FastQCFastQC Report
Thu 26 May 2016
SRR937479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019962
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69630.3447094549303403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61460.30426314950479266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38520.19069665666977895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35550.056.1322561
GTATCAA66450.043.901181
ATCAACG84200.034.5233653
TCAACGC85650.033.9389084
TATCAAC86950.033.5407372
CAACGCA87400.033.205015
AACGCAG91000.032.0487826
TGGTATC15250.029.5888162
ACGCAGA107150.027.0416367
CGCAGAG108100.026.803998
GCAGAGT117150.024.2467889
GTGGTAT21500.023.8664671
GTACATG71300.022.6564671
GAGTACT72200.022.33533712-13
TACATGG72350.021.5332282
ACATGGG72500.021.1611043
GTACTTT75650.020.90783514-15
CAGAGTA114700.020.5406210-11
GTACCTG16050.020.4256971
AGTACTT73200.020.40795512-13
Produced by FastQC (version 0.11.5)