FastQCFastQC Report
Thu 26 May 2016
SRR937480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759890
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106790.6067992885919006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82810.4705407724346408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55950.3179175971225474No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.11188199262453903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55250.047.999161
GTATCAA99800.041.7162551
ATCAACG128850.032.070623
TATCAAC129950.031.7991542
GCGTATC902.4046562E-531.6840361
TCAACGC130850.031.5441364
CAACGCA133800.030.7776585
AACGCAG137200.030.0495666
ACGCAGA156800.026.1463647
CGCAGAG158250.025.7867168
GTACATG98800.025.7352621
TACATGG104000.024.1598872
GCAGAGT165350.024.1048479
ACATGGG104250.023.0084763
GAGTACT91050.022.80069512-13
CGTATCA1302.9433315E-421.9220162
AGTACTT93900.021.65333412-13
GTACTTT96750.021.42919314-15
CCCTATA3600.021.1100882
AGAGTAC149350.020.91400110-11
Produced by FastQC (version 0.11.5)