##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937479_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2019962 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.816759919246007 31.0 31.0 34.0 30.0 34.0 2 32.039985405666044 31.0 31.0 34.0 30.0 34.0 3 32.10737974278724 33.0 31.0 34.0 30.0 34.0 4 35.83070473602969 37.0 35.0 37.0 35.0 37.0 5 35.72275270524891 37.0 35.0 37.0 33.0 37.0 6 35.75385626066233 37.0 35.0 37.0 33.0 37.0 7 35.681362322657556 37.0 35.0 37.0 33.0 37.0 8 35.645977993645424 37.0 35.0 37.0 33.0 37.0 9 37.27449575784099 39.0 37.0 39.0 34.0 39.0 10-11 37.17115643759635 39.0 37.0 39.0 33.0 39.0 12-13 37.03339344997579 39.0 37.0 39.0 33.0 39.0 14-15 38.151246162056516 40.0 37.0 41.0 33.0 41.0 16-17 38.08103122732012 40.0 37.0 41.0 33.0 41.0 18-19 38.033855587382334 40.0 37.0 41.0 33.0 41.0 20-21 37.89951667407605 40.0 37.0 41.0 33.0 41.0 22-23 37.83611201596862 39.5 37.0 41.0 32.5 41.0 24-25 37.88079552981689 40.0 37.0 41.0 33.0 41.0 26-27 37.68623196872021 39.5 37.0 41.0 32.0 41.0 28-29 37.50938037448229 39.0 36.5 41.0 32.0 41.0 30-31 37.565159394087615 39.0 37.0 41.0 32.0 41.0 32-33 37.47134946102946 39.0 36.0 41.0 32.0 41.0 34-35 37.32098945425706 39.0 36.0 41.0 31.5 41.0 36-37 37.138158292086686 39.0 36.0 41.0 31.0 41.0 38-39 37.01016603282636 39.0 36.0 41.0 31.0 41.0 40-41 36.794863715258 39.0 35.0 40.0 31.0 41.0 42-43 36.57847350593724 39.0 35.0 40.0 30.0 41.0 44-45 36.270974404468994 39.0 35.0 40.0 30.0 41.0 46-47 35.93651291459938 38.0 35.0 40.0 29.5 41.0 48-49 35.60946369288135 38.0 35.0 40.0 28.0 41.0 50-51 35.33345899576329 38.0 34.0 40.0 27.0 41.0 52-53 35.04933508650163 38.0 34.0 40.0 27.0 41.0 54-55 34.79751104228693 38.0 34.0 40.0 26.0 41.0 56-57 34.437134708474716 38.0 33.0 40.0 25.5 41.0 58-59 34.12305627531607 37.0 33.0 40.0 25.0 41.0 60-61 33.71637907049737 37.0 32.5 39.5 23.5 41.0 62-63 33.26445373724852 36.0 32.0 39.0 22.5 40.0 64-65 32.782583533749644 36.0 31.0 39.0 22.0 40.0 66-67 32.273719010555645 35.0 31.0 38.5 20.5 40.0 68-69 31.753818883721575 35.0 30.5 38.0 18.5 40.0 70-71 31.259483346716422 34.5 30.0 37.5 18.0 39.5 72-73 30.70584644661632 34.0 29.0 37.0 14.0 39.0 74-75 30.16505558025349 34.0 29.0 36.0 10.5 39.0 76-77 28.945812842023763 32.5 27.0 35.0 9.0 37.0 78-79 29.59910730993949 34.0 29.0 35.0 7.5 37.0 80-81 29.38264581214894 34.0 29.0 35.0 7.0 37.0 82-83 28.89872160961444 33.5 28.5 35.0 2.0 36.5 84-85 28.353675960240835 33.0 27.0 35.0 2.0 36.0 86-87 27.834712484690307 33.0 26.0 35.0 2.0 36.0 88-89 27.231304598799383 32.0 26.0 34.5 2.0 35.0 90-91 26.496184334160745 32.0 25.0 34.0 2.0 35.0 92-93 25.790097288958904 31.5 23.5 34.0 2.0 35.0 94-95 25.067597558765957 31.0 20.0 34.0 2.0 35.0 96-97 24.145357684946546 31.0 12.5 34.0 2.0 35.0 98-99 22.854336121174555 30.5 2.0 34.0 2.0 35.0 100-101 19.8772907609153 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15389610625206984 1101 2 0.23554977120785736 1101 3 0.11213218975264283 1101 4 0.09515068037244845 1101 5 0.07764150984197471 1101 6 0.14505514749154713 1101 7 0.17029746381723498 1101 8 0.1343037735746151 1101 9 0.161631041416058 1101 10-11 0.1868933277412026 1101 12-13 0.19565802879866112 1101 14-15 0.28167314742101013 1101 16-17 0.18354129049588863 1101 18-19 0.1957139516656028 1101 20-21 0.29922411130041127 1101 22-23 0.3109786350679755 1101 24-25 0.1489289102093565 1101 26-27 0.19103855509742118 1101 28-29 0.11947153550610778 1101 30-31 0.32948988661864576 1101 32-33 0.28532638708216496 1101 34-35 0.3568080831595637 1101 36-37 0.44704166992887906 1101 38-39 0.3104899004178563 1101 40-41 0.41018424304649415 1101 42-43 0.48132008379560887 1101 44-45 0.38204191000175314 1101 46-47 0.46915943665685944 1101 48-49 0.4470991953253005 1101 50-51 0.5407865957154385 1101 52-53 0.5861463192660494 1101 54-55 0.5101452277005052 1101 56-57 0.526903953395113 1101 58-59 0.6552427593415544 1101 60-61 0.6447537994716441 1101 62-63 0.7255806471005073 1101 64-65 0.6001657231260609 1101 66-67 0.47458294891669794 1101 68-69 0.5230799705293521 1101 70-71 0.7318175259105253 1101 72-73 0.6424031447572958 1101 74-75 0.7766164978095205 1101 76-77 0.8691357368267774 1101 78-79 0.9860816386831353 1101 80-81 0.9393635845700423 1101 82-83 0.8962956891793432 1101 84-85 0.9582642775216641 1101 86-87 1.2279529112604664 1101 88-89 1.356237587265685 1101 90-91 1.5104356637191358 1101 92-93 1.543522057549719 1101 94-95 1.4985345855142214 1101 96-97 1.7608352274797987 1101 98-99 2.197868055574734 1101 100-101 2.9497405306068956 1102 1 0.25560099051509155 1102 2 0.23854987188237686 1102 3 0.22613175607779112 1102 4 0.14159570821282585 1102 5 0.0911582256842749 1102 6 0.1644679052763962 1102 7 0.12987161060068075 1102 8 0.0927356517739284 1102 9 0.19227675229432606 1102 10-11 0.09418680945329072 1102 12-13 0.18465585268020845 1102 14-15 0.2504053841764957 1102 16-17 0.31889024386810405 1102 18-19 0.28814477665456906 1102 20-21 0.28451304717074066 1102 22-23 0.18264437243139042 1102 24-25 0.0913347601733534 1102 26-27 0.07749047527027386 1102 28-29 -0.0031546754921976117 1102 30-31 0.1497454062927659 1102 32-33 0.2789531091972606 1102 34-35 0.2655764465790966 1102 36-37 0.28208221078337203 1102 38-39 0.26532460060139584 1102 40-41 0.3289195774795033 1102 42-43 0.2867119787223871 1102 44-45 0.35660339502835114 1102 46-47 0.4200260119420349 1102 48-49 0.4095417914888273 1102 50-51 0.41447860138419657 1102 52-53 0.27564228841318084 1102 54-55 0.3552630943300059 1102 56-57 0.4983282655171024 1102 58-59 0.3455423821993193 1102 60-61 0.46146054234117173 1102 62-63 0.5396835984127577 1102 64-65 0.4890481585201272 1102 66-67 0.723580463030487 1102 68-69 0.6437660673782375 1102 70-71 0.41888007576377717 1102 72-73 0.621499242458043 1102 74-75 0.4476539874516625 1102 76-77 0.3246468923379311 1102 78-79 0.499352282767731 1102 80-81 0.6547470383255884 1102 82-83 0.7651504778015727 1102 84-85 0.8133839640366993 1102 86-87 0.9583599073651357 1102 88-89 0.953578424722803 1102 90-91 1.174127785550791 1102 92-93 1.3313963925170782 1102 94-95 1.3739049515822614 1102 96-97 1.5442589742756354 1102 98-99 1.8412651623439338 1102 100-101 2.5634328460433977 1103 1 0.1146280829768358 1103 2 0.1624449231330516 1103 3 0.013446062844984397 1103 4 0.0698329218603746 1103 5 0.05496827581651331 1103 6 0.0065842489124960935 1103 7 0.10104754712616426 1103 8 -0.023155346791838838 1103 9 0.0034650574036092507 1103 10-11 0.11187256847643567 1103 12-13 0.09671653768922539 1103 14-15 0.04010919606876229 1103 16-17 0.08475097887777139 1103 18-19 0.10757990080093549 1103 20-21 0.24987114398781074 1103 22-23 0.11727245964306832 1103 24-25 0.053642816858307185 1103 26-27 -0.03111490111626125 1103 28-29 0.15664479555347555 1103 30-31 0.11824630762041721 1103 32-33 0.08491568765224855 1103 34-35 0.16115599367839906 1103 36-37 0.11487343172049691 1103 38-39 0.18038795669818342 1103 40-41 0.31575493057626147 1103 42-43 0.24542919868580526 1103 44-45 0.1821550470237696 1103 46-47 0.296406622213226 1103 48-49 0.43053877829127885 1103 50-51 0.28614993916495024 1103 52-53 0.31060261683855117 1103 54-55 0.39184120719991 1103 56-57 0.32322332992584535 1103 58-59 0.36290182113929603 1103 60-61 0.4462741583098051 1103 62-63 0.567650413765584 1103 64-65 0.49237057065712264 1103 66-67 0.36851592270758715 1103 68-69 0.6216710398120853 1103 70-71 0.5873829508016044 1103 72-73 0.4216288494265612 1103 74-75 0.45056981529150164 1103 76-77 0.4066453396859231 1103 78-79 0.5714749422764065 1103 80-81 0.6274901007343061 1103 82-83 0.5865185221179452 1103 84-85 0.6366941391705829 1103 86-87 0.7963630389852909 1103 88-89 1.004475147048094 1103 90-91 1.062354604324721 1103 92-93 1.1209258600100718 1103 94-95 1.0578553982986705 1103 96-97 1.17124288195863 1103 98-99 1.680347255529373 1103 100-101 2.3531195524474278 1104 1 0.04094529630138766 1104 2 0.07126172724616708 1104 3 0.10950565657876155 1104 4 0.05262703416127579 1104 5 -0.008601682915418962 1104 6 0.020278761087247688 1104 7 0.042531326575314665 1104 8 0.07941343071690454 1104 9 -0.051859406206467895 1104 10-11 0.052270530520438285 1104 12-13 0.10940495256743077 1104 14-15 0.06531772781811895 1104 16-17 0.12124451942680281 1104 18-19 0.05790126326188272 1104 20-21 0.14065736325981248 1104 22-23 0.1260427788132219 1104 24-25 -0.048754156434902995 1104 26-27 -0.001582937504799986 1104 28-29 -0.10201217158726195 1104 30-31 -0.02660549878741847 1104 32-33 0.024161767941834 1104 34-35 -0.0521848896905297 1104 36-37 0.030732455566216288 1104 38-39 0.1326787933699478 1104 40-41 0.1159198700046673 1104 42-43 0.24213013331181088 1104 44-45 0.0677894465458948 1104 46-47 -0.007792302340369872 1104 48-49 -0.07563279451449034 1104 50-51 -0.10803010537865987 1104 52-53 -0.13569195745145635 1104 54-55 0.05690270701933997 1104 56-57 -0.07170593600881148 1104 58-59 -0.11038548367330492 1104 60-61 0.025293486560855172 1104 62-63 -5.705305154108942E-4 1104 64-65 -0.09431139169025471 1104 66-67 -0.0727168860292906 1104 68-69 0.13630134057649457 1104 70-71 0.08079063297819644 1104 72-73 0.06199861417288588 1104 74-75 0.03588472540615584 1104 76-77 0.14707878825049292 1104 78-79 0.14016128959883645 1104 80-81 0.17072829463833727 1104 82-83 0.04933139817033805 1104 84-85 0.22258296025227153 1104 86-87 0.3942707736627433 1104 88-89 0.5116045606494914 1104 90-91 0.6574795166925753 1104 92-93 0.7726073914628842 1104 94-95 0.9052370633011151 1104 96-97 1.1358994700597798 1104 98-99 1.4506416054220388 1104 100-101 2.07008788529512 1105 1 0.006862656605655104 1105 2 0.0015031416797484098 1105 3 0.034838596343568895 1105 4 0.0702801627527947 1105 5 0.04382229165631912 1105 6 -0.012576849715131289 1105 7 -0.12604222630174888 1105 8 -0.03466326954134047 1105 9 -0.006196299790886428 1105 10-11 0.02972140582438243 1105 12-13 0.0775511280849912 1105 14-15 0.05611429292723358 1105 16-17 0.1160533469247369 1105 18-19 0.12672476447409764 1105 20-21 0.1727970276600459 1105 22-23 0.13022336715867056 1105 24-25 0.12956030733425905 1105 26-27 0.07693138079482509 1105 28-29 0.2179488091093873 1105 30-31 0.021368206812908852 1105 32-33 0.01541797512980736 1105 34-35 0.07282958634174008 1105 36-37 0.19219529161888715 1105 38-39 0.12996448121085535 1105 40-41 0.09019885586905474 1105 42-43 0.014530906731430093 1105 44-45 0.022184244259385366 1105 46-47 0.11911110513359091 1105 48-49 0.3289216300701838 1105 50-51 0.36362048189489116 1105 52-53 0.3464505886891658 1105 54-55 0.2946290515666803 1105 56-57 0.41456943879249764 1105 58-59 0.28438655450356265 1105 60-61 0.4902511644778045 1105 62-63 0.6672572710434608 1105 64-65 0.45294843230632864 1105 66-67 0.37091447671431155 1105 68-69 0.519011185589136 1105 70-71 0.5614028044081785 1105 72-73 0.45888751958016627 1105 74-75 0.71705687016253 1105 76-77 0.6176326982514979 1105 78-79 0.6035460400033124 1105 80-81 0.6136187538512985 1105 82-83 0.5388963559200732 1105 84-85 0.4685079908337286 1105 86-87 0.7584679953059279 1105 88-89 1.1492291196130395 1105 90-91 1.2354517805249756 1105 92-93 1.0895699257856712 1105 94-95 1.2500978967866132 1105 96-97 1.5171116180408255 1105 98-99 1.8174166829821452 1105 100-101 2.301650986162681 1106 1 -0.028075495600418776 1106 2 0.07106755744884197 1106 3 -0.03677342816885698 1106 4 0.020716181235734155 1106 5 0.10914999001749948 1106 6 -0.003307408840385051 1106 7 0.02370277943185073 1106 8 -0.12494463541855083 1106 9 -0.11637597709010805 1106 10-11 -0.2233390589872144 1106 12-13 -0.3626213619750587 1106 14-15 -0.07020621966680807 1106 16-17 -0.09876892623009326 1106 18-19 0.1406352739621397 1106 20-21 0.18845006543703136 1106 22-23 0.17127545679418432 1106 24-25 0.03336041744291407 1106 26-27 0.07128996846001456 1106 28-29 0.1497357267884496 1106 30-31 0.02122413082027208 1106 32-33 0.2233188044775929 1106 34-35 -0.26816075118249927 1106 36-37 0.10823045278527843 1106 38-39 0.23147521827640105 1106 40-41 0.08582882676680725 1106 42-43 0.1163511403737445 1106 44-45 0.07828933104355684 1106 46-47 0.23584007592683776 1106 48-49 0.2909495097867847 1106 50-51 0.4558192729077959 1106 52-53 0.4376750727020635 1106 54-55 0.2352625664026462 1106 56-57 0.3463102631942192 1106 58-59 0.3000099674595802 1106 60-61 0.37045510468939824 1106 62-63 0.469263368236426 1106 64-65 0.5253299661433957 1106 66-67 0.47840307943847193 1106 68-69 0.5855040042381319 1106 70-71 0.6504237353562168 1106 72-73 0.5633097693295497 1106 74-75 0.5440957570641629 1106 76-77 0.3604416869741911 1106 78-79 0.4922516598134514 1106 80-81 0.4768091225827966 1106 82-83 0.41289490816795293 1106 84-85 0.5225157874977917 1106 86-87 0.7366013813383177 1106 88-89 0.8620846542656153 1106 90-91 0.7451960465580747 1106 92-93 0.9089542005626932 1106 94-95 0.5976748808399961 1106 96-97 1.2397676322842948 1106 98-99 1.3529429154851051 1106 100-101 1.6138670662946915 1107 1 0.037737752376511224 1107 2 0.0380887977041553 1107 3 0.05710608004335427 1107 4 0.09334871115481036 1107 5 0.014696848096484416 1107 6 0.06539926428034448 1107 7 0.018972163781334928 1107 8 -0.047616194960895086 1107 9 0.04778065598346615 1107 10-11 -0.06831014290599313 1107 12-13 0.07082958716433296 1107 14-15 0.07126208275534651 1107 16-17 0.05939189907564213 1107 18-19 0.13468861662769172 1107 20-21 0.17201446940307363 1107 22-23 0.07819725509315845 1107 24-25 0.01010199627475572 1107 26-27 -0.02029355129973709 1107 28-29 0.030334697045987014 1107 30-31 0.002012580086848459 1107 32-33 0.11863555857934216 1107 34-35 0.09579793556153504 1107 36-37 -4.9133050097793785E-5 1107 38-39 0.025858059632980712 1107 40-41 0.014996329263389896 1107 42-43 0.010054197621549577 1107 44-45 0.13392298824281568 1107 46-47 0.11917402704749946 1107 48-49 0.1875096517470496 1107 50-51 0.12176503626762525 1107 52-53 0.02573547849384994 1107 54-55 0.10299375332473915 1107 56-57 0.013150364569483486 1107 58-59 0.1296383029660788 1107 60-61 0.34496129273804854 1107 62-63 0.3068056503283003 1107 64-65 0.4429380316840934 1107 66-67 0.541125392027233 1107 68-69 0.3778075369641414 1107 70-71 0.43652686368590565 1107 72-73 0.4605380301201123 1107 74-75 0.37198093293004675 1107 76-77 0.34784309538395064 1107 78-79 0.27951986237210846 1107 80-81 0.44330578871162274 1107 82-83 0.6046151224602099 1107 84-85 0.8050551005764213 1107 86-87 1.015982801784027 1107 88-89 1.2071860186307681 1107 90-91 1.2356622252454272 1107 92-93 1.1514742495786656 1107 94-95 1.3559661319538314 1107 96-97 1.4668930569222347 1107 98-99 1.7680589359462182 1107 100-101 2.1484845069398624 1108 1 0.003871052363294325 1108 2 -0.043649084868246035 1108 3 -0.10136093341318997 1108 4 -0.0854611233700524 1108 5 -0.060784280997772555 1108 6 -0.1464754923450542 1108 7 -0.16333211138186243 1108 8 -0.017993698579672923 1108 9 -0.0957147893265855 1108 10-11 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2316 60-61 0.2577910275644939 2316 62-63 0.31720826206233 2316 64-65 0.6232380755347435 2316 66-67 0.506051682371826 2316 68-69 0.6690374366524452 2316 70-71 0.5151967352103135 2316 72-73 0.6065783105392448 2316 74-75 0.7447087897584161 2316 76-77 0.7289381062104106 2316 78-79 0.8304619703359002 2316 80-81 0.8650048429765782 2316 82-83 0.8666060248568392 2316 84-85 1.0180968641009152 2316 86-87 0.9936952131759575 2316 88-89 1.1564935129466214 2316 90-91 1.442637735501478 2316 92-93 1.5210459551616715 2316 94-95 1.8159843176857606 2316 96-97 1.98560434525195 2316 98-99 1.9509032649557803 2316 100-101 2.0788728590703514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 12.0 4 28.0 5 51.0 6 113.0 7 319.0 8 711.0 9 1363.0 10 2137.0 11 3404.0 12 4980.0 13 6638.0 14 8726.0 15 11907.0 16 15324.0 17 19667.0 18 18653.0 19 14548.0 20 14615.0 21 15481.0 22 16586.0 23 19187.0 24 22210.0 25 26324.0 26 32067.0 27 39628.0 28 48185.0 29 58451.0 30 70842.0 31 86320.0 32 106955.0 33 134211.0 34 171747.0 35 226917.0 36 300805.0 37 343144.0 38 170063.0 39 7637.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.38476160006022 20.50299408315087 15.497519004293391 22.614725312495512 2 16.105699018100342 21.42055147572083 38.88885038431416 23.58489912186467 3 19.5995766256989 24.732891014781465 30.0570010722974 25.610531287222233 4 13.609909493346905 17.658550012326966 36.67009577407892 32.06144472024721 5 13.699464289162066 37.491441547813714 34.34217867622342 14.466915486800799 6 31.817281711240113 36.908268571388966 17.18170935888893 14.092740358481992 7 28.369989138409537 32.71546692462531 20.721181883619593 18.193362053345556 8 26.184849021912292 35.976171828975 19.494574650414215 18.34440449869849 9 25.787712838162303 16.985715572867214 20.269044665196674 36.95752692377381 10-11 24.765144096770136 27.28095875070917 27.207591033890736 20.746306118629953 12-13 25.48318670908245 24.27559434620123 28.08717300407383 22.15404594064249 14-15 22.50512633405975 25.878209590081397 26.129130151953355 25.4875339239055 16-17 21.97454704593453 29.141538306166154 26.828326473468312 22.055588174431005 18-19 21.883802764606465 27.63160891145477 29.160053505957045 21.324534817981725 20-21 22.557867597115976 26.692239830966614 29.499528702605403 21.250363869312004 22-23 22.326009221024886 26.558838861773243 29.32960188598821 21.78555003121366 24-25 22.092778028882737 26.838463712913267 29.376557270165115 21.692200988038877 26-27 21.98323299102588 27.11121089509035 29.22171631634734 21.683839797536436 28-29 21.94006637508234 27.18006895938852 29.2617721458826 21.618092519646545 30-31 22.067639608892375 26.931038827340505 29.33661162337487 21.664709940392253 32-33 21.96739831590474 26.873245321558827 29.229310326902997 21.93004603563343 34-35 22.23789358413673 26.981720448206453 29.208123717178836 21.57226225047798 36-37 22.273375575072983 26.862919630626532 29.153533162273927 21.710171632026558 38-39 22.090753754838275 27.072435327324467 29.13165876263422 21.705152155203034 40-41 22.312969253877053 27.00184458915564 29.015075531123852 21.670110625843456 42-43 22.12507525527381 26.98008012706259 28.913422768285052 21.98142184937855 44-45 22.198363137524368 27.115460587872448 28.90086546182552 21.785310812777666 46-47 22.24576501934195 27.365663314458388 28.39956890278134 21.989002763418323 48-49 22.383391370728756 27.38744590244767 28.27917059825878 21.949992128564794 50-51 22.4750762638109 27.493388489486435 28.176346881773025 21.85518836492964 52-53 22.519760896432928 27.5933476949932 27.868006395171392 22.018885013402485 54-55 22.397871841153446 27.545344912429048 27.985947260394006 22.0708359860235 56-57 22.337202383015125 27.775002698070555 27.980526366337582 21.90726855257673 58-59 22.40047089994762 27.482076395496545 28.14788099974158 21.96957170481425 60-61 22.40826807633015 27.513982936312665 28.22414481064495 21.853604176712235 62-63 22.26257226621095 27.525666324416004 28.336671679962294 21.875089729410753 64-65 22.176630055416886 27.462001760429157 28.2461749280432 22.115193256110757 66-67 22.27665668958129 27.572177100361294 28.01946274236842 22.131703467688997 68-69 22.125539985405666 27.544899359492902 28.18013408173025 22.14942657337118 70-71 22.294775842317826 27.372346608500557 28.10406829435405 22.228809254827567 72-73 22.23699700686846 27.45663523697027 27.996176158118143 22.310191598043126 74-75 22.17578845542639 27.766264909933952 27.66883733456372 22.389109300075944 76-77 22.23153207832623 27.45885813693525 27.95215949606973 22.357450288668794 78-79 22.205987043320615 27.48175460726489 27.882826508617487 22.429431840797005 80-81 22.1460601734092 27.73688316908932 27.777279968633074 22.339776688868405 82-83 22.302936481395715 27.57534029901213 27.712521810548424 22.40920140904373 84-85 22.252099118025125 27.52654699571352 27.789473579987284 22.431880306274074 86-87 22.11400511494771 27.50430453642197 27.882034414508787 22.49965593412153 88-89 22.18172916124165 27.424476302029444 27.805646838900934 22.588147697827978 90-91 22.252033421991257 27.484097025965614 27.75351182424738 22.510357727795746 92-93 22.112721727980674 27.40862307109652 27.828142559395623 22.650512641527182 94-95 22.09846026806445 27.418411831509705 27.723417569241406 22.75971033118445 96-97 22.030067892366294 27.39009446712364 27.733467263245547 22.84637037726452 98-99 21.942799885740367 27.37649390260505 27.661796440624347 23.018909771030234 100-101 22.16643573354419 27.079505937626024 27.99657078547877 22.757487543351022 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 367.0 1 267.0 2 208.5 3 278.5 4 253.5 5 245.0 6 469.0 7 996.5 8 1599.5 9 2015.5 10 2023.0 11 1841.5 12 1928.5 13 2107.5 14 2324.0 15 2558.0 16 2653.0 17 2766.0 18 2978.0 19 3168.0 20 3536.5 21 4112.5 22 4740.5 23 5749.5 24 7081.0 25 8910.5 26 11329.0 27 14316.0 28 17210.5 29 20351.5 30 24471.0 31 27955.0 32 31422.5 33 35360.0 34 39812.5 35 44609.5 36 50248.5 37 55429.0 38 58730.5 39 61049.5 40 63070.0 41 66400.0 42 69404.5 43 72053.5 44 74958.0 45 76834.5 46 78721.5 47 80631.0 48 80568.5 49 79347.5 50 77497.5 51 73517.0 52 69506.5 53 67571.0 54 65498.0 55 60473.5 56 53530.0 57 46432.0 58 40892.5 59 36187.5 60 30178.5 61 24200.0 62 19079.5 63 14614.0 64 10813.0 65 7902.0 66 5841.5 67 4513.0 68 3511.5 69 2528.0 70 1779.5 71 1319.5 72 967.5 73 746.5 74 569.5 75 407.0 76 267.0 77 139.5 78 73.5 79 47.5 80 31.0 81 21.0 82 13.5 83 5.5 84 2.5 85 1.5 86 2.0 87 1.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040248281898372346 2 0.0 3 0.0 4 0.0 5 0.0014356705720206617 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005767435228979555 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.9703529076289555E-4 22-23 6.435764633196069E-4 24-25 4.9505881793815925E-5 26-27 0.002128752917134085 28-29 0.003836705839020734 30-31 3.2178823165980347E-4 32-33 2.4752940896907965E-4 34-35 0.0 36-37 4.9505881793815925E-5 38-39 7.425882269072389E-5 40-41 0.0 42-43 0.008415999904948707 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.4752940896907962E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.980235271752637E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.198117588350672E-4 84-85 3.2178823165980347E-4 86-87 0.0 88-89 0.0 90-91 0.010594258703876609 92-93 0.0015841882174021096 94-95 0.0 96-97 0.0 98-99 4.9505881793815925E-5 100-101 0.0127477645619076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2019962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.83352998277607 #Duplication Level Percentage of deduplicated Percentage of total 1 79.67536928728111 48.46933966426572 2 11.899812811343915 14.478152388966256 3 3.571932211676112 6.518797358863273 4 1.539301385235979 3.7456454788512663 5 0.8376188128802666 2.54776545837445 6 0.5288585151747304 1.930339820371704 7 0.3277338816716523 1.3956046241930828 8 0.25542458889119535 1.2430703509320626 9 0.1981990987462071 1.0851435734522925 >10 1.069391916973587 11.801277769384841 >50 0.0681440845669064 2.8166281726302267 >100 0.02739855420463785 2.836149187806241 >500 4.074256768685354E-4 0.15109022642164363 >1k 2.4445540612111057E-4 0.3311790691348306 >5k 1.6297027074740704E-4 0.6498168563521172 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6963 0.3447094549303403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6146 0.30426314950479266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3852 0.19069665666977895 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.901176358763185E-5 0.0 18-19 0.0 0.0 0.0 1.4851764538144777E-4 0.0 20-21 0.0 0.0 0.0 1.4851764538144777E-4 0.0 22-23 0.0 0.0 0.0 1.980235271752637E-4 0.0 24-25 0.0 0.0 0.0 4.207999952474354E-4 0.0 26-27 0.0 0.0 0.0 8.168470495979628E-4 0.0 28-29 0.0 0.0 0.0 0.0018069646854742812 0.0 30-31 0.0 0.0 0.0 0.004430776420546525 0.0 32-33 0.0 0.0 0.0 0.008440752845845615 0.0 34-35 0.0 0.0 0.0 0.012698258680113785 0.0 36-37 0.0 0.0 0.0 0.017995388032052088 0.0 38-39 0.0 0.0 0.0 0.026163858528031714 0.0 40-41 0.0 0.0 0.0 0.037574964281506286 0.0 42-43 0.0 0.0 0.0 0.05230296411516652 0.0 44-45 0.0 0.0 0.0 0.06849138746174432 0.0 46-47 0.0 0.0 0.0 0.08678381078455932 0.0 48-49 0.0 0.0 0.0 0.10621486938863206 0.0 50-51 0.0 0.0 0.0 0.13396291613406588 0.0 52-53 0.0 0.0 0.0 0.17698352741289192 0.0 54-55 0.0 0.0 0.0 0.23027660916393478 0.0 56-57 0.0 0.0 0.0 0.2817132203477095 0.0 58-59 0.0 0.0 0.0 0.3500313372231755 0.0 60-61 0.0 0.0 0.0 0.430032842201982 0.0 62-63 0.0 0.0 0.0 0.5012470531623863 0.0 64-65 0.0 0.0 0.0 0.5769910523069246 0.0 66-67 0.0 0.0 0.0 0.689047615747227 0.0 68-69 0.0 0.0 0.0 0.8165747672480967 0.0 70-71 0.0 0.0 0.0 0.9314036600688528 0.0 72-73 0.0 0.0 0.0 1.0682874232287538 0.0 74-75 0.0 0.0 0.0 1.2291072802359648 0.0 76-77 0.0 0.0 0.0 1.4096057252562177 0.0 78-79 0.0 0.0 0.0 1.6126293464926569 0.0 80-81 0.0 0.0 0.0 1.8459505673869114 0.0 82-83 0.0 0.0 0.0 2.1006583292160945 0.0 84-85 0.0 0.0 0.0 2.356777008676401 0.0 86-87 0.0 0.0 0.0 2.630544534996203 0.0 88-89 0.0 0.0 0.0 2.945971260845501 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3555 0.0 56.132256 1 GTATCAA 6645 0.0 43.90118 1 ATCAACG 8420 0.0 34.523365 3 TCAACGC 8565 0.0 33.938908 4 TATCAAC 8695 0.0 33.540737 2 CAACGCA 8740 0.0 33.20501 5 AACGCAG 9100 0.0 32.048782 6 TGGTATC 1525 0.0 29.588816 2 ACGCAGA 10715 0.0 27.041636 7 CGCAGAG 10810 0.0 26.80399 8 GCAGAGT 11715 0.0 24.246788 9 GTGGTAT 2150 0.0 23.866467 1 GTACATG 7130 0.0 22.656467 1 GAGTACT 7220 0.0 22.335337 12-13 TACATGG 7235 0.0 21.533228 2 ACATGGG 7250 0.0 21.161104 3 GTACTTT 7565 0.0 20.907835 14-15 CAGAGTA 11470 0.0 20.54062 10-11 GTACCTG 1605 0.0 20.425697 1 AGTACTT 7320 0.0 20.407955 12-13 >>END_MODULE