##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937479_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2019962 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51731864262793 31.0 31.0 34.0 30.0 34.0 2 31.634102522720724 33.0 31.0 34.0 30.0 34.0 3 31.671932937352288 33.0 31.0 34.0 30.0 34.0 4 35.31810548911316 37.0 35.0 37.0 33.0 37.0 5 35.32138822413491 37.0 35.0 37.0 33.0 37.0 6 35.34656394526234 37.0 35.0 37.0 33.0 37.0 7 35.27619281946888 37.0 35.0 37.0 33.0 37.0 8 35.253200802787376 37.0 35.0 37.0 33.0 37.0 9 36.82512591821034 39.0 37.0 39.0 33.0 39.0 10-11 36.68895454468945 39.0 37.0 39.0 32.0 39.0 12-13 36.523854161612945 39.0 37.0 39.0 32.0 39.0 14-15 37.57141470978167 40.0 37.0 41.0 32.0 41.0 16-17 37.463478025824244 40.0 37.0 41.0 32.0 41.0 18-19 37.351299430385325 40.0 37.0 41.0 32.0 41.0 20-21 37.19220262559395 39.0 37.0 41.0 31.5 41.0 22-23 37.27641089287819 39.0 37.0 41.0 32.0 41.0 24-25 37.24171692338767 39.0 37.0 41.0 31.5 41.0 26-27 37.14586611035257 39.0 37.0 41.0 31.0 41.0 28-29 36.98991787964328 39.0 36.0 41.0 31.0 41.0 30-31 36.728564448242096 39.0 36.0 40.5 30.0 41.0 32-33 36.47104277209175 39.0 35.5 40.0 30.0 41.0 34-35 36.45737642589316 39.0 35.5 40.0 30.0 41.0 36-37 36.296119927008526 39.0 35.0 40.0 30.0 41.0 38-39 36.12638703104316 39.0 35.0 40.0 29.5 41.0 40-41 35.86603287586598 38.5 35.0 40.0 28.5 41.0 42-43 35.57168699213153 38.0 35.0 40.0 27.0 41.0 44-45 35.315524004907026 38.0 35.0 40.0 26.5 41.0 46-47 34.92278716134264 38.0 34.0 40.0 25.0 41.0 48-49 34.570387462734445 38.0 34.0 40.0 24.0 41.0 50-51 33.613491243894686 37.5 32.5 39.5 23.0 40.5 52-53 33.735384130988606 37.5 33.0 39.5 23.0 40.0 54-55 33.99163301091802 38.0 33.0 40.0 23.0 41.0 56-57 33.66784399904553 38.0 33.0 40.0 21.5 41.0 58-59 33.34236733166267 37.5 32.5 40.0 19.5 41.0 60-61 32.95833312705883 37.0 32.0 40.0 18.0 41.0 62-63 32.51261533632811 36.0 31.0 39.0 14.5 41.0 64-65 32.016149066170556 36.0 31.0 39.0 10.0 40.0 66-67 31.533090473979215 35.0 30.5 39.0 8.0 40.0 68-69 30.970381125981575 35.0 29.5 38.0 6.5 40.0 70-71 30.440000356442347 35.0 29.0 38.0 2.0 40.0 72-73 29.880933651227103 34.0 29.0 37.0 2.0 39.0 74-75 29.256530073338013 34.0 28.0 36.5 2.0 39.0 76-77 28.652300142279906 34.0 26.5 36.0 2.0 38.5 78-79 28.01833722614584 33.5 26.0 35.5 2.0 37.5 80-81 27.352570988959197 33.0 25.5 35.0 2.0 37.0 82-83 26.70313377182343 32.5 24.5 35.0 2.0 36.5 84-85 26.009863056829783 32.0 22.5 35.0 2.0 36.0 86-87 25.2878618508665 31.5 19.5 34.5 2.0 35.5 88-89 24.605820802569554 31.0 17.5 34.0 2.0 35.0 90-91 23.91944972232151 31.0 9.0 34.0 2.0 35.0 92-93 23.10916591500236 30.0 2.0 34.0 2.0 35.0 94-95 22.199110923868865 29.5 2.0 34.0 2.0 35.0 96-97 21.197278958713085 29.0 2.0 34.0 2.0 35.0 98-99 19.89459875977865 28.0 2.0 34.0 2.0 35.0 100-101 16.947855207177163 21.0 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21371267189251952 1101 2 0.29567657849982965 1101 3 0.25286834761242716 1101 4 0.08747502129793361 1101 5 0.062286175015174194 1101 6 0.2440197434943414 1101 7 0.20814504449617743 1101 8 0.14471904604728536 1101 9 0.11191784204122257 1101 10-11 0.1629894977213482 1101 12-13 0.2605495414758323 1101 14-15 0.36800107906753254 1101 16-17 0.3542353309214974 1101 18-19 0.32605578672190205 1101 20-21 0.4315192356857267 1101 22-23 0.45094418633105704 1101 24-25 0.4327357709625872 1101 26-27 0.43412661998699775 1101 28-29 0.20657205638544696 1101 30-31 0.3856357741501739 1101 32-33 0.24168883603504554 1101 34-35 0.40196952409452535 1101 36-37 0.45898629644192823 1101 38-39 0.4478417101919021 1101 40-41 0.5174597623102883 1101 42-43 0.5696059839666034 1101 44-45 0.48834069449955564 1101 46-47 0.7055127431243235 1101 48-49 0.7933482415122057 1101 50-51 0.7838395528717754 1101 52-53 0.6922619016295002 1101 54-55 0.8446855767707362 1101 56-57 0.8831091213000946 1101 58-59 0.8219770607188508 1101 60-61 0.93221297776131 1101 62-63 0.9759447708883258 1101 64-65 0.7410400438640323 1101 66-67 0.9486512514951748 1101 68-69 0.9042926688632242 1101 70-71 0.7538637333583615 1101 72-73 0.8280474732011989 1101 74-75 1.1137600373027965 1101 76-77 0.9363347475334898 1101 78-79 1.2520060665280148 1101 80-81 1.3052101232461055 1101 82-83 1.1250759306881442 1101 84-85 1.4197900397866867 1101 86-87 1.4487252096031078 1101 88-89 1.5658801646430653 1101 90-91 1.6281113667145704 1101 92-93 1.8981795430322776 1101 94-95 1.9853080920904453 1101 96-97 2.2752029160361325 1101 98-99 2.5138736944664046 1101 100-101 2.472466649773718 1102 1 0.18670440883774475 1102 2 0.17340195389962432 1102 3 0.045544663544554 1102 4 0.09746735454607602 1102 5 0.04733600890221368 1102 6 -0.053911782344165715 1102 7 -0.0213413605716255 1102 8 -0.02449817084435324 1102 9 0.09006798647043723 1102 10-11 0.011256540755830713 1102 12-13 0.10570477384036536 1102 14-15 0.25482201007450556 1102 16-17 0.3704712268085544 1102 18-19 0.43635924295559647 1102 20-21 0.26055396065068237 1102 22-23 0.25799585559202853 1102 24-25 0.2855693714706007 1102 26-27 0.36979037483680344 1102 28-29 0.4126427298979749 1102 30-31 0.4448701444310572 1102 32-33 0.3166526319290668 1102 34-35 0.456851851069878 1102 36-37 0.5323393000274947 1102 38-39 0.4577202037913324 1102 40-41 0.5270712748287707 1102 42-43 0.5505831463391146 1102 44-45 0.5034180589955213 1102 46-47 0.4054285250168448 1102 48-49 0.34237770042537363 1102 50-51 0.33795694710545376 1102 52-53 0.44778602866525574 1102 54-55 0.5150601634243941 1102 56-57 0.518625930072723 1102 58-59 0.6031426006053167 1102 60-61 0.6439260708697532 1102 62-63 0.6186032751747348 1102 64-65 0.4021500578810162 1102 66-67 0.6975893289216266 1102 68-69 0.8581167517570698 1102 70-71 0.8944082663447581 1102 72-73 0.8311405042012048 1102 74-75 0.7940841705339032 1102 76-77 0.7861188781315711 1102 78-79 0.7223932374965862 1102 80-81 0.7295514795642042 1102 82-83 0.8629189016706498 1102 84-85 1.076947417292903 1102 86-87 1.0886163262384123 1102 88-89 1.0869676043351575 1102 90-91 1.4007975952147476 1102 92-93 1.6255794686880165 1102 94-95 1.4584837536381983 1102 96-97 1.3345933318218997 1102 98-99 1.4710053225282635 1102 100-101 1.0936747052841014 1103 1 0.19702428146764817 1103 2 0.2326422070608487 1103 3 0.11713771082149194 1103 4 0.11696366370804157 1103 5 0.11051447177673168 1103 6 0.2565541448390931 1103 7 0.1591689093003481 1103 8 0.14378167494309224 1103 9 0.17444672829599028 1103 10-11 0.1696599408005781 1103 12-13 0.08042417804305302 1103 14-15 0.10095090650735727 1103 16-17 0.0396622687959578 1103 18-19 0.09978902492640884 1103 20-21 0.062336878846977584 1103 22-23 -0.038556072722826684 1103 24-25 0.051963666132380126 1103 26-27 -0.010371864140033438 1103 28-29 -0.05517197819028752 1103 30-31 0.13659635135483228 1103 32-33 0.09643262903429672 1103 34-35 0.3481773948270259 1103 36-37 0.3171785742284712 1103 38-39 0.45221552609820037 1103 40-41 0.4230797749406179 1103 42-43 0.2514594736592102 1103 44-45 0.34906010691720013 1103 46-47 0.25698183492882265 1103 48-49 0.47226756739731 1103 50-51 0.5991084096246979 1103 52-53 0.4575795785803791 1103 54-55 0.6476533554400703 1103 56-57 0.5471045942656687 1103 58-59 0.4837166871064582 1103 60-61 0.4148697674973363 1103 62-63 0.49861353846767287 1103 64-65 0.526824724652954 1103 66-67 0.8212389822065411 1103 68-69 0.6971823973576718 1103 70-71 0.9495372812002181 1103 72-73 0.9568671584992892 1103 74-75 0.9877156656851582 1103 76-77 0.9164640142517833 1103 78-79 0.9995317524770435 1103 80-81 1.03830685792445 1103 82-83 1.2695288570648025 1103 84-85 1.2766908349694503 1103 86-87 1.4444795612809784 1103 88-89 1.522591757284541 1103 90-91 1.4542232981845231 1103 92-93 1.8875658106135695 1103 94-95 1.8511376043467607 1103 96-97 2.005767952540591 1103 98-99 2.0031577192703267 1103 100-101 1.6999247875220256 1104 1 8.646500698112902E-4 1104 2 0.06487769730906834 1104 3 0.04768248888679949 1104 4 0.0036236292106437418 1104 5 0.175106028937428 1104 6 0.09853006678277154 1104 7 0.0774533989966173 1104 8 0.06453195188743166 1104 9 0.18320486800510594 1104 10-11 0.13088921011226518 1104 12-13 0.18356963457815567 1104 14-15 0.3036473100047772 1104 16-17 0.21930738144581596 1104 18-19 0.30523254276811684 1104 20-21 0.17939552424363114 1104 22-23 0.2755261153874784 1104 24-25 0.11470782049855899 1104 26-27 0.10845750880780258 1104 28-29 0.11085308308070552 1104 30-31 0.19522200251771693 1104 32-33 0.21709381137576855 1104 34-35 0.45441864891291317 1104 36-37 0.3907359678164184 1104 38-39 0.33660568295304927 1104 40-41 0.41194008925560155 1104 42-43 0.33838705513749545 1104 44-45 0.2920413141933622 1104 46-47 0.6368065392677877 1104 48-49 0.7630343918003035 1104 50-51 0.7268180859665918 1104 52-53 0.6347907366644421 1104 54-55 0.7612764701379291 1104 56-57 0.7828956382650816 1104 58-59 0.8846148116355437 1104 60-61 0.8770458976359805 1104 62-63 0.8467900055714068 1104 64-65 0.986030358059665 1104 66-67 1.0021912647390359 1104 68-69 0.9361125129020031 1104 70-71 0.9171226606061147 1104 72-73 0.9584500270908372 1104 74-75 0.8951931904274595 1104 76-77 1.0540778068639831 1104 78-79 1.0897206614538426 1104 80-81 1.160112079185577 1104 82-83 1.3212591801766784 1104 84-85 1.2460694554757161 1104 86-87 1.4495974261697917 1104 88-89 1.3015614819124615 1104 90-91 1.3213431638266186 1104 92-93 1.45292781095349 1104 94-95 1.4269362955714051 1104 96-97 1.4503269536328673 1104 98-99 1.4497546579074339 1104 100-101 1.343972841770082 1105 1 0.12087665821912097 1105 2 -0.05609453187062741 1105 3 0.0993142990632414 1105 4 0.13058341465833934 1105 5 0.17561773984554208 1105 6 0.03636688702196267 1105 7 0.19202313720352038 1105 8 0.0918477983533279 1105 9 0.08901289454082217 1105 10-11 0.17639790551697132 1105 12-13 0.16343964564367752 1105 14-15 0.2409294176648089 1105 16-17 0.1556497365117835 1105 18-19 0.225840146504936 1105 20-21 0.3047119994855336 1105 22-23 0.37044489788897295 1105 24-25 0.19281510419162373 1105 26-27 0.3252660989437075 1105 28-29 0.271385233697508 1105 30-31 0.44042866280326365 1105 32-33 0.3413976376626664 1105 34-35 0.39552072338893396 1105 36-37 0.4051055283070255 1105 38-39 0.1896053233190358 1105 40-41 0.3403713039082703 1105 42-43 0.38284602498982423 1105 44-45 0.4111507361198292 1105 46-47 0.28404192682416607 1105 48-49 0.2467006693871312 1105 50-51 0.09001720201741392 1105 52-53 0.150960083170105 1105 54-55 0.4124504157576041 1105 56-57 0.34146238808028073 1105 58-59 0.4881784561734932 1105 60-61 0.57804701962948 1105 62-63 0.34065486110464605 1105 64-65 0.5513341859624887 1105 66-67 0.5153747332089331 1105 68-69 0.2912758824447188 1105 70-71 0.3661119956530463 1105 72-73 0.4713865277171898 1105 74-75 0.48252644224614016 1105 76-77 0.588669643800209 1105 78-79 0.7267537903362253 1105 80-81 0.712856007735656 1105 82-83 0.7968560460640681 1105 84-85 0.7733673471729112 1105 86-87 0.8073668406163073 1105 88-89 0.9080299387054787 1105 90-91 0.9493674875108766 1105 92-93 1.0151196285703818 1105 94-95 0.8354187746796846 1105 96-97 0.8487001797690468 1105 98-99 0.9675846409706992 1105 100-101 0.931259680511662 1106 1 -0.16105981880453157 1106 2 -0.8292719981645718 1106 3 -0.31475255955754733 1106 4 -0.16263717507064257 1106 5 -0.030625663571647976 1106 6 -0.1958952054251455 1106 7 -0.09084035750964858 1106 8 -0.12201691226552924 1106 9 -0.1653115932993856 1106 10-11 -0.6790282760533657 1106 12-13 -0.5826412297495409 1106 14-15 -0.42254310448573307 1106 16-17 -0.31210981004077354 1106 18-19 -0.16827595924058159 1106 20-21 -0.2387874499712126 1106 22-23 -0.287482446593593 1106 24-25 -0.16891141197359616 1106 26-27 -0.25848805405139075 1106 28-29 -0.09726778641498157 1106 30-31 -0.003914302829002736 1106 32-33 -0.08789150279799429 1106 34-35 -0.012901870817991323 1106 36-37 -0.1479874870066098 1106 38-39 0.0573044609975355 1106 40-41 0.10133745580227327 1106 42-43 0.060921283811396165 1106 44-45 0.03174004189067148 1106 46-47 0.09935472077231111 1106 48-49 0.16044073495182687 1106 50-51 -0.0066146398441802035 1106 52-53 -0.030607224423121693 1106 54-55 0.2867170634349492 1106 56-57 -0.04946086118480508 1106 58-59 -0.2533799418928311 1106 60-61 -0.06384106037299375 1106 62-63 0.10959947409775594 1106 64-65 0.12547388852192398 1106 66-67 0.3674631542832607 1106 68-69 0.4357458299959056 1106 70-71 0.43705819771420096 1106 72-73 0.38431862689152396 1106 74-75 0.2254285375490035 1106 76-77 0.29237745695112594 1106 78-79 0.5588479413293364 1106 80-81 0.3992497690795709 1106 82-83 0.35914950034027626 1106 84-85 0.4972515034506486 1106 86-87 0.4397078160494168 1106 88-89 0.6461944547205469 1106 90-91 0.3868946282630006 1106 92-93 0.7262802922546925 1106 94-95 0.7588163478732319 1106 96-97 0.7076030456852678 1106 98-99 0.8336333449123856 1106 100-101 0.7314743040593079 1107 1 -0.016150350087102083 1107 2 -0.001604683184609712 1107 3 -0.009789655869862912 1107 4 0.0970322869019995 1107 5 -0.09328619742712618 1107 6 -0.025081913839699155 1107 7 0.031092146680407495 1107 8 -0.029189641242957975 1107 9 0.03808580094632674 1107 10-11 -0.062041250314173624 1107 12-13 -0.035170996196860926 1107 14-15 -0.027628184703594627 1107 16-17 0.08211754878028188 1107 18-19 0.06616706550433804 1107 20-21 0.021980310687794713 1107 22-23 -0.1133051212784153 1107 24-25 -0.043414910830321674 1107 26-27 -0.12012640425394494 1107 28-29 -0.18001340412236289 1107 30-31 -0.25162276607937883 1107 32-33 -0.3186355258872524 1107 34-35 -0.20036116953488659 1107 36-37 -0.21387214393113396 1107 38-39 -0.034983608874938454 1107 40-41 -0.1865981387780593 1107 42-43 -0.32058210893126926 1107 44-45 -0.28674740757795547 1107 46-47 -0.18528443328059296 1107 48-49 -0.21238501704071666 1107 50-51 -0.292597208792877 1107 52-53 -0.3267682030854715 1107 54-55 -0.25873473170968 1107 56-57 -0.17933066068737702 1107 58-59 -0.23574556587984574 1107 60-61 -0.2622095748779145 1107 62-63 -0.24716562426124966 1107 64-65 -0.3154696399013801 1107 66-67 -0.3052158550475106 1107 68-69 0.0015381822248095034 1107 70-71 0.03400511088528191 1107 72-73 0.12566233614481348 1107 74-75 0.28379154284453634 1107 76-77 0.2119141091833754 1107 78-79 0.22029239716044913 1107 80-81 0.3973483468604435 1107 82-83 0.3585219010909242 1107 84-85 0.4292156746925748 1107 86-87 0.2994981644669359 1107 88-89 0.29233795363542114 1107 90-91 0.2432538040038743 1107 92-93 0.35894585199182316 1107 94-95 0.21693073010770547 1107 96-97 0.07833740585382998 1107 98-99 0.5689499936268803 1107 100-101 0.6890097832224313 1108 1 -0.06726248962104009 1108 2 -0.03233000272900455 1108 3 -0.016497620957164116 1108 4 -0.0862461991930914 1108 5 -0.04208695071715596 1108 6 0.0015294175035265312 1108 7 -0.05073533693400378 1108 8 -0.12700832792324945 1108 9 -0.008100597009843113 1108 10-11 0.012555981423339801 1108 12-13 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3.9080500237991345 2316 1 -0.052800136770418504 2316 2 -0.044513180410117315 2316 3 -0.18513688580271292 2316 4 -0.03860686222366638 2316 5 -0.011723100902102601 2316 6 -0.007908507171265455 2316 7 0.179343168998642 2316 8 0.08785961567951261 2316 9 -0.09870394658822335 2316 10-11 -0.0020730442557876927 2316 12-13 -0.0555861235754449 2316 14-15 -0.19493987932542467 2316 16-17 -0.12431538000785736 2316 18-19 0.21764159911374747 2316 20-21 0.03480729189479348 2316 22-23 0.029298612451910344 2316 24-25 0.11969292475668425 2316 26-27 0.13611032174581794 2316 28-29 0.4041845805242801 2316 30-31 0.20077144681922476 2316 32-33 0.2815044349639848 2316 34-35 0.34219883198108647 2316 36-37 -0.052487985296600925 2316 38-39 0.12559785488402753 2316 40-41 0.1585551800884062 2316 42-43 0.021888207495130985 2316 44-45 0.09687708400173989 2316 46-47 0.2094103487289729 2316 48-49 0.33942359278709944 2316 50-51 0.4429015057405721 2316 52-53 0.42985491790234676 2316 54-55 0.252287296357693 2316 56-57 0.13880372956209186 2316 58-59 0.41596539408815403 2316 60-61 0.3495413332983546 2316 62-63 0.5010869377151721 2316 64-65 0.6530177021837247 2316 66-67 0.7950648957016675 2316 68-69 1.0561023360804818 2316 70-71 1.061940580257481 2316 72-73 1.0834151517207076 2316 74-75 1.2528627706893722 2316 76-77 1.423505929216283 2316 78-79 1.452267515099745 2316 80-81 1.6963299814030357 2316 82-83 1.8200188989054986 2316 84-85 2.155847438492426 2316 86-87 1.9763057009753418 2316 88-89 1.9085152310637632 2316 90-91 2.261082897799554 2316 92-93 2.437142426666874 2316 94-95 2.443064325656671 2316 96-97 2.490717382835939 2316 98-99 2.5470288140592103 2316 100-101 2.727633885446263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9236.0 3 1935.0 4 2019.0 5 2223.0 6 2525.0 7 3062.0 8 3737.0 9 4746.0 10 6117.0 11 7529.0 12 9647.0 13 11920.0 14 13251.0 15 15933.0 16 18946.0 17 22901.0 18 20900.0 19 18340.0 20 19537.0 21 21093.0 22 23779.0 23 26397.0 24 29435.0 25 33640.0 26 38596.0 27 44653.0 28 52075.0 29 61010.0 30 71663.0 31 85820.0 32 104746.0 33 129780.0 34 163284.0 35 207833.0 36 261695.0 37 290694.0 38 166332.0 39 12932.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.913471265098444 19.16381826298939 14.674969124015929 23.247741347896238 2 15.216494579259072 20.17023921150693 40.48125239194134 24.132013817292663 3 19.191376188320508 24.273555947360478 29.550860514799904 26.984207349519114 4 12.675728452199778 16.515173626312535 37.42260303958972 33.386494881897974 5 13.83564506529652 37.098023954384516 34.36186915479612 14.704461825522852 6 31.872909698996654 36.12799341027957 17.73016881196471 14.26892807875907 7 28.449322208669813 32.35668328352906 20.854724690381506 18.33926981741962 8 26.19878682257479 35.14468014156383 19.936790518478524 18.719742517382855 9 25.995046171055485 16.924903982963833 20.382857230768924 36.69719261521176 10-11 24.68099784722093 26.835141791831845 27.534106348526866 20.949754012420357 12-13 25.41457973794337 24.016828596206743 28.17191867584605 22.396672990003832 14-15 22.56062023674215 25.532116549446826 26.24349919277224 25.66376402103878 16-17 22.04498794854905 28.765889245739196 26.891246664607436 22.297876141104318 18-19 21.93162502567522 27.447679604993546 29.137233188559815 21.48346218077142 20-21 22.613657366346924 26.56397572233255 29.318967516897736 21.503399394422786 22-23 22.316231831566622 26.428257790192582 29.164109421165307 22.09140095707549 24-25 21.999740504151312 26.592296049535424 29.33875942626113 22.069204020052133 26-27 21.886883538255177 26.845245493170637 29.1700249736483 22.097845994925887 28-29 21.900887140873685 26.99135288238908 29.205731531461794 21.902028445275445 30-31 22.09894039054751 26.76431388420651 29.150711894470334 21.986033830775646 32-33 21.830166586977985 26.782022134017424 29.147522980487395 22.240288298517193 34-35 22.133815013125442 26.88683833125741 29.077571619267857 21.901775036349292 36-37 22.13547384337907 26.728738480927554 29.027305892232715 22.108481783460665 38-39 21.93913316554868 26.997577491257292 29.030912164089617 22.03237717910441 40-41 22.26273687740654 26.905294678383505 28.91134062249858 21.920627821711374 42-43 21.978336308688 26.87000792876614 28.81578072545729 22.335875037088567 44-45 22.05583823715833 27.035053262969306 28.79916507836569 22.109943421506674 46-47 22.15139970068794 27.174627150064907 28.345204258646906 22.32876889060025 48-49 22.170001972526062 27.227930969031338 28.310413572964233 22.291653485478363 50-51 22.322384818253195 27.357805122320894 28.148595545488565 22.171214513937347 52-53 22.318140643031757 27.42593718589847 27.941082308560933 22.314839862508844 54-55 22.19246699555938 27.30281758810964 28.148389285041702 22.35632613128928 56-57 22.156463526620737 27.544881363970784 28.08238614518111 22.216268964227368 58-59 22.24975036174604 27.323396746989086 28.202376387519923 22.224476503744945 60-61 22.26129855569969 27.365931577420007 28.237869291194055 22.13490057568625 62-63 22.13962983495992 27.341437127382367 28.401404459195152 22.11752857846256 64-65 22.05316488724486 27.222102894117167 28.367239325825416 22.35749289281255 66-67 22.09700510756849 27.381994134431835 28.117100036114124 22.403900721885552 68-69 22.027707424322738 27.377408673967114 28.21218331953824 22.382700582171914 70-71 22.176047513262894 27.178815680726114 28.252547160544783 22.392589645466206 72-73 22.07318851867764 27.271712524420483 28.165509598030685 22.489589358871196 74-75 22.03146100639103 27.539295250436837 27.87449929005109 22.55474445312105 76-77 22.13424722581055 27.31230418051725 27.96844907620465 22.584999517467548 78-79 22.158772483233633 27.27664254347174 27.995302216388307 22.56928275690632 80-81 22.086469103045417 27.485442206166244 27.825665674565276 22.602423016223067 82-83 22.21152582060638 27.36830486150213 27.82126618826383 22.598903129627654 84-85 22.25954012178499 27.255120505235087 27.858737944020174 22.62660142895975 86-87 22.162089765466884 27.234290051192506 27.98585757371324 22.617762609627366 88-89 22.267532961702585 27.074795710991236 27.915938325468858 22.741733001837318 90-91 22.28324458618412 27.158736060678297 27.91572646447679 22.642292888660794 92-93 22.3073610555738 27.058678488754563 27.910929510999367 22.72303094467227 94-95 22.251205265763705 27.05785672542761 27.78166110873536 22.909276900073323 96-97 22.186743163838415 26.98269213452263 27.873524653990287 22.95704004764866 98-99 22.171661479800996 26.925848700637435 27.781191585742054 23.12129823381952 100-101 22.871254614065453 26.048554280161145 28.284438253498408 22.795752852275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3095.0 1 1752.0 2 405.5 3 408.5 4 347.5 5 340.0 6 560.5 7 991.5 8 1582.5 9 2020.5 10 1967.0 11 1785.5 12 1847.0 13 2054.0 14 2242.5 15 2351.0 16 2469.5 17 2610.5 18 2741.5 19 2997.0 20 3402.5 21 3872.5 22 4598.5 23 5747.0 24 7061.5 25 8721.5 26 11118.0 27 14125.5 28 16907.0 29 20076.5 30 24050.0 31 27527.5 32 31155.0 33 35023.0 34 39420.0 35 44149.5 36 49489.5 37 54635.0 38 57723.5 39 60007.0 40 62406.0 41 65593.5 42 68466.0 43 71140.0 44 74080.0 45 76305.5 46 77961.0 47 79427.5 48 79520.5 49 78725.5 50 77219.0 51 73602.5 52 69879.5 53 67368.0 54 65162.0 55 60454.5 56 53737.5 57 47692.0 58 42688.5 59 37357.5 60 31148.0 61 25530.0 62 20378.0 63 15561.5 64 11693.0 65 8815.5 66 6591.0 67 5028.5 68 3894.0 69 2871.0 70 2136.0 71 1587.0 72 1155.5 73 890.5 74 690.0 75 479.0 76 304.0 77 212.5 78 142.0 79 76.5 80 41.0 81 27.0 82 20.5 83 14.5 84 11.0 85 9.5 86 9.5 87 6.5 88 4.0 89 5.0 90 3.0 91 1.5 92 2.0 93 1.5 94 1.0 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30995632591108147 2 0.26658917345969874 3 0.26505449112409046 4 0.26475745583332755 5 0.2664901616961111 6 0.23277665619452245 7 0.23465787970268748 8 0.23554898557497617 9 0.24530164428835788 10-11 0.2536928912524097 12-13 0.24453430312055377 14-15 0.23423707970744004 16-17 0.22993006799137805 18-19 0.21782587989279006 20-21 0.22423689158508922 22-23 0.2201526563370994 24-25 0.22354380923997583 26-27 0.21968235046005816 28-29 0.2338657855939864 30-31 0.24859378542764665 32-33 0.23903915024144018 34-35 0.2372321855559659 36-37 0.2254745386299346 38-39 0.2120336917229136 40-41 0.20943463292873826 42-43 0.22356856218087273 44-45 0.21997938575082104 46-47 0.2313904915042956 48-49 0.23661336203354322 50-51 0.24832150307778064 52-53 0.2617375970439048 54-55 0.2384450796599144 56-57 0.210845550559862 58-59 0.20025129185598542 60-61 0.203320656527202 62-63 0.2098801858648826 64-65 0.20025129185598542 66-67 0.20445929180845976 68-69 0.20537515062164535 70-71 0.2073306329525011 72-73 0.20970691527860424 74-75 0.21406343287646004 76-77 0.2254250327481408 78-79 0.22492997393020264 80-81 0.21658823284794468 82-83 0.2092613623424599 84-85 0.19554823308557287 86-87 0.20010277421060396 88-89 0.2104742564464084 90-91 0.20874155058362484 92-93 0.21287529171340847 94-95 0.20911284469707844 96-97 0.21661298578884158 98-99 0.220523950450553 100-101 0.20371670358155253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2019962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.18304783210004 #Duplication Level Percentage of deduplicated Percentage of total 1 80.73669902622031 50.20454017353321 2 11.298814830031477 14.051894860437772 3 3.3529208976917095 6.25484521675234 4 1.4814080980248459 3.684738824733573 5 0.8099114133497922 2.5181380078046933 6 0.47466231173360063 1.7709569540775398 7 0.33398449215659204 1.45377215556671 8 0.23982850457577543 1.193061389722917 9 0.1847301527452127 1.0338375531768036 >10 0.9972491811051776 11.307039999220308 >50 0.06192311101138711 2.6132649755158583 >100 0.02699277105835692 2.7557832638284445 >500 4.773874340616361E-4 0.19290566102745818 >1k 2.3869371703080441E-4 0.39585488429869814 >5k 1.5912914468720296E-4 0.5693660803036393 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6403 0.31698616112580336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5105 0.2527275265574303 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3349 0.16579519812748952 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3303 0.163517927564974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.9505881793815925E-5 0.0 5 0.0 0.0 0.0 4.9505881793815925E-5 0.0 6 0.0 0.0 0.0 4.9505881793815925E-5 0.0 7 0.0 0.0 0.0 4.9505881793815925E-5 0.0 8 0.0 0.0 0.0 4.9505881793815925E-5 0.0 9 0.0 0.0 0.0 4.9505881793815925E-5 0.0 10-11 0.0 0.0 0.0 4.9505881793815925E-5 0.0 12-13 0.0 0.0 0.0 9.901176358763185E-5 0.0 14-15 0.0 0.0 0.0 9.901176358763185E-5 0.0 16-17 0.0 0.0 0.0 2.2277646807217165E-4 0.0 18-19 0.0 0.0 0.0 2.475294089690796E-4 0.0 20-21 0.0 0.0 0.0 2.475294089690796E-4 0.0 22-23 0.0 0.0 0.0 2.9703529076289555E-4 0.0 24-25 0.0 0.0 0.0 5.198117588350672E-4 0.0 26-27 0.0 0.0 0.0 9.158588131855946E-4 0.0 28-29 0.0 0.0 0.0 0.001930729389958821 0.0 30-31 0.0 0.0 0.0 0.004777317593103236 0.0 32-33 0.0 0.0 0.0 0.008787294018402326 0.0 34-35 0.0 0.0 0.0 0.01297054102997977 0.0 36-37 0.0 0.0 0.0 0.01821816450012426 0.0 38-39 0.0 2.4752940896907962E-5 0.0 0.026337129114310073 0.0 40-41 0.0 4.9505881793815925E-5 0.0 0.0376244701633001 0.0 42-43 0.0 4.9505881793815925E-5 0.0 0.05237722293785725 0.0 44-45 0.0 4.9505881793815925E-5 0.0 0.06844188157995051 0.0 46-47 0.0 4.9505881793815925E-5 0.0 0.08661054019828096 0.0 48-49 4.9505881793815925E-5 4.9505881793815925E-5 0.0 0.10611585762504444 0.0 50-51 4.9505881793815925E-5 4.9505881793815925E-5 0.0 0.13349261025702464 0.0 52-53 4.9505881793815925E-5 7.425882269072389E-5 0.0 0.17606766859970635 0.0 54-55 4.9505881793815925E-5 9.901176358763185E-5 0.0 0.22953402093702752 0.0 56-57 4.9505881793815925E-5 9.901176358763185E-5 0.0 0.2806735968300394 0.0 58-59 4.9505881793815925E-5 9.901176358763185E-5 0.0 0.3495115254643404 0.0 60-61 4.9505881793815925E-5 1.4851764538144777E-4 0.0 0.42891895986162115 0.0 62-63 4.9505881793815925E-5 1.4851764538144777E-4 0.0 0.5001084178811285 0.0 64-65 4.9505881793815925E-5 1.4851764538144777E-4 0.0 0.5756048876166977 0.0 66-67 4.9505881793815925E-5 1.4851764538144777E-4 0.0 0.6881070039931445 0.0 68-69 4.9505881793815925E-5 1.4851764538144777E-4 0.0 0.8156094025531173 0.0 70-71 4.9505881793815925E-5 1.4851764538144777E-4 0.0 0.9296957071469661 0.0 72-73 4.9505881793815925E-5 1.4851764538144777E-4 0.0 1.0669507644203209 0.0 74-75 4.9505881793815925E-5 1.4851764538144777E-4 0.0 1.2259141508602638 4.9505881793815925E-5 76-77 4.9505881793815925E-5 1.4851764538144777E-4 0.0 1.4048284076631146 9.901176358763185E-5 78-79 4.9505881793815925E-5 1.4851764538144777E-4 0.0 1.6051292054008937 9.901176358763185E-5 80-81 4.9505881793815925E-5 1.4851764538144777E-4 0.0 1.8362226616144264 9.901176358763185E-5 82-83 7.425882269072389E-5 1.4851764538144777E-4 0.0 2.0890987058172383 9.901176358763185E-5 84-85 9.901176358763185E-5 1.980235271752637E-4 0.0 2.342667832365163 9.901176358763185E-5 86-87 9.901176358763185E-5 1.980235271752637E-4 0.0 2.6118313116781406 9.901176358763185E-5 88-89 9.901176358763185E-5 2.2277646807217165E-4 0.0 2.9229510258113764 9.901176358763185E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2915 0.0 53.979343 1 GTATCAA 5965 0.0 45.4258 1 ATCAACG 7140 0.0 37.66244 3 TCAACGC 7350 0.0 36.65374 4 CAACGCA 7460 0.0 36.30705 5 TATCAAC 7480 0.0 36.20458 2 AACGCAG 7765 0.0 34.93434 6 ACGCAGA 9105 0.0 29.376303 7 CGCAGAG 9140 0.0 28.95194 8 GCAGAGT 9945 0.0 25.55746 9 TGGTATC 1485 0.0 24.955048 2 GTACATG 6800 0.0 23.279491 1 TACATGG 6700 0.0 22.691616 2 GTGGTAT 1775 0.0 22.496716 1 GAGTACT 6080 0.0 21.760494 12-13 AGAGTAC 9220 0.0 21.125689 10-11 ACATGGG 6785 0.0 20.936863 3 CAGAGTA 9685 0.0 20.50381 10-11 GTACTTT 6415 0.0 20.43722 14-15 TAGACCG 140 4.8413425E-4 20.364704 5 >>END_MODULE