 37.5 18.0 40.0 74-75 31.333157998131718 35.0 31.0 37.0 14.0 39.0 76-77 29.991421939084383 33.5 29.5 35.5 11.0 38.0 78-79 30.63345347536554 34.0 31.0 36.0 9.5 38.5 80-81 30.487877815954167 34.0 31.0 36.0 7.0 37.5 82-83 30.172068781647535 34.0 30.5 35.0 6.0 37.0 84-85 29.8144078227297 34.0 30.0 35.0 2.0 36.5 86-87 29.442124148548473 34.0 30.0 35.0 2.0 36.0 88-89 29.061338988962703 34.0 29.0 35.0 2.0 36.0 90-91 28.630044862825596 34.0 29.0 35.0 2.0 35.0 92-93 28.266759813492527 34.0 29.0 35.0 2.0 35.0 94-95 27.854294041944136 33.5 28.5 35.0 2.0 35.0 96-97 27.377327234020374 33.0 27.0 35.0 2.0 35.0 98-99 26.87919671887967 33.0 26.5 35.0 2.0 35.0 100-101 25.06681514030157 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10743092254273989 1101 2 0.10740263815443996 1101 3 0.04015754757296719 1101 4 0.06225584813506657 1101 5 0.06551460417070132 1101 6 0.007885130614361913 1101 7 -0.003236158247815979 1101 8 -0.001331399623872187 1101 9 0.1501926853537512 1101 10-11 0.0392096924253309 1101 12-13 0.04195863655327514 1101 14-15 -0.01653051559705432 1101 16-17 -0.035972049085451374 1101 18-19 -0.024379115881551172 1101 20-21 0.027532565592686353 1101 22-23 -0.04548479904961056 1101 24-25 0.044189693040785016 1101 26-27 0.04046873453631861 1101 28-29 -0.016238359092206167 1101 30-31 0.08299246802429394 1101 32-33 1.5652963904244643E-4 1101 34-35 0.005608207976429469 1101 36-37 0.03739951127085561 1101 38-39 0.0030950148188679805 1101 40-41 -0.0901552521344442 1101 42-43 0.11822723061425222 1101 44-45 0.17010306354313087 1101 46-47 0.2815479745247629 1101 48-49 0.24721192845869666 1101 50-51 0.18610281450732913 1101 52-53 0.19075445568474692 1101 54-55 0.18435091968679984 1101 56-57 0.1764632381865141 1101 58-59 0.18416940048911812 1101 60-61 0.2938241263457897 1101 62-63 0.2825052471400298 1101 64-65 0.38522293155060794 1101 66-67 0.4107381675785078 1101 68-69 0.35625612235238435 1101 70-71 0.2942578217256546 1101 72-73 0.2757637800297772 1101 74-75 0.18026309560723774 1101 76-77 0.10130137697830222 1101 78-79 0.20722430598982555 1101 80-81 0.3216137772852079 1101 82-83 0.2745015781403808 1101 84-85 0.21450902436944475 1101 86-87 0.2540632279101054 1101 88-89 0.14468933171778176 1101 90-91 0.1326272016797212 1101 92-93 0.15552294538114353 1101 94-95 0.10858300549267952 1101 96-97 0.09195447017881264 1101 98-99 0.06282086368518591 1101 100-101 0.36287994050928774 1102 1 0.09143060221973798 1102 2 0.1083623656067445 1102 3 0.1122438808305617 1102 4 0.061462179604816924 1102 5 -0.0763661388296839 1102 6 0.0569736999251802 1102 7 0.047774038701170696 1102 8 0.0857911810373082 1102 9 0.08525030003993805 1102 10-11 0.060956582622822 1102 12-13 0.0515248720254462 1102 14-15 -0.06088081860026762 1102 16-17 -0.09514023976951336 1102 18-19 0.04385123608887653 1102 20-21 0.03370483604959418 1102 22-23 0.11274203930025095 1102 24-25 0.02318665369691786 1102 26-27 0.06929951211420615 1102 28-29 0.007121630578005522 1102 30-31 0.06360283854365179 1102 32-33 0.048103948245341144 1102 34-35 0.1799137402526938 1102 36-37 0.17703318218490693 1102 38-39 -0.05445107506773894 1102 40-41 0.07737786963672733 1102 42-43 0.1796400102605773 1102 44-45 0.24967198078930153 1102 46-47 0.30039504139653417 1102 48-49 0.4134234074446326 1102 50-51 0.49525082980834867 1102 52-53 0.41258310274218246 1102 54-55 0.47306344468875494 1102 56-57 0.4854034470420814 1102 58-59 0.5373844874543323 1102 60-61 0.47326274165647675 1102 62-63 0.3337969682802253 1102 64-65 0.35660027080867707 1102 66-67 0.5055281697760705 1102 68-69 0.5484305258128686 1102 70-71 0.573558064947651 1102 72-73 0.7922428777121553 1102 74-75 0.7445555231962366 1102 76-77 0.6348950969092222 1102 78-79 0.5874078274818828 1102 80-81 0.677348924788987 1102 82-83 0.748690589819752 1102 84-85 0.6690908228217154 1102 86-87 0.6372202904736604 1102 88-89 0.6866151744590496 1102 90-91 0.6457405798831068 1102 92-93 0.6844702745949718 1102 94-95 0.6747688151835654 1102 96-97 0.6758123634715218 1102 98-99 0.6389244260991305 1102 100-101 1.0043529855767517 1103 1 0.01865020477257673 1103 2 0.09815940654921462 1103 3 0.12039098386127023 1103 4 0.09992834599026423 1103 5 -0.05784074453850252 1103 6 -0.03263240081038532 1103 7 -0.059913625200060494 1103 8 -0.06641195403999944 1103 9 0.0906326548820644 1103 10-11 -0.004207521443369444 1103 12-13 0.013246414233755388 1103 14-15 0.011363784261980925 1103 16-17 0.0548695238276693 1103 18-19 -0.023567777622794495 1103 20-21 -0.055901780368927234 1103 22-23 -0.039223881620110035 1103 24-25 -0.05897865775495603 1103 26-27 -0.03771174765110885 1103 28-29 -0.10964102187507052 1103 30-31 -5.65825534970088E-4 1103 32-33 0.09229436726867846 1103 34-35 0.10850660650103805 1103 36-37 0.18008722850836278 1103 38-39 0.3211875123185237 1103 40-41 0.2951151972439092 1103 42-43 0.349026216910552 1103 44-45 0.26759780770409947 1103 46-47 0.3579770590507749 1103 48-49 0.5599751730742994 1103 50-51 0.6343202077296866 1103 52-53 0.6172222553169533 1103 54-55 0.6732330012426715 1103 56-57 0.47893076654961675 1103 58-59 0.49899876760906636 1103 60-61 0.46167534577286773 1103 62-63 0.44096723028841467 1103 64-65 0.3621390494789338 1103 66-67 0.5825552163154271 1103 68-69 0.6305167658791007 1103 70-71 0.5530421344699654 1103 72-73 0.5521840417254573 1103 74-75 0.6601210348706097 1103 76-77 0.5543352783746123 1103 78-79 0.6159259540813622 1103 80-81 0.65246859761222 1103 82-83 0.6113238251045701 1103 84-85 0.7404606555066593 1103 86-87 0.6278931329593327 1103 88-89 0.663427146829445 1103 90-91 0.7084386880830174 1103 92-93 0.6975387119106173 1103 94-95 0.7160327334663847 1103 96-97 0.47522579130547626 1103 98-99 0.541411014904547 1103 100-101 0.8228581994751636 1104 1 0.03673737604239591 1104 2 0.054874917485051355 1104 3 0.010060874202785897 1104 4 0.013029866704336257 1104 5 0.038681362464103586 1104 6 -0.05186889364311753 1104 7 0.0040420941493977125 1104 8 0.010584311751529185 1104 9 0.03912202039777668 1104 10-11 -0.004404042391328744 1104 12-13 -0.031839879056214215 1104 14-15 -0.06579340310620552 1104 16-17 -0.05952812446130196 1104 18-19 -0.08173994446131161 1104 20-21 -0.15312997606446999 1104 22-23 -0.04932592763189092 1104 24-25 -0.028348503348887277 1104 26-27 -0.15928929239559153 1104 28-29 -0.08560151154028262 1104 30-31 -0.06877018496774667 1104 32-33 -0.0071504067229142265 1104 34-35 0.03565797792541758 1104 36-37 -0.040316654637940985 1104 38-39 -0.03712233301877177 1104 40-41 0.09228363980867726 1104 42-43 0.1606817408360186 1104 44-45 0.22155602314901301 1104 46-47 0.15828466245556427 1104 48-49 0.14999739370512088 1104 50-51 -0.07270400356831175 1104 52-53 0.09695845155724925 1104 54-55 0.07722967228750832 1104 56-57 0.08050136113775608 1104 58-59 0.13820638355642245 1104 60-61 0.14358947843351189 1104 62-63 0.050041427243179726 1104 64-65 0.016261560161844102 1104 66-67 0.1311580858731176 1104 68-69 0.2429119495325338 1104 70-71 0.11782957517734616 1104 72-73 0.2335436934808186 1104 74-75 0.11040661633873583 1104 76-77 0.19776835341933108 1104 78-79 0.4258912322759585 1104 80-81 0.47608468240164115 1104 82-83 0.48850899523437974 1104 84-85 0.4175064706556846 1104 86-87 0.5324384896629617 1104 88-89 0.5812528282986911 1104 90-91 0.53127479478675 1104 92-93 0.46526005824572536 1104 94-95 0.3892195353720247 1104 96-97 0.4112132578431016 1104 98-99 0.5773910097136614 1104 100-101 0.9941187028419947 1105 1 -0.12338301087421044 1105 2 -0.044256184156516554 1105 3 -0.0475849941183597 1105 4 -0.030175044900680348 1105 5 -0.20240262307512324 1105 6 -0.23030368128470968 1105 7 -0.1264033833672329 1105 8 -0.1159597262619485 1105 9 -0.06799314457274619 1105 10-11 -0.14524670047858024 1105 12-13 -0.0966352933313388 1105 14-15 -0.1464223140217058 1105 16-17 -0.09426298353859153 1105 18-19 -0.14851423126037133 1105 20-21 -0.06598692254872418 1105 22-23 -0.1248261583587933 1105 24-25 -0.13772076572769265 1105 26-27 -0.10861225753993864 1105 28-29 -0.24584661995676527 1105 30-31 -0.15404383148783296 1105 32-33 -0.06782906783368503 1105 34-35 -0.21817732615440377 1105 36-37 -0.21726044629107122 1105 38-39 -0.1579252500863646 1105 40-41 -0.06950546730177365 1105 42-43 -0.2988221475494086 1105 44-45 -0.1551397095512712 1105 46-47 -0.14652194372615668 1105 48-49 0.0876218596281575 1105 50-51 0.23582332962808294 1105 52-53 0.47662280759954 1105 54-55 0.43472254051397385 1105 56-57 0.5480641772076709 1105 58-59 0.4896100744949763 1105 60-61 0.40490885889636985 1105 62-63 0.47034250148377765 1105 64-65 0.34618735951971047 1105 66-67 0.2196117374449713 1105 68-69 0.48074059383026935 1105 70-71 0.5352892640247298 1105 72-73 0.7340705749746306 1105 74-75 0.6040322939319296 1105 76-77 0.5231200350205967 1105 78-79 0.6091079019885797 1105 80-81 0.552652359307217 1105 82-83 0.596405517596331 1105 84-85 0.6209498849987511 1105 86-87 0.6797521961626067 1105 88-89 0.7161624862094769 1105 90-91 0.6242237678082141 1105 92-93 0.45289265544435153 1105 94-95 0.3186328284505642 1105 96-97 0.37043105744128724 1105 98-99 0.5078797701518134 1105 100-101 0.7516461093299647 1106 1 -0.1954693875017135 1106 2 -0.11773756146450864 1106 3 -0.22154586900914097 1106 4 -0.03515740151985369 1106 5 -0.12384569185965688 1106 6 -0.21738278950994072 1106 7 -0.241102124365554 1106 8 -0.16886965132935217 1106 9 -0.3502285579643569 1106 10-11 -0.4359586291397477 1106 12-13 -0.5656089791509586 1106 14-15 -0.4394397660768874 1106 16-17 -0.23580279012216465 1106 18-19 -0.3125678399185503 1106 20-21 -0.3650331050102693 1106 22-23 -0.36566077212754067 1106 24-25 -0.43152349123061384 1106 26-27 -0.2185959061003615 1106 28-29 -0.21872867459700274 1106 30-31 -0.158851874660364 1106 32-33 -0.17904647097260806 1106 34-35 -0.20516841471845026 1106 36-37 -0.15984869771270382 1106 38-39 -0.16616482727640403 1106 40-41 -0.1951248101277585 1106 42-43 -0.5293130950847527 1106 44-45 -0.2632924505377545 1106 46-47 -6.501891121502013E-4 1106 48-49 0.11088962283406545 1106 50-51 0.2067815259597623 1106 52-53 0.3529026677148508 1106 54-55 0.1297911218835921 1106 56-57 0.03189124248917352 1106 58-59 -0.06089373327994707 1106 60-61 -0.0052349016191399755 1106 62-63 -0.014315449674931813 1106 64-65 0.05188413006109016 1106 66-67 -0.039539595226933955 1106 68-69 0.08886621391275895 1106 70-71 0.12527568384843235 1106 72-73 0.13593062519268173 1106 74-75 -0.039627381130056705 1106 76-77 -0.052173265228521615 1106 78-79 0.10215632118504914 1106 80-81 0.2311448907033622 1106 82-83 0.3535819553494193 1106 84-85 0.3625924551531341 1106 86-87 0.4552239026493332 1106 88-89 0.11935614524349702 1106 90-91 0.0238064017509636 1106 92-93 0.10154257684303758 1106 94-95 -0.6207669908749018 1106 96-97 -0.3276003594139105 1106 98-99 -0.017458797351626032 1106 100-101 0.3892099913865792 1107 1 -0.007829140503204712 1107 2 -0.04784103128346828 1107 3 -0.16513410967123576 1107 4 -0.05904765144322255 1107 5 -0.2122454662945117 1107 6 -0.1548475449566098 1107 7 -0.20434483386151925 1107 8 -0.04269766024900434 1107 9 -0.050978608856908636 1107 10-11 -0.059100466091720705 1107 12-13 -0.204612327529766 1107 14-15 -0.3361831693494537 1107 16-17 -0.25396177844846335 1107 18-19 -0.18721744178670718 1107 20-21 -0.03765858731955518 1107 22-23 -0.1238427732460039 1107 24-25 -0.26350963441768016 1107 26-27 -0.22471939131578011 1107 28-29 -0.14261915850910611 1107 30-31 -0.10956594978478762 1107 32-33 -0.26384866859481804 1107 34-35 -0.005483890031470651 1107 36-37 -0.19705846393456028 1107 38-39 0.03757530717351898 1107 40-41 -0.046155004034218905 1107 42-43 0.03318909855963881 1107 44-45 0.1358683488613579 1107 46-47 -0.05829828643274482 1107 48-49 -0.09845073952088512 1107 50-51 -0.22894615560964837 1107 52-53 -0.09202281857223937 1107 54-55 -0.175788515012691 1107 56-57 -0.09879965605097141 1107 58-59 -0.057605899056284215 1107 60-61 -0.029364697510771975 1107 62-63 -0.03708196869470015 1107 64-65 0.11765485385844698 1107 66-67 0.07569780974439055 1107 68-69 0.014089263675138852 1107 70-71 -0.15320220772586524 1107 72-73 -0.07534804135230999 1107 74-75 0.013961011624719077 1107 76-77 -0.06743956780871585 1107 78-79 0.08492217333692764 1107 80-81 0.06845895740418939 1107 82-83 0.039630201486851035 1107 84-85 0.09304942345467992 1107 86-87 0.19791497200125718 1107 88-89 0.11971356303740421 1107 90-91 0.24033489806915043 1107 92-93 0.25316581520034376 1107 94-95 0.16399408322699216 1107 96-97 0.17855880908371802 1107 98-99 0.3174987102939575 1107 100-101 0.7723568941946759 1108 1 -0.11197441570140754 1108 2 -0.007371881964814975 1108 3 -0.16143021402164948 1108 4 -0.12093094283038397 1108 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-0.5657832827989324 2316 70-71 -0.6890194727135004 2316 72-73 -0.7859105587188786 2316 74-75 -0.909616979638475 2316 76-77 -0.943427469289599 2316 78-79 -0.8335335345129877 2316 80-81 -1.0294634316889244 2316 82-83 -1.1028599251922984 2316 84-85 -1.0901684364375441 2316 86-87 -1.0714775055987182 2316 88-89 -0.9535517161259008 2316 90-91 -0.7280385890465659 2316 92-93 -0.6157684127674585 2316 94-95 -0.2320502112006757 2316 96-97 -0.2159731704544825 2316 98-99 -0.1462057323587871 2316 100-101 -0.5122133942839469 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 12.0 5 34.0 6 65.0 7 174.0 8 406.0 9 786.0 10 1380.0 11 2129.0 12 3363.0 13 4841.0 14 6031.0 15 8702.0 16 12214.0 17 17372.0 18 14420.0 19 7958.0 20 6661.0 21 6848.0 22 6972.0 23 7466.0 24 8681.0 25 10228.0 26 12157.0 27 14976.0 28 18210.0 29 22970.0 30 28706.0 31 35645.0 32 45088.0 33 59254.0 34 80740.0 35 114464.0 36 172642.0 37 265876.0 38 227753.0 39 21904.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.865199365930984 20.194825406401016 15.28127186029945 21.65870336736855 2 15.70559722884852 21.530066913365435 39.38402819261748 23.38030766516857 3 18.84054252346378 26.361139732266352 30.448107061384693 24.350210682885173 4 12.934285382095764 17.69014581420616 37.22772594787253 32.14784285582555 5 13.323902185524151 38.415486971222165 33.53984018374699 14.720770659506693 6 30.727066436271933 37.1172326973423 17.499308414886922 14.656392451498835 7 27.80532981593813 34.223159481531674 20.84321264337862 17.128298059151575 8 27.11518801092103 34.199184530944926 19.813251973523315 18.87237548461073 9 27.27820163815465 16.546644910134027 20.641871168718705 35.533282282992616 10-11 24.735694211131914 27.48150761545462 27.031476143320493 20.75132203009297 12-13 25.976916375084247 24.98230859503582 28.183400430367232 20.8573745995127 14-15 22.8704190003488 25.806628793194005 27.111700016437673 24.211252190019525 16-17 21.347929454309277 29.408604521563426 27.504400084994806 21.73906593913249 18-19 21.337144735734302 27.05857826137507 31.071656236093126 20.5326207667975 20-21 22.22369671286589 26.183973667646242 31.11182831048764 20.48050130900023 22-23 21.865551993129777 26.205425854658102 31.158792467293683 20.77022968491843 24-25 21.7261144683736 26.38865780285223 31.227445724404944 20.65778200436923 26-27 21.435916926966502 26.779189965356164 31.033553721195123 20.751339386482208 28-29 21.00001643827488 26.864110394642886 31.35645035901593 20.779422808066304 30-31 21.43192196514411 26.378619796573748 31.280815629422637 20.908642608859505 32-33 21.224967625798328 26.689853143404683 31.082080127652578 21.003099103144407 34-35 21.634626564084883 26.624784004939322 30.816030341542817 20.924559089432982 36-37 21.895424312524305 26.433625870495174 30.691184194173044 20.979765622807474 38-39 21.61077188916998 26.785913313314115 30.805887093217656 20.79742770429825 40-41 21.369178156334318 26.8003463939349 30.818115119854706 21.012360329876074 42-43 21.55288424523944 26.82350478622389 30.36028204075348 21.263328927783196 44-45 21.50392700068557 27.09421995213028 30.056529565764734 21.345323481419413 46-47 21.798522212911994 27.352572095242294 29.394973278754904 21.453932413090804 48-49 22.083415187610004 27.49798538249668 28.94903117946333 21.469568250429987 50-51 21.972080007376906 27.59484739021838 28.669310058654435 21.763762543750275 52-53 22.183043535784016 27.889362418663577 28.417212250478098 21.51038179507431 54-55 21.857617659675977 27.832632393445778 28.436376174191246 21.873373772687 56-57 21.864673832423914 28.128069535375076 28.356954138886326 21.650302493314676 58-59 22.055791874977448 27.51835206292823 28.593095374598583 21.83276068749574 60-61 22.075517085159184 27.54914263491923 28.946505390354694 21.428834889566886 62-63 21.8715696376094 27.55359283477731 29.057439651681655 21.517397875931636 64-65 21.751454333331996 27.425058233471116 28.942095282387232 21.881392150809656 66-67 21.746242387552268 27.714000489121066 28.602156141877185 21.93760098144948 68-69 21.667702373840843 27.547218224169796 28.798405946429217 21.986673455560144 70-71 21.5948554085965 27.842575182317873 28.65102815653478 21.911541252550844 72-73 21.74379678222486 27.609440838401618 28.396244191687348 22.25051818768618 74-75 21.503726541232503 27.903354488487615 28.18941012801341 22.403508842266476 76-77 21.522770189273814 27.76860564413636 28.47081510822806 22.237809058361766 78-79 21.514671627369932 27.588152044485962 28.251632742245224 22.64554358589888 80-81 21.43557032718992 27.89437390499024 28.14282335112077 22.527232416699075 82-83 21.609171439281713 27.679697225435145 28.160159405676623 22.55097192960652 84-85 21.273930039089905 27.464568507567407 28.05560789816578 23.205893555176907 86-87 21.28097599698509 27.12372758362166 28.273362546957625 23.321933872435622 88-89 21.262734186756045 26.93164733569341 28.31766408608531 23.487954391465237 90-91 21.136747376596485 27.017878558600607 28.611186960027553 23.23418710477535 92-93 20.880944868378272 26.883075384757866 28.619041299703195 23.616938447160667 94-95 20.64098914712521 26.989660301410833 28.527304582102182 23.842045969361777 96-97 20.790411623440928 27.159128723033195 28.23752039674935 23.812939256776534 98-99 20.699091996677176 27.41004961784318 27.95947020844626 23.931388177033387 100-101 20.37014983305325 27.385040913682097 27.924154409804046 24.320654843460606 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 448.0 1 333.0 2 280.5 3 353.0 4 317.5 5 341.0 6 556.0 7 1095.5 8 1786.0 9 2115.0 10 1995.5 11 1857.5 12 1948.0 13 2163.5 14 2420.0 15 2583.0 16 2664.0 17 2768.0 18 3001.5 19 3263.0 20 3490.0 21 3850.5 22 4338.0 23 4866.0 24 5531.0 25 6618.0 26 8147.0 27 10007.0 28 11459.0 29 13323.0 30 15731.5 31 17639.0 32 20002.5 33 22967.5 34 26649.5 35 29576.5 36 33287.5 37 36914.5 38 38262.0 39 38484.5 40 38107.0 41 39591.5 42 41867.0 43 44257.5 44 46533.0 45 47980.5 46 47133.0 47 45693.5 48 47172.0 49 46458.5 50 43739.0 51 41463.0 52 39091.5 53 37943.5 54 36706.5 55 33915.5 56 29832.5 57 26334.5 58 23957.5 59 21592.5 60 18402.0 61 14876.5 62 12362.0 63 9778.5 64 7065.0 65 5380.0 66 4076.5 67 3350.0 68 2534.5 69 1572.5 70 1150.5 71 904.0 72 776.5 73 665.0 74 535.5 75 425.5 76 278.0 77 166.5 78 95.5 79 66.5 80 44.0 81 23.5 82 12.0 83 7.0 84 3.5 85 1.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046747144455091066 2 0.0 3 0.0 4 0.0 5 0.016277307588993973 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004851118764207563 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010824810465587126 24-25 3.6082701551957085E-4 26-27 0.0012428486090118552 28-29 0.0036483620458089947 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.017880983213525402 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.004594530664283E-4 84-85 8.018378122657131E-4 86-87 0.0 88-89 0.0 90-91 0.011105453699880126 92-93 0.0015635837339181404 94-95 0.0 96-97 0.0 98-99 2.405513436797139E-4 100-101 0.01447317251139612 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1247135.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.76918109139555 #Duplication Level Percentage of deduplicated Percentage of total 1 73.07517023177003 34.9074104008598 2 13.994512398237887 13.37012794074412 3 5.082058566289248 7.282973279104475 4 2.429797918632188 4.642778271625479 5 1.3762736251667442 3.2871732015950825 6 0.8219219176696728 2.3557522156889825 7 0.532183001810953 1.7795362331088933 8 0.3874650944700562 1.4807112211467843 9 0.3013533287587483 1.2955861560574358 >10 1.765021949784984 15.813518526983447 >50 0.1548013171996848 5.094953474345222 >100 0.07641754206645845 6.207688934958648 >500 0.002183355881364814 0.6776103819221885 >1k 5.03851357238034E-4 0.5886369135878637 >5k 3.35900904825356E-4 1.2155428482717354 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8730 0.7000044101079674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6421 0.5148600592558142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4612 0.3698075990169468 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1553 0.12452541224486523 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.603675624531426E-4 0.0 0.0 0.0 0.0 3 1.603675624531426E-4 0.0 0.0 0.0 0.0 4 1.603675624531426E-4 0.0 0.0 0.0 0.0 5 1.603675624531426E-4 0.0 0.0 0.0 0.0 6 1.603675624531426E-4 0.0 0.0 0.0 0.0 7 1.603675624531426E-4 0.0 0.0 0.0 0.0 8 1.603675624531426E-4 0.0 0.0 0.0 0.0 9 1.603675624531426E-4 0.0 0.0 0.0 0.0 10-11 1.603675624531426E-4 0.0 0.0 0.0 0.0 12-13 2.405513436797139E-4 0.0 0.0 0.0 0.0 14-15 2.405513436797139E-4 0.0 0.0 0.0 0.0 16-17 2.405513436797139E-4 0.0 0.0 8.01837812265713E-5 0.0 18-19 2.806432342929995E-4 0.0 0.0 1.603675624531426E-4 0.0 20-21 3.207351249062852E-4 0.0 0.0 1.603675624531426E-4 0.0 22-23 3.207351249062852E-4 0.0 0.0 1.603675624531426E-4 0.0 24-25 4.009189061328565E-4 0.0 0.0 2.806432342929995E-4 0.0 26-27 4.009189061328565E-4 0.0 0.0 5.211945779727134E-4 0.0 28-29 4.410107967461422E-4 0.0 0.0 0.0022451458743439965 0.0 30-31 4.811026873594278E-4 0.0 0.0 0.006254334935672561 0.0 32-33 4.811026873594278E-4 0.0 0.0 0.011105453699880126 0.0 34-35 5.211945779727135E-4 0.0 0.0 0.016076848135927545 0.0 36-37 6.414702498125704E-4 0.0 0.0 0.02397495058674482 0.0 38-39 7.216540310391417E-4 0.0 0.0 0.03447902592742566 0.0 40-41 7.216540310391417E-4 0.0 0.0 0.05119734431316578 0.0 42-43 8.018378122657129E-4 0.0 0.0 0.07400963007212531 0.0 44-45 8.820215934922843E-4 0.0 0.0 0.0938551159257017 0.0 46-47 0.0010423891559454268 0.0 0.0 0.13819674694399564 0.0 48-49 0.0010423891559454268 0.0 0.0 0.19268162628745084 0.0 50-51 0.0010423891559454268 0.0 0.0 0.22415376041888008 0.0 52-53 0.0010423891559454268 0.0 0.0 0.28469251524494144 0.0 54-55 0.0010423891559454268 0.0 0.0 0.37842735549880324 0.0 56-57 0.0010423891559454268 0.0 0.0 0.5198314536918618 0.0 58-59 0.0010423891559454268 0.0 0.0 0.7041739667317493 0.0 60-61 0.0010423891559454268 0.0 0.0 0.9258019380419923 0.0 62-63 0.0010423891559454268 0.0 0.0 1.1561298496153183 0.0 64-65 0.0010423891559454268 0.0 0.0 1.4113147333688816 0.0 66-67 0.0012027567183985695 0.0 0.0 1.7154919074518797 0.0 68-69 0.0012027567183985695 0.0 0.0 2.1011758951516875 0.0 70-71 0.0012428486090118552 0.0 0.0 2.383743540194125 0.0 72-73 0.001363124280851712 0.0 0.0 2.71594494581581 0.0 74-75 0.0014433080620782834 0.0 0.0 3.0938511067366403 0.0 76-77 0.0014433080620782834 0.0 0.0 3.5004630613365837 0.0 78-79 0.0014433080620782834 0.0 0.0 3.9831694243205424 0.0 80-81 0.0014433080620782834 0.0 0.0 4.629651160459773 0.0 82-83 0.0014433080620782834 0.0 0.0 5.263343583493366 0.0 84-85 0.0014433080620782834 0.0 0.0 6.10775898359039 0.0 86-87 0.0014433080620782834 0.0 0.0 7.110737811062957 0.0 88-89 0.0014433080620782834 0.0 0.0 8.408712769668078 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3900 0.0 50.580074 1 GTATCAA 7150 0.0 40.8851 1 TATCAAC 9385 0.0 30.876654 2 ATCAACG 9490 0.0 30.585083 3 TCAACGC 9605 0.0 30.21889 4 CAACGCA 9715 0.0 29.92563 5 AACGCAG 9980 0.0 29.12517 6 TACGTTG 70 0.008387969 27.145424 5 GACCCGT 90 8.953512E-4 26.386093 6 ACGCAGA 11405 0.0 25.40487 7 CGCAGAG 11520 0.0 24.82141 8 ACTTGAC 515 0.0 23.979965 8 GCAGAGT 12040 0.0 23.512682 9 GAGTACT 6860 0.0 23.160845 12-13 GTACATG 7055 0.0 23.109636 1 AGTACTT 6940 0.0 22.688536 12-13 GTAAGGT 235 4.645699E-9 22.236145 4 TACATGG 7350 0.0 21.780972 2 GTACTTT 7240 0.0 21.582659 14-15 AGAGTAC 10975 0.0 21.185104 10-11 >>END_MODULE