##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937480_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1759890 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.098252163487494 31.0 31.0 34.0 30.0 34.0 2 32.30768173010813 34.0 31.0 34.0 31.0 34.0 3 32.35996738432516 34.0 31.0 34.0 30.0 34.0 4 36.03380154441471 37.0 35.0 37.0 35.0 37.0 5 35.93913596872532 37.0 35.0 37.0 35.0 37.0 6 35.976858780946536 37.0 35.0 37.0 35.0 37.0 7 35.90648676905943 37.0 35.0 37.0 35.0 37.0 8 35.883696708317 37.0 35.0 37.0 35.0 37.0 9 37.562964162532886 39.0 37.0 39.0 35.0 39.0 10-11 37.479526561319176 39.0 37.0 39.0 35.0 39.0 12-13 37.37349805953781 39.0 37.0 39.0 34.0 39.0 14-15 38.53094000193194 40.0 38.0 41.0 34.0 41.0 16-17 38.499255635295384 40.0 38.0 41.0 34.0 41.0 18-19 38.51541914551478 40.0 38.0 41.0 34.0 41.0 20-21 38.42728579627136 40.0 38.0 41.0 34.0 41.0 22-23 38.270258936638086 40.0 38.0 41.0 34.0 41.0 24-25 38.18655484149578 40.0 37.5 41.0 33.5 41.0 26-27 38.11609049429226 40.0 37.5 41.0 33.0 41.0 28-29 37.97053509026132 40.0 37.0 41.0 33.0 41.0 30-31 38.02642807220906 40.0 37.0 41.0 33.0 41.0 32-33 37.95254049969032 40.0 37.0 41.0 33.0 41.0 34-35 37.81297012881487 40.0 37.0 41.0 33.0 41.0 36-37 37.61834091903471 40.0 37.0 41.0 33.0 41.0 38-39 37.474712908193126 40.0 36.0 41.0 33.0 41.0 40-41 37.27497229940508 40.0 36.0 41.0 32.0 41.0 42-43 37.02482172181216 40.0 36.0 41.0 31.5 41.0 44-45 36.68139429168869 39.0 36.0 41.0 31.0 41.0 46-47 36.253293955872245 39.0 35.0 41.0 30.0 41.0 48-49 35.86936172147123 39.0 35.0 41.0 29.0 41.0 50-51 35.61515236747751 39.0 35.0 40.5 28.0 41.0 52-53 35.35123672502259 39.0 35.0 40.0 27.0 41.0 54-55 35.10400650040627 38.5 35.0 40.0 26.5 41.0 56-57 34.83677729858116 38.0 34.0 40.0 26.0 41.0 58-59 34.549360187284435 38.0 34.0 40.0 26.0 41.0 60-61 34.222165874003494 37.5 34.0 40.0 25.0 41.0 62-63 33.851203484308684 37.0 33.0 40.0 23.5 41.0 64-65 33.45632511122854 36.5 33.0 39.5 22.5 41.0 66-67 33.0413395155379 36.0 32.5 39.0 22.0 40.5 68-69 32.60035286296302 35.5 32.0 39.0 20.5 40.0 70-71 32.19900647199541 35.0 31.5 38.0 20.0 40.0 72-73 31.738353249350812 35.0 31.0 37.5 18.0 39.5 74-75 31.255445226690306 35.0 31.0 37.0 17.0 39.0 76-77 29.988491894379763 33.5 29.5 35.5 13.5 38.0 78-79 30.596906340737206 34.0 30.5 36.0 13.0 38.0 80-81 30.398465529095567 34.0 30.5 35.5 9.0 37.0 82-83 30.063719891584135 34.0 30.0 35.0 7.0 37.0 84-85 29.70818545477274 34.0 30.0 35.0 4.0 36.5 86-87 29.301655785304764 34.0 29.5 35.0 2.0 36.0 88-89 28.856176522396286 34.0 29.0 35.0 2.0 35.5 90-91 28.359210518839244 34.0 29.0 35.0 2.0 35.0 92-93 28.006250674758082 33.0 28.0 35.0 2.0 35.0 94-95 27.567203063827854 33.0 27.0 35.0 2.0 35.0 96-97 27.082497769746972 33.0 26.5 35.0 2.0 35.0 98-99 26.329962668121304 33.0 25.0 35.0 2.0 35.0 100-101 23.933643295887812 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.13760404978387086 1101 2 -0.0667077913360643 1101 3 -0.046117920105125165 1101 4 -0.07211105420666541 1101 5 -0.062374211604144136 1101 6 -0.16137095655538758 1101 7 -0.1633287605331617 1101 8 -0.1629042205405895 1101 9 -0.1328375892382212 1101 10-11 -0.12885776743949862 1101 12-13 -0.14051140084923475 1101 14-15 -0.3206870734701397 1101 16-17 -0.1559571085832374 1101 18-19 -0.19176650736313405 1101 20-21 -0.14768176201888394 1101 22-23 -0.18434043646654885 1101 24-25 -0.15190220234663343 1101 26-27 -0.22329502557970216 1101 28-29 -0.36270056228826064 1101 30-31 -0.21700429817894928 1101 32-33 -0.10156078500649812 1101 34-35 -0.07970712729473917 1101 36-37 -0.13018905833872196 1101 38-39 -0.19103709636470256 1101 40-41 -0.05224915407714548 1101 42-43 -0.07377294287009306 1101 44-45 -0.13530747264142917 1101 46-47 -0.0026939775642631503 1101 48-49 0.0915562841377664 1101 50-51 0.15702450553622072 1101 52-53 0.12454238128480455 1101 54-55 0.08334989630963463 1101 56-57 0.16486774653844805 1101 58-59 0.21775658736769543 1101 60-61 -6.307744636657731E-4 1101 62-63 0.07501694333138431 1101 64-65 0.060136671153451005 1101 66-67 0.1533278133838607 1101 68-69 0.1219971761178229 1101 70-71 0.19437811396439741 1101 72-73 0.11203082739347536 1101 74-75 0.022039367301253776 1101 76-77 0.05818671642103368 1101 78-79 -0.13427006851196666 1101 80-81 -0.07773251641055623 1101 82-83 0.012344583182212432 1101 84-85 0.00792773287026094 1101 86-87 -0.14562726054461805 1101 88-89 -0.20271072825530823 1101 90-91 -0.10655990107159496 1101 92-93 -0.15980429657334128 1101 94-95 -0.20436976165462184 1101 96-97 -0.2038160222572607 1101 98-99 -0.13261878117717885 1101 100-101 0.425584154285918 1102 1 -0.05176270831497476 1102 2 -0.020607915768245277 1102 3 -0.025230085225139476 1102 4 -0.003089608394631682 1102 5 -0.033320046124330815 1102 6 -0.13780052119265918 1102 7 -0.11380325193534446 1102 8 -0.1190629577787945 1102 9 -0.16374511963415728 1102 10-11 -0.18726031305213553 1102 12-13 -0.17591577733905694 1102 14-15 -0.19103937209239064 1102 16-17 -0.1893982581567144 1102 18-19 -0.22203899323333331 1102 20-21 -0.06629065142415413 1102 22-23 -0.1578255121003238 1102 24-25 -0.2337934310020202 1102 26-27 -0.2500371698610735 1102 28-29 -0.26420588251374255 1102 30-31 -0.13216576955204573 1102 32-33 -0.124304093028627 1102 34-35 -0.15948989568596517 1102 36-37 -0.26081122863091366 1102 38-39 0.0019438227843622258 1102 40-41 -0.007133820059188167 1102 42-43 0.0727799201945416 1102 44-45 0.06390114616132792 1102 46-47 0.12734913331530606 1102 48-49 0.1615116740752569 1102 50-51 0.2237313534291232 1102 52-53 0.3324160432640255 1102 54-55 0.28309447301533197 1102 56-57 0.20470361411561555 1102 58-59 0.32598588365122083 1102 60-61 0.3063203746600607 1102 62-63 0.1986981308341953 1102 64-65 0.21989569623478644 1102 66-67 0.22044951330740759 1102 68-69 0.2948674930451034 1102 70-71 0.3132256077053057 1102 72-73 0.4216390440404041 1102 74-75 0.4400136806754631 1102 76-77 0.5039992173580217 1102 78-79 0.37869528724414536 1102 80-81 0.29242559117081157 1102 82-83 0.4456321133532697 1102 84-85 0.29013088527720043 1102 86-87 0.41074927986585763 1102 88-89 0.5208853806153577 1102 90-91 0.38965021129216737 1102 92-93 0.3392842470453843 1102 94-95 0.39597125561596513 1102 96-97 0.33964130912213975 1102 98-99 0.5169858561704679 1102 100-101 1.1447834406748392 1103 1 -0.1267216279000607 1103 2 -0.013213441084054978 1103 3 -0.06599918761806833 1103 4 -0.1014322704055175 1103 5 -0.052653516392375366 1103 6 -0.0938733619033485 1103 7 -0.1679523738414801 1103 8 -0.1549986926047211 1103 9 -0.15459188968653592 1103 10-11 -0.137758413082949 1103 12-13 -0.10941204158882556 1103 14-15 -0.22228455798126845 1103 16-17 -0.24271293413494277 1103 18-19 -0.20952390901847906 1103 20-21 -0.26097009581154396 1103 22-23 -0.11843966430325992 1103 24-25 -0.12305764225805405 1103 26-27 -0.1675971215915837 1103 28-29 -0.21563952480602921 1103 30-31 -0.3658635845874798 1103 32-33 -0.2949338429516004 1103 34-35 -0.23551433853283754 1103 36-37 -0.2323774836375847 1103 38-39 -0.14270737881776796 1103 40-41 -0.15997061527331624 1103 42-43 -0.062013586532380316 1103 44-45 -0.21808380813671135 1103 46-47 0.0019399338698917745 1103 48-49 -0.14260053964559916 1103 50-51 0.09785037109369199 1103 52-53 0.015164515586100435 1103 54-55 0.02248417210129361 1103 56-57 -0.04037475961345649 1103 58-59 -0.062265759565370615 1103 60-61 0.004693846982128491 1103 62-63 0.04758960151304592 1103 64-65 -0.1552166330247431 1103 66-67 0.1066663922228841 1103 68-69 0.10240253977407576 1103 70-71 0.17456476504329288 1103 72-73 0.20013055836719928 1103 74-75 0.3292137037831999 1103 76-77 0.4584545167315497 1103 78-79 0.2853609055043904 1103 80-81 0.15645262721887931 1103 82-83 0.21372835706283055 1103 84-85 0.14741977045744292 1103 86-87 0.16242940464761801 1103 88-89 0.09863327524409371 1103 90-91 0.111530988225514 1103 92-93 0.21666723205103366 1103 94-95 0.2512189576802548 1103 96-97 0.4229302456528963 1103 98-99 0.5772537133133255 1103 100-101 1.130585022270811 1104 1 0.045250992270155166 1104 2 0.04842305326272367 1104 3 0.025136512462886174 1104 4 -0.010130781507236009 1104 5 0.019491428115713916 1104 6 0.03052133552205305 1104 7 -0.0043912353804742565 1104 8 -0.04313085416812612 1104 9 0.01915412397936933 1104 10-11 -0.010280150357687035 1104 12-13 -0.0609124770678946 1104 14-15 -0.004488869024029896 1104 16-17 -0.14954168615729202 1104 18-19 -0.058535978391027754 1104 20-21 0.02584489160424397 1104 22-23 -0.12956091955001625 1104 24-25 -0.05520906893551114 1104 26-27 -0.24995421709955679 1104 28-29 -0.06373001909144449 1104 30-31 -0.03721206254476783 1104 32-33 -0.10669357854311556 1104 34-35 -0.11148076196254664 1104 36-37 -0.12013538661578593 1104 38-39 -0.0015357333737640033 1104 40-41 0.002427600662947782 1104 42-43 0.12542147506823653 1104 44-45 0.17735733283180366 1104 46-47 0.29962462568008874 1104 48-49 0.44199770590056886 1104 50-51 0.3440436304378238 1104 52-53 0.3369405366278144 1104 54-55 0.4355580808360813 1104 56-57 0.3817413085640311 1104 58-59 0.413884592085644 1104 60-61 0.5430889632143661 1104 62-63 0.5334553379128337 1104 64-65 0.5148534170853623 1104 66-67 0.5801763935699995 1104 68-69 0.5138601075734215 1104 70-71 0.5679659566420838 1104 72-73 0.6691134446398053 1104 74-75 0.7061667240606511 1104 76-77 0.5291481755426979 1104 78-79 0.47356782542025755 1104 80-81 0.4049371867181222 1104 82-83 0.49511432756762375 1104 84-85 0.48310978138109917 1104 86-87 0.5931191421464312 1104 88-89 0.5513433155375758 1104 90-91 0.45526629164038823 1104 92-93 0.582226572576996 1104 94-95 0.5775365653062785 1104 96-97 0.5286295530210943 1104 98-99 0.5675990822301245 1104 100-101 1.2018192619963735 1105 1 0.0591413597617958 1105 2 0.05421837214996117 1105 3 0.04422892025406355 1105 4 0.049198110849914656 1105 5 -0.04811235687764537 1105 6 -0.05530354393593484 1105 7 -0.09336338539520028 1105 8 0.03337417681363064 1105 9 0.04682529880534503 1105 10-11 -0.0026357454192122987 1105 12-13 -0.04971098456914547 1105 14-15 0.03146753430204541 1105 16-17 -0.037124696064211093 1105 18-19 0.05730218265206588 1105 20-21 -0.038886040018851986 1105 22-23 -0.13965078988660906 1105 24-25 -0.17136660817303806 1105 26-27 0.005597950589503853 1105 28-29 -0.07501419850137125 1105 30-31 -0.19205674035005416 1105 32-33 -0.10679848756166876 1105 34-35 -0.16112753381385403 1105 36-37 -0.17487205464334465 1105 38-39 -0.12323122848748369 1105 40-41 -0.12380740587448003 1105 42-43 -0.007293601339640077 1105 44-45 -0.08715757923583567 1105 46-47 -0.09312890014147968 1105 48-49 0.0766543743415582 1105 50-51 -0.007084270709974305 1105 52-53 0.04636839488177458 1105 54-55 0.252360061187602 1105 56-57 0.11062694739793244 1105 58-59 0.14883686623409886 1105 60-61 0.19180240655574465 1105 62-63 0.17491507197104994 1105 64-65 0.1996901879547579 1105 66-67 0.3057664422002375 1105 68-69 0.30874437173743274 1105 70-71 0.3573437893147826 1105 72-73 0.2759614712649885 1105 74-75 0.43979032290510034 1105 76-77 0.5386545863622771 1105 78-79 0.2417922790406486 1105 80-81 0.2243918665388982 1105 82-83 0.1953877111677116 1105 84-85 0.12461034210171462 1105 86-87 0.2610598763028449 1105 88-89 0.4350901695590004 1105 90-91 0.6545195254738054 1105 92-93 0.6491771309720455 1105 94-95 0.6590183147546007 1105 96-97 0.7825052435860052 1105 98-99 0.9190685836797243 1105 100-101 1.1440717568155208 1106 1 -0.24033222337702398 1106 2 -0.10924316111990606 1106 3 -0.0827507672793999 1106 4 -0.06414826366970772 1106 5 -0.12538345535191553 1106 6 -0.21665465834903586 1106 7 -0.22094113892397615 1106 8 -0.26435128263801744 1106 9 -0.2547952386693524 1106 10-11 -0.3591083654972067 1106 12-13 -0.33849488044987197 1106 14-15 -0.30509252975561907 1106 16-17 -0.21060279988364528 1106 18-19 -0.20653715986769328 1106 20-21 -0.23623063059920923 1106 22-23 -0.22154164671197663 1106 24-25 -0.24856418574479022 1106 26-27 -0.30379078564827466 1106 28-29 -0.4452039112263222 1106 30-31 -0.4227278971011117 1106 32-33 -0.3868899990380399 1106 34-35 -0.4028882883380902 1106 36-37 -0.5213703989382168 1106 38-39 -0.5667274002711835 1106 40-41 -0.38546964778867476 1106 42-43 -0.47093809996938063 1106 44-45 -0.4168801993588289 1106 46-47 -0.39195735310871527 1106 48-49 -0.4068951212438563 1106 50-51 -0.2553794766611901 1106 52-53 -0.21347067263201325 1106 54-55 -0.28721488180894994 1106 56-57 -0.35438880155328434 1106 58-59 -0.327007422793578 1106 60-61 -0.21528706019363852 1106 62-63 -0.2173601578920028 1106 64-65 -0.2432355444151142 1106 66-67 -0.22928188092026147 1106 68-69 -0.12408559988188017 1106 70-71 -0.1395207286697655 1106 72-73 -0.13760620387088807 1106 74-75 -0.11195417423082432 1106 76-77 -0.007472985811940447 1106 78-79 -0.09017376348124984 1106 80-81 -0.19528803227042246 1106 82-83 -0.2297132968335589 1106 84-85 -0.2774704344229981 1106 86-87 -0.2462157234144442 1106 88-89 -0.20742222595717763 1106 90-91 -0.025218113790710817 1106 92-93 0.13971741151772932 1106 94-95 -0.06334345020060539 1106 96-97 -0.019240558388457174 1106 98-99 0.14059957143530255 1106 100-101 0.7283951026447077 1107 1 0.0288004784982121 1107 2 -0.0062535201638524995 1107 3 0.05444024444518902 1107 4 0.025481820176793235 1107 5 0.04003160222732305 1107 6 -0.056207114599253316 1107 7 -0.013185120067213063 1107 8 -0.013019001734839719 1107 9 -0.050008855897893056 1107 10-11 -0.0011682800851033903 1107 12-13 -0.052941876240154784 1107 14-15 0.052126836698818124 1107 16-17 0.07052619239273383 1107 18-19 0.10725358918424632 1107 20-21 -0.0018332338417366145 1107 22-23 0.014221191196376992 1107 24-25 0.07747030526171983 1107 26-27 0.006659258710357108 1107 28-29 -0.052804783612636186 1107 30-31 0.06103477989850603 1107 32-33 0.06803793993840657 1107 34-35 0.17392356585249757 1107 36-37 0.058110474665795664 1107 38-39 0.05396992168546433 1107 40-41 0.06717936675399727 1107 42-43 0.24077442568904672 1107 44-45 0.2721772450624229 1107 46-47 0.2026925399087176 1107 48-49 0.24138804770162636 1107 50-51 0.26686322156098896 1107 52-53 0.2622237355717232 1107 54-55 0.38948183565269545 1107 56-57 0.29313081191781976 1107 58-59 0.086494125409466 1107 60-61 0.22026268235237012 1107 62-63 0.29516104292211054 1107 64-65 0.3095591577732506 1107 66-67 0.3390621506700384 1107 68-69 0.4886174930450977 1107 70-71 0.4916253329800284 1107 72-73 0.6196129451393091 1107 74-75 0.5241139554007397 1107 76-77 0.5536558107646172 1107 78-79 0.505377979551838 1107 80-81 0.4075354812807035 1107 82-83 0.4393821133532718 1107 84-85 0.37155258857390194 1107 86-87 0.49594845569003 1107 88-89 0.6755900509450292 1107 90-91 0.7490938926108477 1107 92-93 0.8440575986937375 1107 94-95 0.7388627391324469 1107 96-97 0.9613446058254347 1107 98-99 1.1298979440825576 1107 100-101 1.6793988252902246 1108 1 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-0.6741117399616634 2316 90-91 -0.6018312990359931 2316 92-93 -0.5586254570821048 2316 94-95 -0.23068854291883056 2316 96-97 -0.11041309309076652 2316 98-99 -0.3117893741833697 2316 100-101 -1.0207597789920975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 17.0 5 58.0 6 104.0 7 256.0 8 604.0 9 1101.0 10 1924.0 11 3016.0 12 4609.0 13 6412.0 14 8359.0 15 11281.0 16 16127.0 17 22167.0 18 19175.0 19 11052.0 20 9571.0 21 9685.0 22 10313.0 23 11200.0 24 12833.0 25 15189.0 26 18675.0 27 22583.0 28 27808.0 29 34489.0 30 43552.0 31 54005.0 32 67551.0 33 88586.0 34 119897.0 35 167644.0 36 249785.0 37 369387.0 38 295880.0 39 24989.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75783018336903 20.145405710588392 15.183735756648128 21.913028349394445 2 15.750302575729163 21.39332571922109 39.241884435959065 23.61448726909068 3 18.84509827318753 26.091119331321842 30.31570154952866 24.748080845961965 4 12.94251345254533 17.579791918813108 37.113569598099886 32.36412503054168 5 13.400486631277367 38.33800985763592 33.54886257610006 14.71264093498665 6 30.957275738824585 36.832699771008414 17.541437248918967 14.668587241248032 7 28.06743603293388 33.94433743018029 20.729988806118566 17.258237730767263 8 27.11055804624153 34.23316229991647 19.796350908295405 18.859928745546597 9 27.217723835012414 16.5243850467927 20.56537624510623 35.69251487308866 10-11 24.8499761632423 27.342522559687072 27.013751451087487 20.793749825983145 12-13 25.93516535408909 24.804704481099506 28.08232216842097 21.177807996390435 14-15 22.88995334935706 25.71851081601691 26.858667303070078 24.53286853155595 16-17 21.57342220252402 29.29697310627369 27.293864957468934 21.83573973373336 18-19 21.5495286637233 27.133343561245304 30.59208245969919 20.725045315332206 20-21 22.429981419293252 26.2237696674224 30.583814897522004 20.76243401576235 22-23 22.188608742888704 26.251707506665245 30.52161061347504 21.038073136971015 24-25 21.92980312851054 26.45985888802582 30.685291431967766 20.92504655149587 26-27 21.69711185509954 26.758865925254167 30.505789017912544 21.03823320173375 28-29 21.350376525171995 26.838140344945643 30.768435243494803 21.043047886387562 30-31 21.60030342891723 26.442580054946774 30.75968032002091 21.19743619611509 32-33 21.415685071226044 26.719482467654228 30.50517361880572 21.35965884231401 34-35 21.900999494286573 26.62339691685276 30.263510787606045 21.212092801254624 36-37 22.138214398749014 26.43658207989254 30.132184472998013 21.29301904836044 38-39 21.825651848051088 26.7351315230973 30.240112825810385 21.19910380304123 40-41 21.708477554117774 26.707828365262916 30.31498870525036 21.268705375368953 42-43 21.83617984845738 26.698290613348092 29.87826466481439 21.587264873380136 44-45 21.809228769103488 26.928986390043004 29.611060476575783 21.650724364277725 46-47 22.03109285239418 27.220081936939238 29.054031786077537 21.694793424589037 48-49 22.24917466432561 27.374154066447336 28.591815397553255 21.7848558716738 50-51 22.20681406224253 27.421088818051125 28.386461653853367 21.98563546585298 52-53 22.43125990828972 27.696390115291297 28.15749848001864 21.714851496400343 54-55 22.089448772366456 27.580445368744638 28.162612436004526 22.167493422884384 56-57 22.148969537868844 27.910238708101076 28.048798504451984 21.8919932495781 58-59 22.319519969998126 27.242895862809608 28.332708294268393 22.104875872923877 60-61 22.284546193227985 27.41409974487042 28.629147276250222 21.672206785651376 62-63 22.098767536607404 27.357590531226382 28.7656046684736 21.778037263692617 64-65 21.975016045041464 27.24733569920157 28.64421317361118 22.133435082145784 66-67 22.005324196398636 27.58266141633852 28.2850348601333 22.126979527129535 68-69 21.890402241049156 27.394070083925698 28.542238435356754 22.17328923966839 70-71 21.93114342373671 27.591184676314995 28.386205956054074 22.09146594389422 72-73 22.03555335844854 27.506662348214945 28.06982254572729 22.387961747609225 74-75 21.770565205779906 27.77974759786123 27.87338981413611 22.57629738222275 76-77 21.800396615697572 27.65604668473598 28.18329554688077 22.36026115268568 78-79 21.79735665297263 27.501321105296356 28.010869997556664 22.690452244174352 80-81 21.756706884153807 27.835611269912114 27.86072648311158 22.546955362822494 82-83 21.867583848589977 27.636147506354824 27.847922888077537 22.648345756977665 84-85 21.584921463350607 27.424896825293104 27.769494380011352 23.220687331344937 86-87 21.648228014250893 27.122945184074005 27.98933456068277 23.239492240992334 88-89 21.65729108069254 26.956514327600022 28.005727630704193 23.380466961003243 90-91 21.467741376380822 27.196145319938104 28.1997077962484 23.13640550743267 92-93 21.42059179344451 27.0053703837344 28.144623621392206 23.429414201428884 94-95 21.163084056389888 26.96770820903579 28.110705782747786 23.758501951826535 96-97 21.37218803447943 27.061179960111144 27.90842041263943 23.658211592770005 98-99 21.158647595756094 27.202071950033186 27.685795914285453 23.953484539925267 100-101 20.937833069600696 26.953494497700852 27.944644276338764 24.164028156359688 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 539.0 1 400.5 2 317.5 3 410.0 4 373.0 5 384.0 6 724.0 7 1452.5 8 2296.5 9 2711.0 10 2540.0 11 2291.0 12 2395.5 13 2654.0 14 2892.0 15 3147.5 16 3390.0 17 3554.5 18 3656.0 19 3877.0 20 4238.5 21 4767.0 22 5419.5 23 6252.5 24 7168.0 25 8478.5 26 10551.5 27 13068.5 28 15067.0 29 17701.0 30 21187.0 31 23947.0 32 27520.0 33 31984.0 34 37062.5 35 40974.5 36 46218.0 37 51265.5 38 52932.0 39 53452.5 40 53397.5 41 55376.0 42 58382.5 43 61994.0 44 65243.0 45 67408.0 46 66586.5 47 64884.0 48 67101.0 49 66297.5 50 62413.5 51 59198.5 52 56121.5 53 54935.5 54 53522.5 55 49813.0 56 44137.0 57 38661.0 58 35254.5 59 31836.5 60 27329.5 61 22593.0 62 18573.5 63 14655.5 64 10861.0 65 8315.0 66 6394.5 67 5287.0 68 3954.5 69 2438.0 70 1750.5 71 1405.5 72 1184.5 73 969.0 74 790.5 75 639.5 76 429.5 77 267.0 78 175.0 79 114.5 80 64.0 81 46.5 82 40.5 83 25.0 84 12.0 85 8.0 86 5.5 87 2.5 88 1.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06176522396286132 2 0.0 3 0.0 4 0.0 5 0.0020455823943541927 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.7046519952951605E-4 12-13 0.006875429714357148 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.95504264471075E-4 24-25 6.250390649415588E-4 26-27 3.409303990590321E-4 28-29 0.0026990323258840043 30-31 2.8410866588252673E-4 32-33 0.0 34-35 0.0 36-37 5.682173317650535E-5 38-39 5.113955985885482E-4 40-41 8.523259976475803E-5 42-43 0.014375898493655854 44-45 1.136434663530107E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.8410866588252676E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.8410866588252676E-5 82-83 3.693412656472848E-4 84-85 6.250390649415588E-4 86-87 0.0 88-89 0.0 90-91 0.009034655575064351 92-93 0.0030115518583547834 94-95 0.0 96-97 0.0 98-99 1.136434663530107E-4 100-101 0.01230190523271341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1759890.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.73570404367613 #Duplication Level Percentage of deduplicated Percentage of total 1 70.43279041970528 30.80427676766498 2 14.623427828629234 12.791318232339712 3 5.5921260373341575 7.337267080313461 4 2.892093235525291 5.0595093526260735 5 1.6172608816324423 3.5366021640245617 6 1.0046749785116726 2.6364100512163917 7 0.6829347963299256 2.0908043893389703 8 0.47744980631829975 1.6705282739878116 9 0.35983481616296054 1.4163866121882465 >10 2.0304185694964825 16.603842472110742 >50 0.1799059813736178 5.426252531076952 >100 0.10280471121065704 7.913531900420241 >500 0.0033704964249795183 0.990375363101377 >1k 5.185379115353106E-4 0.3307109854001793 >5k 2.592689557676553E-4 0.7867213498050554 >10k+ 1.2963447788382764E-4 0.6054624743851388 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10679 0.6067992885919006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8281 0.4705407724346408 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5595 0.3179175971225474 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1969 0.11188199262453903 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.8410866588252676E-5 0.0 0.0 0.0 0.0 14-15 1.136434663530107E-4 0.0 0.0 0.0 0.0 16-17 1.420543329412634E-4 0.0 0.0 1.136434663530107E-4 0.0 18-19 1.9887606611776872E-4 0.0 0.0 1.136434663530107E-4 0.0 20-21 2.272869327060214E-4 0.0 0.0 1.136434663530107E-4 0.0 22-23 2.272869327060214E-4 0.0 0.0 3.409303990590321E-4 0.0 24-25 2.8410866588252673E-4 0.0 0.0 5.966281983533062E-4 0.0 26-27 2.8410866588252673E-4 0.0 0.0 9.943803305888436E-4 0.0 28-29 2.8410866588252673E-4 0.0 0.0 0.0024149236600014773 0.0 30-31 2.8410866588252673E-4 0.0 0.0 0.005937871116944809 0.0 32-33 2.8410866588252673E-4 0.0 0.0 0.010256322838359215 0.0 34-35 2.8410866588252673E-4 0.0 0.0 0.015057759291773918 0.0 36-37 2.8410866588252673E-4 0.0 0.0 0.021194506474836495 0.0 38-39 3.9775213223553743E-4 0.0 0.0 0.03125195324707794 0.0 40-41 3.9775213223553743E-4 0.0 0.0 0.04613924733932234 0.0 42-43 5.398064651768008E-4 0.0 0.0 0.06912363840921876 0.0 44-45 6.818607981180642E-4 0.0 0.0 0.08838620595605406 0.0 46-47 7.102716647063168E-4 0.0 0.0 0.12952514077584396 0.0 48-49 7.386825312945696E-4 0.0 0.0 0.17762473790975572 0.0 50-51 7.386825312945696E-4 0.0 0.0 0.20893351289001016 0.0 52-53 7.670933978828222E-4 0.0 0.0 0.2643347027371029 0.0 54-55 7.955042644710749E-4 0.0 0.0 0.34539090511338777 0.0 56-57 7.955042644710749E-4 0.0 0.0 0.46437561438499 0.0 58-59 7.955042644710749E-4 0.0 0.0 0.6182772786935548 0.0 60-61 7.955042644710749E-4 0.0 0.0 0.7954474427378984 0.0 62-63 7.955042644710749E-4 0.0 0.0 0.9809419906926001 0.0 64-65 7.955042644710749E-4 0.0 0.0 1.1876310451221384 0.0 66-67 8.523259976475802E-4 0.0 0.0 1.4393797339606453 0.0 68-69 8.523259976475802E-4 0.0 0.0 1.748319497241305 0.0 70-71 9.659694640005909E-4 0.0 0.0 1.9845274420560375 0.0 72-73 0.0010796129303536016 0.0 0.0 2.2654256800140917 0.0 74-75 0.0010796129303536016 0.0 0.0 2.584366068333816 0.0 76-77 0.0010796129303536016 0.0 0.0 2.929529686514498 0.0 78-79 0.0010796129303536016 0.0 0.0 3.325406701555211 0.0 80-81 0.001136434663530107 0.0 0.0 3.832256561489639 0.0 82-83 0.0011932563967066124 0.0 0.0 4.342004329816068 0.0 84-85 0.0012784889964713704 0.0 0.0 4.995028098347055 0.0 86-87 0.0013637215962361284 0.0 0.0 5.76240560489576 0.0 88-89 0.0013637215962361284 0.0 0.0 6.6861565211462075 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5525 0.0 47.99916 1 GTATCAA 9980 0.0 41.716255 1 ATCAACG 12885 0.0 32.07062 3 TATCAAC 12995 0.0 31.799154 2 GCGTATC 90 2.4046562E-5 31.684036 1 TCAACGC 13085 0.0 31.544136 4 CAACGCA 13380 0.0 30.777658 5 AACGCAG 13720 0.0 30.049566 6 ACGCAGA 15680 0.0 26.146364 7 CGCAGAG 15825 0.0 25.786716 8 GTACATG 9880 0.0 25.735262 1 TACATGG 10400 0.0 24.159887 2 GCAGAGT 16535 0.0 24.104847 9 ACATGGG 10425 0.0 23.008476 3 GAGTACT 9105 0.0 22.800695 12-13 CGTATCA 130 2.9433315E-4 21.922016 2 AGTACTT 9390 0.0 21.653334 12-13 GTACTTT 9675 0.0 21.429193 14-15 CCCTATA 360 0.0 21.110088 2 AGAGTAC 14935 0.0 20.914001 10-11 >>END_MODULE