FastQCFastQC Report
Thu 26 May 2016
SRR937479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019962
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64030.31698616112580336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51050.2527275265574303No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33490.16579519812748952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.163517927564974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29150.053.9793431
GTATCAA59650.045.42581
ATCAACG71400.037.662443
TCAACGC73500.036.653744
CAACGCA74600.036.307055
TATCAAC74800.036.204582
AACGCAG77650.034.934346
ACGCAGA91050.029.3763037
CGCAGAG91400.028.951948
GCAGAGT99450.025.557469
TGGTATC14850.024.9550482
GTACATG68000.023.2794911
TACATGG67000.022.6916162
GTGGTAT17750.022.4967161
GAGTACT60800.021.76049412-13
AGAGTAC92200.021.12568910-11
ACATGGG67850.020.9368633
CAGAGTA96850.020.5038110-11
GTACTTT64150.020.4372214-15
TAGACCG1404.8413425E-420.3647045
Produced by FastQC (version 0.11.5)