FastQCFastQC Report
Thu 26 May 2016
SRR937480_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937480_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759890
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89450.5082704032638403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61910.35178335009574463No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42840.2434243049281489No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26510.15063441465091568No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45850.048.503551
GTATCAA85500.043.128331
TATCAAC110050.033.2396472
ATCAACG111350.032.850643
TCAACGC112350.032.6042444
CAACGCA114900.031.8374885
AACGCAG117350.031.169246
ACGCAGA134900.026.9037237
GTACATG98400.026.8984431
CGCAGAG135650.026.4062828
TACATGG102300.025.7734812
GCAGAGT142850.024.7413659
ACATGGG101800.024.4993673
GAGTACT74950.022.05597312-13
TGGTATC24600.021.8222262
GTGGTAT25850.021.6914461
CATGGGG40550.021.4413974
GTATAGG5350.021.3169231
CATGGGA63150.021.216254
GTACTTT77550.021.16276714-15
Produced by FastQC (version 0.11.5)